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Christina Kruuse - One of the best experts on this subject based on the ideXlab platform.
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pde9a pde10a and pde11a expression in rat trigeminovascular pain Signalling System
Brain Research, 2009Co-Authors: Lars S Kruse, Jes Olesen, Steen Gammeltoft, Morten Moller, Maiken Tibaek, Christina KruuseAbstract:Abstract Activation of the trigeminovascular pain Signalling System, including cerebral arteries, meninges, trigeminal ganglion, and brain stem, is involved in migraine. Furthermore, stimulation of cyclic nucleotide (cAMP and cGMP) production as well as inhibition of phosphodiesterases (PDEs) induces headache and migraine. In order to investigate the possible role of PDE in the pain pathway of migraine, expression of the most recently discovered PDE subtypes (9A, 10A and 11A) in cerebral arteries, dura mater, and trigeminal ganglion and nucleus was examined. The presence of mRNA and protein in the middle cerebral artery, basilar artery, meninges, trigeminal ganglion, and spinal trigeminal nucleus of male Sprague–Dawley rats were investigated using real-time PCR, Western blot, and immunohistochemistry. The results were compared to two peripheral arteries: aorta and mesenteric artery, as well as neocortex and cerebellar cortex. Real-time PCR and Western blotting showed that PDE9A, PDE10A and PDE11A are expressed in components of the rat trigeminovascular pain Signalling System including middle cerebral artery, basilar artery, meninges, trigeminal ganglion and spinal trigeminal nucleus. Aorta and mesenteric artery as well as cerebral neocortex and cerebellar cortex also showed expression of PDE9A, PDE10A and PDE11A. Immunohistochemistry revealed that PDE9A, PDE10A and PDE11A are localised in the cytosol of nerve cell bodies of the trigeminal ganglion. We here present, for the first time, the expression of PDE9A, PDE10A, and PDE11A in the trigeminovascular System. The functional implications are yet unknown, but their localisation indicates that they may have a role in the pain pathway of migraine as well as trigeminal neuralgia and trigeminal autonomic cephalalgias.
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pde9a pde10a and pde11a expression in rat trigeminovascular pain Signalling System role in pathogenesis of migraine
BMC Pharmacology, 2007Co-Authors: Lars S Kruse, Jes Olesen, Steen Gammeltoft, Morten Moller, Maiken Tibaek, Christina KruuseAbstract:Background Activation of the trigeminovascular pain Signalling System, which includes cerebral arteries, meninges, trigeminal ganglion, and brain stem, may be involved in migraine. Furthermore, stimulation of cyclic nucleotide (cAMP and cGMP) production as well as inhibition of phosphodiesterases (PDEs) induces headache and migraine [1-4]. The aim is to study the expression of the most recently discovered PDE subtypes (9A, 10A and 11A) in cerebral arteries, the dura mater, and the trigeminal ganglion and nucleus. This may give a clue to a role in pathogenesis of migraine.
Pascal Favrel - One of the best experts on this subject based on the ideXlab platform.
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emergence of a cholecystokinin sulfakinin Signalling System in lophotrochozoa
Scientific Reports, 2018Co-Authors: Julie Schwartz, Marie Pierre Dubos, Jeremy Pasquier, Celine Zatylnygaudin, Pascal FavrelAbstract:Chordate gastrin/cholecystokinin (G/CCK) and ecdysozoan sulfakinin (SK) Signalling Systems represent divergent evolutionary scenarios of a common ancestral Signalling System. The present article investigates for the first time the evolution of the CCK/SK Signalling System in a member of the Lophotrochozoa, the second clade of protostome animals. We identified two G protein-coupled receptors (GPCR) in the oyster Crassostrea gigas (Mollusca), phylogenetically related to chordate CCK receptors (CCKR) and to ecdysozoan sulfakinin receptors (SKR). These receptors, Cragi-CCKR1 and Cragi-CCKR2, were characterised functionally using a cell-based assay. We identified di- and mono-sulphated forms of oyster Cragi-CCK1 (pEGAWDY(SO3H)DY(SO3H)GLGGGRF-NH2) as the potent endogenous agonists for these receptors. The Cragi-CCK genes were expressed in the visceral ganglia of the nervous System. The Cragi-CCKR1 gene was expressed in a variety of tissues, while Cragi-CCKR2 gene expression was more restricted to nervous tissues. An in vitro bioassay revealed that different forms of Cragi-CCK1 decreased the frequency of the spontaneous contractions of oyster hindgut. Expression analyses in oysters with contrasted nutritional statuses or in the course of their reproductive cycle highlighted the plausible role of Cragi-CCK Signalling in the regulation of feeding and its possible involvement in the coordination of nutrition and energy storage in the gonad. This study confirms the early origin of the CCK/SK Signalling System from the common bilaterian ancestor and delivers new insights into its structural and functional evolution in the lophotrochozoan lineage.
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Emergence of a cholecystokinin/sulfakinin Signalling System in Lophotrochozoa
Scientific Reports, 2018Co-Authors: Julie Schwartz, Marie Pierre Dubos, Jeremy Pasquier, Céline Zatylny-gaudin, Pascal FavrelAbstract:Chordate gastrin/cholecystokinin (G/CCK) and ecdysozoan sulfakinin (SK) Signalling Systems represent divergent evolutionary scenarios of a common ancestral Signalling System. The present article investigates for the first time the evolution of the CCK/SK Signalling System in a member of the Lophotrochozoa, the second clade of protostome animals. We identified two G protein-coupled receptors (GPCR) in the oyster Crassostrea gigas (Mollusca), phylogenetically related to chordate CCK receptors (CCKR) and to ecdysozoan sulfakinin receptors (SKR). These receptors, Cragi-CCKR1 and Cragi-CCKR2, were characterised functionally using a cell-based assay. We identified di- and mono-sulphated forms of oyster Cragi-CCK1 (pEGAWDY(SO3H)DY(SO3H)GLGGGRF-NH2) as the potent endogenous agonists for these receptors. The Cragi-CCK genes were expressed in the visceral ganglia of the nervous System. The Cragi-CCKR1 gene was expressed in a variety of tissues, while Cragi-CCKR2 gene expression was more restricted to nervous tissues. An in vitro bioassay revealed that different forms of Cragi-CCK1 decreased the frequency of the spontaneous contractions of oyster hindgut. Expression analyses in oysters with contrasted nutritional statuses or in the course of their reproductive cycle highlighted the plausible role of Cragi-CCK Signalling in the regulation of feeding and its possible involvement in the coordination of nutrition and energy storage in the gonad. This study confirms the early origin of the CCK/SK Signalling System from the common bilaterian ancestor and delivers new insights into its structural and functional evolution in the lophotrochozoan lineage.
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Characterization of a tachykinin Signalling System in the bivalve mollusc Crassostrea gigas
General and Comparative Endocrinology, 2018Co-Authors: Marie Pierre Dubos, Julie Schwartz, Jeremy Pasquier, Sven Zels, Liliane Schoofs, Pascal FavrelAbstract:Although tachykinin-like neuropeptides have been identified in molluscs more than two decades ago, knowledge on their function and Signalling has so far remained largely elusive. We developed a cell-based assay to address the functionality of the tachykinin G-protein coupled receptor (Cragi-TKR) in the oyster Crassostrea gigas. The oyster tachykinin neuropeptides that are derived from the tachykinin precursor gene Cragi-TK activate the Cragi-TKR in nanomolar concentrations. Receptor activation is sensitive to Ala-substitution of critical Cragi-TK amino acid residues. The Cragi-TKR gene is expressed in a variety of tissues, albeit at higher levels in the visceral ganglia (VG) of the nervous System. Fluctuations of Cragi-TKR expression is in line with a role for TK Signalling in C. gigas reproduction. The expression level of the Cragi-TK gene in the VG depends on the nutritional status of the oyster, suggesting a role for TK Signalling in the complex regulation of feeding in C. gigas.
Tobias Meyer - One of the best experts on this subject based on the ideXlab platform.
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a polarized ca2 diacylglycerol and stim1 Signalling System regulates directed cell migration
Nature Cell Biology, 2014Co-Authors: Fengchiao Tsai, Hee Won Yang, Seth Malmersjo, Arnold Hayer, Silvia Carrasco, Akiko Seki, Tobias MeyerAbstract:Meyer and colleagues used live-cell imaging to study the spatial organization of Ca2+ Signalling network components during cell migration.
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a polarized ca2 diacylglycerol and stim1 Signalling System regulates directed cell migration
Nature Cell Biology, 2014Co-Authors: Fengchiao Tsai, Hee Won Yang, Seth Malmersjo, Arnold Hayer, Silvia Carrasco, Akiko Seki, Tobias MeyerAbstract:Ca(2+) signals control cell migration by regulating forward movement and cell adhesion. However, it is not well understood how Ca(2+)-regulatory proteins and second messengers are spatially organized in migrating cells. Here we show that receptor tyrosine kinase and phospholipase C Signalling are restricted to the front of migrating endothelial leader cells, triggering local Ca(2+) pulses, local depletion of Ca(2+) in the endoplasmic reticulum and local activation of STIM1, supporting pulsatile front retraction and adhesion. At the same time, the mediator of store-operated Ca(2+) influx, STIM1, is transported by microtubule plus ends to the front. Furthermore, higher Ca(2+) pump rates in the front relative to the back of the plasma membrane enable effective local Ca(2+) Signalling by locally decreasing basal Ca(2+). Finally, polarized phospholipase C Signalling generates a diacylglycerol gradient towards the front that promotes persistent forward migration. Thus, cells employ an integrated Ca(2+) control System with polarized Ca(2+) Signalling proteins and second messengers to synergistically promote directed cell migration.
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A polarized Ca^2+, diacylglycerol and STIM1 Signalling System regulates directed cell migration
Nature Cell Biology, 2014Co-Authors: Fengchiao Tsai, Hee Won Yang, Seth Malmersjo, Arnold Hayer, Silvia Carrasco, Akiko Seki, Tobias MeyerAbstract:Meyer and colleagues used live-cell imaging to study the spatial organization of Ca^2+ Signalling network components during cell migration. Ca^2+ signals control cell migration by regulating forward movement and cell adhesion. However, it is not well understood how Ca^2+-regulatory proteins and second messengers are spatially organized in migrating cells. Here we show that receptor tyrosine kinase and phospholipase C Signalling are restricted to the front of migrating endothelial leader cells, triggering local Ca^2+ pulses, local depletion of Ca^2+ in the endoplasmic reticulum and local activation of STIM1, supporting pulsatile front retraction and adhesion. At the same time, the mediator of store-operated Ca^2+ influx, STIM1, is transported by microtubule plus ends to the front. Furthermore, higher Ca^2+ pump rates in the front relative to the back of the plasma membrane enable effective local Ca^2+ Signalling by locally decreasing basal Ca^2+. Finally, polarized phospholipase C Signalling generates a diacylglycerol gradient towards the front that promotes persistent forward migration. Thus, cells employ an integrated Ca^2+ control System with polarized Ca^2+ Signalling proteins and second messengers to synergistically promote directed cell migration.
Lars S Kruse - One of the best experts on this subject based on the ideXlab platform.
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pde9a pde10a and pde11a expression in rat trigeminovascular pain Signalling System
Brain Research, 2009Co-Authors: Lars S Kruse, Jes Olesen, Steen Gammeltoft, Morten Moller, Maiken Tibaek, Christina KruuseAbstract:Abstract Activation of the trigeminovascular pain Signalling System, including cerebral arteries, meninges, trigeminal ganglion, and brain stem, is involved in migraine. Furthermore, stimulation of cyclic nucleotide (cAMP and cGMP) production as well as inhibition of phosphodiesterases (PDEs) induces headache and migraine. In order to investigate the possible role of PDE in the pain pathway of migraine, expression of the most recently discovered PDE subtypes (9A, 10A and 11A) in cerebral arteries, dura mater, and trigeminal ganglion and nucleus was examined. The presence of mRNA and protein in the middle cerebral artery, basilar artery, meninges, trigeminal ganglion, and spinal trigeminal nucleus of male Sprague–Dawley rats were investigated using real-time PCR, Western blot, and immunohistochemistry. The results were compared to two peripheral arteries: aorta and mesenteric artery, as well as neocortex and cerebellar cortex. Real-time PCR and Western blotting showed that PDE9A, PDE10A and PDE11A are expressed in components of the rat trigeminovascular pain Signalling System including middle cerebral artery, basilar artery, meninges, trigeminal ganglion and spinal trigeminal nucleus. Aorta and mesenteric artery as well as cerebral neocortex and cerebellar cortex also showed expression of PDE9A, PDE10A and PDE11A. Immunohistochemistry revealed that PDE9A, PDE10A and PDE11A are localised in the cytosol of nerve cell bodies of the trigeminal ganglion. We here present, for the first time, the expression of PDE9A, PDE10A, and PDE11A in the trigeminovascular System. The functional implications are yet unknown, but their localisation indicates that they may have a role in the pain pathway of migraine as well as trigeminal neuralgia and trigeminal autonomic cephalalgias.
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pde9a pde10a and pde11a expression in rat trigeminovascular pain Signalling System role in pathogenesis of migraine
BMC Pharmacology, 2007Co-Authors: Lars S Kruse, Jes Olesen, Steen Gammeltoft, Morten Moller, Maiken Tibaek, Christina KruuseAbstract:Background Activation of the trigeminovascular pain Signalling System, which includes cerebral arteries, meninges, trigeminal ganglion, and brain stem, may be involved in migraine. Furthermore, stimulation of cyclic nucleotide (cAMP and cGMP) production as well as inhibition of phosphodiesterases (PDEs) induces headache and migraine [1-4]. The aim is to study the expression of the most recently discovered PDE subtypes (9A, 10A and 11A) in cerebral arteries, the dura mater, and the trigeminal ganglion and nucleus. This may give a clue to a role in pathogenesis of migraine.
Fengchiao Tsai - One of the best experts on this subject based on the ideXlab platform.
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a polarized ca2 diacylglycerol and stim1 Signalling System regulates directed cell migration
Nature Cell Biology, 2014Co-Authors: Fengchiao Tsai, Hee Won Yang, Seth Malmersjo, Arnold Hayer, Silvia Carrasco, Akiko Seki, Tobias MeyerAbstract:Meyer and colleagues used live-cell imaging to study the spatial organization of Ca2+ Signalling network components during cell migration.
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a polarized ca2 diacylglycerol and stim1 Signalling System regulates directed cell migration
Nature Cell Biology, 2014Co-Authors: Fengchiao Tsai, Hee Won Yang, Seth Malmersjo, Arnold Hayer, Silvia Carrasco, Akiko Seki, Tobias MeyerAbstract:Ca(2+) signals control cell migration by regulating forward movement and cell adhesion. However, it is not well understood how Ca(2+)-regulatory proteins and second messengers are spatially organized in migrating cells. Here we show that receptor tyrosine kinase and phospholipase C Signalling are restricted to the front of migrating endothelial leader cells, triggering local Ca(2+) pulses, local depletion of Ca(2+) in the endoplasmic reticulum and local activation of STIM1, supporting pulsatile front retraction and adhesion. At the same time, the mediator of store-operated Ca(2+) influx, STIM1, is transported by microtubule plus ends to the front. Furthermore, higher Ca(2+) pump rates in the front relative to the back of the plasma membrane enable effective local Ca(2+) Signalling by locally decreasing basal Ca(2+). Finally, polarized phospholipase C Signalling generates a diacylglycerol gradient towards the front that promotes persistent forward migration. Thus, cells employ an integrated Ca(2+) control System with polarized Ca(2+) Signalling proteins and second messengers to synergistically promote directed cell migration.
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A polarized Ca^2+, diacylglycerol and STIM1 Signalling System regulates directed cell migration
Nature Cell Biology, 2014Co-Authors: Fengchiao Tsai, Hee Won Yang, Seth Malmersjo, Arnold Hayer, Silvia Carrasco, Akiko Seki, Tobias MeyerAbstract:Meyer and colleagues used live-cell imaging to study the spatial organization of Ca^2+ Signalling network components during cell migration. Ca^2+ signals control cell migration by regulating forward movement and cell adhesion. However, it is not well understood how Ca^2+-regulatory proteins and second messengers are spatially organized in migrating cells. Here we show that receptor tyrosine kinase and phospholipase C Signalling are restricted to the front of migrating endothelial leader cells, triggering local Ca^2+ pulses, local depletion of Ca^2+ in the endoplasmic reticulum and local activation of STIM1, supporting pulsatile front retraction and adhesion. At the same time, the mediator of store-operated Ca^2+ influx, STIM1, is transported by microtubule plus ends to the front. Furthermore, higher Ca^2+ pump rates in the front relative to the back of the plasma membrane enable effective local Ca^2+ Signalling by locally decreasing basal Ca^2+. Finally, polarized phospholipase C Signalling generates a diacylglycerol gradient towards the front that promotes persistent forward migration. Thus, cells employ an integrated Ca^2+ control System with polarized Ca^2+ Signalling proteins and second messengers to synergistically promote directed cell migration.
