The Experts below are selected from a list of 81 Experts worldwide ranked by ideXlab platform
J Wen - One of the best experts on this subject based on the ideXlab platform.
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Size-exclusion chromatography with on-line light scattering.
Current protocols in protein science, 2001Co-Authors: T Arakawa, J WenAbstract:This unit describes the use of size-exclusion chromatography with on-line light scattering, UV absorbance, and refractive index detectors (SEC-LS/UV/RI) to determine: (a) the molecular weight of Simple Proteins containing no carbohydrates, (b) the molecular weight of glycoProteins, and (c), most importantly, the molecular weight and stoichiometry of protein-protein complexes or protein-carbohydrate complexes. Multiangle light scattering is also discussed.
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Current Protocols in Protein Science - Size-exclusion chromatography with on-line light scattering.
Current Protocols in Protein Science, 2001Co-Authors: T Arakawa, J WenAbstract:This unit describes the use of size-exclusion chromatography with on-line light scattering, UV absorbance, and refractive index detectors (SEC-LS/UV/RI) to determine: (a) the molecular weight of Simple Proteins containing no carbohydrates, (b) the molecular weight of glycoProteins, and (c), most importantly, the molecular weight and stoichiometry of protein-protein complexes or protein-carbohydrate complexes. Multiangle light scattering is also discussed.
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size exclusion chromatography with on line light scattering absorbance and refractive index detectors for studying Proteins and their interactions
Analytical Biochemistry, 1996Co-Authors: J Wen, T Arakawa, John S PhiloAbstract:Techniques of using size-exclusion chromatography (SEC) with on-line light-scattering, uv absorbance, and refractive index detectors to characterize the polypeptide molecular weights of Simple Proteins or glycoProteins or to determine the stoichiometry of protein complexes are described. Two unique advantages of this approach over conventional SEC are that the molecular weight measurement is independent of elution position and can exclude the contributions from carbohydrates. When a protein or complex contains no carbohydrates, a two-detector method, i.e., light scattering combined with refractive index, can be used to calculate the molecular weight. When a protein contains carbohydrates, a three-detector method is used to calculate the molecular weight of polypeptide alone. Finally, a self-consistent three-detector method is used to determine the stoichiometry of a protein complex containing carbohydrates. Example applications for all these methodologies are described.
T Arakawa - One of the best experts on this subject based on the ideXlab platform.
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Size-exclusion chromatography with on-line light scattering.
Current protocols in protein science, 2001Co-Authors: T Arakawa, J WenAbstract:This unit describes the use of size-exclusion chromatography with on-line light scattering, UV absorbance, and refractive index detectors (SEC-LS/UV/RI) to determine: (a) the molecular weight of Simple Proteins containing no carbohydrates, (b) the molecular weight of glycoProteins, and (c), most importantly, the molecular weight and stoichiometry of protein-protein complexes or protein-carbohydrate complexes. Multiangle light scattering is also discussed.
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Current Protocols in Protein Science - Size-exclusion chromatography with on-line light scattering.
Current Protocols in Protein Science, 2001Co-Authors: T Arakawa, J WenAbstract:This unit describes the use of size-exclusion chromatography with on-line light scattering, UV absorbance, and refractive index detectors (SEC-LS/UV/RI) to determine: (a) the molecular weight of Simple Proteins containing no carbohydrates, (b) the molecular weight of glycoProteins, and (c), most importantly, the molecular weight and stoichiometry of protein-protein complexes or protein-carbohydrate complexes. Multiangle light scattering is also discussed.
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size exclusion chromatography with on line light scattering absorbance and refractive index detectors for studying Proteins and their interactions
Analytical Biochemistry, 1996Co-Authors: J Wen, T Arakawa, John S PhiloAbstract:Techniques of using size-exclusion chromatography (SEC) with on-line light-scattering, uv absorbance, and refractive index detectors to characterize the polypeptide molecular weights of Simple Proteins or glycoProteins or to determine the stoichiometry of protein complexes are described. Two unique advantages of this approach over conventional SEC are that the molecular weight measurement is independent of elution position and can exclude the contributions from carbohydrates. When a protein or complex contains no carbohydrates, a two-detector method, i.e., light scattering combined with refractive index, can be used to calculate the molecular weight. When a protein contains carbohydrates, a three-detector method is used to calculate the molecular weight of polypeptide alone. Finally, a self-consistent three-detector method is used to determine the stoichiometry of a protein complex containing carbohydrates. Example applications for all these methodologies are described.
John S Philo - One of the best experts on this subject based on the ideXlab platform.
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size exclusion chromatography with on line light scattering absorbance and refractive index detectors for studying Proteins and their interactions
Analytical Biochemistry, 1996Co-Authors: J Wen, T Arakawa, John S PhiloAbstract:Techniques of using size-exclusion chromatography (SEC) with on-line light-scattering, uv absorbance, and refractive index detectors to characterize the polypeptide molecular weights of Simple Proteins or glycoProteins or to determine the stoichiometry of protein complexes are described. Two unique advantages of this approach over conventional SEC are that the molecular weight measurement is independent of elution position and can exclude the contributions from carbohydrates. When a protein or complex contains no carbohydrates, a two-detector method, i.e., light scattering combined with refractive index, can be used to calculate the molecular weight. When a protein contains carbohydrates, a three-detector method is used to calculate the molecular weight of polypeptide alone. Finally, a self-consistent three-detector method is used to determine the stoichiometry of a protein complex containing carbohydrates. Example applications for all these methodologies are described.
A. M. Diwan - One of the best experts on this subject based on the ideXlab platform.
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A protocol for protein estimation that gives a nearly constant color yield with Simple Proteins and nullifies the effects of four known interfering agents: microestimation of peptide groups.
Analytical biochemistry, 1994Co-Authors: Ravi Narayan Raghupathi, A. M. DiwanAbstract:Abstract A modified version of the Lowry protocol for protein measurement is being introduced in which the increment in color due to copper-protein interactions can be directly equated to the concentration of peptide groups (the CO-NH moieties) present in the sample. The method provides an accurate estimation of protein concentrations in the range of 5-40 μg, with a sensitivity similar to that of the original assay procedure. It yields hardly any protein-to-protein variation with Simple Proteins and eliminates the interference by four compounds that commonly affect the Lowry assay. A numerical factor has been calculated that allows conversion of micromoles of peptide groups into actual micrograms of protein present in the sample.
H Chen - One of the best experts on this subject based on the ideXlab platform.
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Functional Properties of Edible Films Using Whey Protein Concentrate
Journal of Dairy Science, 1995Co-Authors: R. Banerjee, H ChenAbstract:Abstract Methodologies were developed to form edible films of Simple Proteins or protein-lipid composited using whey protein concentrate. The functional properties of whey protein concentrate films were compared with those of the films derived from sodium caseinate, potassium caseinate, calcium caseinate, and whey protein isolate. Water vapor permeability of Simple whey protein concentrate film was lower than that for films of sodium caseinate, potassium caseinate, and whey protein isolate. Composite whey protein concentrate film had the lowest water vapor permeability of all the milk protein films. The ultimate tensile strengths of Simple whey protein concentrate films were similar to those of caseinate films. Whey protein concentrate films exhibited lower puncture strengths than did films from other milk Proteins except Simple film from sodium caseinate. Whey protein concentrate and isolate films had higher elongation values than did Simple calcium caseinate films. Transmission electron microscopy revealed the presence of residual milk fat embedded in the protein matrix in whey protein concentrate films. Whey protein concentrate films had good water vapor barrier and mechanical properties that were comparable with those of films from other commercial milk Proteins.