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Sohan S Chitlange - One of the best experts on this subject based on the ideXlab platform.
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development and validation of uv spectrophotometric methods for Simultaneous Estimation of cetirizine hydrochloride and phenylephrine hydrochloride in tablets
International Journal of Pharmaceutical Sciences and Drug Research, 2012Co-Authors: Sagar B Wankhede, K A Lad, Sohan S ChitlangeAbstract:Without resolving mixtures of Cetirizine hydrochloride and Phenylephrine hydrochloride, Simultaneous Estimation has been successfully achieved by spectrophotometry. First method, Simultaneous equation method employs formation and solving of mathematical Simultaneous equation using 237.5 nm and 232.0 nm as the λmax of Phenylephrine hydrochloride and Cetirizine hydrochloride respectively in distilled water. Second method is first order derivative spectroscopy, wavelengths selected for quantitation were 232.0 nm for Phenylephrine hydrochloride (zero cross for Cetirizine hydrochloride) and 242.5 nm for Cetirizine hydrochloride (zero cross for Phenylephrine hydrochloride). These methods were validated as per ICH norms. Calibration curves were linear over the concentration ranges of 12-60 μg/ml for both drugs and for both methods. The validation study is statistically significant as all the statistical parameters are within the acceptance range (% RSD < 2.0 and S.D. < 2.0) for both accuracy and precision. The methods are successfully applied to pharmaceutical formulation, with no interference from excipients as indicated by the recovery study. The proposed methods are simple, rapid, economic and accurate for routine Simultaneous Estimation of Phenylephrine hydrochloride and Cetirizine hydrochloride. Simultaneous equation method was successfully applied to carry out dissolution study of commercial tablet formulation by using USP II dissolution test apparatus.
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Simultaneous Estimation of thiocolchicoside and aceclofenac in pharmaceutical dosage form by spectrophotometric and lc method
Der Pharmacia Lettre, 2010Co-Authors: Sohan S Chitlange, Pradeep S Shinde, Ganesh R Pawbake, Sagar B WankhedeAbstract:A simple, accurate, and reproducible UV spectrophotometric and HPLC method for Simultaneous Estimation of thiocolchicoside (THC) and aceclofenac (ACE) in combined tablet dosage form have been developed. The first developed method is Area under curve method, wavelength range selected are 264.5-254.5 nm for thiocolchicoside and 279.0-269.0 nm for aceclofenac respectively. Linearity was observed in concentration range of 4-36 μg/ml for thiocolchicoside as well as for aceclofenac. Second developed method is RP- HPLC method using Thermo C18 column (4.6 mm i.d. × 250 mm) and acetonitrile: water: 0.025M pot. dihydrogen orthophosphate buffer (pH adjusted to 3.0 with orthophosphoric acid) in the ratio of 70:10:20 % v/v/v as mobile phase. For HPLC method, linearity was observed in concentration range of 1-6 μg/ml for thiocolchicoside and 25-150 μg/ml for aceclofenac. Results of analysis were validated statistically and by recovery studies
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Simultaneous Estimation of amlodipine besilate and olmesartan medoxomil in pharmaceutical dosage form
Indian Journal of Pharmaceutical Sciences, 2009Co-Authors: Sagar B Wankhede, S B Wadkar, K C Raka, Sohan S ChitlangeAbstract:Two UV Spectrophotometric and one reverse phase high performance liquid chromatography methods have been developed for the Simultaneous Estimation of amlodipine besilate and olmesartan medoxomil in tablet dosage form. First UV spectrophotometric method was a determination using the Simultaneous equation method at 237.5 nm and 255.5 nm over the concentration range 10-50 μg/ml and 10-50 μg/ml, for amlodipine besilate and olmesartan medoxomil with accuracy 100.09%, and 100.22% respectively. Second UV spectrophotometric method was a determination using the area under curve method at 242.5-232.5 nm and 260.5-250.5 nm over the concentration range of 10-50 μg/ml and 10-50 μg/ml, for amlodipine besilate and olmesartan medoxomil with accuracy 100.10%, and 100.48%, respectively. In reverse phase high performance liquid chromatography analysis carried out using 0.05M potassuim dihydrogen phosphate buffer:acetonitrile (50:50 v/v) as the mobile phase and Kromasil C18 (4.6 mm i.d.Χ250 mm) column as the stationery phase with detection wavelength of 238 nm. Flow rate was 1.0 ml/min. Retention time for amlodipine besilate and olmesartan medoxomil were 3.69 and 5.36 min, respectively. Linearity was obtained in the concentration range of 4-20 μg/ml and 10-50 μg/ml for amlodipine besilate and olmesartan medoxomil, respectively. Proposed methods can be used for the Estimation of amlodipine besilate and olmesartan medoxomil in tablet dosage form provided all the validation parameters are met.
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stability indicating rp hplc method for Simultaneous Estimation of valsartan and amlodipine in capsule formulation
Asian Journal of Research in Chemistry, 2008Co-Authors: Sohan S Chitlange, Kiran Bagri, D M SakarkarAbstract:Present work describes a precise, accurate and reproducible Reverse phase High Performance Liquid Chromatographic (RP-HPLC) method for Simultaneous Estimation of Amlodipine besylate (AMLB) and Valsartan (VAT) on RP C-18 Column (Kromasil, 250 x 4.6 mm) using acetonitrile: phosphate buffer (0.02M, pH 3.0), (56:44 v/v) as mobile phase at a flow rate of 1.0 ml/min and the detection wavelength was 234 nm. The retention time for AMLB and VAT was found to be 3.07 and 6.20 min, respectively. The method was also applied for the determination of AMLB and VAT in the presence of their degradation products formed under variety of stress conditions. Proposed method was validated for precision, accuracy, linearity range, robustness and ruggedness.
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high performance thin layer chromatographic method for Simultaneous Estimation of ibuprofen and pseudoephedrine hydrochloride
Indian Journal of Pharmaceutical Sciences, 2008Co-Authors: Sohan S Chitlange, D M Sakarkar, Sagar B Wankhede, S G WadodkarAbstract:High performance thin layer chromatographic method is developed for Simultaneous Estimation of ibuprofen and pseudoephedrine hydrochloride in tablets. Silica gel 60F 254 plates were used as stationary phase and t.butanol: ethyl acetate: glacial acetic acid: water (7:4:2:2 v/v) as mobile phase. Wavelength selected for analysis was 254 nm. Percent Estimation of ibuprofen and pseudoephedrine hydrochloride was found to be 99.56% and 98.77%, respectively. Percent recovery for both the drugs was found in the range of 98.27% to 100.91%, respectively.
D M Sakarkar - One of the best experts on this subject based on the ideXlab platform.
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stability indicating rp hplc method for Simultaneous Estimation of valsartan and amlodipine in capsule formulation
Asian Journal of Research in Chemistry, 2008Co-Authors: Sohan S Chitlange, Kiran Bagri, D M SakarkarAbstract:Present work describes a precise, accurate and reproducible Reverse phase High Performance Liquid Chromatographic (RP-HPLC) method for Simultaneous Estimation of Amlodipine besylate (AMLB) and Valsartan (VAT) on RP C-18 Column (Kromasil, 250 x 4.6 mm) using acetonitrile: phosphate buffer (0.02M, pH 3.0), (56:44 v/v) as mobile phase at a flow rate of 1.0 ml/min and the detection wavelength was 234 nm. The retention time for AMLB and VAT was found to be 3.07 and 6.20 min, respectively. The method was also applied for the determination of AMLB and VAT in the presence of their degradation products formed under variety of stress conditions. Proposed method was validated for precision, accuracy, linearity range, robustness and ruggedness.
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high performance thin layer chromatographic method for Simultaneous Estimation of ibuprofen and pseudoephedrine hydrochloride
Indian Journal of Pharmaceutical Sciences, 2008Co-Authors: Sohan S Chitlange, D M Sakarkar, Sagar B Wankhede, S G WadodkarAbstract:High performance thin layer chromatographic method is developed for Simultaneous Estimation of ibuprofen and pseudoephedrine hydrochloride in tablets. Silica gel 60F 254 plates were used as stationary phase and t.butanol: ethyl acetate: glacial acetic acid: water (7:4:2:2 v/v) as mobile phase. Wavelength selected for analysis was 254 nm. Percent Estimation of ibuprofen and pseudoephedrine hydrochloride was found to be 99.56% and 98.77%, respectively. Percent recovery for both the drugs was found in the range of 98.27% to 100.91%, respectively.
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rp hplc method for Simultaneous Estimation of amlodipine and metoprolol in tablet formulation
Asian Journal of Pharmaceutics, 2008Co-Authors: Sohan S Chitlange, Mohammed Imran, D M SakarkarAbstract:Areverse phase high performance liquid chromatography (RP-HPLC) method for the Simultaneous Estimation of amlodipine and metoprolol in marketed formulation is developed. The determination was carried out on a Kromasil C18 (250 x 4.6 mm, 5 μm) column using a mobile phase of 0.02 M phosphate buffer solution: acetonitrile (70:30v/v, pH 3.0). The flow rate was 1.0ml/min with detection at 221 nm. The retention time for amlodipine was 2.57 min and for metoprolol 4.49 min. Amlodipine and metoprolol showed a linear response in the concentration range of 10-110 μg/ml. The correlation co-efficient (‘r’ value) for amlodipine and metoprolol was 0.9991 and 0.9992, respectively. The results of analysis have been validated statistically and by recovery studies. The percentage recoveries obtained for amlodipine and metoprolol ranges from 100.04 to 100.57%.
Kanaka M Durga - One of the best experts on this subject based on the ideXlab platform.
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development and validation of a stability indicating rp hplc method for Simultaneous Estimation of tapentadol and paracetamol in bulk and tablet dosage form
Asian Journal of Research in Chemistry, 2012Co-Authors: Thimma D Reddy, M Ramesh, Harishchandra R Babu, S Ramya, Kanaka M DurgaAbstract:A simple, selective, and sensitive stability-indicating Reverse phase high performance liquid chromatographic method has been developed and validated for the Simultaneous Estimation of Paracetamol and Tapentadol in bulk and tablet dosage form. The separation was achieved on a Thermo Hypersil BDS C18, 250 mm × 4.6 mm and 5 μm column with detection of 215nm at 40°c using an isocratic mobile phase mixture of Sodium di hydrogen ortho phosphate (0.1M, pH adjusted to 7 with ortho phosphoric acid) and methanol (60:40 v/v) at flow rate of 0.8 ml/min. The retention times for Paracetamol and Tapentadol were found to be 2.39min and 4.6min respectively with good resolution of 11.32. The method was validated for precision, linearity, accuracy, specificity, LOD, LOQ and Robustness. The calibration curves for Paracetamol and Tapentadol were linear in the concentration range of 325–975 μg/mL, and 50–150 μg/mL respectively. Recoveries for Paracetamol and Tapentadol were found to be in the range of 99% -100% and 100%-102% respectively. Forced degradation studies were performed using acid, base, water and hydrogen peroxide. No interference of the degradation products was detected. As the method is simple, accurate, precise the proposed method can be used for Simultaneous Estimation of these two drugs in their tablet dosage forms.
Cp Chan - One of the best experts on this subject based on the ideXlab platform.
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Simultaneous Estimation of an origin destination matrix and link choice proportions using traffic counts
Transportation Research Part A-policy and Practice, 2003Co-Authors: Cp ChanAbstract:Abstract A procedure for the Simultaneous Estimation of an origin–destination (OD) matrix and link choice proportions from OD survey data and traffic counts for congested network is proposed in this paper. Recognizing that link choice proportions in a network change with traffic conditions, and that the dispersion parameter of the route choice model should be updated for a current data set, this procedure performs statistical Estimation and traffic assignment alternately until convergence in order to obtain the best estimators for both the OD matrix and link choice proportions, which are consistent with the survey data and traffic counts. Results from a numerical study using a hypothetical network have shown that a model allowing θ to be estimated Simultaneously with an OD matrix from the observed data performs better than the model with a fixed predetermined θ . The application of the proposed model to the Tuen Mun Corridor network in Hong Kong is also presented in this paper. A reasonable estimate of the dispersion parameter θ for this network is obtained.
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Simultaneous Estimation of an origin destination matrix and link choice proportions using traffic counts
Transportation Research Part A-policy and Practice, 2003Co-Authors: Cp ChanAbstract:This paper proposes a procedure for the Simultaneous Estimation of an origin-destination (OD) matrix and link choice proportions from OD survey data and traffic counts for a congested network. Recognizing that link choice proportions in a network change with traffic conditions, and that the dispersion parameter of the route choice model should be updated for a current data set, this procedure performs statistical Estimation and traffic assignment alternatively until convergence in order to obtain the best estimates for both the OD matrix and link choice proportions, which are consistent with the survey data and traffic counts. Results from a numerical study using a hypothetical network show that a model allowing the dispersion parameter to be estimated Simultaneously with an OD matrix from the observed data performs better than the model with a fixed predetermined dispersion parameter. The proposed model also is applied to the Tuen Mun Corridor network in Hong Kong, and a reasonable estimate of the dispersion parameter for this network is obtained. The proposed algorithm seems quite robust towards inaccuracies in the survey data and in the number of observed links used.
A V Kasture - One of the best experts on this subject based on the ideXlab platform.
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international journal of pharmacy life sciences rp hplc Simultaneous Estimation of metronidazole and diloxanide furoate in combination
2010Co-Authors: K R Danao, R S Moon, A V Kasture, P G YeoleAbstract:A reverse phase high performance liquid chromatography method was developed for the Simultaneous Estimation of diloxanide furoate and metronidazole in formulation. The separation was achieved by octadecyl C8 column and a mixture of methanol: acetonitrite: 0.05M phosphate buffer at pH 4.0 (45:25:30 v/v) as eluent, at a flow rate of 1 ml/min. detection was carried out at 277 nm. Quantitation was done by external standard method. The retention time of metronidazole and diloxanide furoate was found to be 3.28 and 6.42 min, respectively. The method has validated for linearity, accuracy and precision. Linearity of metronidazole and diloxanide furoate were in the range of 5-50 µg/ml for both the drugs The mean recoveries obtained for metronidazole and diloxanide furoate were100.01% and 99.71%, respectively. The developed method was found to be accurate, precise, selective and rapid for the Simultaneous Estimation of metronidazole and diloxanide furoate in tablet.
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spectrophotometric methods for Simultaneous Estimation of rabeprazole and diclofenac from combined tablet dosage form
Asian Journal of Research in Chemistry, 2008Co-Authors: R W Lohe, A V Kasture, P B Suruse, M K Kale, P R Barethiya, S W LoheAbstract:Two simple, accurate, economical and reproducible spectrophotometric methods for Simultaneous Estimation of Rabeprazole and Diclofenac in combined tablet dosage form have been developed. The first developed method employs formation and solving of Simultaneous equation using two wavelengths 294.0 nm and 281.2 nm for formation of Simultaneous equation. Second developed method involves graphical absorbance ratio, using two wavelengths. Both of these methods obey Beer's law in the employed concentration ranges for respective methods. Results of analysis were validated statistically and by recovery studies.
