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Vesna Kerhin-brkljačić - One of the best experts on this subject based on the ideXlab platform.
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Association of HLA-DRB1 alleles with severity of Sarcoidosis
Genes and Immunity, 2005Co-Authors: Zorana Grubić, Renata Žunec, Tatjana Peroš-golubičić, Vesna Kerhin-brkljačićAbstract:In the present study we investigated distribution of HLA-DRB1 alleles among 90 patients with Sarcoidosis and 141 controls. All individuals were typed by PCR-SSP method. Analysis of DRB1 alleles showed significant differences for DRB1*11 and DRB1*0301 allele. DRB1*11 was significantly more frequent (p=0.0051) in patients with chronic onset of disease in comparison with controls, while higher frequency of DRB1*14 (7.0% vs. 4.3%) and lower frequency of DRB1*07 (3.5% vs. 9.6%) were marginally significant. In patients with acute onset of disease DRB1*0301 allele was significantly more frequent (p=0.0021). Among 36 patients with acute (erythema nodosum-EN) or chronic Skin Sarcoidosis DRB1*0301 allele was also present with statistically significant higher frequency (p=0.00004) compared with controls. However, patients with no-Skin Sarcoidosis (N=54) demonstrated higher frequency of DRB1*11 allele (p=0.026). Patients with chest X-ray stage I showed significantlly higher presence of DRB1*0301 allele (p=0.00064), while patients with chest X-ray stage II and III showed lower presence of DRB1*11 allele (p=0.0155) in comparison to control group. This study demonstrates that DRB1*11 positive patients have higher risk for chronic form of disease and II or III radiologic stage, while DRB1*0301 positive patients have higher risk for acute form of disease, radiologic stage I and EN.
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HLA-DRB1 Alleles In Croatian Sarcoidosis Patients
2005Co-Authors: Tatjana Peroš-golubičić, Zorana Grubić, Vesna Kerhin-brkljačić, Silvana Smojver-ježek, Jasna Tekavec-trkanjecAbstract:Sarcoidosis is a heterogeneous multisystem granulomatous disease that primarily affects the lungs. Numerous studies have reported an association of HLA alleles with Sarcoidosis, with variation of alleles in different ethnic groups. Therefore, to identify the significance of HLA alleles in the development of Sarcoidosis in the Croatian population, we investigated the HLA-DRB1 alleles in patients and healthy control subjects. Ninety patients with Sarcoidosis were diagnosed at the Clinical Hospital for Lung diseases Jordanovac, Zagreb. Diagnosis was based on the presence of typical clinical features, chest X-ray findings and biopsy evidence of granuloma. As a control group we used 141 untreated healthy individuals. All subjects were typed for HLA-DRB1 polymorphism by PCR-SSP method. 1) Analysis of DRB1 alleles between all patients and controls showed significant differences and higher frequencies only for DRB1*0301 allele (p=0.0023), while lower frequencies of DRB1*07 and DRB1*13 alleles were marginally significant. (Fig.1.) 2) Patients were divided into two groups according to the onset of Sarcoidosis: a) acute onset of disease (N=34) ; b) chronic onset of disease (N=56). Allele DRB1*0301 was significantly more frequent among patients with acute onset of disease in comparison with controls (18.6 % vs. 6.4% ; p=0.0021), while DRB1*11 was significantly more frequent in patients with chronic onset of disease in comparison with controls (28.1% vs. 15.6% ; p=0.0051). Differences in distribution of DRB1*11 alleles among two patient's groups did not reach significant p value (Fig. 2.) 3) The distribution of HLA-DRB1 alleles into two groups of patients classified on the basis of the chest X-ray stage is presented in the Figure 3. Patients with chest X-ray stage I (N=34) showed significant higher presence of DRB1*0301 alleles in comparison to controls (20.6% vs. 6.4% ; p=0.00064), but not in comparison with patients with chest X-ray stage II or III ( 20.6% vs. 12.5%, p>0, 05). On the other hand, patients with chest X- ray stage II or III showed significantly higher presence of DRB1*11 allele when compared to controls ( 26.8% vs. 15.6% ; p=0.015), but not in comparison with the patients with chest X-ray stage I (26.8% vs. 17.7%, p>0.05). 4) Among 36 patients with acute or chronic Skin Sarcoidosis DRB1*0301 allele was also present with statistically significant higher frequency compared with controls (23.6% vs. 6.4% ; p=0.00004)(Fig. 4). Patients with no Skin Sarcoidosis showed significantly higher frequency of DRB1*11 allele ( 26.9% vs. 15.6% ; p=0.0164) and lower frequency of DRB1*13 allele (4.6 vs. 12.8% ; p=0.0253). Out of 19 patients (21.1%) with EN, 9 patients (47.4%) were DRB1*0301 positive. DISCUSSION Our data on the association of DRB1*0301 allele and acute form of disease are in good agreement with Scandinavian and German studies in which association with HLA-DR3 was also observed. Those studies as well as the studies from UK, Poland and Czech Republic have also reported that DRB1*14 and DRB1*15 as more frequent among these patients, but that is not the case among ours pts. Increased frequency of DRB1*11 allele observed among Croatian patients with chronic onset of Sarcoidosis and chest X-ray stage II or III is in concordance with data from USA and India. This study demonstrates that DRB1*0301 positive patients have higher risk for acute onset of the disease, radiological stage I and Skin Sarcoidosis, especially EN, and that DRB1*11 positive patients have higher risk for developing chronic onset of the disease and radiological stage II and III. Interpolating these findings with known data on the predictors of outcome, we suggest that HLA-DRB1 associated alleles could be used as possible predictors of outcome in Sarcoidosis patients.
Zorana Grubić - One of the best experts on this subject based on the ideXlab platform.
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Association of HLA-DRB1 alleles with severity of Sarcoidosis
Genes and Immunity, 2005Co-Authors: Zorana Grubić, Renata Žunec, Tatjana Peroš-golubičić, Vesna Kerhin-brkljačićAbstract:In the present study we investigated distribution of HLA-DRB1 alleles among 90 patients with Sarcoidosis and 141 controls. All individuals were typed by PCR-SSP method. Analysis of DRB1 alleles showed significant differences for DRB1*11 and DRB1*0301 allele. DRB1*11 was significantly more frequent (p=0.0051) in patients with chronic onset of disease in comparison with controls, while higher frequency of DRB1*14 (7.0% vs. 4.3%) and lower frequency of DRB1*07 (3.5% vs. 9.6%) were marginally significant. In patients with acute onset of disease DRB1*0301 allele was significantly more frequent (p=0.0021). Among 36 patients with acute (erythema nodosum-EN) or chronic Skin Sarcoidosis DRB1*0301 allele was also present with statistically significant higher frequency (p=0.00004) compared with controls. However, patients with no-Skin Sarcoidosis (N=54) demonstrated higher frequency of DRB1*11 allele (p=0.026). Patients with chest X-ray stage I showed significantlly higher presence of DRB1*0301 allele (p=0.00064), while patients with chest X-ray stage II and III showed lower presence of DRB1*11 allele (p=0.0155) in comparison to control group. This study demonstrates that DRB1*11 positive patients have higher risk for chronic form of disease and II or III radiologic stage, while DRB1*0301 positive patients have higher risk for acute form of disease, radiologic stage I and EN.
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HLA-DRB1 Alleles In Croatian Sarcoidosis Patients
2005Co-Authors: Tatjana Peroš-golubičić, Zorana Grubić, Vesna Kerhin-brkljačić, Silvana Smojver-ježek, Jasna Tekavec-trkanjecAbstract:Sarcoidosis is a heterogeneous multisystem granulomatous disease that primarily affects the lungs. Numerous studies have reported an association of HLA alleles with Sarcoidosis, with variation of alleles in different ethnic groups. Therefore, to identify the significance of HLA alleles in the development of Sarcoidosis in the Croatian population, we investigated the HLA-DRB1 alleles in patients and healthy control subjects. Ninety patients with Sarcoidosis were diagnosed at the Clinical Hospital for Lung diseases Jordanovac, Zagreb. Diagnosis was based on the presence of typical clinical features, chest X-ray findings and biopsy evidence of granuloma. As a control group we used 141 untreated healthy individuals. All subjects were typed for HLA-DRB1 polymorphism by PCR-SSP method. 1) Analysis of DRB1 alleles between all patients and controls showed significant differences and higher frequencies only for DRB1*0301 allele (p=0.0023), while lower frequencies of DRB1*07 and DRB1*13 alleles were marginally significant. (Fig.1.) 2) Patients were divided into two groups according to the onset of Sarcoidosis: a) acute onset of disease (N=34) ; b) chronic onset of disease (N=56). Allele DRB1*0301 was significantly more frequent among patients with acute onset of disease in comparison with controls (18.6 % vs. 6.4% ; p=0.0021), while DRB1*11 was significantly more frequent in patients with chronic onset of disease in comparison with controls (28.1% vs. 15.6% ; p=0.0051). Differences in distribution of DRB1*11 alleles among two patient's groups did not reach significant p value (Fig. 2.) 3) The distribution of HLA-DRB1 alleles into two groups of patients classified on the basis of the chest X-ray stage is presented in the Figure 3. Patients with chest X-ray stage I (N=34) showed significant higher presence of DRB1*0301 alleles in comparison to controls (20.6% vs. 6.4% ; p=0.00064), but not in comparison with patients with chest X-ray stage II or III ( 20.6% vs. 12.5%, p>0, 05). On the other hand, patients with chest X- ray stage II or III showed significantly higher presence of DRB1*11 allele when compared to controls ( 26.8% vs. 15.6% ; p=0.015), but not in comparison with the patients with chest X-ray stage I (26.8% vs. 17.7%, p>0.05). 4) Among 36 patients with acute or chronic Skin Sarcoidosis DRB1*0301 allele was also present with statistically significant higher frequency compared with controls (23.6% vs. 6.4% ; p=0.00004)(Fig. 4). Patients with no Skin Sarcoidosis showed significantly higher frequency of DRB1*11 allele ( 26.9% vs. 15.6% ; p=0.0164) and lower frequency of DRB1*13 allele (4.6 vs. 12.8% ; p=0.0253). Out of 19 patients (21.1%) with EN, 9 patients (47.4%) were DRB1*0301 positive. DISCUSSION Our data on the association of DRB1*0301 allele and acute form of disease are in good agreement with Scandinavian and German studies in which association with HLA-DR3 was also observed. Those studies as well as the studies from UK, Poland and Czech Republic have also reported that DRB1*14 and DRB1*15 as more frequent among these patients, but that is not the case among ours pts. Increased frequency of DRB1*11 allele observed among Croatian patients with chronic onset of Sarcoidosis and chest X-ray stage II or III is in concordance with data from USA and India. This study demonstrates that DRB1*0301 positive patients have higher risk for acute onset of the disease, radiological stage I and Skin Sarcoidosis, especially EN, and that DRB1*11 positive patients have higher risk for developing chronic onset of the disease and radiological stage II and III. Interpolating these findings with known data on the predictors of outcome, we suggest that HLA-DRB1 associated alleles could be used as possible predictors of outcome in Sarcoidosis patients.
Richard P. Spencer - One of the best experts on this subject based on the ideXlab platform.
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Lupus Pernio. Radiogallium imaging in a patient with chronic cutaneous Sarcoidosis
Clinical nuclear medicine, 1994Co-Authors: Stephen B. Sulavik, Diane L Whitaker, Richard P. SpencerAbstract:A 37-year-old man with a history of chronic Skin Sarcoidosis had the classic triad of lupus pernio. Each of the lesions (nose, ears, and hands) revealed avid radiogallium accumulation. In addition, both the panda sign and the lambda sign of hilar-mediastinal lymph node uptake of radiogallium were present, despite normal results of chest radiography. Several other lymph node chains were also involved. The case can serve as a baseline to determine if other instances of lupus pernio have such a wide systemic distribution of radiogallium uptake.
Jean-françois Bernaudin - One of the best experts on this subject based on the ideXlab platform.
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Detection of silica and calcium carbonate deposits in granulomatous areas of Skin Sarcoidosis by μFourier transform infrared spectroscopy and Field Emission Scanning Electron Microscopy coupled with Energy Dispersive X-ray Spectroscopy analysis
Comptes Rendus Chimie, 2016Co-Authors: Hester Colboc, Dominique Bazin, Philippe Moguelet, Vincent Frochot, Raphael Weil, Letavernier Emmanuel, Chantal Jouanneau, Camille Francès, Claude Bachmeyer, Jean-françois BernaudinAbstract:Sarcoidosis is a multisystem inflammatory disease affecting different organs particularly lung, Skin, eyes and joints. Characterized by noncaseating epithelioid granulomas, Sarcoidosis is considered to be caused by a complex interplay between genetics and environmental agents while it still remains a disease of unknown etiology. 10 Skin biopsies from patients with cutaneous Sarcoidosis were included in the study. After polarized light examination (PLE) through optical microscopy, these Skin biopsies have been investigated through mFourier transform (FTIR) infrared spectroscopy and Field Emission Scanning Electron Microscopy coupled with Energy Dispersive X-ray Spectroscopy (FE-SEM/EDX). Three biopsies showed a refractive material at PLE. FTIR and FE-SEM/EDX analyses indicate the presence of silica at the center of the granulomas in these three biopsies. Another striking result is related to the presence of calcite, a calcium carbonate at the periphery of the granulomas. To our best knowledge, this is the first time that the presence of this calcium carbonate has been reported. Such description at the submicrometer scale paves the way for a better understanding of the physicochemical processes related to Sarcoidosis and will help clinicians to develop new diagnostic tools.
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Detection of silica and calcium carbonate deposits in granulomatous areas of Skin Sarcoidosis by μFourier transform infrared spectroscopy and Field Emission Scanning Electron Microscopy coupled with Energy Dispersive X-ray Spectroscopy analysis
Comptes Rendus Chimie, 2016Co-Authors: Hester Colboc, Dominique Bazin, Philippe Moguelet, Vincent Frochot, Raphael Weil, Chantal Jouanneau, Camille Francès, Claude Bachmeyer, Emmanuel Letavernier, Jean-françois BernaudinAbstract:International audienceSarcoidosis is a multisystem inflammatory disease affecting different organs particularly lung, Skin, eyes and joints. Characterized by noncaseating epithelioid granulomas, Sarcoidosis is considered to be caused by a complex interplay between genetics and environmental agents while it still remains a disease of unknown etiology. 10 Skin biopsies from patients with cutaneous Sarcoidosis were included in the study. After polarized light examination (PLE) through optical microscopy, these Skin biopsies have been investigated through mFourier transform (FTIR) infrared spectroscopy and Field Emission Scanning Electron Microscopy coupled with Energy Dispersive X-ray Spectroscopy (FE-SEM/EDX). Three biopsies showed a refractive material at PLE. FTIR and FE-SEM/EDX analyses indicate the presence of silica at the center of the granulomas in these three biopsies. Another striking result is related to the presence of calcite, a calcium carbonate at the periphery of the granulomas. To our best knowledge, this is the first time that the presence of this calcium carbonate has been reported. Such description at the submicrometer scale paves the way for a better understanding of the physicochemical processes related to Sarcoidosis and will help clinicians to develop new diagnostic tools
Tatjana Peroš-golubičić - One of the best experts on this subject based on the ideXlab platform.
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Association of HLA-DRB1 alleles with severity of Sarcoidosis
Genes and Immunity, 2005Co-Authors: Zorana Grubić, Renata Žunec, Tatjana Peroš-golubičić, Vesna Kerhin-brkljačićAbstract:In the present study we investigated distribution of HLA-DRB1 alleles among 90 patients with Sarcoidosis and 141 controls. All individuals were typed by PCR-SSP method. Analysis of DRB1 alleles showed significant differences for DRB1*11 and DRB1*0301 allele. DRB1*11 was significantly more frequent (p=0.0051) in patients with chronic onset of disease in comparison with controls, while higher frequency of DRB1*14 (7.0% vs. 4.3%) and lower frequency of DRB1*07 (3.5% vs. 9.6%) were marginally significant. In patients with acute onset of disease DRB1*0301 allele was significantly more frequent (p=0.0021). Among 36 patients with acute (erythema nodosum-EN) or chronic Skin Sarcoidosis DRB1*0301 allele was also present with statistically significant higher frequency (p=0.00004) compared with controls. However, patients with no-Skin Sarcoidosis (N=54) demonstrated higher frequency of DRB1*11 allele (p=0.026). Patients with chest X-ray stage I showed significantlly higher presence of DRB1*0301 allele (p=0.00064), while patients with chest X-ray stage II and III showed lower presence of DRB1*11 allele (p=0.0155) in comparison to control group. This study demonstrates that DRB1*11 positive patients have higher risk for chronic form of disease and II or III radiologic stage, while DRB1*0301 positive patients have higher risk for acute form of disease, radiologic stage I and EN.
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HLA-DRB1 Alleles In Croatian Sarcoidosis Patients
2005Co-Authors: Tatjana Peroš-golubičić, Zorana Grubić, Vesna Kerhin-brkljačić, Silvana Smojver-ježek, Jasna Tekavec-trkanjecAbstract:Sarcoidosis is a heterogeneous multisystem granulomatous disease that primarily affects the lungs. Numerous studies have reported an association of HLA alleles with Sarcoidosis, with variation of alleles in different ethnic groups. Therefore, to identify the significance of HLA alleles in the development of Sarcoidosis in the Croatian population, we investigated the HLA-DRB1 alleles in patients and healthy control subjects. Ninety patients with Sarcoidosis were diagnosed at the Clinical Hospital for Lung diseases Jordanovac, Zagreb. Diagnosis was based on the presence of typical clinical features, chest X-ray findings and biopsy evidence of granuloma. As a control group we used 141 untreated healthy individuals. All subjects were typed for HLA-DRB1 polymorphism by PCR-SSP method. 1) Analysis of DRB1 alleles between all patients and controls showed significant differences and higher frequencies only for DRB1*0301 allele (p=0.0023), while lower frequencies of DRB1*07 and DRB1*13 alleles were marginally significant. (Fig.1.) 2) Patients were divided into two groups according to the onset of Sarcoidosis: a) acute onset of disease (N=34) ; b) chronic onset of disease (N=56). Allele DRB1*0301 was significantly more frequent among patients with acute onset of disease in comparison with controls (18.6 % vs. 6.4% ; p=0.0021), while DRB1*11 was significantly more frequent in patients with chronic onset of disease in comparison with controls (28.1% vs. 15.6% ; p=0.0051). Differences in distribution of DRB1*11 alleles among two patient's groups did not reach significant p value (Fig. 2.) 3) The distribution of HLA-DRB1 alleles into two groups of patients classified on the basis of the chest X-ray stage is presented in the Figure 3. Patients with chest X-ray stage I (N=34) showed significant higher presence of DRB1*0301 alleles in comparison to controls (20.6% vs. 6.4% ; p=0.00064), but not in comparison with patients with chest X-ray stage II or III ( 20.6% vs. 12.5%, p>0, 05). On the other hand, patients with chest X- ray stage II or III showed significantly higher presence of DRB1*11 allele when compared to controls ( 26.8% vs. 15.6% ; p=0.015), but not in comparison with the patients with chest X-ray stage I (26.8% vs. 17.7%, p>0.05). 4) Among 36 patients with acute or chronic Skin Sarcoidosis DRB1*0301 allele was also present with statistically significant higher frequency compared with controls (23.6% vs. 6.4% ; p=0.00004)(Fig. 4). Patients with no Skin Sarcoidosis showed significantly higher frequency of DRB1*11 allele ( 26.9% vs. 15.6% ; p=0.0164) and lower frequency of DRB1*13 allele (4.6 vs. 12.8% ; p=0.0253). Out of 19 patients (21.1%) with EN, 9 patients (47.4%) were DRB1*0301 positive. DISCUSSION Our data on the association of DRB1*0301 allele and acute form of disease are in good agreement with Scandinavian and German studies in which association with HLA-DR3 was also observed. Those studies as well as the studies from UK, Poland and Czech Republic have also reported that DRB1*14 and DRB1*15 as more frequent among these patients, but that is not the case among ours pts. Increased frequency of DRB1*11 allele observed among Croatian patients with chronic onset of Sarcoidosis and chest X-ray stage II or III is in concordance with data from USA and India. This study demonstrates that DRB1*0301 positive patients have higher risk for acute onset of the disease, radiological stage I and Skin Sarcoidosis, especially EN, and that DRB1*11 positive patients have higher risk for developing chronic onset of the disease and radiological stage II and III. Interpolating these findings with known data on the predictors of outcome, we suggest that HLA-DRB1 associated alleles could be used as possible predictors of outcome in Sarcoidosis patients.