The Experts below are selected from a list of 45 Experts worldwide ranked by ideXlab platform
Nicolas H Voelcker - One of the best experts on this subject based on the ideXlab platform.
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multiparameter toxicity screening on a chip effects of uv radiation and titanium dioxide nanoparticles on hacat Cells
Biomicrofluidics, 2019Co-Authors: Scott Mccormick, Louise E Smith, Amy Holmes, Ziqiu Tong, Enzo Lombi, Nicolas H Voelcker, Craig PriestAbstract:Microfluidic screening is gaining attention as an efficient method for evaluating nanomaterial toxicity. Here, we consider a multiparameter treatment where nanomaterials interact with Cells in the presence of a secondary exposure (UV radiation). The microfluidic device contains channels that permit immobilization of HaCaT Cells (human Skin Cell Line), delivery of titanium dioxide nanoparticles (TNPs), and exposure to a known dose of UV radiation. The effect of single-parameter exposures (UV or TNP) was first studied as a benchmark, and then multiparameter toxicity (UV and TNP) at different concentrations was explored. The results demonstrate a concentration-dependent protective effect of TNP when exposed to UV irradiation.
Scott Mccormick - One of the best experts on this subject based on the ideXlab platform.
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multiparameter toxicity screening on a chip effects of uv radiation and titanium dioxide nanoparticles on hacat Cells
Biomicrofluidics, 2019Co-Authors: Scott Mccormick, Louise E Smith, Amy Holmes, Ziqiu Tong, Enzo Lombi, Nicolas H Voelcker, Craig PriestAbstract:Microfluidic screening is gaining attention as an efficient method for evaluating nanomaterial toxicity. Here, we consider a multiparameter treatment where nanomaterials interact with Cells in the presence of a secondary exposure (UV radiation). The microfluidic device contains channels that permit immobilization of HaCaT Cells (human Skin Cell Line), delivery of titanium dioxide nanoparticles (TNPs), and exposure to a known dose of UV radiation. The effect of single-parameter exposures (UV or TNP) was first studied as a benchmark, and then multiparameter toxicity (UV and TNP) at different concentrations was explored. The results demonstrate a concentration-dependent protective effect of TNP when exposed to UV irradiation.
Vahideh Imeni - One of the best experts on this subject based on the ideXlab platform.
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hybrid benzoxazole coumarin compounds induce death receptor mediated switchable apoptotic and necroptotic Cell death on hn 5 head and neck cancer Cell Line
Anti-cancer Agents in Medicinal Chemistry, 2017Co-Authors: Sahar Yaghmaei, Parichehr Ghalayani, Siamak Salami, Farahnaz Nourmohammadian, Soheila Koohestanimobarhan, Vahideh ImeniAbstract:Background: Efficacy of multimodality approaches for the treatment of squamous Cell cancer of the head and neck has remained unsatisfactory and further advances are critically required. Targeted Cell death induction is a novel therapeutic approach that may help to improve clinical management of Head and Neck cancer patients. Objective: The potency of novel hybrid benzoxazole-coumarins on the induction of apoptotic and/or necroptotic Cell death were evaluated in a Head and Neck carcinoma Cell Line, HN-5, and a human Skin Cell Line, AGO1522. Methods: Quantitative toxicity of the synthesized compounds were elucidated by MTT assay, the specific activity of caspase-3 and -9 were measured by the colorimetric method and zVAD was used to block apoptosis. Expression of Cell death related genes were studied using quantitative PCR. Results: All three compounds were revealed IC50 value around 51.96±7.15 microM in HN-5 Cells which were significantly lower than observed IC50 for AGO1522, 121.93±3.66 microM (p=0.001). Significant increase expression of FAS, FASL and TRIAL were observed in the treated Cells with or without pretreatment with zVAD. In the absence of pretreatment, treatment lead to the induction of apoptosis with a significant increase in caspase-3 gene expression and caspase-3 activity without a significant increase in expression or activity of caspase-9 and other components of the intrinsic apoptotic pathway. However, in the zVAD pretreated Cells, necroptotic Cell death with a significant increase in expression of RIP1, RIP3, and MLKL genes was observed Conclusion: The novel hybrid benzoxazole-coumarins effectively induce Caspase-3 dependent apoptosis in HN-5 cancer Cells, but also could circumvent the blockage of apoptotic Cell death by induction of necroptosis.
Qi-ya Zhang - One of the best experts on this subject based on the ideXlab platform.
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development of an ussuri catfish pseudobagrus ussuriensis Skin Cell Line displaying differential cytopathic effects to three aquatic animal viruses
Virus Research, 2014Co-Authors: Xiaoying Lei, Fengjian Zhou, Qi-ya ZhangAbstract:An Ussuri catfish Pseudobagrus ussuriensis Skin (UCS) Cell Line was developed and subcultured for more than 60 passages. UCS Cells consisted of mostly epithelial-like Cells and multiplied well in TC199 medium supplemented with 10% fetal bovine serum at 25 degrees C. Chromosome analysis revealed that most UCS Cells had a normal diploid karyotype with 2n = 52. UCS Cells showed differential cytopathic effects (CPEs) after inoculation of spring viremia of carp virus (SVCV, a negative-strand RNA virus), grass carp reovirus (GCRV, a multi-segmented double-stranded RNA virus) and Rana grylio virus (RGV, a large double-stranded DNA virus), and were indicative of high sensitivities to these three aquatic animal viruses by a virus titration study. The CPE caused by SVCV appeared as rounded and granular Cells, grape-like clusters and small lytic plaques. Characteristic CPE containing plaque-like syncytia was induced by GCRV. RGV-infected Cells produced typical CPE characterized by Cells shrinkage and aggregation, formation of clear plaques and Cell sheet detachment. Furthermore, significant fluorescent signals were observed after UCS Cells were transfected with green fluorescent protein reporter plasmids, and the development of CPE induced by a recombinant RGV, Delta TK-RGV, in UCS Cells was illustrated using a combination of light and fluorescence microscopy. The data from this study suggested that UCS Cell Line can potentially serve as a useful tool for the comparison study of different aquatic animal viruses and the isolation of some newly emerging viruses in Ussuri catfish farming. (C) 2014 Elsevier B.V. All rights reserved.
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characterization and virus susceptibility of a Skin Cell Line from red spotted grouper epinephelus akaara
Fish Physiology and Biochemistry, 2012Co-Authors: Xiaoying Lei, Zhongyuan Chen, Chao Pei, Xiuping Yuan, Qi-ya ZhangAbstract:A red-spotted grouper Epinephelus akaara Skin (RGS) Cell Line was established and characterized. RGS Cells had a normal diploid chromosome number of 2n = 48, the morphology of which was fibroblastic-like in 3 days and epithelial-like over 5 after 16 passages. The Cells multiplied well in Dulbecco's modified Eagle's medium supplemented with 10% of fetal bovine serum at 25A degrees C. Susceptibilities of RGS and grass carp ovary (GCO) Cells to two viruses were tested, and the results showed that the titer of an iridovirus Rana grylio virus (RGV) in RGS Cells was 10(3.5) TCID50 ml(-1), which was much higher than a rhabdovirus spring viremia of carp virus (SVCV) in the Cells (10(0.5) TCID50 ml(-1)). The titers of RGV and SVCV in GCO were 10(6.0) TCID50 ml(-1) and 10(8.0) TCID50 ml(-1), respectively, which were higher than those in RGS Cells. The data may imply that RGS Cells could be selectively resistible to some viruses during infection. RT-PCR analysis of RGV-infected RGS Cells showed that RGV could replicate in RGS Cells. Further study of virus replications in RGS Cells was conducted by electron microscopy and immunofluorescence microscopy has shown that virus particles scattered in the cytoplasm and virus protein appeared in both the cytoplasm and nucleus. The results suggested that RGS Cells could be used as a potential in vitro model to study the cutaneous barrier function against virus infection.
Craig Priest - One of the best experts on this subject based on the ideXlab platform.
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multiparameter toxicity screening on a chip effects of uv radiation and titanium dioxide nanoparticles on hacat Cells
Biomicrofluidics, 2019Co-Authors: Scott Mccormick, Louise E Smith, Amy Holmes, Ziqiu Tong, Enzo Lombi, Nicolas H Voelcker, Craig PriestAbstract:Microfluidic screening is gaining attention as an efficient method for evaluating nanomaterial toxicity. Here, we consider a multiparameter treatment where nanomaterials interact with Cells in the presence of a secondary exposure (UV radiation). The microfluidic device contains channels that permit immobilization of HaCaT Cells (human Skin Cell Line), delivery of titanium dioxide nanoparticles (TNPs), and exposure to a known dose of UV radiation. The effect of single-parameter exposures (UV or TNP) was first studied as a benchmark, and then multiparameter toxicity (UV and TNP) at different concentrations was explored. The results demonstrate a concentration-dependent protective effect of TNP when exposed to UV irradiation.