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Raj Boopathy - One of the best experts on this subject based on the ideXlab platform.

  • Presence of Methicillin Resistant Staphylococcus aureus (MRSA) in Sewage Treatment Plant.
    Bioresource technology, 2017
    Co-Authors: Raj Boopathy
    Abstract:

    The presence of antibiotic resistant bacteria and antibiotic resistance genes in rural Sewage Treatment Plants are not well reported in the literature. The aim of the present study was to study the frequency occurrence of Methicillin Resistant Staphylococcus aureus (MRSA) in a rural Sewage Treatment Plant. This study was conducted using raw Sewage as well as treated Sewage from a small town Sewage Treatment Plant in rural southeast Louisiana of USA. Results showed the presence of MRSA consistently in both raw and treated Sewage. The presence of mecA gene responsible for methicillin resistance was confirmed in the raw and treated Sewage water samples.

  • presence of antibiotic resistance genes in a Sewage Treatment Plant in thibodaux louisiana usa
    Bioresource Technology, 2015
    Co-Authors: Anthony Naquin, Mingma Sherpa, Arsen Shrestha, Rajkumar Nathaniel, Raj Boopathy
    Abstract:

    Increasing uses and disposals of antibiotics to the environment have increased emergence of various antibiotic resistance. One of the sources for the spread of antibiotic resistance is wastewater Treatment Plant, where bacteria and antibiotics can come in contact and can acquire antibiotics resistance. There are very few studies on this subject from a small town Sewage Treatment Plant. Therefore, this study was conducted using raw Sewage as well as treated Sewage from a Sewage Treatment Plant in Thibodaux in rural southeast Louisiana in USA. Samples were collected monthly from the Thibodaux Sewage Treatment Plant and the presence of antibiotic resistance genes was monitored. The study showed the presence of antibiotic resistance genes in both raw and treated Sewage in every month of the study period. The genetic transformation assay showed the successful transformation of methicillin resistant gene, mecA to an antibiotic sensitive Staphylococcus aureus, which became antibiotic resistant within 24h.

  • Presence of Antibiotic Resistant Staphylococcus aureus in Sewage Treatment Plant
    2014
    Co-Authors: Anthony Naquin, Jacob Clement, Mary Sauce, Richard Grabert, Mingma Sherpa, Raj Boopathy
    Abstract:

    Antibiotic resistance has become very common in the world. After passing through the human or animal body the antibiotics are entered into the Sewage Treatment Plant, where water is processed and cleaned then returned into the environment. During the Sewage Treatment process, antibiotics come into contact with bacteria entering the Treatment process, as well as bacteria used in the Treatment process. The bacteria that are exposed to these antibiotics can become resistant during the Treatment process and then expose the antibiotic resistance genes (ARGs) to the environment upon release of treated water from the Treatment Plant. Because of the contact between bacteria and antibiotics during the Treatment process, Sewage Treatment Plants are considered prime habitat to create antibiotic resistant bacteria. There are very limited studies on this subject from a small town Sewage Treatment Plant. Therefore, this study was conducted using raw Sewage as well as treated Sewage from Thibodaux Sewage Treatment Plant, which serves 15,000 people in rural southeast Louisiana of USA. Samples were collected monthly from Thibodaux Sewage Treatment Plant and antibiotic resistance was monitored using Kirby-Bauer assay. Special attention was given to Methicillin Resistant Staphylococcus aureus (MRSA) in raw and treated Sewage samples for the five month of the study period. Results showed the presence of MRSA consistently in both raw and treated Sewage. The presence of mecA gene responsible for Methicillin resistance was confirmed in isolates of pure culture of S. aureus as well as in the Sewage samples.

Anthony Naquin - One of the best experts on this subject based on the ideXlab platform.

  • presence of antibiotic resistance genes in a Sewage Treatment Plant in thibodaux louisiana usa
    Bioresource Technology, 2015
    Co-Authors: Anthony Naquin, Mingma Sherpa, Arsen Shrestha, Rajkumar Nathaniel, Raj Boopathy
    Abstract:

    Increasing uses and disposals of antibiotics to the environment have increased emergence of various antibiotic resistance. One of the sources for the spread of antibiotic resistance is wastewater Treatment Plant, where bacteria and antibiotics can come in contact and can acquire antibiotics resistance. There are very few studies on this subject from a small town Sewage Treatment Plant. Therefore, this study was conducted using raw Sewage as well as treated Sewage from a Sewage Treatment Plant in Thibodaux in rural southeast Louisiana in USA. Samples were collected monthly from the Thibodaux Sewage Treatment Plant and the presence of antibiotic resistance genes was monitored. The study showed the presence of antibiotic resistance genes in both raw and treated Sewage in every month of the study period. The genetic transformation assay showed the successful transformation of methicillin resistant gene, mecA to an antibiotic sensitive Staphylococcus aureus, which became antibiotic resistant within 24h.

  • Presence of Antibiotic Resistant Staphylococcus aureus in Sewage Treatment Plant
    2014
    Co-Authors: Anthony Naquin, Jacob Clement, Mary Sauce, Richard Grabert, Mingma Sherpa, Raj Boopathy
    Abstract:

    Antibiotic resistance has become very common in the world. After passing through the human or animal body the antibiotics are entered into the Sewage Treatment Plant, where water is processed and cleaned then returned into the environment. During the Sewage Treatment process, antibiotics come into contact with bacteria entering the Treatment process, as well as bacteria used in the Treatment process. The bacteria that are exposed to these antibiotics can become resistant during the Treatment process and then expose the antibiotic resistance genes (ARGs) to the environment upon release of treated water from the Treatment Plant. Because of the contact between bacteria and antibiotics during the Treatment process, Sewage Treatment Plants are considered prime habitat to create antibiotic resistant bacteria. There are very limited studies on this subject from a small town Sewage Treatment Plant. Therefore, this study was conducted using raw Sewage as well as treated Sewage from Thibodaux Sewage Treatment Plant, which serves 15,000 people in rural southeast Louisiana of USA. Samples were collected monthly from Thibodaux Sewage Treatment Plant and antibiotic resistance was monitored using Kirby-Bauer assay. Special attention was given to Methicillin Resistant Staphylococcus aureus (MRSA) in raw and treated Sewage samples for the five month of the study period. Results showed the presence of MRSA consistently in both raw and treated Sewage. The presence of mecA gene responsible for Methicillin resistance was confirmed in isolates of pure culture of S. aureus as well as in the Sewage samples.

Mingma Sherpa - One of the best experts on this subject based on the ideXlab platform.

  • presence of antibiotic resistance genes in a Sewage Treatment Plant in thibodaux louisiana usa
    Bioresource Technology, 2015
    Co-Authors: Anthony Naquin, Mingma Sherpa, Arsen Shrestha, Rajkumar Nathaniel, Raj Boopathy
    Abstract:

    Increasing uses and disposals of antibiotics to the environment have increased emergence of various antibiotic resistance. One of the sources for the spread of antibiotic resistance is wastewater Treatment Plant, where bacteria and antibiotics can come in contact and can acquire antibiotics resistance. There are very few studies on this subject from a small town Sewage Treatment Plant. Therefore, this study was conducted using raw Sewage as well as treated Sewage from a Sewage Treatment Plant in Thibodaux in rural southeast Louisiana in USA. Samples were collected monthly from the Thibodaux Sewage Treatment Plant and the presence of antibiotic resistance genes was monitored. The study showed the presence of antibiotic resistance genes in both raw and treated Sewage in every month of the study period. The genetic transformation assay showed the successful transformation of methicillin resistant gene, mecA to an antibiotic sensitive Staphylococcus aureus, which became antibiotic resistant within 24h.

  • Presence of Antibiotic Resistant Staphylococcus aureus in Sewage Treatment Plant
    2014
    Co-Authors: Anthony Naquin, Jacob Clement, Mary Sauce, Richard Grabert, Mingma Sherpa, Raj Boopathy
    Abstract:

    Antibiotic resistance has become very common in the world. After passing through the human or animal body the antibiotics are entered into the Sewage Treatment Plant, where water is processed and cleaned then returned into the environment. During the Sewage Treatment process, antibiotics come into contact with bacteria entering the Treatment process, as well as bacteria used in the Treatment process. The bacteria that are exposed to these antibiotics can become resistant during the Treatment process and then expose the antibiotic resistance genes (ARGs) to the environment upon release of treated water from the Treatment Plant. Because of the contact between bacteria and antibiotics during the Treatment process, Sewage Treatment Plants are considered prime habitat to create antibiotic resistant bacteria. There are very limited studies on this subject from a small town Sewage Treatment Plant. Therefore, this study was conducted using raw Sewage as well as treated Sewage from Thibodaux Sewage Treatment Plant, which serves 15,000 people in rural southeast Louisiana of USA. Samples were collected monthly from Thibodaux Sewage Treatment Plant and antibiotic resistance was monitored using Kirby-Bauer assay. Special attention was given to Methicillin Resistant Staphylococcus aureus (MRSA) in raw and treated Sewage samples for the five month of the study period. Results showed the presence of MRSA consistently in both raw and treated Sewage. The presence of mecA gene responsible for Methicillin resistance was confirmed in isolates of pure culture of S. aureus as well as in the Sewage samples.

A. Rajbanshi - One of the best experts on this subject based on the ideXlab platform.

  • Study on Heavy Metal Resistant Bacteria in Guheswori Sewage Treatment Plant
    Our Nature, 2009
    Co-Authors: A. Rajbanshi
    Abstract:

    Removal of heavy metals from wastewater needs advance chemical technology and is more expensive too. The cheaper alternative for this is the bioremediation using heavy metals resistant microorganisms. In this study, 10 heavy metal resistant bacteria were isolated from oxidation ditch of wastewater Treatment Plant of Bagmati Area Sewerage Project. These include chromium resistant Staphylococcus spp, Escherichia coli , Klebsiella spp; cadmium resistant Acinetobacter spp, Flavobacterium spp, Citrobacter spp; nickel resistant Staphylococcus spp, Bacillus spp; copper resistant Pseudomonas spp; and cobalt resistant Methylobacterium spp. All the isolates showed high resistance to heavy metals with Minimum Inhibitor Concentration (MIC) for heavy metals ranging from 150 mug/ml to 500 mug/ml. Six resistant isolates showed multiple tolerance to heavy metals. All the 10 isolates also showed antibiotic resistance of which 10% were resistant to single antibiotic and 90% were multi-antibiotic resistant. Heavy metal tolerance test showed maximum microbial tolerance to chromium and minimum tolerance to nickel in mixed liquor sample of oxidation ditch. Keywords: Heavy metal resistant bacteria, multiple tolerance, antibiotic resistance, Guheswori Sewage Treatment Plant doi: 10.3126/on.v6i1.1655 Our Nature (2008)6:52-57

Rajkumar Nathaniel - One of the best experts on this subject based on the ideXlab platform.

  • presence of antibiotic resistance genes in a Sewage Treatment Plant in thibodaux louisiana usa
    Bioresource Technology, 2015
    Co-Authors: Anthony Naquin, Mingma Sherpa, Arsen Shrestha, Rajkumar Nathaniel, Raj Boopathy
    Abstract:

    Increasing uses and disposals of antibiotics to the environment have increased emergence of various antibiotic resistance. One of the sources for the spread of antibiotic resistance is wastewater Treatment Plant, where bacteria and antibiotics can come in contact and can acquire antibiotics resistance. There are very few studies on this subject from a small town Sewage Treatment Plant. Therefore, this study was conducted using raw Sewage as well as treated Sewage from a Sewage Treatment Plant in Thibodaux in rural southeast Louisiana in USA. Samples were collected monthly from the Thibodaux Sewage Treatment Plant and the presence of antibiotic resistance genes was monitored. The study showed the presence of antibiotic resistance genes in both raw and treated Sewage in every month of the study period. The genetic transformation assay showed the successful transformation of methicillin resistant gene, mecA to an antibiotic sensitive Staphylococcus aureus, which became antibiotic resistant within 24h.