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Marta D. Mudry - One of the best experts on this subject based on the ideXlab platform.
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meiotic characterization and sex determination System of neotropical primates bolivian squirrel monkey saimiri boliviensis primates cebidae
American Journal of Primatology, 2007Co-Authors: Eliana Ruth Steinberg, Mariela Nieves, Marta D. MudryAbstract:The chromosomal sex determination System differs among platyrrhine monkeys more than any other group of primates. Although a number of studies have investigated mitotic chromosomes across platyrrhine species, the meiotic chromosomes of many genera have not yet been described. The goal of this study was to characterize the sex determination System of Saimiri boliviensis. We described for the first time the meiotic cycle, confirming the sexual System in germ cells from testicular biopsies of four adult male S. boliviensis. All specimens were weighed and testicular volume was measured. We observed 22 bivalents corresponding to 2N = 44, and a "human-like" XY bivalent was found in diakinesis/metaphase I. In addition, mitotic studies from blood samples of both sexes were performed and G- and C-banding patterns agreed with previously reported karylogy of S. boliviensis boliviensis. Further meiotic studies should be performed in New World primates based on the great value of those studies for Systematic evolutionary biology and conservation programs.
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RESEARCH ARTICLE Meiotic Characterization and Sex Determination System of Neotropical Primates: Bolivian Squirrel Monkey Saimiri boliviensis (Primates: Cebidae)
2007Co-Authors: Eliana Ruth Steinberg, Mariela Nieves, Marta D. MudryAbstract:The chromosomal sex determination System differs among platyrrhine monkeys more than any other group of primates. Although a number of studies have investigated mitotic chromosomes across platyrrhine species, the meiotic chromosomes of many genera have not yet been described. The goal of this study was to characterize the sex determination System of Saimiri boliviensis. We described for the first time the meiotic cycle, confirming the sexual System in germ cells from testicular biopsies of four adult male S. boliviensis. All specimens were weighed and testicular volume was measured. We observed 22 bivalents corresponding to 2N 544, and a ‘‘human-like’’ XY bivalent was found in diakinesis/ metaphase I. In addition, mitotic studies from blood samples of both sexes were performed and G- and C-banding patterns agreed with previously reported karylogy of S. boliviensis boliviensis. Further meiotic studies should be performed in New World primates based on the great value of those studies for Systematic evolutionary biology and conservation
Eliana Ruth Steinberg - One of the best experts on this subject based on the ideXlab platform.
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meiotic characterization and sex determination System of neotropical primates bolivian squirrel monkey saimiri boliviensis primates cebidae
American Journal of Primatology, 2007Co-Authors: Eliana Ruth Steinberg, Mariela Nieves, Marta D. MudryAbstract:The chromosomal sex determination System differs among platyrrhine monkeys more than any other group of primates. Although a number of studies have investigated mitotic chromosomes across platyrrhine species, the meiotic chromosomes of many genera have not yet been described. The goal of this study was to characterize the sex determination System of Saimiri boliviensis. We described for the first time the meiotic cycle, confirming the sexual System in germ cells from testicular biopsies of four adult male S. boliviensis. All specimens were weighed and testicular volume was measured. We observed 22 bivalents corresponding to 2N = 44, and a "human-like" XY bivalent was found in diakinesis/metaphase I. In addition, mitotic studies from blood samples of both sexes were performed and G- and C-banding patterns agreed with previously reported karylogy of S. boliviensis boliviensis. Further meiotic studies should be performed in New World primates based on the great value of those studies for Systematic evolutionary biology and conservation programs.
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RESEARCH ARTICLE Meiotic Characterization and Sex Determination System of Neotropical Primates: Bolivian Squirrel Monkey Saimiri boliviensis (Primates: Cebidae)
2007Co-Authors: Eliana Ruth Steinberg, Mariela Nieves, Marta D. MudryAbstract:The chromosomal sex determination System differs among platyrrhine monkeys more than any other group of primates. Although a number of studies have investigated mitotic chromosomes across platyrrhine species, the meiotic chromosomes of many genera have not yet been described. The goal of this study was to characterize the sex determination System of Saimiri boliviensis. We described for the first time the meiotic cycle, confirming the sexual System in germ cells from testicular biopsies of four adult male S. boliviensis. All specimens were weighed and testicular volume was measured. We observed 22 bivalents corresponding to 2N 544, and a ‘‘human-like’’ XY bivalent was found in diakinesis/ metaphase I. In addition, mitotic studies from blood samples of both sexes were performed and G- and C-banding patterns agreed with previously reported karylogy of S. boliviensis boliviensis. Further meiotic studies should be performed in New World primates based on the great value of those studies for Systematic evolutionary biology and conservation
Mhd Ikhwanuddin - One of the best experts on this subject based on the ideXlab platform.
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High-Density Genetic Linkage Maps Provide Novel Insights Into ZW/ZZ Sex Determination System and Growth Performance in Mud Crab (Scylla paramamosain).
Frontiers in genetics, 2019Co-Authors: Khor Waiho, Xi Shi, Yueling Zhang, Huaiping Zheng, Wenhua Liu, Shaobin Fang, Hanafiah Fazhan, Mhd IkhwanuddinAbstract:Mud crab, Scylla paramamosain is one of the most important crustacean species in global aquaculture. To determine the genetic basis of sex and growth-related traits in S. paramamosain, a high-density genetic linkage map with 16,701 single nucleotide polymorphisms (SNPs) was constructed using SLAF-seq and a full-sib family. The consensus map has 49 linkage groups, spanning 5,996.66 cM with an average marker-interval of 0.81 cM. A total of 516 SNP markers, including 8 female-specific SNPs segregated in two quantitative trait loci (QTLs) for phenotypic sex were located on LG32. The presence of female-specific SNP markers only on female linkage map, their segregation patterns and lower female: male recombination rate strongly suggest the conformation of a ZW/ZZ sex determination System in S. paramamosain. The QTLs of most (90%) growth-related traits were found within a small interval (25.18-33.74 cM) on LG46, highlighting the potential involvement of LG46 in growth. Four markers on LG46 were significantly associated with 10-16 growth-related traits. BW was only associated with marker 3846. Based on the annotation of transcriptome data, 11 and 2 candidate genes were identified within the QTL regions of sex and growth-related traits, respectively. The newly constructed high-density genetic linkage map with sex-specific SNPs, and the identified QTLs of sex- and growth-related traits serve as a valuable genetic resource and solid foundation for marker-assisted selection and genetic improvement of crustaceans.
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Identification of male-specific SNP markers and development of PCR-based genetic sex identification technique in crucifix crab (Charybdis feriatus) with implication of an XX/XY sex determination System
Genomics, 2019Co-Authors: Shaobin Fang, Xi Shi, Khor Waiho, Yueling Zhang, Huaiping Zheng, Yin Zhang, Hanafiah Fazhan, Huaqiang Tan, Mhd IkhwanuddinAbstract:In this study, we first identified male-specific SNP markers using restriction site-associated DNA sequencing, and further developed a PCR-based sex identification technique for Charybdis feriatus. A total of 296.96 million clean reads were obtained, with 114.95 and 182.01 million from females and males. After assembly and alignment, 10 SNP markers were identified being heterozygous in males but homozygous in females. Five markers were further confirmed to be male-specific in a large number of individuals. Moreover, two male-specific sense primers and a common antisense primer were designed, using which, a PCR-based genetic sex identification method was successfully developed and used to identify the sex of 103 individuals, with a result of 49 females and 54 males. The presence of male-specific SNP markers suggests an XX/XY sex determination System for C. feriatus. These findings should be helpful for better understanding sex determination mechanism, and drafting artificial breeding program in crustaceans.
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Table_2_High-Density Genetic Linkage Maps Provide Novel Insights Into ZW/ZZ Sex Determination System and Growth Performance in Mud Crab (Scylla paramamosain).xlsx
2019Co-Authors: Khor Waiho, Xi Shi, Yueling Zhang, Huaiping Zheng, Wenhua Liu, Shaobin Fang, Hanafiah Fazhan, Mhd IkhwanuddinAbstract:Mud crab, Scylla paramamosain is one of the most important crustacean species in global aquaculture. To determine the genetic basis of sex and growth-related traits in S. paramamosain, a high-density genetic linkage map with 16,701 single nucleotide polymorphisms (SNPs) was constructed using SLAF-seq and a full-sib family. The consensus map has 49 linkage groups, spanning 5,996.66 cM with an average marker-interval of 0.81 cM. A total of 516 SNP markers, including 8 female-specific SNPs segregated in two quantitative trait loci (QTLs) for phenotypic sex were located on LG32. The presence of female-specific SNP markers only on female linkage map, their segregation patterns and lower female: male recombination rate strongly suggest the conformation of a ZW/ZZ sex determination System in S. paramamosain. The QTLs of most (90%) growth-related traits were found within a small interval (25.18–33.74 cM) on LG46, highlighting the potential involvement of LG46 in growth. Four markers on LG46 were significantly associated with 10–16 growth-related traits. BW was only associated with marker 3846. Based on the annotation of transcriptome data, 11 and 2 candidate genes were identified within the QTL regions of sex and growth-related traits, respectively. The newly constructed high-density genetic linkage map with sex-specific SNPs, and the identified QTLs of sex- and growth-related traits serve as a valuable genetic resource and solid foundation for marker-assisted selection and genetic improvement of crustaceans.
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female specific snp markers provide insights into a wz zz sex determination System for mud crabs scylla paramamosain s tranquebarica and s serrata with a rapid method for genetic sex identification
BMC Genomics, 2018Co-Authors: Xi Shi, Mhd Ikhwanuddin, Khor Waiho, Guidong Miao, Fan Lin, Yueling Zhang, Huaiping Zheng, Wenhua Liu, Jude Juventus Aweya, Ghazali AzmieAbstract:Mud crabs, Scylla spp., are commercially important large-size marine crustaceans in the Indo-West Pacific region. As females have the higher growth rate and economic value, the production of all female stocks is extremely essential in aquaculture. However, the sex determination mechanism is still unclear. Development of sex-specific genetic markers based on next-generation sequencing proved to be an effective tool for discovering sex determination System in various animals. Restriction-site associated DNA sequencing (RAD-seq) was employed to isolate sex-specific SNP markers for S. paramamosain. A total of 335.6 million raw reads were obtained from 20 individuals, of which 204.7 million were from 10 females and 130.9 million from 10 males. After sequence assembly and female-male comparison, 20 SNP markers were identified to be sex-specific. Furthermore, ten SNPs in a short sequence (285 bp) were confirmed heterozygous in females and homozygous in males in a large population by PCR amplification and sequencing. Subsequently, a female-specific primer was successfully designed according to the female-specific nucleotide which could amplify an expected band from females but not from males. Thus, a rapid and effective method for molecular sexing in S. paramamosain was developed, meanwhile, this method could successfully identify the sex of S. tranquebarica and S. serrata. Finally, nine and four female-specific SNP markers were detected in S. tranquebarica and S. serrata, respectively. Sex-specific SNP markers were firstly identified in crab species and showed female heterogamety and male homogamety, which provided strong genetic evidence for a WZ/ZZ sex determination System in mud crabs S. paramamosain, S. tranquebarica and S. serrata. These findings will lay a solid foundation for the study of sex determination mechanism, sex chromosome evolution, and the development of mono-sex population in crustaceans.
Mara Cristina De Almeida - One of the best experts on this subject based on the ideXlab platform.
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population structure and genetic diversity analysis in gynaikothrips uzeli zimerman 1909 thysanoptera phlaeothripidae by rapd markers
Bulletin of Entomological Research, 2012Co-Authors: R O Brito, J C Silva, Roberto Ferreira Artoni, Marcelo Ricardo Vicari, Viviane Nogaroto, Rodrigo Rodrigues Matiello, Mara Cristina De AlmeidaAbstract:Thrips are small insects (0.5-3.0 mm) with distinct habits and life histories characterized by haplodiploid sex determination. In general, low levels of genetic diversity have been reported in haplodiploid insects, although most reports focus on the order Hymenoptera. Therefore, we used RAPD markers to evaluate the structure and both inter- and intra-population genetic variability of Gynaikothrips uzeli (Thysanoptera: Phlaeothripidae). Six populations, three from Parana state, southern Brazil, and three from Bahia, northeastern Brazil, were studied. Similarly to other haplodiploid insects, the genetic diversity of G. uzeli was reduced. This result is putatively related to the haplodiploid sex determination System, which yields little genetic variation, and to ecological traits of the studied species, such as the low dispersal abilities and life mode in leaf galls. All individuals were homogeneously clustered in their respective collection sites, forming two main groups in which populations from similar environments were more closely related. The analyzed populations were highly structured, and the genetic variation was higher among than within populations.
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evolutionary chromosomal differentiation among four species of conoderus eschscholtz 1829 coleoptera elateridae agrypninae conoderini detected by standard staining c banding silver nitrate impregnation and cma3 da dapi staining
Genetica, 2006Co-Authors: Marielle Cristina Schneider, Cleide Costa, Mara Cristina De Almeida, Simone Policena Rosa, Doralice Maria CellaAbstract:The speciose Brazilian Elateridae fauna is characterized by high karyotypic diversity, including one species (Chalcolepidius zonatus Eschscholtz, 1829) with the lowest diploid number within any Coleoptera order. Cytogenetic analysis of Conoderus dimidiatus Germar, 1839, C. scalaris (Germar, 1824,) C. ternarius Germar, 1839, and C. stigmosus Germar, 1839 by standard and differential staining was performed with the aim of establishing mechanisms of karyotypic differentiation in these species. Conoderus dimidiatus, C. scalaris, and C. ternarius have diploid numbers of 2n(♂) = 17 and 2n(♀) = 18, and a X0/XX sex determination System, similar to that encountered in the majority of Conoderini species. The karyotype of C. stigmosus was characterized by a diploid number of 2n=16 and a neoXY/neoXX sex determination System that was highly differentiated from other species of the genus. Some features of the mitotic and meiotic chromosomes suggest an autosome/ancestral X chromosome fusion as the cause of the neoXY System origin in C. stigmosus. C-banding and silver impregnation techniques showed that the four Conoderus species possess similar chromosomal characteristics to those registered in most Polyphaga species, including pericentromeric C band and autosomal NORs. Triple staining techniques including CMA3/DA/DAPI also provided useful information for differentiating these Conoderus species. These techniques revealed unique GC-rich heterochromatin associated with NORs in C. scalaris and C. stigmosus and CMA3-heteromorphism in C. scalaris and C. ternarius.
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evolutionary chromosomal differentiation among four species of conoderus eschscholtz 1829 coleoptera elateridae agrypninae conoderini detected by standard staining c banding silver nitrate impregnation and cma3 da dapi staining
Genetica, 2006Co-Authors: Marielle Cristina Schneider, Cleide Costa, Mara Cristina De Almeida, Simone Policena Rosa, Doralice Maria CellaAbstract:The speciose Brazilian Elateridae fauna is characterized by high karyotypic diversity, including one species (Chalcolepidius zonatus Eschscholtz, 1829) with the lowest diploid number within any Coleoptera order. Cytogenetic analysis of Conoderus dimidiatus Germar, 1839, C. scalaris (Germar, 1824,) C. ternarius Germar, 1839, and C. stigmosus Germar, 1839 by standard and differential staining was performed with the aim of establishing mechanisms of karyotypic differentiation in these species. Conoderus dimidiatus, C. scalaris, and C. ternarius have diploid numbers of 2n(male) = 17 and 2n(female) = 18, and a X0/XX sex determination System, similar to that encountered in the majority of Conoderini species. The karyotype of C. stigmosus was characterized by a diploid number of 2n = 16 and a neoXY/neoXX sex determination System that was highly differentiated from other species of the genus. Some features of the mitotic and meiotic chromosomes suggest an autosome/ancestral X chromosome fusion as the cause of the neoXY System origin in C. stigmosus. C-banding and silver impregnation techniques showed that the four Conoderus species possess similar chromosomal characteristics to those registered in most Polyphaga species, including pericentromeric C band and autosomal NORs. Triple staining techniques including CMA3/DA/DAPI also provided useful information for differentiating these Conoderus species. These techniques revealed unique GC-rich heterochromatin associated with NORs in C. scalaris and C. stigmosus and CMA3-heteromorphism in C. scalaris and C. ternarius.
Mariela Nieves - One of the best experts on this subject based on the ideXlab platform.
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meiotic characterization and sex determination System of neotropical primates bolivian squirrel monkey saimiri boliviensis primates cebidae
American Journal of Primatology, 2007Co-Authors: Eliana Ruth Steinberg, Mariela Nieves, Marta D. MudryAbstract:The chromosomal sex determination System differs among platyrrhine monkeys more than any other group of primates. Although a number of studies have investigated mitotic chromosomes across platyrrhine species, the meiotic chromosomes of many genera have not yet been described. The goal of this study was to characterize the sex determination System of Saimiri boliviensis. We described for the first time the meiotic cycle, confirming the sexual System in germ cells from testicular biopsies of four adult male S. boliviensis. All specimens were weighed and testicular volume was measured. We observed 22 bivalents corresponding to 2N = 44, and a "human-like" XY bivalent was found in diakinesis/metaphase I. In addition, mitotic studies from blood samples of both sexes were performed and G- and C-banding patterns agreed with previously reported karylogy of S. boliviensis boliviensis. Further meiotic studies should be performed in New World primates based on the great value of those studies for Systematic evolutionary biology and conservation programs.
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RESEARCH ARTICLE Meiotic Characterization and Sex Determination System of Neotropical Primates: Bolivian Squirrel Monkey Saimiri boliviensis (Primates: Cebidae)
2007Co-Authors: Eliana Ruth Steinberg, Mariela Nieves, Marta D. MudryAbstract:The chromosomal sex determination System differs among platyrrhine monkeys more than any other group of primates. Although a number of studies have investigated mitotic chromosomes across platyrrhine species, the meiotic chromosomes of many genera have not yet been described. The goal of this study was to characterize the sex determination System of Saimiri boliviensis. We described for the first time the meiotic cycle, confirming the sexual System in germ cells from testicular biopsies of four adult male S. boliviensis. All specimens were weighed and testicular volume was measured. We observed 22 bivalents corresponding to 2N 544, and a ‘‘human-like’’ XY bivalent was found in diakinesis/ metaphase I. In addition, mitotic studies from blood samples of both sexes were performed and G- and C-banding patterns agreed with previously reported karylogy of S. boliviensis boliviensis. Further meiotic studies should be performed in New World primates based on the great value of those studies for Systematic evolutionary biology and conservation