The Experts below are selected from a list of 17631 Experts worldwide ranked by ideXlab platform
Sheikh A Tasduq - One of the best experts on this subject based on the ideXlab platform.
-
glycyrrhizic acid ga inhibits reactive oxygen species mediated photodamage by blocking er stress and mapk pathway in uv b irradiated human Skin Fibroblasts
Journal of Photochemistry and Photobiology B-biology, 2015Co-Authors: Mufti R Farrukh, Ulashraf Nissar, Peerzada Kaiser, Quadri Afnan, Praduman R Sharma, Shashi Bhushan, Sheikh A TasduqAbstract:Abstract Background Previously we have reported that generation of reactive oxygen species is the prime event responsible for calcium mediated activation of PERK-eIF2α-CHOP pathway and apoptosis in UV-B irradiated human Skin Fibroblasts (Hs68). We have also reported that glycyrrhizic acid (GA) mediates potent photoprotective activity against UV-B – irradiation-induced photodamage in human Skin Fibroblast. Objective In the present study, we aimed to investigate the role of GA in preventing oxidative stress mediated unfolded protein response (UPR) and mitogen activated protein kinases (MAPK) pathway. Methods Human Skin Fibroblast (Hs68) cells were exposed to UV-B radiations in lab conditions. Different parameters of UVB induced cellular and molecular changes were analysed using western-blotting, microscopy and flow cytometry. Results Our results show that GA has strong photoprotective action against UV-B induced cellular damage. It was observed that: (a) Oxidative disturbances and intracellular Ca2+ imbalance induced by UV-B irradiation was significantly restored by GA treatment; (b) activation of PERK-eIF2α-CHOP and MAPK pathway induced by UV-B was significantly blocked by GA; (c) Loss of mitochondrial membrane potential and apoptosis induced by UV-B were reduced by GA treatment. Conclusion Based on the above findings we conclude GA has a highly significant ROS quenching activity, thereby blocking the cascade of events including release of calcium from ER and subsequent ER stress, MAPK pathway and cellular demise. GA offers highly potent anti photodamage effect and can be exploited for cosmetic or therapeutic purposes.
-
glycyrrhizic acid ga inhibits reactive oxygen species mediated photodamage by blocking er stress and mapk pathway in uv b irradiated human Skin Fibroblasts
Journal of Photochemistry and Photobiology B-biology, 2015Co-Authors: Mufti R Farrukh, Ulashraf Nissar, Peerzada Kaiser, Quadri Afnan, Praduman R Sharma, Shashi Bhushan, Sheikh A TasduqAbstract:Abstract Background Previously we have reported that generation of reactive oxygen species is the prime event responsible for calcium mediated activation of PERK-eIF2α-CHOP pathway and apoptosis in UV-B irradiated human Skin Fibroblasts (Hs68). We have also reported that glycyrrhizic acid (GA) mediates potent photoprotective activity against UV-B – irradiation-induced photodamage in human Skin Fibroblast. Objective In the present study, we aimed to investigate the role of GA in preventing oxidative stress mediated unfolded protein response (UPR) and mitogen activated protein kinases (MAPK) pathway. Methods Human Skin Fibroblast (Hs68) cells were exposed to UV-B radiations in lab conditions. Different parameters of UVB induced cellular and molecular changes were analysed using western-blotting, microscopy and flow cytometry. Results Our results show that GA has strong photoprotective action against UV-B induced cellular damage. It was observed that: (a) Oxidative disturbances and intracellular Ca2+ imbalance induced by UV-B irradiation was significantly restored by GA treatment; (b) activation of PERK-eIF2α-CHOP and MAPK pathway induced by UV-B was significantly blocked by GA; (c) Loss of mitochondrial membrane potential and apoptosis induced by UV-B were reduced by GA treatment. Conclusion Based on the above findings we conclude GA has a highly significant ROS quenching activity, thereby blocking the cascade of events including release of calcium from ER and subsequent ER stress, MAPK pathway and cellular demise. GA offers highly potent anti photodamage effect and can be exploited for cosmetic or therapeutic purposes.
-
effect of emblica officinalis fruit against uvb induced photo aging in human Skin Fibroblasts
Journal of Ethnopharmacology, 2010Co-Authors: Mushtaq Dar Adil, Peerzada Kaiser, N K Satti, Afzal Zargar, Ram A Vishwakarma, Sheikh A TasduqAbstract:Abstract Ethnopharmacological relevance Emblica officinalis fruit (EO), commonly known as Amla is a reputed traditional medicine and functional food used in Indian subcontinent. It has long been used in Indian folk medicine to treat liver diseases, stomach ulcers, inflammatory diseases, metabolic disorders, geriatric complaints, Skin disorders and beauty care. Aim of the study Recently, it has been shown to promote pro-collagen content and inhibit matrix metalloproteinase levels in Skin Fibroblast. The aim of the present study was to investigate the efficacy of EO to inhibit UVB-induced photo-aging in human Skin Fibroblasts. Materials and methods Mitochondrial activity of human Skin Fibroblasts was measured by MTT-assay. Quantifications of pro-collagen 1 and matrix metalloproteinase 1 (MMP-1) release were performed by immunoassay techniques. Hyaluronidase inhibition assay was studied in vitro using bovine testicular hyaluronidase and human umbilical cord hyaluronic acid. Cell cycle analysis was performed by flowcytometry using propidium iodide. Results EO stimulated, the otherwise UVB inhibited cellular proliferation and protected pro-collagen 1 against UVB-induced depletion via inhibition of UVB-induced MMP-1 in Skin Fibroblasts (10–40 μg/mL, p > 0.001). EO exhibited inhibitory activity of hyaluronidase (10–40 μg/mL, p > 0.001). Treatment with EO also prevented UVB disturbed cell cycle to normal phase. Conclusion The results of the present study suggests that EO effectively inhibits UVB-induced photo-aging in human Skin Fibroblast via its strong ROS scavenging ability and its therapeutic and cosmetic applications remain to be explored.
Genji Imokawa - One of the best experts on this subject based on the ideXlab platform.
-
Review Biological Mechanisms Underlying the Ultraviolet Radiation-Induced Formation of Skin Wrinkling and Sagging II: Over-Expression of Neprilysin Plays an Essential Role
2016Co-Authors: Genji Imokawa, Hiroaki Nakajima, Koichi IshidaAbstract:Abstract: Our previous studies strongly indicated that the up-regulated activity of Skin Fibroblast-derived elastase plays a pivotal role in wrinkling and/or sagging of the Skin via the impairment of elastic fiber configuration and the subsequent loss of Skin elasticity. Fortunately, we succeeded in identifying human Skin Fibroblast-derived elastase as a previously known enzyme, neprilysin or neutral endopeptidase (NEP). We have also characterized epithelial-mesenchymal paracrine cytokine interactions between UVB-exposed-keratinocytes and dermal Fibroblasts and found that interleukin-1α and granulocyte macrophage colony stimulatory factor (GM-CSF) are intrinsic cytokines secreted by UVB-exposed keratinocytes that stimulate the expression of neprilysin by Fibroblasts. On the other hand, direct UVA exposure of human Fibroblasts significantly stimulates the secretion of IL-6 and also elicits a significant increase in the gene expression of matrix metallo-protease(MMP)-1 as well as neprilysin (to a lesser extent), which is followed by distinct increases in their protein and enzymatic activity levels. Direct UVA exposure of human keratinocytes also stimulates th
-
Review Biological Mechanisms Underlying the Ultraviolet Radiation-Induced Formation of Skin Wrinkling and Sagging I: Reduced Skin Elasticity, Highly Associated with Enhanced
2016Co-Authors: Dermal Elastase Activity, Genji Imokawa, Triggers Wrinkling, Koichi IshidaAbstract:Abstract: The repetitive exposure of Skin to ultraviolet B (UVB) preferentially elicits wrinkling while ultraviolet A (UVA) predominantly elicits sagging. In chronically UVB or UVA-exposed rat Skin there is a similar tortuous deformation of elastic fibers together with decreased Skin elasticity, whose magnitudes are greater in UVB-exposed Skin than in UVA-exposed Skin. Comparison of Skin elasticity with the activity of matrix metalloproteinases (MMPs) in the dermis of ovariectomized rats after UVB or UVA irradiation demonstrates that Skin elasticity is more significantly decreased in ovariectomized rats than in sham-operated rats, which is accompanied by a reciprocal increase in elastase activity but not in the activities of collagenases I or IV. Clinical studies using animal Skin and human facial Skin demonstrated that topical treatment with a specific inhibitor or an inhibitory extract of Skin Fibroblast-derived elastase distinctly attenuates UVB and sunlight-induced formation of wrinkling. Our results strongly indicated that the upregulated activity of Skin Fibroblast-derived elastase plays a pivotal role in wrinkling and/or sagging of the Skin via the impairment of elastic fiber configuration and the subsequent loss of Skin elasticity
-
Biological mechanisms underlying the ultraviolet radiation-induced formation of Skin wrinkling and sagging I: Reduced Skin elasticity, highly associated with enhanced dermal elastase activity, triggers wrinkling and sagging
International Journal of Molecular Sciences, 2015Co-Authors: Genji Imokawa, Koichi IshidaAbstract:The repetitive exposure of Skin to ultraviolet B (UVB) preferentially elicits wrinkling while ultraviolet A (UVA) predominantly elicits sagging. In chronically UVB or UVA-exposed rat Skin there is a similar tortuous deformation of elastic fibers together with decreased Skin elasticity, whose magnitudes are greater in UVB-exposed Skin than in UVA-exposed Skin. Comparison of Skin elasticity with the activity of matrix metalloproteinases (MMPs) in the dermis of ovariectomized rats after UVB or UVA irradiation demonstrates that Skin elasticity is more significantly decreased in ovariectomized rats than in sham-operated rats, which is accompanied by a reciprocal increase in elastase activity but not in the activities of collagenases I or IV. Clinical studies using animal Skin and human facial Skin demonstrated that topical treatment with a specific inhibitor or an inhibitory extract of Skin Fibroblast-derived elastase distinctly attenuates UVB and sunlight-induced formation of wrinkling. Our results strongly indicated that the upregulated activity of Skin Fibroblast-derived elastase plays a pivotal role in wrinkling and/or sagging of the Skin via the impairment of elastic fiber configuration and the subsequent loss of Skin elasticity.
-
Biological mechanisms underlying the ultraviolet radiation-induced formation of Skin wrinkling and sagging II: Over-expression of neprilysin plays an essential role
International Journal of Molecular Sciences, 2015Co-Authors: Genji Imokawa, Hiroaki Nakajima, Koichi IshidaAbstract:Our previous studies strongly indicated that the up-regulated activity of Skin Fibroblast-derived elastase plays a pivotal role in wrinkling and/or sagging of the Skin via the impairment of elastic fiber configuration and the subsequent loss of Skin elasticity. Fortunately, we succeeded in identifying human Skin Fibroblast-derived elastase as a previously known enzyme, neprilysin or neutral endopeptidase (NEP). We have also characterized epithelial-mesenchymal paracrine cytokine interactions between UVB-exposed-keratinocytes and dermal Fibroblasts and found that interleukin-1α and granulocyte macrophage colony stimulatory factor (GM-CSF) are intrinsic cytokines secreted by UVB-exposed keratinocytes that stimulate the expression of neprilysin by Fibroblasts. On the other hand, direct UVA exposure of human Fibroblasts significantly stimulates the secretion of IL-6 and also elicits a significant increase in the gene expression of matrix metallo-protease(MMP)-1 as well as neprilysin (to a lesser extent), which is followed by distinct increases in their protein and enzymatic activity levels. Direct UVA exposure of human keratinocytes also stimulates the secretion of IL-6, IL-8 and GM-CSF but not of IL-1 and endothelin-1. These findings suggest that GM-CSF secreted by UVA-exposed keratinocytes as well as IL-6 secreted by UVA-exposed dermal Fibroblasts play important and additional roles in UVA-induced sagging and wrinkling by up-regulation of neprilysin and MMP-1, respectively, in dermal Fibroblasts.
-
neprilysin is identical to Skin Fibroblast elastase its role in Skin aging and uv responses
Journal of Biological Chemistry, 2010Co-Authors: Naoko Morisaki, Shigeru Moriwaki, Yoriko Sugiyamanakagiri, Keiichi Haketa, Yoshinori Takema, Genji ImokawaAbstract:Abstract Although human Skin Fibroblast (HSF) elastase has been characterized as a membrane-bound metalloproteinase, little is known about its structure, amino acid sequence, and encoding gene. As there are similarities in the molecular weights and inhibitory profiles of HSF elastase and neprilysin (neutral endopeptidase 24.11 (NEP)), in this study we tested the hypothesis that they are identical using immunoprecipitation and transfection methods. An immunoprecipitation study demonstrated that HSF elastase activity co-immunoprecipitated with anti-NEP in lysates of cultured HSF. Transfection of an NEP cDNA expression vector into COS-1 cells elicited the expression of HSF elastase and NEP activities in the transfected cells. These findings strongly suggest that HSF elastase is identical to NEP, which functions mainly in neuron-associated cells to degrade neuropeptides. Analysis of the expression pattern of NEP revealed that its expression was remarkably up-regulated at the gene, protein, and enzymatic activity levels during the replicative senescence of cultured HSF. Further, the activity of NEP was markedly enhanced in a pattern similar to elastase activity during the intrinsic aging of mouse Skin, in UVA-exposed HSF as well as in HSF treated with conditioned medium from UVB-exposed human keratinocytes. Analysis of the cytokine profile for the stimulation of NEP and HSF elastase activities in HSF demonstrated that among the 11 cytokines tested, IL-1α, IL-1β, IL-6, IL-8, and GM-CSF had the potential to significantly stimulate both activities similarly, again supporting the identity of HSF elastase and NEP.
Mengsu Yang - One of the best experts on this subject based on the ideXlab platform.
-
herbal formula astragali radix and rehmanniae radix exerted wound healing effect on human Skin Fibroblast cell line hs27 via the activation of transformation growth factor tgf β pathway and promoting extracellular matrix ecm deposition
Phytomedicine, 2012Co-Authors: Qi Zhang, Chi Chun Fong, Yao Chen, Fan Wei, Chi Man Koon, Kit Man Lau, Pingchung Leung, Clara Biksan Lau, Kwokpui Fung, Mengsu YangAbstract:Astragali Radix (AR) and Rehmanniae Radix (RR) have long been used in traditional Chinese Medicine and as the principal herbs in treating diabetic foot ulcer. In this study, we investigated the effect of NF3, which comprises of AR and RR in the ratio of 2:1(w/w), on Skin Fibroblast cell migration and the activation of selected genes and proteins related to wound healing. Human Skin Fibroblast cell line Hs27 was treated with NF3 at 4 mg/ml for 24h, and in vitro scratch wound healing and quantitative cell migration assays were performed, respectively. The expression of transformation growth factor (TGF-β1) and bone morphogenetic protein 6 (BMP6) in Hs27 cells with or without NF3 treatment was analyzed by western blot analysis. In addition, the expression of a panel of genes involved in human TGF-β signaling pathway was analyzed in Hs27 cells upon NF3 treatment (4 mg/ml, 24 h) by quantitative real-time PCR (qRT-PCR). Furthermore, the expression of several genes and proteins associated with ECM synthesis was investigated by qRT-PCR analysis or/and ELISA techniques. The results suggested that NF3 promoted the migration of human Skin Fibroblast cells. Western blot analysis demonstrated that NF3 up-regulated TGF-β1 and BMP-6 synthesis. qRT-PCR analysis revealed that the expression of 26 genes in Hs27 cells was changed upon NF3 induction, including TGF-β superfamily ligands and down stream effectors genes, and genes involved in TGF/Smad pathway, and Ras/MAPK (non-Smad) pathway. Among the extracellular matrix (ECM)-related molecules, it was found that NF3 up-regulated the expression of type I and III collagens, fibronectin as well as TIMP-1, and down-regulated the MMP-9 expression in Skin Fibroblast cells. This study demonstrated that herb formula NF3 could enhance Skin Fibroblast cell migration and activated genes involved in TGF-β1 pathway. NF3 could regulate gene transcription for extracellular matrix synthesis via the Smad pathway, and gene transcription for cell motility via the Ras/MAPK (non-Smad) pathway.
-
transcriptional profiling of human Skin Fibroblast cell line hs27 induced by herbal formula astragali radix and rehmanniae radix
Journal of Ethnopharmacology, 2011Co-Authors: Qi Zhang, Chi Chun Fong, Yao Chen, Fan Wei, Chi Man Koon, Kit Man Lau, Pingchung Leung, Clara Biksan Lau, Kwokpui Fung, Mengsu YangAbstract:Abstract Ethnopharmacological relevance The herbs Astragali Radix (AR) and Rehmanniae Radix (RR) have long been used in traditional Chinese Medicine and serve as the principal herbs in treating diabetic foot ulcer. Aim of the study Chinese herbal formulus comprising Astragali Radix (AR) and Rehmanniae Radix (RR) have been shown to improve the healing of diabetic foot ulcer through enhancing the viability of primary Fibroblasts in diabetic patients suffering insulin resistance. Our previous study demonstrated that the herbal formula NF3 comprising of AR and RR in the ratio of 2:1 was effective in promoting wound healing in diabetic rats, and in vitro data indicated that the wound healing effects of NF3 might be due to the regulation and coordination of inflammation, angiogenesis and tissue regeneration. However, the underlying molecular mechanism has not been well investigated. In this study, we investigated the cellular and molecular effects of the herbal formula NF3 on human Skin Fibroblast cells. Materials and methods Human Skin Fibroblast cells Hs27 were treated with NF3 ranging from 0 to 8 mg/ml for 24 h, and the cells without NF3 treatment were used as control. Cell proliferation assay and cell cycle analysis were performed. Transcriptional profiles of Hs27 cells upon NF3 treatment were acquired by using a human cDNA microarray containing 10,000 genes, and the signaling pathways differentially regulated by NF3 were identified and analyzed. Results NF3 promoted Hs27 cell proliferation and cell cycle progression. Microarray analysis revealed that 116 genes were differentially expressed upon NF3 treatment. Functional analysis of the genes indicated that NF3 mainly activated Wnt and angiogenesis related pathways, which are directly related to cell proliferation, angiogenesis, extracellular matrix (ECM) formation and inflammation during the process of wound healing. Conclusion This study provides insight into the molecular mechanism of how the herbal formula Astragali Radix and Rehmanniae Radix may serve as potential therapeutics for wound healing.
Koichi Ishida - One of the best experts on this subject based on the ideXlab platform.
-
Review Biological Mechanisms Underlying the Ultraviolet Radiation-Induced Formation of Skin Wrinkling and Sagging II: Over-Expression of Neprilysin Plays an Essential Role
2016Co-Authors: Genji Imokawa, Hiroaki Nakajima, Koichi IshidaAbstract:Abstract: Our previous studies strongly indicated that the up-regulated activity of Skin Fibroblast-derived elastase plays a pivotal role in wrinkling and/or sagging of the Skin via the impairment of elastic fiber configuration and the subsequent loss of Skin elasticity. Fortunately, we succeeded in identifying human Skin Fibroblast-derived elastase as a previously known enzyme, neprilysin or neutral endopeptidase (NEP). We have also characterized epithelial-mesenchymal paracrine cytokine interactions between UVB-exposed-keratinocytes and dermal Fibroblasts and found that interleukin-1α and granulocyte macrophage colony stimulatory factor (GM-CSF) are intrinsic cytokines secreted by UVB-exposed keratinocytes that stimulate the expression of neprilysin by Fibroblasts. On the other hand, direct UVA exposure of human Fibroblasts significantly stimulates the secretion of IL-6 and also elicits a significant increase in the gene expression of matrix metallo-protease(MMP)-1 as well as neprilysin (to a lesser extent), which is followed by distinct increases in their protein and enzymatic activity levels. Direct UVA exposure of human keratinocytes also stimulates th
-
Review Biological Mechanisms Underlying the Ultraviolet Radiation-Induced Formation of Skin Wrinkling and Sagging I: Reduced Skin Elasticity, Highly Associated with Enhanced
2016Co-Authors: Dermal Elastase Activity, Genji Imokawa, Triggers Wrinkling, Koichi IshidaAbstract:Abstract: The repetitive exposure of Skin to ultraviolet B (UVB) preferentially elicits wrinkling while ultraviolet A (UVA) predominantly elicits sagging. In chronically UVB or UVA-exposed rat Skin there is a similar tortuous deformation of elastic fibers together with decreased Skin elasticity, whose magnitudes are greater in UVB-exposed Skin than in UVA-exposed Skin. Comparison of Skin elasticity with the activity of matrix metalloproteinases (MMPs) in the dermis of ovariectomized rats after UVB or UVA irradiation demonstrates that Skin elasticity is more significantly decreased in ovariectomized rats than in sham-operated rats, which is accompanied by a reciprocal increase in elastase activity but not in the activities of collagenases I or IV. Clinical studies using animal Skin and human facial Skin demonstrated that topical treatment with a specific inhibitor or an inhibitory extract of Skin Fibroblast-derived elastase distinctly attenuates UVB and sunlight-induced formation of wrinkling. Our results strongly indicated that the upregulated activity of Skin Fibroblast-derived elastase plays a pivotal role in wrinkling and/or sagging of the Skin via the impairment of elastic fiber configuration and the subsequent loss of Skin elasticity
-
Biological mechanisms underlying the ultraviolet radiation-induced formation of Skin wrinkling and sagging I: Reduced Skin elasticity, highly associated with enhanced dermal elastase activity, triggers wrinkling and sagging
International Journal of Molecular Sciences, 2015Co-Authors: Genji Imokawa, Koichi IshidaAbstract:The repetitive exposure of Skin to ultraviolet B (UVB) preferentially elicits wrinkling while ultraviolet A (UVA) predominantly elicits sagging. In chronically UVB or UVA-exposed rat Skin there is a similar tortuous deformation of elastic fibers together with decreased Skin elasticity, whose magnitudes are greater in UVB-exposed Skin than in UVA-exposed Skin. Comparison of Skin elasticity with the activity of matrix metalloproteinases (MMPs) in the dermis of ovariectomized rats after UVB or UVA irradiation demonstrates that Skin elasticity is more significantly decreased in ovariectomized rats than in sham-operated rats, which is accompanied by a reciprocal increase in elastase activity but not in the activities of collagenases I or IV. Clinical studies using animal Skin and human facial Skin demonstrated that topical treatment with a specific inhibitor or an inhibitory extract of Skin Fibroblast-derived elastase distinctly attenuates UVB and sunlight-induced formation of wrinkling. Our results strongly indicated that the upregulated activity of Skin Fibroblast-derived elastase plays a pivotal role in wrinkling and/or sagging of the Skin via the impairment of elastic fiber configuration and the subsequent loss of Skin elasticity.
-
Biological mechanisms underlying the ultraviolet radiation-induced formation of Skin wrinkling and sagging II: Over-expression of neprilysin plays an essential role
International Journal of Molecular Sciences, 2015Co-Authors: Genji Imokawa, Hiroaki Nakajima, Koichi IshidaAbstract:Our previous studies strongly indicated that the up-regulated activity of Skin Fibroblast-derived elastase plays a pivotal role in wrinkling and/or sagging of the Skin via the impairment of elastic fiber configuration and the subsequent loss of Skin elasticity. Fortunately, we succeeded in identifying human Skin Fibroblast-derived elastase as a previously known enzyme, neprilysin or neutral endopeptidase (NEP). We have also characterized epithelial-mesenchymal paracrine cytokine interactions between UVB-exposed-keratinocytes and dermal Fibroblasts and found that interleukin-1α and granulocyte macrophage colony stimulatory factor (GM-CSF) are intrinsic cytokines secreted by UVB-exposed keratinocytes that stimulate the expression of neprilysin by Fibroblasts. On the other hand, direct UVA exposure of human Fibroblasts significantly stimulates the secretion of IL-6 and also elicits a significant increase in the gene expression of matrix metallo-protease(MMP)-1 as well as neprilysin (to a lesser extent), which is followed by distinct increases in their protein and enzymatic activity levels. Direct UVA exposure of human keratinocytes also stimulates the secretion of IL-6, IL-8 and GM-CSF but not of IL-1 and endothelin-1. These findings suggest that GM-CSF secreted by UVA-exposed keratinocytes as well as IL-6 secreted by UVA-exposed dermal Fibroblasts play important and additional roles in UVA-induced sagging and wrinkling by up-regulation of neprilysin and MMP-1, respectively, in dermal Fibroblasts.
Ulashraf Nissar - One of the best experts on this subject based on the ideXlab platform.
-
glycyrrhizic acid ga inhibits reactive oxygen species mediated photodamage by blocking er stress and mapk pathway in uv b irradiated human Skin Fibroblasts
Journal of Photochemistry and Photobiology B-biology, 2015Co-Authors: Mufti R Farrukh, Ulashraf Nissar, Peerzada Kaiser, Quadri Afnan, Praduman R Sharma, Shashi Bhushan, Sheikh A TasduqAbstract:Abstract Background Previously we have reported that generation of reactive oxygen species is the prime event responsible for calcium mediated activation of PERK-eIF2α-CHOP pathway and apoptosis in UV-B irradiated human Skin Fibroblasts (Hs68). We have also reported that glycyrrhizic acid (GA) mediates potent photoprotective activity against UV-B – irradiation-induced photodamage in human Skin Fibroblast. Objective In the present study, we aimed to investigate the role of GA in preventing oxidative stress mediated unfolded protein response (UPR) and mitogen activated protein kinases (MAPK) pathway. Methods Human Skin Fibroblast (Hs68) cells were exposed to UV-B radiations in lab conditions. Different parameters of UVB induced cellular and molecular changes were analysed using western-blotting, microscopy and flow cytometry. Results Our results show that GA has strong photoprotective action against UV-B induced cellular damage. It was observed that: (a) Oxidative disturbances and intracellular Ca2+ imbalance induced by UV-B irradiation was significantly restored by GA treatment; (b) activation of PERK-eIF2α-CHOP and MAPK pathway induced by UV-B was significantly blocked by GA; (c) Loss of mitochondrial membrane potential and apoptosis induced by UV-B were reduced by GA treatment. Conclusion Based on the above findings we conclude GA has a highly significant ROS quenching activity, thereby blocking the cascade of events including release of calcium from ER and subsequent ER stress, MAPK pathway and cellular demise. GA offers highly potent anti photodamage effect and can be exploited for cosmetic or therapeutic purposes.
-
glycyrrhizic acid ga inhibits reactive oxygen species mediated photodamage by blocking er stress and mapk pathway in uv b irradiated human Skin Fibroblasts
Journal of Photochemistry and Photobiology B-biology, 2015Co-Authors: Mufti R Farrukh, Ulashraf Nissar, Peerzada Kaiser, Quadri Afnan, Praduman R Sharma, Shashi Bhushan, Sheikh A TasduqAbstract:Abstract Background Previously we have reported that generation of reactive oxygen species is the prime event responsible for calcium mediated activation of PERK-eIF2α-CHOP pathway and apoptosis in UV-B irradiated human Skin Fibroblasts (Hs68). We have also reported that glycyrrhizic acid (GA) mediates potent photoprotective activity against UV-B – irradiation-induced photodamage in human Skin Fibroblast. Objective In the present study, we aimed to investigate the role of GA in preventing oxidative stress mediated unfolded protein response (UPR) and mitogen activated protein kinases (MAPK) pathway. Methods Human Skin Fibroblast (Hs68) cells were exposed to UV-B radiations in lab conditions. Different parameters of UVB induced cellular and molecular changes were analysed using western-blotting, microscopy and flow cytometry. Results Our results show that GA has strong photoprotective action against UV-B induced cellular damage. It was observed that: (a) Oxidative disturbances and intracellular Ca2+ imbalance induced by UV-B irradiation was significantly restored by GA treatment; (b) activation of PERK-eIF2α-CHOP and MAPK pathway induced by UV-B was significantly blocked by GA; (c) Loss of mitochondrial membrane potential and apoptosis induced by UV-B were reduced by GA treatment. Conclusion Based on the above findings we conclude GA has a highly significant ROS quenching activity, thereby blocking the cascade of events including release of calcium from ER and subsequent ER stress, MAPK pathway and cellular demise. GA offers highly potent anti photodamage effect and can be exploited for cosmetic or therapeutic purposes.
