The Experts below are selected from a list of 312 Experts worldwide ranked by ideXlab platform
Michael R Hamblin - One of the best experts on this subject based on the ideXlab platform.
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antimicrobial blue light inactivation of pseudomonas aeruginosa by photo excitation of endogenous porphyrins in vitro and in vivo studies
Lasers in Surgery and Medicine, 2016Co-Authors: Rehab M Amin, Michael R Hamblin, Brijesh Bhayana, Tianhong DaiAbstract:Pseudomonas aeruginosa is among the most common pathogens that cause nosocomial infections and is responsible for about 10% of all hospital-acquired infections. In the present study, we investigated the potential development of tolerance of P. aeruginosa to antimicrobial blue light by carrying 10 successive cycles of sublethal blue light inactivation. The high-performance liquid chromatographic (HPLC) analysis was performed to identify endogenous porphyrins in P. aeruginosa cells. In addition, we tested the effectiveness of antimicrobial blue light in a mouse model of nonlethal Skin Abrasion infection by using a bioluminescent strain of P. aeruginosa. The results demonstrated that no tolerance was developed to antimicrobial blue light in P. aeruginosa after 10 cycles of sub-lethal inactivation. HPLC analysis showed that P. aeruginosa is capable of producing endogenous porphyrins in particularly, coproporphyrin III, which are assumed to be responsible for the photodynamic effects of blue light alone. P. aeruginosa infection was eradicated by antimicrobial blue light alone (48 J/cm(2) ) without any added photosensitizer molecules in the mouse model. In conclusion, endogenous photosensitization using blue light should gain considerable attention as an effective and safe alternative antimicrobial therapy for Skin infections. Lasers Surg. Med. 48:562-568, 2016. © 2016 Wiley Periodicals, Inc.
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antimicrobial photodynamic therapy with rlp068 kills methicillin resistant staphylococcus aureus and improves wound healing in a mouse model of infected Skin Abrasion pdt with rlp068 cl in infected mouse Skin Abrasion
Journal of Biophotonics, 2013Co-Authors: Daniela Vecchio, Tianhong Dai, Michael R Hamblin, Liyi Huang, Lia Fantetti, Gabrio RoncucciAbstract:Photodynamic therapy (PDT) is an alternative treatment for infections that can kill drug resistant bacteria without damaging host-tissue. In this study we used bioluminescent methicillin-resistant Staphylococcus aureus, in a mouse Skin Abrasion model, to investigate the effect of PDT on bacterial inactivation and wound healing. RLP068/Cl, a tetracationic Zn(II)phthalocyanine derivative and toluidine blue (TBO) were used. The light-dose response of PDT to kill bacteria in vivo and the possible recurrence in the days post-treatment were monitored by real-time bioluminescence imaging, and wound healing by digital photography. The results showed PDT with RLP068/Cl (but not TBO) was able to kill bacteria, to inhibit bacterial re-growth after the treatment and to significantly accelerate the wound healing process (© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim).
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Blue light eliminates community-acquired methicillin-resistant Staphylococcus aureus in infected mouse Skin Abrasions.
Photomedicine and laser surgery, 2013Co-Authors: Tianhong Dai, Asheesh Gupta, Ying-ying Huang, Margaret E. Sherwood, Clinton K. Murray, Mark S. Vrahas, Tammy L Kielian, Michael R HamblinAbstract:Abstract Background and objective: Bacterial Skin and soft tissue infections (SSTI) affect millions of individuals annually in the United States. Treatment of SSTI has been significantly complicated by the increasing emergence of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) strains. The objective of this study was to demonstrate the efficacy of blue light (415±10 nm) therapy for eliminating CA-MRSA infections in Skin Abrasions of mice. Methods: The susceptibilities of a CA-MRSA strain (USA300LAC) and human keratinocytes (HaCaT) to blue light inactivation were compared by in vitro culture studies. A mouse model of Skin Abrasion infection was developed using bioluminescent USA300LAC::lux. Blue light was delivered to the infected mouse Skin Abrasions at 30 min (acute) and 24 h (established) after the bacterial inoculation. Bioluminescence imaging was used to monitor in real time the extent of infection in mice. Results: USA300LAC was much more susceptible to blue light inactivatio...
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Blue Dye and Red Light, a Dynamic Combination for Prophylaxis and Treatment of Cutaneous Candida albicans Infections in Mice
Antimicrobial agents and chemotherapy, 2011Co-Authors: Tianhong Dai, George P Tegos, Vida J. Bil De Arce, Michael R HamblinAbstract:The objective of this study was to investigate photodynamic therapy (PDT), using blue dye and red light, for prophylaxis and treatment of cutaneous Candida albicans infections in mice. A mouse model of Skin Abrasion infected with C. albicans was developed by inoculating wounds measuring 1.2 cm by 1.2 cm with 10(6) or 10(7) CFU. The use of a luciferase-expressing strain of C. albicans allowed real-time monitoring of the extent of infection in mice noninvasively through bioluminescence imaging. The phenothiazinium salts toluidine blue O (TBO), methylene blue (MB), and new methylene blue (NMB) were compared as photosensitizers (PS) for the photodynamic inactivation of C. albicans in vitro. PDT in vivo was initiated either at 30 min or at 24 h after fungal inoculation to investigate the efficacies of PDT for both prophylaxis and treatment of infections. Light at 635 ± 15 nm or 660 ± 15 nm was delivered with a light dose of 78 J/cm(2) (for PDT at 30 min postinfection) or 120 J/cm(2) (for PDT at 24 h postinfection) in multiple exposures with bioluminescence imaging taking place after each exposure of light. In vitro studies showed that NMB was superior to TBO and MB as the PS in the photodynamic inactivation of C. albicans. The efficacy of PDT was related to the ratio of PS concentration to fungal cell density. PDT in vivo initiated either at 30 min or at 24 h postinfection significantly reduced C. albicans burden in the infected mouse Skin Abrasion wounds. These data suggest that PDT is a viable approach for prophylaxis and treatment of cutaneous C. albicans infections.
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photodynamic therapy for methicillin resistant staphylococcus aureus infection in a mouse Skin Abrasion model
Lasers in Surgery and Medicine, 2010Co-Authors: Tianhong Dai, George P Tegos, Timur Zhiyentayev, Eleftherios Mylonakis, Michael R HamblinAbstract:Background and Objective Methicillin-resistant Staphylococcus aureus (MRSA) Skin infections are now known to be a common and important problem in the Unites States. The objective of this study was to investigate the efficacy of photodynamic therapy (PDT) for the treatment of MRSA infection in Skin Abrasion wounds using a mouse model. Study Design/Materials and Methods A mouse model of Skin Abrasion wound infected with MRSA was developed. Bioluminescent strain of MRSA, a derivative of ATCC 33591, was used to allow the real-time monitoring of the extent of infection in mouse wounds. PDT was performed with the combination of a polyethylenimine (PEI)–ce6 photosensitizer (PS) and non-coherent red light. In vivo fluorescence imaging was carried out to evaluate the effect of photobleaching of PS during PDT. Results In vivo fluorescence imaging of conjugate PEI–ce6 applied in mice indicated the photobleaching effect of the PS during PDT. PDT induced on average 2.7 log10 of inactivation of MRSA as judged by loss of bioluminescence in mouse Skin Abrasion wounds and accelerated the wound healing on average by 8.6 days in comparison to the untreated infected wounds. Photobleaching of PS in the wound was overcome by adding the PS solution in aliquots. Conclusion PDT may represent an alternative approach for the treatment of MRSA Skin infections. Lasers Surg. Med. 42:38–44, 2010. © 2010 Wiley-Liss, Inc.
Tianhong Dai - One of the best experts on this subject based on the ideXlab platform.
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quinine enhances photo inactivation of gram negative bacteria
The Journal of Infectious Diseases, 2020Co-Authors: Leon G Leanse, Puting Dong, Xueping S Goh, Jixin Cheng, David C Hooper, Tianhong DaiAbstract:BACKGROUND Antimicrobial resistance is a significant concern to public health, and there is a pressing need to develop novel antimicrobial therapeutic modalities. METHODS In this study, we investigated the capacity for quinine hydrochloride (Q-HCL) to enhance the antimicrobial effects of antimicrobial blue light ([aBL] 405 nm wavelength) against multidrug-resistant (MDR) Gram-negative bacteria in vitro and in vivo. RESULTS Our findings demonstrated the significant improvement in the inactivation of MDR Pseudomonas aeruginosa and Acinetobacter baumannii (planktonic cells and biofilms) when aBL was illuminated during Q-HCL exposure. Furthermore, the addition of Q-HCL significantly potentiated the antimicrobial effects of aBL in a mouse Skin Abrasion infection model. In addition, combined exposure of aBL and Q-HCL did not result in any significant apoptosis when exposed to uninfected mouse Skin. CONCLUSIONS In conclusion, aBL in combination with Q-HCL may offer a novel approach for the treatment of infections caused by MDR bacteria.
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antimicrobial blue light inactivation of pseudomonas aeruginosa by photo excitation of endogenous porphyrins in vitro and in vivo studies
Lasers in Surgery and Medicine, 2016Co-Authors: Rehab M Amin, Michael R Hamblin, Brijesh Bhayana, Tianhong DaiAbstract:Pseudomonas aeruginosa is among the most common pathogens that cause nosocomial infections and is responsible for about 10% of all hospital-acquired infections. In the present study, we investigated the potential development of tolerance of P. aeruginosa to antimicrobial blue light by carrying 10 successive cycles of sublethal blue light inactivation. The high-performance liquid chromatographic (HPLC) analysis was performed to identify endogenous porphyrins in P. aeruginosa cells. In addition, we tested the effectiveness of antimicrobial blue light in a mouse model of nonlethal Skin Abrasion infection by using a bioluminescent strain of P. aeruginosa. The results demonstrated that no tolerance was developed to antimicrobial blue light in P. aeruginosa after 10 cycles of sub-lethal inactivation. HPLC analysis showed that P. aeruginosa is capable of producing endogenous porphyrins in particularly, coproporphyrin III, which are assumed to be responsible for the photodynamic effects of blue light alone. P. aeruginosa infection was eradicated by antimicrobial blue light alone (48 J/cm(2) ) without any added photosensitizer molecules in the mouse model. In conclusion, endogenous photosensitization using blue light should gain considerable attention as an effective and safe alternative antimicrobial therapy for Skin infections. Lasers Surg. Med. 48:562-568, 2016. © 2016 Wiley Periodicals, Inc.
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antimicrobial photodynamic therapy with rlp068 kills methicillin resistant staphylococcus aureus and improves wound healing in a mouse model of infected Skin Abrasion pdt with rlp068 cl in infected mouse Skin Abrasion
Journal of Biophotonics, 2013Co-Authors: Daniela Vecchio, Tianhong Dai, Michael R Hamblin, Liyi Huang, Lia Fantetti, Gabrio RoncucciAbstract:Photodynamic therapy (PDT) is an alternative treatment for infections that can kill drug resistant bacteria without damaging host-tissue. In this study we used bioluminescent methicillin-resistant Staphylococcus aureus, in a mouse Skin Abrasion model, to investigate the effect of PDT on bacterial inactivation and wound healing. RLP068/Cl, a tetracationic Zn(II)phthalocyanine derivative and toluidine blue (TBO) were used. The light-dose response of PDT to kill bacteria in vivo and the possible recurrence in the days post-treatment were monitored by real-time bioluminescence imaging, and wound healing by digital photography. The results showed PDT with RLP068/Cl (but not TBO) was able to kill bacteria, to inhibit bacterial re-growth after the treatment and to significantly accelerate the wound healing process (© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim).
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antimicrobial photodynamic therapy with rlp068 kills methicillin resistant staphylococcus aureus and improves wound healing in a mouse model of infected Skin Abrasion pdt with rlp068 cl in infected mouse Skin Abrasion
Journal of Biophotonics, 2013Co-Authors: Daniela Vecchio, Tianhong Dai, Liyi Huang, Lia Fantetti, Gabrio Roncucci, Michael R HambliAbstract:Photodynamic therapy (PDT) is an alternative treatment for infections that can kill drug resistant bacteria without damaging host-tissue. In this study we used bioluminescent methicillin-resistant Staphylococcus aureus, in a mouse Skin Abrasion model, to investigate the effect of PDT on bacterial inactivation and wound healing. RLP068/Cl, a tetracationic Zn(II)phthalocyanine derivative and toluidine blue (TBO) were used. The light-dose response of PDT to kill bacteria in vivo and the possible recurrence in the days post-treatment were monitored by real-time bioluminescence imaging, and wound healing by digital photography. The results showed PDT with RLP068/Cl (but not TBO) was able to kill bacteria, to inhibit bacterial re-growth after the treatment and to significantly accelerate the wound healing process. Successive bioluminescence images of a representative mouse Skin scratch model infected with 108 CFU MRSA.
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Blue light eliminates community-acquired methicillin-resistant Staphylococcus aureus in infected mouse Skin Abrasions.
Photomedicine and laser surgery, 2013Co-Authors: Tianhong Dai, Asheesh Gupta, Ying-ying Huang, Margaret E. Sherwood, Clinton K. Murray, Mark S. Vrahas, Tammy L Kielian, Michael R HamblinAbstract:Abstract Background and objective: Bacterial Skin and soft tissue infections (SSTI) affect millions of individuals annually in the United States. Treatment of SSTI has been significantly complicated by the increasing emergence of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) strains. The objective of this study was to demonstrate the efficacy of blue light (415±10 nm) therapy for eliminating CA-MRSA infections in Skin Abrasions of mice. Methods: The susceptibilities of a CA-MRSA strain (USA300LAC) and human keratinocytes (HaCaT) to blue light inactivation were compared by in vitro culture studies. A mouse model of Skin Abrasion infection was developed using bioluminescent USA300LAC::lux. Blue light was delivered to the infected mouse Skin Abrasions at 30 min (acute) and 24 h (established) after the bacterial inoculation. Bioluminescence imaging was used to monitor in real time the extent of infection in mice. Results: USA300LAC was much more susceptible to blue light inactivatio...
Michael R Hambli - One of the best experts on this subject based on the ideXlab platform.
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antimicrobial photodynamic therapy with rlp068 kills methicillin resistant staphylococcus aureus and improves wound healing in a mouse model of infected Skin Abrasion pdt with rlp068 cl in infected mouse Skin Abrasion
Journal of Biophotonics, 2013Co-Authors: Daniela Vecchio, Tianhong Dai, Liyi Huang, Lia Fantetti, Gabrio Roncucci, Michael R HambliAbstract:Photodynamic therapy (PDT) is an alternative treatment for infections that can kill drug resistant bacteria without damaging host-tissue. In this study we used bioluminescent methicillin-resistant Staphylococcus aureus, in a mouse Skin Abrasion model, to investigate the effect of PDT on bacterial inactivation and wound healing. RLP068/Cl, a tetracationic Zn(II)phthalocyanine derivative and toluidine blue (TBO) were used. The light-dose response of PDT to kill bacteria in vivo and the possible recurrence in the days post-treatment were monitored by real-time bioluminescence imaging, and wound healing by digital photography. The results showed PDT with RLP068/Cl (but not TBO) was able to kill bacteria, to inhibit bacterial re-growth after the treatment and to significantly accelerate the wound healing process. Successive bioluminescence images of a representative mouse Skin scratch model infected with 108 CFU MRSA.
Liyi Huang - One of the best experts on this subject based on the ideXlab platform.
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antimicrobial photodynamic therapy with rlp068 kills methicillin resistant staphylococcus aureus and improves wound healing in a mouse model of infected Skin Abrasion pdt with rlp068 cl in infected mouse Skin Abrasion
Journal of Biophotonics, 2013Co-Authors: Daniela Vecchio, Tianhong Dai, Michael R Hamblin, Liyi Huang, Lia Fantetti, Gabrio RoncucciAbstract:Photodynamic therapy (PDT) is an alternative treatment for infections that can kill drug resistant bacteria without damaging host-tissue. In this study we used bioluminescent methicillin-resistant Staphylococcus aureus, in a mouse Skin Abrasion model, to investigate the effect of PDT on bacterial inactivation and wound healing. RLP068/Cl, a tetracationic Zn(II)phthalocyanine derivative and toluidine blue (TBO) were used. The light-dose response of PDT to kill bacteria in vivo and the possible recurrence in the days post-treatment were monitored by real-time bioluminescence imaging, and wound healing by digital photography. The results showed PDT with RLP068/Cl (but not TBO) was able to kill bacteria, to inhibit bacterial re-growth after the treatment and to significantly accelerate the wound healing process (© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim).
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antimicrobial photodynamic therapy with rlp068 kills methicillin resistant staphylococcus aureus and improves wound healing in a mouse model of infected Skin Abrasion pdt with rlp068 cl in infected mouse Skin Abrasion
Journal of Biophotonics, 2013Co-Authors: Daniela Vecchio, Tianhong Dai, Liyi Huang, Lia Fantetti, Gabrio Roncucci, Michael R HambliAbstract:Photodynamic therapy (PDT) is an alternative treatment for infections that can kill drug resistant bacteria without damaging host-tissue. In this study we used bioluminescent methicillin-resistant Staphylococcus aureus, in a mouse Skin Abrasion model, to investigate the effect of PDT on bacterial inactivation and wound healing. RLP068/Cl, a tetracationic Zn(II)phthalocyanine derivative and toluidine blue (TBO) were used. The light-dose response of PDT to kill bacteria in vivo and the possible recurrence in the days post-treatment were monitored by real-time bioluminescence imaging, and wound healing by digital photography. The results showed PDT with RLP068/Cl (but not TBO) was able to kill bacteria, to inhibit bacterial re-growth after the treatment and to significantly accelerate the wound healing process. Successive bioluminescence images of a representative mouse Skin scratch model infected with 108 CFU MRSA.
Gérald Quatrehomme - One of the best experts on this subject based on the ideXlab platform.
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Suicidal hanging resulting in decapitation: A case report and review of the literature.
Forensic science international, 2017Co-Authors: Céline Leccia, Véronique Alunni, Gérald QuatrehommeAbstract:Decapitation following suicidal hanging is rarely encountered in forensic practice. The authors report a case of suicidal hanging resulting in decapitation following a fall of 5m. This case is compared with 30 cases found in the literature. Several factors including type of rope, Skin Abrasion, level of the severed vertebrae, thyroid cartilage and hyoid bone injuries and vital signs are studied. The force applied to the neck and the kinetic energy were calculated. The kinetic energy (ranging from 1820 to 7310J) takes into account the weight of the victim but also the length of the rope (height of the fall). The speed of the body as it is stopped by the rope ranged between 6.49 and 14.01ms-1.
