The Experts below are selected from a list of 189 Experts worldwide ranked by ideXlab platform

Litai Jin - One of the best experts on this subject based on the ideXlab platform.

  • negative Staining of lipopolysaccharides on polyacrylamide gels by using eosin b
    Analytical Biochemistry, 2012
    Co-Authors: Zhongxin Zhu, Weitao Cong, Jungkap Choi, Litai Jin, Xuan Zhou, Mao Chen
    Abstract:

    A sensitive and simple technique for the negative detection of lipopolysaccharides (LPSs) following polyacrylamide gel electrophoresis (PAGE) using eosin B (EB) was developed. After electrophoresis, gels were fixed, Stained, and developed within 30 min to achieve transparent and colorless LPS bands under opaque gel matrix background. As low as 20 to 40 ng of total LPSs could be detected, which is 4-fold more sensitive than those of the widely used Silver Stain developed by Fomsgaard and coworkers and imidazole-zinc (IZ) negative Stain. For its sensitivity and brevity, this Stain may be a practical method for LPS determination in the routine laboratory.

  • An improved Silver Stain for the visualization of lipopolysaccharides on polyacrylamide gels.
    Electrophoresis, 2012
    Co-Authors: Zhongxin Zhu, Weitao Cong, Xi Wang, Litai Jin
    Abstract:

    A sensitive, brief, and user-friendly Silver Stain to meet the needs in high-efficiency detection of lipopolysaccharides (LPS) on polyacrylamide gels is described. In this study, the most commonly used formaldehyde-based LPS Silver Stain, which is potentially hazardous to the operator, is replaced by ascorbic acid (Vc) in alkaline sodium thiosulfate solution. It takes only about 35 min to complete all the protocol, with a detection limit of 4 ng of total LPS. The results indicate that this user-friendly method could be a good choice for LPS visualization on polyacrylamide gels.

Weitao Cong - One of the best experts on this subject based on the ideXlab platform.

  • advanced negative detection method comparable to Silver Stain for sds page separated proteins detection
    Analytical Biochemistry, 2016
    Co-Authors: Xu Wang, Weitao Cong, Sunyoung Hwang, Jungkap Choi
    Abstract:

    Abstract In order to achieve an easy, rapid and sensitive protocol to detect proteins in polyacrylamide gel, an advanced negative detection method comparable to Silver Stain is described. When a gel was incubated with Phloxine B and followed by the development in acidic solution, the zones where forming protein-dye complex were selectively transparent, unlike opaque gel background. Within 50 min after electrophoresis, down to 0.1–0.4 ng of gel-separated proteins (similar with Silver Stain) could be observed, without labor-intensive and time-consuming procedure. Comparing with the most common negative Stain method, Imidazole-zinc Stain, Phloxine B Stain has been shown higher sensitivity and distinct contrast between the transparent protein bands/spots and opaque background than those; furthermore, it is no longer necessary to concern about retention time of observation. This technique may provide a sensitive and practical choice for proteomics researches.

  • negative Staining of lipopolysaccharides on polyacrylamide gels by using eosin b
    Analytical Biochemistry, 2012
    Co-Authors: Zhongxin Zhu, Weitao Cong, Jungkap Choi, Litai Jin, Xuan Zhou, Mao Chen
    Abstract:

    A sensitive and simple technique for the negative detection of lipopolysaccharides (LPSs) following polyacrylamide gel electrophoresis (PAGE) using eosin B (EB) was developed. After electrophoresis, gels were fixed, Stained, and developed within 30 min to achieve transparent and colorless LPS bands under opaque gel matrix background. As low as 20 to 40 ng of total LPSs could be detected, which is 4-fold more sensitive than those of the widely used Silver Stain developed by Fomsgaard and coworkers and imidazole-zinc (IZ) negative Stain. For its sensitivity and brevity, this Stain may be a practical method for LPS determination in the routine laboratory.

  • An improved Silver Stain for the visualization of lipopolysaccharides on polyacrylamide gels.
    Electrophoresis, 2012
    Co-Authors: Zhongxin Zhu, Weitao Cong, Xi Wang, Litai Jin
    Abstract:

    A sensitive, brief, and user-friendly Silver Stain to meet the needs in high-efficiency detection of lipopolysaccharides (LPS) on polyacrylamide gels is described. In this study, the most commonly used formaldehyde-based LPS Silver Stain, which is potentially hazardous to the operator, is replaced by ascorbic acid (Vc) in alkaline sodium thiosulfate solution. It takes only about 35 min to complete all the protocol, with a detection limit of 4 ng of total LPS. The results indicate that this user-friendly method could be a good choice for LPS visualization on polyacrylamide gels.

Jungkap Choi - One of the best experts on this subject based on the ideXlab platform.

  • advanced negative detection method comparable to Silver Stain for sds page separated proteins detection
    Analytical Biochemistry, 2016
    Co-Authors: Xu Wang, Weitao Cong, Sunyoung Hwang, Jungkap Choi
    Abstract:

    Abstract In order to achieve an easy, rapid and sensitive protocol to detect proteins in polyacrylamide gel, an advanced negative detection method comparable to Silver Stain is described. When a gel was incubated with Phloxine B and followed by the development in acidic solution, the zones where forming protein-dye complex were selectively transparent, unlike opaque gel background. Within 50 min after electrophoresis, down to 0.1–0.4 ng of gel-separated proteins (similar with Silver Stain) could be observed, without labor-intensive and time-consuming procedure. Comparing with the most common negative Stain method, Imidazole-zinc Stain, Phloxine B Stain has been shown higher sensitivity and distinct contrast between the transparent protein bands/spots and opaque background than those; furthermore, it is no longer necessary to concern about retention time of observation. This technique may provide a sensitive and practical choice for proteomics researches.

  • negative Staining of lipopolysaccharides on polyacrylamide gels by using eosin b
    Analytical Biochemistry, 2012
    Co-Authors: Zhongxin Zhu, Weitao Cong, Jungkap Choi, Litai Jin, Xuan Zhou, Mao Chen
    Abstract:

    A sensitive and simple technique for the negative detection of lipopolysaccharides (LPSs) following polyacrylamide gel electrophoresis (PAGE) using eosin B (EB) was developed. After electrophoresis, gels were fixed, Stained, and developed within 30 min to achieve transparent and colorless LPS bands under opaque gel matrix background. As low as 20 to 40 ng of total LPSs could be detected, which is 4-fold more sensitive than those of the widely used Silver Stain developed by Fomsgaard and coworkers and imidazole-zinc (IZ) negative Stain. For its sensitivity and brevity, this Stain may be a practical method for LPS determination in the routine laboratory.

Zhongxin Zhu - One of the best experts on this subject based on the ideXlab platform.

  • negative Staining of lipopolysaccharides on polyacrylamide gels by using eosin b
    Analytical Biochemistry, 2012
    Co-Authors: Zhongxin Zhu, Weitao Cong, Jungkap Choi, Litai Jin, Xuan Zhou, Mao Chen
    Abstract:

    A sensitive and simple technique for the negative detection of lipopolysaccharides (LPSs) following polyacrylamide gel electrophoresis (PAGE) using eosin B (EB) was developed. After electrophoresis, gels were fixed, Stained, and developed within 30 min to achieve transparent and colorless LPS bands under opaque gel matrix background. As low as 20 to 40 ng of total LPSs could be detected, which is 4-fold more sensitive than those of the widely used Silver Stain developed by Fomsgaard and coworkers and imidazole-zinc (IZ) negative Stain. For its sensitivity and brevity, this Stain may be a practical method for LPS determination in the routine laboratory.

  • An improved Silver Stain for the visualization of lipopolysaccharides on polyacrylamide gels.
    Electrophoresis, 2012
    Co-Authors: Zhongxin Zhu, Weitao Cong, Xi Wang, Litai Jin
    Abstract:

    A sensitive, brief, and user-friendly Silver Stain to meet the needs in high-efficiency detection of lipopolysaccharides (LPS) on polyacrylamide gels is described. In this study, the most commonly used formaldehyde-based LPS Silver Stain, which is potentially hazardous to the operator, is replaced by ascorbic acid (Vc) in alkaline sodium thiosulfate solution. It takes only about 35 min to complete all the protocol, with a detection limit of 4 ng of total LPS. The results indicate that this user-friendly method could be a good choice for LPS visualization on polyacrylamide gels.

James W Schumm - One of the best experts on this subject based on the ideXlab platform.

  • multiplex sets for the amplification of polymorphic short tandem repeat loci Silver Stain and fluorescence detection
    BioTechniques, 1996
    Co-Authors: Ann M Lins, Cynthia J Sprecher, Christoph Puers, James W Schumm
    Abstract:

    Multiplex PCR amplification systems were developed using well-characterized, polymorphic short tandem repeat (STR) loci. Eight loci utilized in the multiplex amplifications included HUMCSF1PO, HUMTPOX, HUMTH01, HUMVWFA31, HUMF13A01, HUMFESFPS, HUMBFXIII and HUMLIPOL. From this list, three or four non-overlapping loci were simultaneously amplified, separated by denaturing polyacrylamide gel electrophoresis and visualized using Silver Stain or fluorescence detection. The multiplex PCR amplification systems offer a non-isotopic method for rapid, simple and accurate analysis of STR loci. This high-throughput method for DNA identification has immediate and valuable application in forensic analysis, paternity determination, tissue culture strain identification and bone marrow transplantation studies.