The Experts below are selected from a list of 39561 Experts worldwide ranked by ideXlab platform
Daniel G. Baden - One of the best experts on this subject based on the ideXlab platform.
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A competitive ELISA to detect brevetoxins from Karenia brevis (formerly Gymnodinium breve) in seawater, Shellfish, and mammalian body fluid
Environmental Health Perspectives, 2002Co-Authors: Jérôme Naar, Andrea Bourdelais, Philip L. Whitney, Carmelo R Tomas, Julia Kubanek, Karen Steidinger, Johnny Lancaster, Daniel G. BadenAbstract:We developed a competitive enzyme-linked immunosorbent assay (ELISA) to analyze brevetoxins, using goat anti-brevetoxin antibodies obtained after immunization with keyhole limpet hemocyanin-brevetoxin conjugates, in combination with a three-step signal amplification process. The procedure, which used secondary biotinylated antibodies, streptavidine-horseradish peroxidase conjugate, and chromogenic enzyme substrate, was useful in reducing nonspecific background signals commonly observed with complex matrices. This competitive ELISA detected brevetoxins in seawater, Shellfish extract and homogenate, and mammalian body fluid such as urine and serum without pretreatment, dilution, or purification. We investigated the application of this technique for Shellfish monitoring by spiking Shellfish meat with brevetoxins and by analyzing oysters from two commercial Shellfish beds in Florida that were exposed to a bloom of Karenia brevis (formerly Gymnodinium breve). We performed brevetoxin analysis of Shellfish extracts and homogenates by ELISA and compared it with the mouse bioassay and receptor binding assay. The detection limit for brevetoxins in spiked oysters was 2.5 µg/100 g Shellfish meat. This assay appears to be a useful tool for neurotoxic Shellfish poisoning monitoring in Shellfish and seawater,
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A competitive ELISA to detect brevetoxins from Karenia brevis (formerly Gymnodinium breve) in seawater, shelfish, and mamalian body fluid
Environmental Health Perspectives, 2002Co-Authors: Jérôme Naar, Andrea Bourdelais, Carmelo Tomas, Philip L. Whitney, Leanne Flewelling, Johnny Lancaster Karen Steidinger, Julia Kubanek, Daniel G. BadenAbstract:We developed a competitive enzyme-linked immunosorbent assay (ELISA) to analyze brevetoxins, using goat anti-brevetoxin antibodies obtained after immunization with keyhole limpet hemocyanin-brevetoxin conjugates, in combination with a three-step signal amplification process. The procedure, which used secondary biotinylated antibodies, streptavidine-horseradish peroxidase conjugate, and chromogenic enzyme substrate, was useful in reducing nonspecific background signals commonly observed with complex matrices. This competitive ELISA detected brevetoxins in seawater, Shellfish extract and homogenate, and mammalian body fluid such as urine and serum without pretreatment, dilution, or purification. We investigated the application of this technique for Shellfish monitoring by spiking Shellfish meat with brevetoxins and by analyzing oysters from two commercial Shellfish beds in Florida that were exposed to a bloom of Karenia brevis (formerly Gymnodinium breve). We performed brevetoxin analysis of Shellfish extracts and homogenates by ELISA and compared it with the mouse bioassay and receptor binding assay. The detection limit for brevetoxins in spiked oysters was 2.5 μg/100 g Shellfish meat. This assay appears to be a useful tool for neurotoxic Shellfish poisoning monitoring in Shellfish and seawater, and for mammalian exposure diagnostics, and significantly reduces the time required for analyses.
Jérôme Naar - One of the best experts on this subject based on the ideXlab platform.
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A competitive ELISA to detect brevetoxins from Karenia brevis (formerly Gymnodinium breve) in seawater, Shellfish, and mammalian body fluid
Environmental Health Perspectives, 2002Co-Authors: Jérôme Naar, Andrea Bourdelais, Philip L. Whitney, Carmelo R Tomas, Julia Kubanek, Karen Steidinger, Johnny Lancaster, Daniel G. BadenAbstract:We developed a competitive enzyme-linked immunosorbent assay (ELISA) to analyze brevetoxins, using goat anti-brevetoxin antibodies obtained after immunization with keyhole limpet hemocyanin-brevetoxin conjugates, in combination with a three-step signal amplification process. The procedure, which used secondary biotinylated antibodies, streptavidine-horseradish peroxidase conjugate, and chromogenic enzyme substrate, was useful in reducing nonspecific background signals commonly observed with complex matrices. This competitive ELISA detected brevetoxins in seawater, Shellfish extract and homogenate, and mammalian body fluid such as urine and serum without pretreatment, dilution, or purification. We investigated the application of this technique for Shellfish monitoring by spiking Shellfish meat with brevetoxins and by analyzing oysters from two commercial Shellfish beds in Florida that were exposed to a bloom of Karenia brevis (formerly Gymnodinium breve). We performed brevetoxin analysis of Shellfish extracts and homogenates by ELISA and compared it with the mouse bioassay and receptor binding assay. The detection limit for brevetoxins in spiked oysters was 2.5 µg/100 g Shellfish meat. This assay appears to be a useful tool for neurotoxic Shellfish poisoning monitoring in Shellfish and seawater,
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A competitive ELISA to detect brevetoxins from Karenia brevis (formerly Gymnodinium breve) in seawater, shelfish, and mamalian body fluid
Environmental Health Perspectives, 2002Co-Authors: Jérôme Naar, Andrea Bourdelais, Carmelo Tomas, Philip L. Whitney, Leanne Flewelling, Johnny Lancaster Karen Steidinger, Julia Kubanek, Daniel G. BadenAbstract:We developed a competitive enzyme-linked immunosorbent assay (ELISA) to analyze brevetoxins, using goat anti-brevetoxin antibodies obtained after immunization with keyhole limpet hemocyanin-brevetoxin conjugates, in combination with a three-step signal amplification process. The procedure, which used secondary biotinylated antibodies, streptavidine-horseradish peroxidase conjugate, and chromogenic enzyme substrate, was useful in reducing nonspecific background signals commonly observed with complex matrices. This competitive ELISA detected brevetoxins in seawater, Shellfish extract and homogenate, and mammalian body fluid such as urine and serum without pretreatment, dilution, or purification. We investigated the application of this technique for Shellfish monitoring by spiking Shellfish meat with brevetoxins and by analyzing oysters from two commercial Shellfish beds in Florida that were exposed to a bloom of Karenia brevis (formerly Gymnodinium breve). We performed brevetoxin analysis of Shellfish extracts and homogenates by ELISA and compared it with the mouse bioassay and receptor binding assay. The detection limit for brevetoxins in spiked oysters was 2.5 μg/100 g Shellfish meat. This assay appears to be a useful tool for neurotoxic Shellfish poisoning monitoring in Shellfish and seawater, and for mammalian exposure diagnostics, and significantly reduces the time required for analyses.
Bee Wah Lee - One of the best experts on this subject based on the ideXlab platform.
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Shellfish and house dust mite allergies is the link tropomyosin
Allergy Asthma and Immunology Research, 2016Co-Authors: Lydia Helena Wong, Bee Wah Lee, Chiunghui HuangAbstract:Crustacean Shellfish allergy is an important cause of food allergy and anaphylaxis in Asia. The major allergen in Shellfish allergy is tropomyosin, a pan-allergen that is also found in house dust mites and cockroaches. Tropomyosins from house dust mites (HDMs) have a high sequence homology to Shellfish tropomyosins, and cross-reactivity between HDM and shrimp tropomyosins has been demonstrated. Exposure to inhaled tropomyosins from house dust mites has been postulated to be the primary sensitizer for Shellfish allergy, in a reaction analogous to the oral allergy (inhalant-food) syndrome. This notion is supported by indirect data from the effects of HDM immunotherapy on Shellfish allergy, and strong correlations of Shellfish and HDM sensitization. HDM immunotherapy has been reported to induce both shrimp allergy in non-allergic patients and shrimp tolerance in shrimp-allergic patients. Epidemiological surveys have also demonstrated a strong correlation between Shellfish and HDM sensitization in both hospital-based and community-based studies. Unexposed populations have also been shown to develop sensitization-Shellfish sensitization in orthodox Jews with no history of Shellfish consumption was associated with HDM sensitization. Reciprocally, HDM sensitization in an Icelandic population living in a HDM-free environment was associated with shrimp sensitization. In vitro IgE inhibition studies on sera in shrimp-allergic Spanish patients indicate that mites are the primary sensitizer in shrimp-allergic patients living in humid and warm climates. Current data supports the hypothesis that tropomyosin is the link between HDM and Shellfish allergies. The role of tropomyosin in HDM and Shellfish allergies is a fertile field for investigation as it may provide novel immunotherapeutic strategies for Shellfish allergy.
Andrea Bourdelais - One of the best experts on this subject based on the ideXlab platform.
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A competitive ELISA to detect brevetoxins from Karenia brevis (formerly Gymnodinium breve) in seawater, Shellfish, and mammalian body fluid
Environmental Health Perspectives, 2002Co-Authors: Jérôme Naar, Andrea Bourdelais, Philip L. Whitney, Carmelo R Tomas, Julia Kubanek, Karen Steidinger, Johnny Lancaster, Daniel G. BadenAbstract:We developed a competitive enzyme-linked immunosorbent assay (ELISA) to analyze brevetoxins, using goat anti-brevetoxin antibodies obtained after immunization with keyhole limpet hemocyanin-brevetoxin conjugates, in combination with a three-step signal amplification process. The procedure, which used secondary biotinylated antibodies, streptavidine-horseradish peroxidase conjugate, and chromogenic enzyme substrate, was useful in reducing nonspecific background signals commonly observed with complex matrices. This competitive ELISA detected brevetoxins in seawater, Shellfish extract and homogenate, and mammalian body fluid such as urine and serum without pretreatment, dilution, or purification. We investigated the application of this technique for Shellfish monitoring by spiking Shellfish meat with brevetoxins and by analyzing oysters from two commercial Shellfish beds in Florida that were exposed to a bloom of Karenia brevis (formerly Gymnodinium breve). We performed brevetoxin analysis of Shellfish extracts and homogenates by ELISA and compared it with the mouse bioassay and receptor binding assay. The detection limit for brevetoxins in spiked oysters was 2.5 µg/100 g Shellfish meat. This assay appears to be a useful tool for neurotoxic Shellfish poisoning monitoring in Shellfish and seawater,
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A competitive ELISA to detect brevetoxins from Karenia brevis (formerly Gymnodinium breve) in seawater, shelfish, and mamalian body fluid
Environmental Health Perspectives, 2002Co-Authors: Jérôme Naar, Andrea Bourdelais, Carmelo Tomas, Philip L. Whitney, Leanne Flewelling, Johnny Lancaster Karen Steidinger, Julia Kubanek, Daniel G. BadenAbstract:We developed a competitive enzyme-linked immunosorbent assay (ELISA) to analyze brevetoxins, using goat anti-brevetoxin antibodies obtained after immunization with keyhole limpet hemocyanin-brevetoxin conjugates, in combination with a three-step signal amplification process. The procedure, which used secondary biotinylated antibodies, streptavidine-horseradish peroxidase conjugate, and chromogenic enzyme substrate, was useful in reducing nonspecific background signals commonly observed with complex matrices. This competitive ELISA detected brevetoxins in seawater, Shellfish extract and homogenate, and mammalian body fluid such as urine and serum without pretreatment, dilution, or purification. We investigated the application of this technique for Shellfish monitoring by spiking Shellfish meat with brevetoxins and by analyzing oysters from two commercial Shellfish beds in Florida that were exposed to a bloom of Karenia brevis (formerly Gymnodinium breve). We performed brevetoxin analysis of Shellfish extracts and homogenates by ELISA and compared it with the mouse bioassay and receptor binding assay. The detection limit for brevetoxins in spiked oysters was 2.5 μg/100 g Shellfish meat. This assay appears to be a useful tool for neurotoxic Shellfish poisoning monitoring in Shellfish and seawater, and for mammalian exposure diagnostics, and significantly reduces the time required for analyses.
Julia Kubanek - One of the best experts on this subject based on the ideXlab platform.
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A competitive ELISA to detect brevetoxins from Karenia brevis (formerly Gymnodinium breve) in seawater, Shellfish, and mammalian body fluid
Environmental Health Perspectives, 2002Co-Authors: Jérôme Naar, Andrea Bourdelais, Philip L. Whitney, Carmelo R Tomas, Julia Kubanek, Karen Steidinger, Johnny Lancaster, Daniel G. BadenAbstract:We developed a competitive enzyme-linked immunosorbent assay (ELISA) to analyze brevetoxins, using goat anti-brevetoxin antibodies obtained after immunization with keyhole limpet hemocyanin-brevetoxin conjugates, in combination with a three-step signal amplification process. The procedure, which used secondary biotinylated antibodies, streptavidine-horseradish peroxidase conjugate, and chromogenic enzyme substrate, was useful in reducing nonspecific background signals commonly observed with complex matrices. This competitive ELISA detected brevetoxins in seawater, Shellfish extract and homogenate, and mammalian body fluid such as urine and serum without pretreatment, dilution, or purification. We investigated the application of this technique for Shellfish monitoring by spiking Shellfish meat with brevetoxins and by analyzing oysters from two commercial Shellfish beds in Florida that were exposed to a bloom of Karenia brevis (formerly Gymnodinium breve). We performed brevetoxin analysis of Shellfish extracts and homogenates by ELISA and compared it with the mouse bioassay and receptor binding assay. The detection limit for brevetoxins in spiked oysters was 2.5 µg/100 g Shellfish meat. This assay appears to be a useful tool for neurotoxic Shellfish poisoning monitoring in Shellfish and seawater,
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A competitive ELISA to detect brevetoxins from Karenia brevis (formerly Gymnodinium breve) in seawater, shelfish, and mamalian body fluid
Environmental Health Perspectives, 2002Co-Authors: Jérôme Naar, Andrea Bourdelais, Carmelo Tomas, Philip L. Whitney, Leanne Flewelling, Johnny Lancaster Karen Steidinger, Julia Kubanek, Daniel G. BadenAbstract:We developed a competitive enzyme-linked immunosorbent assay (ELISA) to analyze brevetoxins, using goat anti-brevetoxin antibodies obtained after immunization with keyhole limpet hemocyanin-brevetoxin conjugates, in combination with a three-step signal amplification process. The procedure, which used secondary biotinylated antibodies, streptavidine-horseradish peroxidase conjugate, and chromogenic enzyme substrate, was useful in reducing nonspecific background signals commonly observed with complex matrices. This competitive ELISA detected brevetoxins in seawater, Shellfish extract and homogenate, and mammalian body fluid such as urine and serum without pretreatment, dilution, or purification. We investigated the application of this technique for Shellfish monitoring by spiking Shellfish meat with brevetoxins and by analyzing oysters from two commercial Shellfish beds in Florida that were exposed to a bloom of Karenia brevis (formerly Gymnodinium breve). We performed brevetoxin analysis of Shellfish extracts and homogenates by ELISA and compared it with the mouse bioassay and receptor binding assay. The detection limit for brevetoxins in spiked oysters was 2.5 μg/100 g Shellfish meat. This assay appears to be a useful tool for neurotoxic Shellfish poisoning monitoring in Shellfish and seawater, and for mammalian exposure diagnostics, and significantly reduces the time required for analyses.
