The Experts below are selected from a list of 18765 Experts worldwide ranked by ideXlab platform
Lisa A. Delouise - One of the best experts on this subject based on the ideXlab platform.
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Quantification of quantum dot murine Skin Penetration with UVR barrier impairment
Nanotoxicology, 2013Co-Authors: Luke J. Mortensen, Samreen Jatana, Robert Gelein, Anna De Benedetto, Karen L. De Mesy Bentley, Lisa A. Beck, Alison Elder, Lisa A. DelouiseAbstract:AbstractUltraviolet radiation (UVR) Skin exposure is a common exogenous insult that can alter Skin barrier and immune functions. With the growing presence of nanoparticles (NPs) in consumer goods and technological applications the potential for NPs to contact UVR-exposed Skin is increasing. Therefore it is important to understand the effect of UVR on NP Skin Penetration and the potential for systemic translocation. Previous studies qualitatively showed that UVR Skin exposure can increase the Penetration of NPs below the stratum corneum. In this work, an in vivo mouse model was used to quantitatively examine the Skin Penetration of carboxylated (CdSe/ZnS, core/shell) quantum dots (QDs) through intact and UVR barrier-disrupted murine Skin by organ Cd mass analysis. Transepidermal water loss was used to measure the magnitude of the Skin barrier defect as a function of UVR dose and time post-UVR exposure. QDs were applied to mice 3–4 days post-UVR exposure at the peak of the Skin barrier disruption. Our resul...
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Increased in vivo Skin Penetration of quantum dots with UVR and in vitro quantum dot cytotoxicity
Colloidal Quantum Dots for Biomedical Applications IV, 2009Co-Authors: Luke J. Mortensen, Anna De Benedetto, Lisa A. Beck, Hong Zheng, Renea Faulknor, Lisa A. DelouiseAbstract:The growing presence of quantum dots (QD) in a variety of biological, medical, and electronics applications means an increased risk of human exposure in manufacturing, research, and consumer use. However, very few studies have investigated the susceptibility of Skin to Penetration of QD - the most common exposure route- and the results of those that exist are conflicting. This suggests that a technique allowing determination of Skin barrier status and prediction of Skin permeability to QD would be of crucial interest as recent findings have provided evidence of in vitro cytotoxicity and long-term in vivo retention in the body for most QD surface chemistries. Our research focuses on barrier status of the Skin (intact and with ultraviolet radiation induced barrier defect) and its impact on QD Skin Penetration. These model studies are particularly relevant to the common application condition of NP containing sunscreen and SPF cosmetics to UV exposed Skin. Herein we present our initial efforts to develop an in vivo model of nanoparticle Skin Penetration using the SKH-1 hairless mouse with transepidermal water loss (TEWL) to evaluate Skin barrier status and determine its ability to predict QD Penetration. Our results show that ultraviolet radiation increases both TEWL and Skin Penetration of QD. Additionally, we demonstrate cytotoxic potential of QD to Skin cells using a metastatic melanoma cell line. Our research suggests future work in specific targeting of nanoparticles, to prevent or enhance Penetration. This knowledge will be used to develop powerful therapeutic agents, decreased Penetration cosmetic nanoparticles, and precise Skin cancer imaging modalities.
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in vivo Skin Penetration of quantum dot nanoparticles in the murine model the effect of uvr
Nano Letters, 2008Co-Authors: Luke J Mortense, Gunte Oberdorste, Alice P Pentland, Lisa A. DelouiseAbstract:Ultraviolet radiation (UVR) has widespread effects on the biology and integrity of the Skin barrier. Research on the mechanisms that drive these changes, as well as their effect on Skin barrier function, has been ongoing since the 1980s. However, no studies have examined the impact of UVR on nanoparticle Skin Penetration. Nanoparticles (NP) are commonly used in sunscreens and other cosmetics, and since consumer use of sunscreen is often applied to sun damaged Skin, the effect of UVR on NP Skin Penetration is a concern due to potential toxicity. In this study, we investigate NP Skin Penetration by employing an in vivo semiconductor quantum dot nanoparticle (QD) model system. This model system improves NP imaging capabilities and provides additional primary interest due to widespread and expanding use of QD in research applications and manufacturing. In our experiments, carboxylated QD were applied to the Skin of SKH-1 mice in a glycerol vehicle with and without UVR exposure. The Skin collection and penetra...
C Surber - One of the best experts on this subject based on the ideXlab platform.
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Skin Penetration and sun protection factor of five uv filters effect of the vehicle
Skin Pharmacology and Physiology, 2003Co-Authors: E Chatelain, B Gabard, C SurberAbstract:To gain information about efficacy and safety of sunscreens, we compared the Skin Penetration of ultraviolet (UV) filters from two vehicles, i.e. an oil-in-water (O/W) emulsion gel and petrolatum jelly both in vitro and in vivo, as well as the corresponding pharmacological effect, i.e. the sun protection factor (SPF) in vivo. The UV filters studied were benzophenone-3 (BPH), ethylhexyl methoxycinnamate (EHM), butyl methoxydibenzoyl methane, ethylhexyl salicylate and homosalate. The human Skin Penetration of these five chemicals from the two vehicles was determined both in vitro using Franz cells and in vivo using a standardized tape-stripping method. The SPF of the two sunscreens was determined in vivo following the COLIPA guidelines. In vitro none of the filters permeated through the Skin after 6 h of product application and very little could be found in the Skin. BPH and EHM were the only UV filters found in the dermis (both after 30 min and 6 h). An effect of the vehicle could be noticed only for BPH after 30 min in the dermis and 6 h in both dermis and epidermis. In vivo, no differences in the amount of individual UV filters (in % of the applied dose) in the 15 first strips of the stratum corneum (SC) were found following 30 min of application of the formulations; however, the amount of UV filters that were retained in the SC was significantly higher (around 3 times) with the O/W emulsion gel than with the petrolatum jelly. This difference between the two vehicles was also of consequence for the SPF in vivo measured 30 min after application of the products (SPF ≅ 18 with the O/W emulsion gel compared to SPF ≅ 10 with the petrolatum jelly). By choosing the right vehicle or optimizing it, not only sunscreen products can be significantly improved in terms of pharmacological efficacy but the potential toxicological risk associated with the Skin Penetration of UV filters may be significantly reduced.
Luke J Mortense - One of the best experts on this subject based on the ideXlab platform.
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in vivo Skin Penetration of quantum dot nanoparticles in the murine model the effect of uvr
Nano Letters, 2008Co-Authors: Luke J Mortense, Gunte Oberdorste, Alice P Pentland, Lisa A. DelouiseAbstract:Ultraviolet radiation (UVR) has widespread effects on the biology and integrity of the Skin barrier. Research on the mechanisms that drive these changes, as well as their effect on Skin barrier function, has been ongoing since the 1980s. However, no studies have examined the impact of UVR on nanoparticle Skin Penetration. Nanoparticles (NP) are commonly used in sunscreens and other cosmetics, and since consumer use of sunscreen is often applied to sun damaged Skin, the effect of UVR on NP Skin Penetration is a concern due to potential toxicity. In this study, we investigate NP Skin Penetration by employing an in vivo semiconductor quantum dot nanoparticle (QD) model system. This model system improves NP imaging capabilities and provides additional primary interest due to widespread and expanding use of QD in research applications and manufacturing. In our experiments, carboxylated QD were applied to the Skin of SKH-1 mice in a glycerol vehicle with and without UVR exposure. The Skin collection and penetra...
E Chatelain - One of the best experts on this subject based on the ideXlab platform.
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Skin Penetration and sun protection factor of five uv filters effect of the vehicle
Skin Pharmacology and Physiology, 2003Co-Authors: E Chatelain, B Gabard, C SurberAbstract:To gain information about efficacy and safety of sunscreens, we compared the Skin Penetration of ultraviolet (UV) filters from two vehicles, i.e. an oil-in-water (O/W) emulsion gel and petrolatum jelly both in vitro and in vivo, as well as the corresponding pharmacological effect, i.e. the sun protection factor (SPF) in vivo. The UV filters studied were benzophenone-3 (BPH), ethylhexyl methoxycinnamate (EHM), butyl methoxydibenzoyl methane, ethylhexyl salicylate and homosalate. The human Skin Penetration of these five chemicals from the two vehicles was determined both in vitro using Franz cells and in vivo using a standardized tape-stripping method. The SPF of the two sunscreens was determined in vivo following the COLIPA guidelines. In vitro none of the filters permeated through the Skin after 6 h of product application and very little could be found in the Skin. BPH and EHM were the only UV filters found in the dermis (both after 30 min and 6 h). An effect of the vehicle could be noticed only for BPH after 30 min in the dermis and 6 h in both dermis and epidermis. In vivo, no differences in the amount of individual UV filters (in % of the applied dose) in the 15 first strips of the stratum corneum (SC) were found following 30 min of application of the formulations; however, the amount of UV filters that were retained in the SC was significantly higher (around 3 times) with the O/W emulsion gel than with the petrolatum jelly. This difference between the two vehicles was also of consequence for the SPF in vivo measured 30 min after application of the products (SPF ≅ 18 with the O/W emulsion gel compared to SPF ≅ 10 with the petrolatum jelly). By choosing the right vehicle or optimizing it, not only sunscreen products can be significantly improved in terms of pharmacological efficacy but the potential toxicological risk associated with the Skin Penetration of UV filters may be significantly reduced.
Yasunori Morimoto - One of the best experts on this subject based on the ideXlab platform.
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Skin Penetration-enhancing effect of drugs by phonophoresis
Journal of Controlled Release, 1995Co-Authors: Hideo Ueda, Kenji Sugibayashi, Yasunori MorimotoAbstract:The Skin Penetration enhancement of nine drugs by phonophoresis was analyzed using ultrasonic irradiation at 150 kHz in in vitro Skin permeation experiments conducted to elucidate the flux and permeability coefficient of drugs, and hydrodynamic parameters of Skin. The flux of lipophilic drugs after sonication was similar to that before sonication, whereas that for hydrophilic drugs after sonication was increased 6.88-7.43-fold. Permeability coefficients of hydrophilic drugs through full thickness Skin with ultrasound were closer to that through stripped Skin without ultrasound, while that of lipophilic drugs was only slightly changed. A comparison of hydrodynamic parameters of Skin with and without ultrasonication indicated an increase in the aqueous region of the stratum corneum and a constant pore size. Thus, ultrasonication has a great effect on the Skin permeation of hydrophilic drugs which usually have low permeability. ?? 1995.
