The Experts below are selected from a list of 69 Experts worldwide ranked by ideXlab platform

Robert Reenan - One of the best experts on this subject based on the ideXlab platform.

  • Control of human Potassium Channel inactivation by editing of a small mRNA hairpin
    Nature Structural & Molecular Biology, 2004
    Co-Authors: Tarun Bhalla, Joshua J C Rosenthal, Miguel Holmgren, Robert Reenan
    Abstract:

    Genomic recoding by A→I RNA editing plays an important role in diversifying the proteins involved in electrical excitability. Here, we describe editing of an intronless Potassium Channel gene. A small region of human K_V1.1 mRNA sequence directs efficient modification of one adenosine by human adenosine deaminase acting on RNA 2 (hADAR2). Mutational analysis shows that this region adopts a hairpin structure. Electrophysiological characterization reveals that the editing event (I/V) profoundly affects Channel inactivation conferred by accessory β subunits. Drosophila melanogaster Shaker Channels, mimicking this editing event through mutation, exhibit a similar effect. In addition, we demonstrate that mRNAs for the paralogous D. melanogaster Shab Potassium Channel are edited at the same position by fly ADAR—a clear example of convergent evolution driven by adenosine deamination. These results suggest an ancient and key regulatory role for this residue in K_V Channels.

Tarun Bhalla - One of the best experts on this subject based on the ideXlab platform.

  • Control of human Potassium Channel inactivation by editing of a small mRNA hairpin
    Nature Structural & Molecular Biology, 2004
    Co-Authors: Tarun Bhalla, Joshua J C Rosenthal, Miguel Holmgren, Robert Reenan
    Abstract:

    Genomic recoding by A→I RNA editing plays an important role in diversifying the proteins involved in electrical excitability. Here, we describe editing of an intronless Potassium Channel gene. A small region of human K_V1.1 mRNA sequence directs efficient modification of one adenosine by human adenosine deaminase acting on RNA 2 (hADAR2). Mutational analysis shows that this region adopts a hairpin structure. Electrophysiological characterization reveals that the editing event (I/V) profoundly affects Channel inactivation conferred by accessory β subunits. Drosophila melanogaster Shaker Channels, mimicking this editing event through mutation, exhibit a similar effect. In addition, we demonstrate that mRNAs for the paralogous D. melanogaster Shab Potassium Channel are edited at the same position by fly ADAR—a clear example of convergent evolution driven by adenosine deamination. These results suggest an ancient and key regulatory role for this residue in K_V Channels.

Joshua J C Rosenthal - One of the best experts on this subject based on the ideXlab platform.

  • Control of human Potassium Channel inactivation by editing of a small mRNA hairpin
    Nature Structural & Molecular Biology, 2004
    Co-Authors: Tarun Bhalla, Joshua J C Rosenthal, Miguel Holmgren, Robert Reenan
    Abstract:

    Genomic recoding by A→I RNA editing plays an important role in diversifying the proteins involved in electrical excitability. Here, we describe editing of an intronless Potassium Channel gene. A small region of human K_V1.1 mRNA sequence directs efficient modification of one adenosine by human adenosine deaminase acting on RNA 2 (hADAR2). Mutational analysis shows that this region adopts a hairpin structure. Electrophysiological characterization reveals that the editing event (I/V) profoundly affects Channel inactivation conferred by accessory β subunits. Drosophila melanogaster Shaker Channels, mimicking this editing event through mutation, exhibit a similar effect. In addition, we demonstrate that mRNAs for the paralogous D. melanogaster Shab Potassium Channel are edited at the same position by fly ADAR—a clear example of convergent evolution driven by adenosine deamination. These results suggest an ancient and key regulatory role for this residue in K_V Channels.

Miguel Holmgren - One of the best experts on this subject based on the ideXlab platform.

  • Control of human Potassium Channel inactivation by editing of a small mRNA hairpin
    Nature Structural & Molecular Biology, 2004
    Co-Authors: Tarun Bhalla, Joshua J C Rosenthal, Miguel Holmgren, Robert Reenan
    Abstract:

    Genomic recoding by A→I RNA editing plays an important role in diversifying the proteins involved in electrical excitability. Here, we describe editing of an intronless Potassium Channel gene. A small region of human K_V1.1 mRNA sequence directs efficient modification of one adenosine by human adenosine deaminase acting on RNA 2 (hADAR2). Mutational analysis shows that this region adopts a hairpin structure. Electrophysiological characterization reveals that the editing event (I/V) profoundly affects Channel inactivation conferred by accessory β subunits. Drosophila melanogaster Shaker Channels, mimicking this editing event through mutation, exhibit a similar effect. In addition, we demonstrate that mRNAs for the paralogous D. melanogaster Shab Potassium Channel are edited at the same position by fly ADAR—a clear example of convergent evolution driven by adenosine deamination. These results suggest an ancient and key regulatory role for this residue in K_V Channels.

Leon Islas - One of the best experts on this subject based on the ideXlab platform.