The Experts below are selected from a list of 216 Experts worldwide ranked by ideXlab platform
Makoto Komiyama - One of the best experts on this subject based on the ideXlab platform.
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Artificial site-selective DNA cutters to manipulate Single-Stranded DNA
Polymer Journal, 2012Co-Authors: Yuichiro Aiba, Makoto KomiyamaAbstract:Recent developments in artificial cutters for site-selective scission of Single-Stranded DNA are described. With the cutters composed of two oligonucleotide additives and Ce(IV)/EDTA, long Single-Stranded DNA can be selectively cut at target site and manipulated according to our needs. Recent progress regarding the development of artificial site-selective DNA cutters by chemical approaches are reviewed herein, with a special focus on site-selective cutters for Single-Stranded DNA. We employ a Ce(IV)/EDTA complex to serve as the catalyst, because it efficiently and selectively cuts Single-Stranded DNA. Using two complementary oligonucleotide additives, a gap structure is formed at the target site in the Single-Stranded DNA substrate. Owing to the substrate specificity of Ce(IV)/EDTA, the gap site is preferentially hydrolyzed, resulting in a site-selective DNA scission. The scission site is easily determined using the Watson–Crick base-pairing rule; thus, both the sequence and scission specificity can be tuned according to demand. The site-selective scission is greatly promoted by attaching a multiphosphonate to the termini of the oligonucleotide additives and placing this ligand at the gap site. The scission fragments can be connected with foreign DNA using ligase, and the recombinant DNA expresses the corresponding protein in E. coli . No undesired side reactions (for example, depurination, deletion, insertion, oxidative damage of nucleobases and off-target scission) occur throughout the DNA treatment.
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Artificial site-selective DNA cutters to manipulate Single-Stranded DNA
Polymer Journal, 2012Co-Authors: Yuichiro Aiba, Makoto KomiyamaAbstract:Recent developments in artificial cutters for site-selective scission of Single-Stranded DNA are described. With the cutters composed of two oligonucleotide additives and Ce(IV)/EDTA, long Single-Stranded DNA can be selectively cut at target site and manipulated according to our needs.
Yuichiro Aiba - One of the best experts on this subject based on the ideXlab platform.
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Artificial site-selective DNA cutters to manipulate Single-Stranded DNA
Polymer Journal, 2012Co-Authors: Yuichiro Aiba, Makoto KomiyamaAbstract:Recent developments in artificial cutters for site-selective scission of Single-Stranded DNA are described. With the cutters composed of two oligonucleotide additives and Ce(IV)/EDTA, long Single-Stranded DNA can be selectively cut at target site and manipulated according to our needs. Recent progress regarding the development of artificial site-selective DNA cutters by chemical approaches are reviewed herein, with a special focus on site-selective cutters for Single-Stranded DNA. We employ a Ce(IV)/EDTA complex to serve as the catalyst, because it efficiently and selectively cuts Single-Stranded DNA. Using two complementary oligonucleotide additives, a gap structure is formed at the target site in the Single-Stranded DNA substrate. Owing to the substrate specificity of Ce(IV)/EDTA, the gap site is preferentially hydrolyzed, resulting in a site-selective DNA scission. The scission site is easily determined using the Watson–Crick base-pairing rule; thus, both the sequence and scission specificity can be tuned according to demand. The site-selective scission is greatly promoted by attaching a multiphosphonate to the termini of the oligonucleotide additives and placing this ligand at the gap site. The scission fragments can be connected with foreign DNA using ligase, and the recombinant DNA expresses the corresponding protein in E. coli . No undesired side reactions (for example, depurination, deletion, insertion, oxidative damage of nucleobases and off-target scission) occur throughout the DNA treatment.
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Artificial site-selective DNA cutters to manipulate Single-Stranded DNA
Polymer Journal, 2012Co-Authors: Yuichiro Aiba, Makoto KomiyamaAbstract:Recent developments in artificial cutters for site-selective scission of Single-Stranded DNA are described. With the cutters composed of two oligonucleotide additives and Ce(IV)/EDTA, long Single-Stranded DNA can be selectively cut at target site and manipulated according to our needs.
Daniel T. Simmons - One of the best experts on this subject based on the ideXlab platform.
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The Origin DNA-Binding and Single-Stranded DNA-Binding Domains of Simian Virus 40 Large T Antigen Are Distinct
Journal of virology, 1998Co-Authors: Dianna Edgil, Daniel T. SimmonsAbstract:Little is known about the ability of simian virus 40 (SV40) T antigen to bind Single-Stranded DNA. We demonstrate here that a mutant (259-708) missing the first 258 amino acids of T antigen and its origin-binding domain bound Single-Stranded DNA at close to normal levels, whereas a mutant containing only the first 259 amino acids failed to bind any Single-Stranded DNA. The 259-708 mutant also assembled into high-molecular-weight oligomers in the presence of Single-Stranded DNA. Its ATPase activity was stimulated by Single-Stranded DNA similarly to the wild type (WT). Furthermore, WT T antigen’s ability to bind to Single-Stranded DNA was inhibited by the binding of two monoclonal antibodies that recognize a region after residue 362. These results show that the domain responsible for binding to Single-Stranded DNA is completely separate from the origin-binding domain.
G. Geoff Kneale - One of the best experts on this subject based on the ideXlab platform.
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Single Stranded DNA-binding proteins
Current Opinion in Structural Biology, 1992Co-Authors: G. Geoff KnealeAbstract:Abstract The past year has seen steady progress in our understanding of Single Stranded DNA-binding proteins and their interaction with DNA, most notably of the gene 5 proteins of bacteriophages fd and Pf, the gene 32 protein of bacteriophage T4 and the Escherichia coli SSB protein. A brief survey is given of recently identified Single Stranded DNA-binding proteins from both prokaryotic and eukaryotic sources, a number of which show pronounced sequence specificity. The crystal structure of the E. coli recA protein, which binds to both Single-Stranded and duplex DNA, has very recently been reported, and is briefly discussed.
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Single Stranded DNA-binding proteins
Current Opinion in Structural Biology, 1992Co-Authors: G. Geoff KnealeAbstract:Abstract The past year has seen steady progress in our understanding of Single Stranded DNA-binding proteins and their interaction with DNA, most notably of the gene 5 proteins of bacteriophages fd and Pf, the gene 32 protein of bacteriophage T4 and the Escherichia coli SSB protein. A brief survey is given of recently identified Single Stranded DNA-binding proteins from both prokaryotic and eukaryotic sources, a number of which show pronounced sequence specificity. The crystal structure of the E. coli recA protein, which binds to both Single-Stranded and duplex DNA, has very recently been reported, and is briefly discussed.
I. Davidson - One of the best experts on this subject based on the ideXlab platform.
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Viruses with Circular Single-Stranded DNA Genomes Are Everywhere!
Annual Review of Virology, 2017Co-Authors: L.m. Shulman, I. DavidsonAbstract:Circular Single-Stranded DNA viruses infect archaea, bacteria, and eukaryotic organisms. The relatively recent emergence of Single-Stranded DNA viruses, such as chicken anemia virus (CAV) and porcine circovirus 2 (PCV2), as serious pathogens of eukaryotes is due more to growing awareness than to the appearance of new pathogens or alteration of existing pathogens. In the case of the ubiquitous human circular Single-Stranded DNA virus family Anelloviridae, there is still no convincing direct causal relation to any specific disease. However, infections may play a role in autoimmunity by changing the homeostatic balance of proinflammatory cytokines and the human immune system, indirectly affecting the severity of diseases caused by other pathogens. Infections with CAV (family Anelloviridae, genus Gyrovirus) and PCV2 (family Circoviridae, genus Circovirus) are presented here because they are immunosuppressive and affect health in domesticated animals. CAV shares genomic organization, genomic orientation, and common features of major proteins with human anelloviruses, and PCV2 DNA may be present in human food and vaccines.
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Viruses with Circular Single-Stranded DNA Genomes Are Everywhere!
Annual Review of Virology, 2017Co-Authors: L.m. Shulman, I. DavidsonAbstract:Circular Single-Stranded DNA viruses infect archaea, bacteria, and eukaryotic organisms. The relatively recent emergence of Single-Stranded DNA viruses, such as chicken anemia virus (CAV) and porcine circovirus 2 (PCV2), as serious pathogens of eukaryotes is due more to growing awareness than to the appearance of new pathogens or alteration of existing pathogens. In the case of the ubiquitous human circular Single-Stranded DNA virus family Anelloviridae, there is still no convincing direct causal relation to any specific disease. However, infections may play a role in autoimmunity by changing the homeostatic balance of proinflammatory cytokines and the human immune system, indirectly affecting the severity of diseases caused by other pathogens. Infections with CAV (family Anelloviridae, genus Gyrovirus) and PCV2 (family Circoviridae, genus Circovirus) are presented here because they are immunosuppressive and affect health in domesticated animals. CAV shares genomic organization, genomic orientation, and c...
