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Wolfram F Neiss - One of the best experts on this subject based on the ideXlab platform.

  • superior muscle reinnervation after autologous nerve graft or poly l lactide epsilon caprolactone plc Tube implantation in comparison to Silicone Tube repair
    Journal of Neuroscience Research, 2001
    Co-Authors: Antoni Valerocabre, Konstantin Tsironis, Emmanouil Skouras, Gabriele Perego, Xavier Navarro, Wolfram F Neiss
    Abstract:

    Recovery after peripheral nerve injury depends not only on the amount of reinnervation, but also on its accuracy. The rat sciatic nerve was subjected to an 8 mm long gap lesion repaired either by autograft (AG, n = 6) or tubulization with impermeable Silicone Tube (SIL, n = 6) or permeable Tube of poly-L-lactide-epsilon-caprolactone (PLC, n = 8). Recordings of the compound muscle action potential (CMAP) from gastrocnemius (mGC), tibialis anterior (mTA) and plantar (mPL) muscles were performed 90 days after injury to assess the amount of muscle reinnervation. The CMAP amplitude achieved in mGC, mTA and mPL was similar in after nerve autograft (39%, 42%, 22% of control values) and PLC Tube implantation (37%, 36%, 24%) but lower with SIL Tube (29%, 30%, 14%). The nerve fascicles projecting into each of these muscles were then transected and retrograde tracers (Fluoro Gold, Fast Blue, DiI) were applied to quantify the percentage of motoneurons with single or multiple branches to different targets. The total number of labeled motoneurons for the three muscles did not differ in autografted rats (1186 +/- 56; mean +/- SEM) with respect to controls (1238 +/- 82), but was reduced with PLC Tube (802 +/- 101) and SIL Tube (935 +/- 213). The percentage of neurons with multiple projections was lower after autograft and PLC Tube (6%) than with SIL Tube (10%). Considering the higher CMAP amplitude and lower number of neurons with multiple projections, PLC nerve conduits seem superior to SIL Tubes and a suitable alternative to autografts for the repair of long gaps.

  • superior muscle reinnervation after autologous nerve graft or poly l lactide caprolactone plc Tube implantation in comparison to Silicone Tube repair
    Journal of Neuroscience Research, 2001
    Co-Authors: Antoni Valerocabre, Konstantin Tsironis, Emmanouil Skouras, Gabriele Perego, Xavier Navarro, Wolfram F Neiss
    Abstract:

    Recovery after peripheral nerve injury depends not only on the amount of reinnervation, but also on its accuracy. The rat sciatic nerve was subjected to an 8 mm long gap lesion repaired either by autograft (AG, n = 6) or tubulization with impermeable Silicone Tube (SIL, n = 6) or permeable Tube of poly-L-lactide-ϵ-caprolactone (PLC, n = 8). Recordings of the compound muscle action potential (CMAP) from gastrocnemius (mGC), tibialis anterior (mTA) and plantar (mPL) muscles were performed 90 days after injury to assess the amount of muscle reinnervation. The CMAP amplitude achieved in mGC, mTA and mPL was similar in after nerve autograft (39%, 42%, 22% of control values) and PLC Tube implantation (37%, 36%, 24%) but lower with SIL Tube (29%, 30%, 14%). The nerve fascicles projecting into each of these muscles were then transected and retrograde tracers (Fluoro Gold, Fast Blue, DiI) were applied to quantify the percentage of motoneurons with single or multiple branches to different targets. The total number of labeled motoneurons for the three muscles did not differ in autografted rats (1186 ± 56; mean ± SEM) with respect to controls (1238 ± 82), but was reduced with PLC Tube (802 ± 101) and SIL Tube (935 ± 213). The percentage of neurons with multiple projections was lower after autograft and PLC Tube (6%) than with SIL Tube (10%). Considering the higher CMAP amplitude and lower number of neurons with multiple projections, PLC nerve conduits seem superior to SIL Tubes and a suitable alternative to autografts for the repair of long gaps. J. Neurosci. Res. 63:214–223, 2001. © 2001 Wiley-Liss, Inc.

Soichiro Yamamoto - One of the best experts on this subject based on the ideXlab platform.

  • prevention and treatment of amputation neuroma by an atelocollagen Tube in rat sciatic nerves
    Journal of Biomedical Materials Research Part B, 2005
    Co-Authors: Yasuo Sakai, Koji Ryoke, Yuji Uchio, Mitsuo Ochi, Soichiro Yamamoto
    Abstract:

    To evaluate the potential of the atelocollagen Tube as a cap for amputation neuromas, the histological and histochemical characteristics of the neuroma and spinal cord were compared with those following Silicone capping. Four weeks after the transection of 18 rat sciatic nerves, the amputated neuroma was resected, and the nerve stump inserted into an atelocollagen or Silicone Tube. The histological changes in the nerve ends and c-fos expression in the dorsal horn of the fourth lumbar spinal cord were evaluated at 4 weeks postoperatively. The regenerated nerve structure in the atelocollagen or Silicone Tube was very thin. In contrast, a typical bulbous neuroma was observed in the control group (the nerve stump was left in place). The atelocollagen and Silicone Tube groups demonstrated fewer c-fos–expressed cells in the spinal cord than the controls. These results suggest that capping by an atelocollagen Tube, like that by a Silicone Tube, might successfully prevent an amputated neuroma from forming, and suppress induced pain. The atelocollagen Tube may be a promising biomaterial for the prevention or treatment of a painful amputation neuroma. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater

  • prevention and treatment of amputation neuroma by an atelocollagen Tube in rat sciatic nerves
    Journal of Biomedical Materials Research, 2005
    Co-Authors: Yasuo Sakai, Koji Ryoke, Yuji Uchio, Mitsuo Ochi, Soichiro Yamamoto
    Abstract:

    To evaluate the potential of the atelocollagen Tube as a cap for amputation neuromas, the histological and histochemical characteristics of the neuroma and spinal cord were compared with those following Silicone capping. Four weeks after the transection of 18 rat sciatic nerves, the amputated neuroma was resected, and the nerve stump inserted into an atelocollagen or Silicone Tube. The histological changes in the nerve ends and c-fos expression in the dorsal horn of the fourth lumbar spinal cord were evaluated at 4 weeks postoperatively. The regenerated nerve structure in the atelocollagen or Silicone Tube was very thin. In contrast, a typical bulbous neuroma was observed in the control group (the nerve stump was left in place). The atelocollagen and Silicone Tube groups demonstrated fewer c-fos-expressed cells in the spinal cord than the controls. These results suggest that capping by an atelocollagen Tube, like that by a Silicone Tube, might successfully prevent an amputated neuroma from forming, and suppress induced pain. The atelocollagen Tube may be a promising biomaterial for the prevention or treatment of a painful amputation neuroma.

Mitsuo Ochi - One of the best experts on this subject based on the ideXlab platform.

  • the autotomy relief effect of a Silicone Tube covering the proximal nerve stump
    Journal of Orthopaedic Research, 2006
    Co-Authors: Teruaki Okuda, Osamu Ishida, Yoshinori Fujimoto, Nobuhiro Tanaka, Atsuko Inoue, Yoshihiro Nakata, Mitsuo Ochi
    Abstract:

    A technique of covering the proximal nerve stump (PNS) has been reported as a preventative method or treatment for neuroma. However, its detailed pain relief mechanism remains unknown. We created a Silicone Tube model in which the PNS of the rat sciatic nerve was introduced into the Tube, whereas the controls had no Tube. The score of autotomy observed in the Tube group was lower than that in the control group at 3 days to 2 weeks after surgery, which suggested that the Silicone Tube had pain-like behavior inhibitory action. To elucidate the mechanism of autotomy inhibition, immunohistochemistry and Toluidine blue staining were performed in the PNS. The increase in S-100-immunoreactivity (IR) including Schwann cells was inhibited at 1 week after surgery in the Tube group, and the increase in the number of macrophages shown by ED-1-IR at 1, 2, and 4 weeks after surgery was similarly inhibited. Toluidine blue staining showed that the increase in the number of mast cells was inhibited at 1, 2, and 4 weeks after surgery and in the number of lymphocytes at 1 and 2 weeks after surgery in the Tube group. Therefore, blocking of the infiltration of inflammatory cells into the PNS by the Silicone Tube was thought to be the mechanism of autotomy inhibition. To further explore details of this mechanism, the expression of nerve growth factor (NGF), production of which is induced by inflammatory cells and of the NGF receptor TrkA was examined in the PNS and the dorsal root ganglion using immunohistochemistry and a ribonuclease protection assay. In the PNS, the increase in NGF-IR was inhibited at 1, 2, and 4 weeks after surgery in the Tube group, suggesting that this could be one of the pain-like behavior inhibitory effects.

  • prevention and treatment of amputation neuroma by an atelocollagen Tube in rat sciatic nerves
    Journal of Biomedical Materials Research Part B, 2005
    Co-Authors: Yasuo Sakai, Koji Ryoke, Yuji Uchio, Mitsuo Ochi, Soichiro Yamamoto
    Abstract:

    To evaluate the potential of the atelocollagen Tube as a cap for amputation neuromas, the histological and histochemical characteristics of the neuroma and spinal cord were compared with those following Silicone capping. Four weeks after the transection of 18 rat sciatic nerves, the amputated neuroma was resected, and the nerve stump inserted into an atelocollagen or Silicone Tube. The histological changes in the nerve ends and c-fos expression in the dorsal horn of the fourth lumbar spinal cord were evaluated at 4 weeks postoperatively. The regenerated nerve structure in the atelocollagen or Silicone Tube was very thin. In contrast, a typical bulbous neuroma was observed in the control group (the nerve stump was left in place). The atelocollagen and Silicone Tube groups demonstrated fewer c-fos–expressed cells in the spinal cord than the controls. These results suggest that capping by an atelocollagen Tube, like that by a Silicone Tube, might successfully prevent an amputated neuroma from forming, and suppress induced pain. The atelocollagen Tube may be a promising biomaterial for the prevention or treatment of a painful amputation neuroma. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater

  • prevention and treatment of amputation neuroma by an atelocollagen Tube in rat sciatic nerves
    Journal of Biomedical Materials Research, 2005
    Co-Authors: Yasuo Sakai, Koji Ryoke, Yuji Uchio, Mitsuo Ochi, Soichiro Yamamoto
    Abstract:

    To evaluate the potential of the atelocollagen Tube as a cap for amputation neuromas, the histological and histochemical characteristics of the neuroma and spinal cord were compared with those following Silicone capping. Four weeks after the transection of 18 rat sciatic nerves, the amputated neuroma was resected, and the nerve stump inserted into an atelocollagen or Silicone Tube. The histological changes in the nerve ends and c-fos expression in the dorsal horn of the fourth lumbar spinal cord were evaluated at 4 weeks postoperatively. The regenerated nerve structure in the atelocollagen or Silicone Tube was very thin. In contrast, a typical bulbous neuroma was observed in the control group (the nerve stump was left in place). The atelocollagen and Silicone Tube groups demonstrated fewer c-fos-expressed cells in the spinal cord than the controls. These results suggest that capping by an atelocollagen Tube, like that by a Silicone Tube, might successfully prevent an amputated neuroma from forming, and suppress induced pain. The atelocollagen Tube may be a promising biomaterial for the prevention or treatment of a painful amputation neuroma.

Antoni Valerocabre - One of the best experts on this subject based on the ideXlab platform.

  • superior muscle reinnervation after autologous nerve graft or poly l lactide epsilon caprolactone plc Tube implantation in comparison to Silicone Tube repair
    Journal of Neuroscience Research, 2001
    Co-Authors: Antoni Valerocabre, Konstantin Tsironis, Emmanouil Skouras, Gabriele Perego, Xavier Navarro, Wolfram F Neiss
    Abstract:

    Recovery after peripheral nerve injury depends not only on the amount of reinnervation, but also on its accuracy. The rat sciatic nerve was subjected to an 8 mm long gap lesion repaired either by autograft (AG, n = 6) or tubulization with impermeable Silicone Tube (SIL, n = 6) or permeable Tube of poly-L-lactide-epsilon-caprolactone (PLC, n = 8). Recordings of the compound muscle action potential (CMAP) from gastrocnemius (mGC), tibialis anterior (mTA) and plantar (mPL) muscles were performed 90 days after injury to assess the amount of muscle reinnervation. The CMAP amplitude achieved in mGC, mTA and mPL was similar in after nerve autograft (39%, 42%, 22% of control values) and PLC Tube implantation (37%, 36%, 24%) but lower with SIL Tube (29%, 30%, 14%). The nerve fascicles projecting into each of these muscles were then transected and retrograde tracers (Fluoro Gold, Fast Blue, DiI) were applied to quantify the percentage of motoneurons with single or multiple branches to different targets. The total number of labeled motoneurons for the three muscles did not differ in autografted rats (1186 +/- 56; mean +/- SEM) with respect to controls (1238 +/- 82), but was reduced with PLC Tube (802 +/- 101) and SIL Tube (935 +/- 213). The percentage of neurons with multiple projections was lower after autograft and PLC Tube (6%) than with SIL Tube (10%). Considering the higher CMAP amplitude and lower number of neurons with multiple projections, PLC nerve conduits seem superior to SIL Tubes and a suitable alternative to autografts for the repair of long gaps.

  • superior muscle reinnervation after autologous nerve graft or poly l lactide caprolactone plc Tube implantation in comparison to Silicone Tube repair
    Journal of Neuroscience Research, 2001
    Co-Authors: Antoni Valerocabre, Konstantin Tsironis, Emmanouil Skouras, Gabriele Perego, Xavier Navarro, Wolfram F Neiss
    Abstract:

    Recovery after peripheral nerve injury depends not only on the amount of reinnervation, but also on its accuracy. The rat sciatic nerve was subjected to an 8 mm long gap lesion repaired either by autograft (AG, n = 6) or tubulization with impermeable Silicone Tube (SIL, n = 6) or permeable Tube of poly-L-lactide-ϵ-caprolactone (PLC, n = 8). Recordings of the compound muscle action potential (CMAP) from gastrocnemius (mGC), tibialis anterior (mTA) and plantar (mPL) muscles were performed 90 days after injury to assess the amount of muscle reinnervation. The CMAP amplitude achieved in mGC, mTA and mPL was similar in after nerve autograft (39%, 42%, 22% of control values) and PLC Tube implantation (37%, 36%, 24%) but lower with SIL Tube (29%, 30%, 14%). The nerve fascicles projecting into each of these muscles were then transected and retrograde tracers (Fluoro Gold, Fast Blue, DiI) were applied to quantify the percentage of motoneurons with single or multiple branches to different targets. The total number of labeled motoneurons for the three muscles did not differ in autografted rats (1186 ± 56; mean ± SEM) with respect to controls (1238 ± 82), but was reduced with PLC Tube (802 ± 101) and SIL Tube (935 ± 213). The percentage of neurons with multiple projections was lower after autograft and PLC Tube (6%) than with SIL Tube (10%). Considering the higher CMAP amplitude and lower number of neurons with multiple projections, PLC nerve conduits seem superior to SIL Tubes and a suitable alternative to autografts for the repair of long gaps. J. Neurosci. Res. 63:214–223, 2001. © 2001 Wiley-Liss, Inc.

Yasuo Sakai - One of the best experts on this subject based on the ideXlab platform.

  • prevention and treatment of amputation neuroma by an atelocollagen Tube in rat sciatic nerves
    Journal of Biomedical Materials Research Part B, 2005
    Co-Authors: Yasuo Sakai, Koji Ryoke, Yuji Uchio, Mitsuo Ochi, Soichiro Yamamoto
    Abstract:

    To evaluate the potential of the atelocollagen Tube as a cap for amputation neuromas, the histological and histochemical characteristics of the neuroma and spinal cord were compared with those following Silicone capping. Four weeks after the transection of 18 rat sciatic nerves, the amputated neuroma was resected, and the nerve stump inserted into an atelocollagen or Silicone Tube. The histological changes in the nerve ends and c-fos expression in the dorsal horn of the fourth lumbar spinal cord were evaluated at 4 weeks postoperatively. The regenerated nerve structure in the atelocollagen or Silicone Tube was very thin. In contrast, a typical bulbous neuroma was observed in the control group (the nerve stump was left in place). The atelocollagen and Silicone Tube groups demonstrated fewer c-fos–expressed cells in the spinal cord than the controls. These results suggest that capping by an atelocollagen Tube, like that by a Silicone Tube, might successfully prevent an amputated neuroma from forming, and suppress induced pain. The atelocollagen Tube may be a promising biomaterial for the prevention or treatment of a painful amputation neuroma. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater

  • prevention and treatment of amputation neuroma by an atelocollagen Tube in rat sciatic nerves
    Journal of Biomedical Materials Research, 2005
    Co-Authors: Yasuo Sakai, Koji Ryoke, Yuji Uchio, Mitsuo Ochi, Soichiro Yamamoto
    Abstract:

    To evaluate the potential of the atelocollagen Tube as a cap for amputation neuromas, the histological and histochemical characteristics of the neuroma and spinal cord were compared with those following Silicone capping. Four weeks after the transection of 18 rat sciatic nerves, the amputated neuroma was resected, and the nerve stump inserted into an atelocollagen or Silicone Tube. The histological changes in the nerve ends and c-fos expression in the dorsal horn of the fourth lumbar spinal cord were evaluated at 4 weeks postoperatively. The regenerated nerve structure in the atelocollagen or Silicone Tube was very thin. In contrast, a typical bulbous neuroma was observed in the control group (the nerve stump was left in place). The atelocollagen and Silicone Tube groups demonstrated fewer c-fos-expressed cells in the spinal cord than the controls. These results suggest that capping by an atelocollagen Tube, like that by a Silicone Tube, might successfully prevent an amputated neuroma from forming, and suppress induced pain. The atelocollagen Tube may be a promising biomaterial for the prevention or treatment of a painful amputation neuroma.