The Experts below are selected from a list of 288 Experts worldwide ranked by ideXlab platform

Carol L. Wells - One of the best experts on this subject based on the ideXlab platform.

  • gentamicin promotes staphylococcus aureus biofilms on Silk Suture
    Journal of Surgical Research, 2011
    Co-Authors: Donavon J. Hess, Michelle J Henrystanley, Carol L. Wells
    Abstract:

    Background Communities of bacteria, termed biofilms, develop on biotic and abiotic surfaces, including medical devices and surgical Suture. Biofilm-associated bacteria are typically recalcitrant to antibiotic therapy, and the effects of antibiotics on microbial biofilms are not clearly understood. There is emerging evidence that under specific conditions, aminoglycosides may actually promote biofilm development. Experiments were designed to study the effects of gentamicin on Suture-associated Staphylococcus aureus biofilms. Materials and Methods S. aureus biofilms were formed after 24 h incubation of bacteria with Silk Suture. Susceptibility of planktonic S. aureus (from broth culture) to gentamicin was compared with the susceptibility of cells from mechanically dispersed S. aureus biofilms. Subinhibitory and inhibitory concentrations of gentamicin were subsequently incubated with intact Suture-associated biofilms. S. aureus viability and metabolic capacity were assessed, and biofilm biomass was quantified with crystal violet (binds negatively charged surface molecules) and with the nucleic acid stain Syto 9. Scanning electron microscopy was used to assess the effect of gentamicin on the ultrastructure of Suture-associated S. aureus biofilms. Results Planktonic cells and S. aureus cells from mechanically dispersed biofilms had similar susceptibility to gentamicin. However, after incubation of high concentrations of gentamicin with intact biofilms, high numbers of S. aureus remained both viable and metabolically active; biofilm biomass was increased and biofilm ultrastructure showed staphylococcal cells within copious amounts of extracellular material. Conclusion Gentamicin does not effectively kill S. aureus within intact Suture-associated biofilms, and gentamicin also promotes the biomass of S. aureus biofilms.

  • Gentamicin promotes Staphylococcus aureus biofilms on Silk Suture
    The Journal of surgical research, 2011
    Co-Authors: Donavon J. Hess, Michelle J. Henry-stanley, Carol L. Wells
    Abstract:

    Communities of bacteria, termed biofilms, develop on biotic and abiotic surfaces, including medical devices and surgical Suture. Biofilm-associated bacteria are typically recalcitrant to antibiotic therapy, and the effects of antibiotics on microbial biofilms are not clearly understood. There is emerging evidence that under specific conditions, aminoglycosides may actually promote biofilm development. Experiments were designed to study the effects of gentamicin on Suture-associated Staphylococcus aureus biofilms. S. aureus biofilms were formed after 24 h incubation of bacteria with Silk Suture. Susceptibility of planktonic S. aureus (from broth culture) to gentamicin was compared with the susceptibility of cells from mechanically dispersed S. aureus biofilms. Subinhibitory and inhibitory concentrations of gentamicin were subsequently incubated with intact Suture-associated biofilms. S. aureus viability and metabolic capacity were assessed, and biofilm biomass was quantified with crystal violet (binds negatively charged surface molecules) and with the nucleic acid stain Syto 9. Scanning electron microscopy was used to assess the effect of gentamicin on the ultrastructure of Suture-associated S. aureus biofilms. Planktonic cells and S. aureus cells from mechanically dispersed biofilms had similar susceptibility to gentamicin. However, after incubation of high concentrations of gentamicin with intact biofilms, high numbers of S. aureus remained both viable and metabolically active; biofilm biomass was increased and biofilm ultrastructure showed staphylococcal cells within copious amounts of extracellular material. Gentamicin does not effectively kill S. aureus within intact Suture-associated biofilms, and gentamicin also promotes the biomass of S. aureus biofilms. Copyright © 2011 Elsevier Inc. All rights reserved.

Donavon J. Hess - One of the best experts on this subject based on the ideXlab platform.

  • gentamicin promotes staphylococcus aureus biofilms on Silk Suture
    Journal of Surgical Research, 2011
    Co-Authors: Donavon J. Hess, Michelle J Henrystanley, Carol L. Wells
    Abstract:

    Background Communities of bacteria, termed biofilms, develop on biotic and abiotic surfaces, including medical devices and surgical Suture. Biofilm-associated bacteria are typically recalcitrant to antibiotic therapy, and the effects of antibiotics on microbial biofilms are not clearly understood. There is emerging evidence that under specific conditions, aminoglycosides may actually promote biofilm development. Experiments were designed to study the effects of gentamicin on Suture-associated Staphylococcus aureus biofilms. Materials and Methods S. aureus biofilms were formed after 24 h incubation of bacteria with Silk Suture. Susceptibility of planktonic S. aureus (from broth culture) to gentamicin was compared with the susceptibility of cells from mechanically dispersed S. aureus biofilms. Subinhibitory and inhibitory concentrations of gentamicin were subsequently incubated with intact Suture-associated biofilms. S. aureus viability and metabolic capacity were assessed, and biofilm biomass was quantified with crystal violet (binds negatively charged surface molecules) and with the nucleic acid stain Syto 9. Scanning electron microscopy was used to assess the effect of gentamicin on the ultrastructure of Suture-associated S. aureus biofilms. Results Planktonic cells and S. aureus cells from mechanically dispersed biofilms had similar susceptibility to gentamicin. However, after incubation of high concentrations of gentamicin with intact biofilms, high numbers of S. aureus remained both viable and metabolically active; biofilm biomass was increased and biofilm ultrastructure showed staphylococcal cells within copious amounts of extracellular material. Conclusion Gentamicin does not effectively kill S. aureus within intact Suture-associated biofilms, and gentamicin also promotes the biomass of S. aureus biofilms.

  • Gentamicin promotes Staphylococcus aureus biofilms on Silk Suture
    The Journal of surgical research, 2011
    Co-Authors: Donavon J. Hess, Michelle J. Henry-stanley, Carol L. Wells
    Abstract:

    Communities of bacteria, termed biofilms, develop on biotic and abiotic surfaces, including medical devices and surgical Suture. Biofilm-associated bacteria are typically recalcitrant to antibiotic therapy, and the effects of antibiotics on microbial biofilms are not clearly understood. There is emerging evidence that under specific conditions, aminoglycosides may actually promote biofilm development. Experiments were designed to study the effects of gentamicin on Suture-associated Staphylococcus aureus biofilms. S. aureus biofilms were formed after 24 h incubation of bacteria with Silk Suture. Susceptibility of planktonic S. aureus (from broth culture) to gentamicin was compared with the susceptibility of cells from mechanically dispersed S. aureus biofilms. Subinhibitory and inhibitory concentrations of gentamicin were subsequently incubated with intact Suture-associated biofilms. S. aureus viability and metabolic capacity were assessed, and biofilm biomass was quantified with crystal violet (binds negatively charged surface molecules) and with the nucleic acid stain Syto 9. Scanning electron microscopy was used to assess the effect of gentamicin on the ultrastructure of Suture-associated S. aureus biofilms. Planktonic cells and S. aureus cells from mechanically dispersed biofilms had similar susceptibility to gentamicin. However, after incubation of high concentrations of gentamicin with intact biofilms, high numbers of S. aureus remained both viable and metabolically active; biofilm biomass was increased and biofilm ultrastructure showed staphylococcal cells within copious amounts of extracellular material. Gentamicin does not effectively kill S. aureus within intact Suture-associated biofilms, and gentamicin also promotes the biomass of S. aureus biofilms. Copyright © 2011 Elsevier Inc. All rights reserved.

W L Adeyemo - One of the best experts on this subject based on the ideXlab platform.

  • cyanoacrylate tissue adhesive or Silk Suture for closure of surgical wound following removal of an impacted mandibular third molar a randomized controlled study
    Journal of Cranio-maxillofacial Surgery, 2019
    Co-Authors: Afisu A Oladega, Olutayo James, W L Adeyemo
    Abstract:

    Abstract Aim The aim of the study was to compare postoperative sequelae and wound healing outcome following closure of surgical wound with either cyanoacrylate tissue adhesive or Silk Suture. Methods Subjects with mesio-angularly impacted mandibular third molar were allocated randomly into 2 equal groups. The control group had wound closure with Silk Suture and study group with cyanoacrylate tissue adhesive. Subjects were followed up for 7 postoperative days. Postoperative pain, swelling, trismus, bleeding, wound dehiscence and wound infection were evaluated. Results Sixty subjects in each group completed the study. No significant difference was observed in the mean postoperative pain, swelling, trismus, wound dehiscence and infection between the 2 groups. There was a statistically significant difference in postoperative bleeding between the 2 groups on postoperative day 1, with more bleeding in the control group. Conclusions This study shows that cyanoacrylate tissue adhesive compares favourably with Silk Suture as a wound closure material. In addition, cyanoacrylate tissue adhesive seems to have beneficial haemostatic effect on postoperative bleeding.

  • Cyanoacrylate tissue adhesive or Silk Suture for closure of surgical wound following removal of an impacted mandibular third molar: A randomized controlled study
    Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery, 2018
    Co-Authors: Afisu A Oladega, Olutayo James, W L Adeyemo
    Abstract:

    The aim of the study was to compare postoperative sequelae and wound healing outcome following closure of surgical wound with either cyanoacrylate tissue adhesive or Silk Suture. Subjects with mesio-angularly impacted mandibular third molar were allocated randomly into 2 equal groups. The control group had wound closure with Silk Suture and study group with cyanoacrylate tissue adhesive. Subjects were followed up for 7 postoperative days. Postoperative pain, swelling, trismus, bleeding, wound dehiscence and wound infection were evaluated. Sixty subjects in each group completed the study. No significant difference was observed in the mean postoperative pain, swelling, trismus, wound dehiscence and infection between the 2 groups. There was a statistically significant difference in postoperative bleeding between the 2 groups on postoperative day 1, with more bleeding in the control group. This study shows that cyanoacrylate tissue adhesive compares favourably with Silk Suture as a wound closure material. In addition, cyanoacrylate tissue adhesive seems to have beneficial haemostatic effect on postoperative bleeding. Copyright © 2018 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.

Afisu A Oladega - One of the best experts on this subject based on the ideXlab platform.

  • cyanoacrylate tissue adhesive or Silk Suture for closure of surgical wound following removal of an impacted mandibular third molar a randomized controlled study
    Journal of Cranio-maxillofacial Surgery, 2019
    Co-Authors: Afisu A Oladega, Olutayo James, W L Adeyemo
    Abstract:

    Abstract Aim The aim of the study was to compare postoperative sequelae and wound healing outcome following closure of surgical wound with either cyanoacrylate tissue adhesive or Silk Suture. Methods Subjects with mesio-angularly impacted mandibular third molar were allocated randomly into 2 equal groups. The control group had wound closure with Silk Suture and study group with cyanoacrylate tissue adhesive. Subjects were followed up for 7 postoperative days. Postoperative pain, swelling, trismus, bleeding, wound dehiscence and wound infection were evaluated. Results Sixty subjects in each group completed the study. No significant difference was observed in the mean postoperative pain, swelling, trismus, wound dehiscence and infection between the 2 groups. There was a statistically significant difference in postoperative bleeding between the 2 groups on postoperative day 1, with more bleeding in the control group. Conclusions This study shows that cyanoacrylate tissue adhesive compares favourably with Silk Suture as a wound closure material. In addition, cyanoacrylate tissue adhesive seems to have beneficial haemostatic effect on postoperative bleeding.

  • Cyanoacrylate tissue adhesive or Silk Suture for closure of surgical wound following removal of an impacted mandibular third molar: A randomized controlled study
    Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery, 2018
    Co-Authors: Afisu A Oladega, Olutayo James, W L Adeyemo
    Abstract:

    The aim of the study was to compare postoperative sequelae and wound healing outcome following closure of surgical wound with either cyanoacrylate tissue adhesive or Silk Suture. Subjects with mesio-angularly impacted mandibular third molar were allocated randomly into 2 equal groups. The control group had wound closure with Silk Suture and study group with cyanoacrylate tissue adhesive. Subjects were followed up for 7 postoperative days. Postoperative pain, swelling, trismus, bleeding, wound dehiscence and wound infection were evaluated. Sixty subjects in each group completed the study. No significant difference was observed in the mean postoperative pain, swelling, trismus, wound dehiscence and infection between the 2 groups. There was a statistically significant difference in postoperative bleeding between the 2 groups on postoperative day 1, with more bleeding in the control group. This study shows that cyanoacrylate tissue adhesive compares favourably with Silk Suture as a wound closure material. In addition, cyanoacrylate tissue adhesive seems to have beneficial haemostatic effect on postoperative bleeding. Copyright © 2018 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.

Daniel Ruefenacht - One of the best experts on this subject based on the ideXlab platform.

  • Preoperative Silk Suture embolization of cerebral and dural arteriovenous malformations
    Neurosurgical focus, 2001
    Co-Authors: Amir R. Dehdashti, M. Muster, Alain Reverdin, Nicolas De Tribolet, Daniel Ruefenacht
    Abstract:

    The aim of this study was to evaluate the use of Silk Sutures as a medical implant when applied for the embolization of cerebral and dural arteriovenous malformations (AVMs). The facility of surgery and the clinical significance of complications related to preoperative Silk Suture embolization were evaluated immediately after surgery and at long-term follow up. Thirty-four patients harboring 29 cerebral and five dural AVMs underwent embolization in which Silk alone or in association with other agents was used. Medical and radiological records obtained in these 34 patients were reviewed retrospectively. The cerebral AVMs were classified according to the Spetzler-Martin grading system and the dural AVMs to the Djindjian grading system. The facility of the resection and the adverse outcomes, including new neurological deficits, hemorrhage, and fever, as well as histopathological evidence of vessel inflammatory changes, were determined in each case. In all 23 surgical cases, the AVM could be easily manipulated and excised. New temporary neurological deficits occurred in three patients. A high Spetzler-Martin grade was not associated with a higher incidence of new neurological deficits. One delayed-onset hemorrhage was detected after embolization. Fever was present in 24% of the patients. No sign of significant vasculitis or perivascular inflammation was found on radiological or histopathological examination. Silk Sutures are safe embolic agents especially for proximal occlusion of AVM feeding vessels. New permanent neurological deficits were not encountered in this series. Fever was considered to be a minor, temporary side effect of Silk Suture embolization.

  • preoperative Silk Suture embolization of cerebral and dural arteriovenous malformations
    Neurosurgical Focus, 2001
    Co-Authors: Amir R. Dehdashti, M. Muster, Alain Reverdin, Nicolas De Tribolet, Daniel Ruefenacht
    Abstract:

    Object. The aim of this study was to evaluate the use of Silk Sutures as a medical implant when applied for the embolization of cerebral and dural arteriovenous malformations (AVMs). The facility of surgery and the clinical significance of complications related to preoperative Silk Suture embolization were evaluated immediately after surgery and at long-term follow up. Methods. Thirty-four patients harboring 29 cerebral and five dural AVMs underwent embolization in which Silk alone or in association with other agents was used. Medical and radiological records obtained in these 34 patients were reviewed retrospectively. The cerebral AVMs were classified according to the Spetzler–Martin grading system and the dural AVMs to the Djindjian grading system. The facility of the resection and the adverse outcomes, including new neurological deficits, hemorrhage, and fever, as well as histopathological evidence of vessel inflammatory changes, were determined in each case. In all 23 surgical cases, the AVM could be easily manipulated and excised. New temporary neurological deficits occurred in three patients. A high Spetzler–Martin grade was not associated with a higher incidence of new neurological deficits. One delayed-onset hemorrhage was detected after embolization. Fever was present in 24% of the patients. No sign of significant vasculitis or perivascular inflammation was found on radiological or histopathological examination. Conclusions. Silk Sutures are safe embolic agents especially for proximal occlusion of AVM feeding vessels. New permanent neurological deficits were not encountered in this series. Fever was considered to be a minor, temporary side effect of Silk Suture embolization.