The Experts below are selected from a list of 3 Experts worldwide ranked by ideXlab platform
South Glamorgan - One of the best experts on this subject based on the ideXlab platform.
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Hypersensitivity pneumonitis in a technician using Pauli's reagent
2016Co-Authors: W V Evans, A Seaton, Ough Hospital, South GlamorganAbstract:ABSTRAcr A technician working in a medical laboratory used a spray of sodium diazobenzenesulphate (Pauli's reagent) in chromatography. She developed a respiratory illness with both airways obstruction and radiographic and physiological evidence of interstitial pneumonitis. An occupational type of challenge test was followed by both immediate and late bronchial obstructive responses, by a fall in arterial oxygen tension, and by increased radiographic Shadowing. Histology of a lung biopsy specimen, a low serum C3, and a positive skin prick test to the reagent suggested that the illness was a hypersensitivity reaction to Pauli's reagent. Pauli's reagent is the sodium salt of diazobenzene-sulphonic acid, which is produced by the reaction between cold sulphanilic acid, hydrochloric acid, and sodium nitrite. The sodium salt of the rela-tively unstable diazobenzenesulphonic acid is formed by adding sodium carbonate. The reagent is widely used in laboratories throughout the world for identifying aryl amines and phenols. This patient was using the reagent as a spray in chro-matography work. Case history A 33-year-old medical school laboratory technician first developed retrosternal discomfort in 1969. She was released from work for a week, prescribed an antibiotic, and her symptoms disappeared. In 1974 she developed further chest discomfort associated with a non-productive cough. Her symptoms gradually progressed, and by the summer of 1977 she had become breathless on moderate exertion. Eventually she related her cough and dyspnoea to the chemicals, and in particular the spray, used at her work. At this time she was referred for further investigation. Detailed inquiry into her work showed regular, twice weekly, use of Pauli's reagent (fig 1). She mixed the cold sulphanilic and hydrochloric acids with sodium nitrite on an open bench, then placed the mixture in a refrigerator for ten minutes before adding the sodium carbonate. The reagent was immediately sprayed on to chromatograph
W V Evans - One of the best experts on this subject based on the ideXlab platform.
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Hypersensitivity pneumonitis in a technician using Pauli's reagent
2016Co-Authors: W V Evans, A Seaton, Ough Hospital, South GlamorganAbstract:ABSTRAcr A technician working in a medical laboratory used a spray of sodium diazobenzenesulphate (Pauli's reagent) in chromatography. She developed a respiratory illness with both airways obstruction and radiographic and physiological evidence of interstitial pneumonitis. An occupational type of challenge test was followed by both immediate and late bronchial obstructive responses, by a fall in arterial oxygen tension, and by increased radiographic Shadowing. Histology of a lung biopsy specimen, a low serum C3, and a positive skin prick test to the reagent suggested that the illness was a hypersensitivity reaction to Pauli's reagent. Pauli's reagent is the sodium salt of diazobenzene-sulphonic acid, which is produced by the reaction between cold sulphanilic acid, hydrochloric acid, and sodium nitrite. The sodium salt of the rela-tively unstable diazobenzenesulphonic acid is formed by adding sodium carbonate. The reagent is widely used in laboratories throughout the world for identifying aryl amines and phenols. This patient was using the reagent as a spray in chro-matography work. Case history A 33-year-old medical school laboratory technician first developed retrosternal discomfort in 1969. She was released from work for a week, prescribed an antibiotic, and her symptoms disappeared. In 1974 she developed further chest discomfort associated with a non-productive cough. Her symptoms gradually progressed, and by the summer of 1977 she had become breathless on moderate exertion. Eventually she related her cough and dyspnoea to the chemicals, and in particular the spray, used at her work. At this time she was referred for further investigation. Detailed inquiry into her work showed regular, twice weekly, use of Pauli's reagent (fig 1). She mixed the cold sulphanilic and hydrochloric acids with sodium nitrite on an open bench, then placed the mixture in a refrigerator for ten minutes before adding the sodium carbonate. The reagent was immediately sprayed on to chromatograph
A Seaton - One of the best experts on this subject based on the ideXlab platform.
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Hypersensitivity pneumonitis in a technician using Pauli's reagent
2016Co-Authors: W V Evans, A Seaton, Ough Hospital, South GlamorganAbstract:ABSTRAcr A technician working in a medical laboratory used a spray of sodium diazobenzenesulphate (Pauli's reagent) in chromatography. She developed a respiratory illness with both airways obstruction and radiographic and physiological evidence of interstitial pneumonitis. An occupational type of challenge test was followed by both immediate and late bronchial obstructive responses, by a fall in arterial oxygen tension, and by increased radiographic Shadowing. Histology of a lung biopsy specimen, a low serum C3, and a positive skin prick test to the reagent suggested that the illness was a hypersensitivity reaction to Pauli's reagent. Pauli's reagent is the sodium salt of diazobenzene-sulphonic acid, which is produced by the reaction between cold sulphanilic acid, hydrochloric acid, and sodium nitrite. The sodium salt of the rela-tively unstable diazobenzenesulphonic acid is formed by adding sodium carbonate. The reagent is widely used in laboratories throughout the world for identifying aryl amines and phenols. This patient was using the reagent as a spray in chro-matography work. Case history A 33-year-old medical school laboratory technician first developed retrosternal discomfort in 1969. She was released from work for a week, prescribed an antibiotic, and her symptoms disappeared. In 1974 she developed further chest discomfort associated with a non-productive cough. Her symptoms gradually progressed, and by the summer of 1977 she had become breathless on moderate exertion. Eventually she related her cough and dyspnoea to the chemicals, and in particular the spray, used at her work. At this time she was referred for further investigation. Detailed inquiry into her work showed regular, twice weekly, use of Pauli's reagent (fig 1). She mixed the cold sulphanilic and hydrochloric acids with sodium nitrite on an open bench, then placed the mixture in a refrigerator for ten minutes before adding the sodium carbonate. The reagent was immediately sprayed on to chromatograph
Ough Hospital - One of the best experts on this subject based on the ideXlab platform.
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Hypersensitivity pneumonitis in a technician using Pauli's reagent
2016Co-Authors: W V Evans, A Seaton, Ough Hospital, South GlamorganAbstract:ABSTRAcr A technician working in a medical laboratory used a spray of sodium diazobenzenesulphate (Pauli's reagent) in chromatography. She developed a respiratory illness with both airways obstruction and radiographic and physiological evidence of interstitial pneumonitis. An occupational type of challenge test was followed by both immediate and late bronchial obstructive responses, by a fall in arterial oxygen tension, and by increased radiographic Shadowing. Histology of a lung biopsy specimen, a low serum C3, and a positive skin prick test to the reagent suggested that the illness was a hypersensitivity reaction to Pauli's reagent. Pauli's reagent is the sodium salt of diazobenzene-sulphonic acid, which is produced by the reaction between cold sulphanilic acid, hydrochloric acid, and sodium nitrite. The sodium salt of the rela-tively unstable diazobenzenesulphonic acid is formed by adding sodium carbonate. The reagent is widely used in laboratories throughout the world for identifying aryl amines and phenols. This patient was using the reagent as a spray in chro-matography work. Case history A 33-year-old medical school laboratory technician first developed retrosternal discomfort in 1969. She was released from work for a week, prescribed an antibiotic, and her symptoms disappeared. In 1974 she developed further chest discomfort associated with a non-productive cough. Her symptoms gradually progressed, and by the summer of 1977 she had become breathless on moderate exertion. Eventually she related her cough and dyspnoea to the chemicals, and in particular the spray, used at her work. At this time she was referred for further investigation. Detailed inquiry into her work showed regular, twice weekly, use of Pauli's reagent (fig 1). She mixed the cold sulphanilic and hydrochloric acids with sodium nitrite on an open bench, then placed the mixture in a refrigerator for ten minutes before adding the sodium carbonate. The reagent was immediately sprayed on to chromatograph