The Experts below are selected from a list of 36759 Experts worldwide ranked by ideXlab platform
H. Schulte - One of the best experts on this subject based on the ideXlab platform.
-
plasma sitosterol elevations are associated with an increased incidence of coronary events in men results of a nested case control analysis of the prospective cardiovascular munster procam study
Nutrition Metabolism and Cardiovascular Diseases, 2006Co-Authors: G Assmann, Paul Cullen, John Ii R Erbey, Dena R Ramey, Frank Kannenberg, H. SchulteAbstract:BACKGROUND AND AIM: Sitosterolemia, a rare genetic disorder characterized by profoundly elevated plasma sitosterol concentrations, is associated with premature atherosclerosis in some individuals. This study was conducted to evaluate if the modest sitosterol elevations seen in the general population are also associated with the occurrence of coronary events. METHODS AND RESULTS: A nested case-control study using stored samples from male participants in the Prospective Cardiovascular Munster (PROCAM) study was performed. Each of 159 men who suffered a myocardial infarction or sudden coronary death (major coronary event) within 10 years of follow-up in PROCAM was matched with 2 controls (N = 318) by age, smoking status, and date of investigation. Analysis was performed using conditional logistic regression. Plasma sitosterol concentrations were elevated in cases compared with controls (4.94 +/- 3.44 micromol/L versus 4.27 +/- 2.38 micromol/L; P = 0.028). The upper quartile of sitosterol (>5.25 micromol/L) was associated with a 1.8-fold increase in risk (P or = 20% in 10 years as calculated using the PROCAM algorithm, high sitosterol concentrations were associated with an additional 3-fold increase in the incidence of coronary events (P = 0.032); a similar, significant relationship was observed between a high sitosterol/cholesterol ratio and coronary risk (P = 0.030). CONCLUSIONS: Elevations in sitosterol concentrations and the sitosterol/cholesterol ratio appear to be associated with an increased occurrence of major coronary events in men at high global risk of coronary heart disease. Further evaluations are warranted to confirm these preliminary findings.
-
plasma sitosterol elevations are associated with an increased incidence of coronary events in men results of a nested case control analysis of the prospective cardiovascular munster procam study
Nutrition Metabolism and Cardiovascular Diseases, 2006Co-Authors: G Assmann, Paul Cullen, John Ii R Erbey, Dena R Ramey, Frank Kannenberg, H. SchulteAbstract:Summary Background and aim Sitosterolemia, a rare genetic disorder characterized by profoundly elevated plasma sitosterol concentrations, is associated with premature atherosclerosis in some individuals. This study was conducted to evaluate if the modest sitosterol elevations seen in the general population are also associated with the occurrence of coronary events. Methods and results A nested case-control study using stored samples from male participants in the Prospective Cardiovascular Munster (PROCAM) study was performed. Each of 159 men who suffered a myocardial infarction or sudden coronary death (major coronary event) within 10 years of follow-up in PROCAM was matched with 2 controls ( N =318) by age, smoking status, and date of investigation. Analysis was performed using conditional logistic regression. Plasma sitosterol concentrations were elevated in cases compared with controls (4.94±3.44μmol/L versus 4.27±2.38μmol/L; P =0.028). The upper quartile of sitosterol (>5.25μmol/L) was associated with a 1.8-fold increase in risk ( P P =0.032); a similar, significant relationship was observed between a high sitosterol/cholesterol ratio and coronary risk ( P =0.030). Conclusions Elevations in sitosterol concentrations and the sitosterol/cholesterol ratio appear to be associated with an increased occurrence of major coronary events in men at high global risk of coronary heart disease. Further evaluations are warranted to confirm these preliminary findings.
G Assmann - One of the best experts on this subject based on the ideXlab platform.
-
plasma sitosterol elevations are associated with an increased incidence of coronary events in men results of a nested case control analysis of the prospective cardiovascular munster procam study
Nutrition Metabolism and Cardiovascular Diseases, 2006Co-Authors: G Assmann, Paul Cullen, John Ii R Erbey, Dena R Ramey, Frank Kannenberg, H. SchulteAbstract:BACKGROUND AND AIM: Sitosterolemia, a rare genetic disorder characterized by profoundly elevated plasma sitosterol concentrations, is associated with premature atherosclerosis in some individuals. This study was conducted to evaluate if the modest sitosterol elevations seen in the general population are also associated with the occurrence of coronary events. METHODS AND RESULTS: A nested case-control study using stored samples from male participants in the Prospective Cardiovascular Munster (PROCAM) study was performed. Each of 159 men who suffered a myocardial infarction or sudden coronary death (major coronary event) within 10 years of follow-up in PROCAM was matched with 2 controls (N = 318) by age, smoking status, and date of investigation. Analysis was performed using conditional logistic regression. Plasma sitosterol concentrations were elevated in cases compared with controls (4.94 +/- 3.44 micromol/L versus 4.27 +/- 2.38 micromol/L; P = 0.028). The upper quartile of sitosterol (>5.25 micromol/L) was associated with a 1.8-fold increase in risk (P or = 20% in 10 years as calculated using the PROCAM algorithm, high sitosterol concentrations were associated with an additional 3-fold increase in the incidence of coronary events (P = 0.032); a similar, significant relationship was observed between a high sitosterol/cholesterol ratio and coronary risk (P = 0.030). CONCLUSIONS: Elevations in sitosterol concentrations and the sitosterol/cholesterol ratio appear to be associated with an increased occurrence of major coronary events in men at high global risk of coronary heart disease. Further evaluations are warranted to confirm these preliminary findings.
-
plasma sitosterol elevations are associated with an increased incidence of coronary events in men results of a nested case control analysis of the prospective cardiovascular munster procam study
Nutrition Metabolism and Cardiovascular Diseases, 2006Co-Authors: G Assmann, Paul Cullen, John Ii R Erbey, Dena R Ramey, Frank Kannenberg, H. SchulteAbstract:Summary Background and aim Sitosterolemia, a rare genetic disorder characterized by profoundly elevated plasma sitosterol concentrations, is associated with premature atherosclerosis in some individuals. This study was conducted to evaluate if the modest sitosterol elevations seen in the general population are also associated with the occurrence of coronary events. Methods and results A nested case-control study using stored samples from male participants in the Prospective Cardiovascular Munster (PROCAM) study was performed. Each of 159 men who suffered a myocardial infarction or sudden coronary death (major coronary event) within 10 years of follow-up in PROCAM was matched with 2 controls ( N =318) by age, smoking status, and date of investigation. Analysis was performed using conditional logistic regression. Plasma sitosterol concentrations were elevated in cases compared with controls (4.94±3.44μmol/L versus 4.27±2.38μmol/L; P =0.028). The upper quartile of sitosterol (>5.25μmol/L) was associated with a 1.8-fold increase in risk ( P P =0.032); a similar, significant relationship was observed between a high sitosterol/cholesterol ratio and coronary risk ( P =0.030). Conclusions Elevations in sitosterol concentrations and the sitosterol/cholesterol ratio appear to be associated with an increased occurrence of major coronary events in men at high global risk of coronary heart disease. Further evaluations are warranted to confirm these preliminary findings.
Rajagopal Subramanyam - One of the best experts on this subject based on the ideXlab platform.
-
molecular dynamics simulation and binding studies of β sitosterol with human serum albumin and its biological relevance
Journal of Physical Chemistry B, 2010Co-Authors: Babu Sudhamalla, Mahesh Gokara, Navjeet Ahalawat, Damu G Amooru, Rajagopal SubramanyamAbstract:β-Sitosterol is a naturally occurring phytosterol that is widely used to cure atherosclerosis, diabetes, cancer, and inflammation and is also an antioxidant. Here, we studied the interaction of β-sitosterol, isolated from the aerial roots of Ficus bengalensis, with human serum albumin (HSA) at physiological pH 7.2 by using fluorescence, circular dichroism (CD), molecular docking, and molecular dynamics simulation methods. The experimental results show that the intrinsic fluorescence of HSA is quenched by addition of β-sitosterol through a static quenching mechanism. The binding constant of the compound to HSA, calculated from fluorescence data, was found to be Kβ-sitosterol = 4.6 ± 0.01 × 103 M−1, which corresponds to −5.0 kcal M−1 of free energy. Upon binding of β-sitosterol to HSA, the protein secondary structure was partially unfolded. Specifically, the molecular dynamics study makes an important contribution to understanding the effect of the binding of β-sitosterol on conformational changes of HSA an...
-
molecular dynamics simulation and binding studies of β sitosterol with human serum albumin and its biological relevance
Journal of Physical Chemistry B, 2010Co-Authors: Babu Sudhamalla, Mahesh Gokara, Navjeet Ahalawat, Damu G Amooru, Rajagopal SubramanyamAbstract:Beta-sitosterol is a naturally occurring phytosterol that is widely used to cure atherosclerosis, diabetes, cancer, and inflammation and is also an antioxidant. Here, we studied the interaction of beta-sitosterol, isolated from the aerial roots of Ficus bengalensis, with human serum albumin (HSA) at physiological pH 7.2 by using fluorescence, circular dichroism (CD), molecular docking, and molecular dynamics simulation methods. The experimental results show that the intrinsic fluorescence of HSA is quenched by addition of beta-sitosterol through a static quenching mechanism. The binding constant of the compound to HSA, calculated from fluorescence data, was found to be K(beta-sitosterol) = 4.6 +/- 0.01 x 10(3) M(-1), which corresponds to -5.0 kcal M(-1) of free energy. Upon binding of beta-sitosterol to HSA, the protein secondary structure was partially unfolded. Specifically, the molecular dynamics study makes an important contribution to understanding the effect of the binding of beta-sitosterol on conformational changes of HSA and the stability of a protein-drug complex system in aqueous solution. Molecular docking studies revealed that the beta-sitosterol can bind in the large hydrophobic cavity of subdomain IIA, mainly by the hydrophobic interaction but also by hydrogen bond interactions between the hydroxyl (OH) group of carbon-3 of beta-sitosterol to Arg(257), Ser(287), and Ala(261) of HSA, with hydrogen bond distances of 1.9, 2.4, and 2.2 A, respectively.
Navjeet Ahalawat - One of the best experts on this subject based on the ideXlab platform.
-
molecular dynamics simulation and binding studies of β sitosterol with human serum albumin and its biological relevance
Journal of Physical Chemistry B, 2010Co-Authors: Babu Sudhamalla, Mahesh Gokara, Navjeet Ahalawat, Damu G Amooru, Rajagopal SubramanyamAbstract:β-Sitosterol is a naturally occurring phytosterol that is widely used to cure atherosclerosis, diabetes, cancer, and inflammation and is also an antioxidant. Here, we studied the interaction of β-sitosterol, isolated from the aerial roots of Ficus bengalensis, with human serum albumin (HSA) at physiological pH 7.2 by using fluorescence, circular dichroism (CD), molecular docking, and molecular dynamics simulation methods. The experimental results show that the intrinsic fluorescence of HSA is quenched by addition of β-sitosterol through a static quenching mechanism. The binding constant of the compound to HSA, calculated from fluorescence data, was found to be Kβ-sitosterol = 4.6 ± 0.01 × 103 M−1, which corresponds to −5.0 kcal M−1 of free energy. Upon binding of β-sitosterol to HSA, the protein secondary structure was partially unfolded. Specifically, the molecular dynamics study makes an important contribution to understanding the effect of the binding of β-sitosterol on conformational changes of HSA an...
-
molecular dynamics simulation and binding studies of β sitosterol with human serum albumin and its biological relevance
Journal of Physical Chemistry B, 2010Co-Authors: Babu Sudhamalla, Mahesh Gokara, Navjeet Ahalawat, Damu G Amooru, Rajagopal SubramanyamAbstract:Beta-sitosterol is a naturally occurring phytosterol that is widely used to cure atherosclerosis, diabetes, cancer, and inflammation and is also an antioxidant. Here, we studied the interaction of beta-sitosterol, isolated from the aerial roots of Ficus bengalensis, with human serum albumin (HSA) at physiological pH 7.2 by using fluorescence, circular dichroism (CD), molecular docking, and molecular dynamics simulation methods. The experimental results show that the intrinsic fluorescence of HSA is quenched by addition of beta-sitosterol through a static quenching mechanism. The binding constant of the compound to HSA, calculated from fluorescence data, was found to be K(beta-sitosterol) = 4.6 +/- 0.01 x 10(3) M(-1), which corresponds to -5.0 kcal M(-1) of free energy. Upon binding of beta-sitosterol to HSA, the protein secondary structure was partially unfolded. Specifically, the molecular dynamics study makes an important contribution to understanding the effect of the binding of beta-sitosterol on conformational changes of HSA and the stability of a protein-drug complex system in aqueous solution. Molecular docking studies revealed that the beta-sitosterol can bind in the large hydrophobic cavity of subdomain IIA, mainly by the hydrophobic interaction but also by hydrogen bond interactions between the hydroxyl (OH) group of carbon-3 of beta-sitosterol to Arg(257), Ser(287), and Ala(261) of HSA, with hydrogen bond distances of 1.9, 2.4, and 2.2 A, respectively.
Frank Kannenberg - One of the best experts on this subject based on the ideXlab platform.
-
plasma sitosterol elevations are associated with an increased incidence of coronary events in men results of a nested case control analysis of the prospective cardiovascular munster procam study
Nutrition Metabolism and Cardiovascular Diseases, 2006Co-Authors: G Assmann, Paul Cullen, John Ii R Erbey, Dena R Ramey, Frank Kannenberg, H. SchulteAbstract:BACKGROUND AND AIM: Sitosterolemia, a rare genetic disorder characterized by profoundly elevated plasma sitosterol concentrations, is associated with premature atherosclerosis in some individuals. This study was conducted to evaluate if the modest sitosterol elevations seen in the general population are also associated with the occurrence of coronary events. METHODS AND RESULTS: A nested case-control study using stored samples from male participants in the Prospective Cardiovascular Munster (PROCAM) study was performed. Each of 159 men who suffered a myocardial infarction or sudden coronary death (major coronary event) within 10 years of follow-up in PROCAM was matched with 2 controls (N = 318) by age, smoking status, and date of investigation. Analysis was performed using conditional logistic regression. Plasma sitosterol concentrations were elevated in cases compared with controls (4.94 +/- 3.44 micromol/L versus 4.27 +/- 2.38 micromol/L; P = 0.028). The upper quartile of sitosterol (>5.25 micromol/L) was associated with a 1.8-fold increase in risk (P or = 20% in 10 years as calculated using the PROCAM algorithm, high sitosterol concentrations were associated with an additional 3-fold increase in the incidence of coronary events (P = 0.032); a similar, significant relationship was observed between a high sitosterol/cholesterol ratio and coronary risk (P = 0.030). CONCLUSIONS: Elevations in sitosterol concentrations and the sitosterol/cholesterol ratio appear to be associated with an increased occurrence of major coronary events in men at high global risk of coronary heart disease. Further evaluations are warranted to confirm these preliminary findings.
-
plasma sitosterol elevations are associated with an increased incidence of coronary events in men results of a nested case control analysis of the prospective cardiovascular munster procam study
Nutrition Metabolism and Cardiovascular Diseases, 2006Co-Authors: G Assmann, Paul Cullen, John Ii R Erbey, Dena R Ramey, Frank Kannenberg, H. SchulteAbstract:Summary Background and aim Sitosterolemia, a rare genetic disorder characterized by profoundly elevated plasma sitosterol concentrations, is associated with premature atherosclerosis in some individuals. This study was conducted to evaluate if the modest sitosterol elevations seen in the general population are also associated with the occurrence of coronary events. Methods and results A nested case-control study using stored samples from male participants in the Prospective Cardiovascular Munster (PROCAM) study was performed. Each of 159 men who suffered a myocardial infarction or sudden coronary death (major coronary event) within 10 years of follow-up in PROCAM was matched with 2 controls ( N =318) by age, smoking status, and date of investigation. Analysis was performed using conditional logistic regression. Plasma sitosterol concentrations were elevated in cases compared with controls (4.94±3.44μmol/L versus 4.27±2.38μmol/L; P =0.028). The upper quartile of sitosterol (>5.25μmol/L) was associated with a 1.8-fold increase in risk ( P P =0.032); a similar, significant relationship was observed between a high sitosterol/cholesterol ratio and coronary risk ( P =0.030). Conclusions Elevations in sitosterol concentrations and the sitosterol/cholesterol ratio appear to be associated with an increased occurrence of major coronary events in men at high global risk of coronary heart disease. Further evaluations are warranted to confirm these preliminary findings.