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B Klosterhalfen - One of the best experts on this subject based on the ideXlab platform.

  • impaired balance of type i and type iii procollagen mrna in cultured fibroblasts of patients with incisional hernia
    Surgery, 2002
    Co-Authors: R S Bhardwaj, B Klosterhalfen, R Rosch, Peter Rene Merten, U Klinge
    Abstract:

    Background. Recent findings of an impaired protein ratio of type I to type III procollagen showed a disturbed collagen metabolism in incisional hernia development. We analyzed the type I and type III procollagen messenger RNA to investigate whether these findings represent the altered extracellular matrix or a primary defect at the transcriptional level. Methods. We examined cultured Skin fibroblasts of patients with incisional or recurrent incisional hernia in comparison with those without any previous incision (control) and those with a Skin Scar without clinical appearance of a hernia (Scar). Immunohistochemical detection of a lowered protein ratio of type I and type III collagen in the hernia Skin tissue leads to mRNA expression analysis. The procollagen mRNA and the ratio of type I to type III procollagen mRNA are detected by reverse transcriptase-polymerase chain reaction and Northern blot analysis, the collagens type I and III by Western blot analysis. Results. Reverse transcriptase-polymerase chain reaction revealed an increase of type I procollagen mRNA in the incisional and recurrent hernia (0.90 ± 0.04 and 1.19 ± 0.04, respectively) compared with stable Scar (0.54 ± 0.02) or healthy tissue (0.43 ± 0.01). The obvious rise of type III procollagen mRNA to 4.13 ± 0.04 for incisional, 6.02 ± 0.03 for recurrent hernia, 2.29 ± 0.04 for stable Scar, and 1.72 ± 0.03 for the healthy tissue showed a significantly decreased ratio of type I to type III procollagen mRNA in the hernia patients as compared with the controls (P <.01). By Western blot analysis, an increase of type I and type III collagen protein and a significant rise in the corresponding ratio in the recurrent hernia group were detected. Conclusions. The altered synthesis of type I and type III collagen in cultured Skin fibroblasts suggests a disorder of collagen metabolism, at least in patients with recurrent hernia. Hence, a basically impaired wound healing process is likely to contribute to the unsatisfactory results of incisional hernia repair. (Surgery 2002;131:324-31.)

  • recurrent inguinal hernia disease of the collagen matrix
    World Journal of Surgery, 2002
    Co-Authors: Hong Zheng, U Klinge, V Schumpelick, R S Bhardwaj, Reiner Kasperk, B Klosterhalfen
    Abstract:

    The aim of this study was to investigate the collagen matrix in recurrent inguinal hernias. Total ribonucleic acid (RNA) was extracted from Skin fibroblasts of three groups (control group I = healthy Skin; control group II = plain Skin Scar; recurrent inguinal hernia group = Skin of recurrent inguinal hernias; each n = 5). Reverse transcription-polymerase chain reaction (RT-PCR) and Northern blot analysis were used to investigate the expression of procollagen type I/- III, MMP-1, and MMP-13 mRNAs. Both ratios of procollagen types I to III mRNAs and collagen types I to III were apparently decreased in the recurrent hernia group compared to those of both control groups (p <0.01). Significant differences were caused by the increase of both procollagen type III mRNA and collagen type III protein synthesis. A concomitant increase of MMP-1 and MMP-13 mRNAs and proteins was also observed in the recurrent hernia group and showed significant differences compared to those of both control groups I and II, respectively (p <0.01). In conclusion, the decreased ratio of collagen types I to III seems not only to be the result of a relative increase in the levels of type III procollagen mRNA but also may be the result of an increase of MMP-1 and MMP-13. The data of the present study strongly suggest recurrent inguinal hernias to be a disease of the collagen matrix and result in a clearer understanding of the underlying pathophysiology and may support specific therapeutic strategies in hernia surgery (e.g., surgical meshes).

  • collagen i iii and matrix metalloproteinases mmp 1 and 13 in the fascia of patients with incisional hernias
    Journal of Investigative Surgery, 2001
    Co-Authors: U Klinge, Z Y Si, H Zheng, V Schumpelick, R S Bhardwaj, B Klosterhalfen
    Abstract:

    The late appearance of incisional hernias several years after laparotomy and the high recurrence rates after operation strongly imply the presence of a disorder of the connective tissue, although a specific defect in patients with incisional hernias has not yet been identified. In the present study we used both immunohistochemistry and Western blot analysis to evaluate the ratio of collagen I and III and the expression of the metalloproteinases (MMP) 1 and 13 in the fascia of patients with incisional or recurrent incisional hernias. Samples of healthy Skin or stable Skin Scar in patients without hernias served as controls. Altogether, our data indicated a significantly decreased ratio of collagen I/III in the fascia of patients with incisional hernias and recurrent incisional hernias. Furthermore, in these patients the expression of MMP-1 was decreased compared to the controls, whereas MMP-13 could not be detected in any fascia sample, with or without hernias present. For the first time, our results give ...

U Klinge - One of the best experts on this subject based on the ideXlab platform.

  • impaired balance of type i and type iii procollagen mrna in cultured fibroblasts of patients with incisional hernia
    Surgery, 2002
    Co-Authors: R S Bhardwaj, B Klosterhalfen, R Rosch, Peter Rene Merten, U Klinge
    Abstract:

    Background. Recent findings of an impaired protein ratio of type I to type III procollagen showed a disturbed collagen metabolism in incisional hernia development. We analyzed the type I and type III procollagen messenger RNA to investigate whether these findings represent the altered extracellular matrix or a primary defect at the transcriptional level. Methods. We examined cultured Skin fibroblasts of patients with incisional or recurrent incisional hernia in comparison with those without any previous incision (control) and those with a Skin Scar without clinical appearance of a hernia (Scar). Immunohistochemical detection of a lowered protein ratio of type I and type III collagen in the hernia Skin tissue leads to mRNA expression analysis. The procollagen mRNA and the ratio of type I to type III procollagen mRNA are detected by reverse transcriptase-polymerase chain reaction and Northern blot analysis, the collagens type I and III by Western blot analysis. Results. Reverse transcriptase-polymerase chain reaction revealed an increase of type I procollagen mRNA in the incisional and recurrent hernia (0.90 ± 0.04 and 1.19 ± 0.04, respectively) compared with stable Scar (0.54 ± 0.02) or healthy tissue (0.43 ± 0.01). The obvious rise of type III procollagen mRNA to 4.13 ± 0.04 for incisional, 6.02 ± 0.03 for recurrent hernia, 2.29 ± 0.04 for stable Scar, and 1.72 ± 0.03 for the healthy tissue showed a significantly decreased ratio of type I to type III procollagen mRNA in the hernia patients as compared with the controls (P <.01). By Western blot analysis, an increase of type I and type III collagen protein and a significant rise in the corresponding ratio in the recurrent hernia group were detected. Conclusions. The altered synthesis of type I and type III collagen in cultured Skin fibroblasts suggests a disorder of collagen metabolism, at least in patients with recurrent hernia. Hence, a basically impaired wound healing process is likely to contribute to the unsatisfactory results of incisional hernia repair. (Surgery 2002;131:324-31.)

  • recurrent inguinal hernia disease of the collagen matrix
    World Journal of Surgery, 2002
    Co-Authors: Hong Zheng, U Klinge, V Schumpelick, R S Bhardwaj, Reiner Kasperk, B Klosterhalfen
    Abstract:

    The aim of this study was to investigate the collagen matrix in recurrent inguinal hernias. Total ribonucleic acid (RNA) was extracted from Skin fibroblasts of three groups (control group I = healthy Skin; control group II = plain Skin Scar; recurrent inguinal hernia group = Skin of recurrent inguinal hernias; each n = 5). Reverse transcription-polymerase chain reaction (RT-PCR) and Northern blot analysis were used to investigate the expression of procollagen type I/- III, MMP-1, and MMP-13 mRNAs. Both ratios of procollagen types I to III mRNAs and collagen types I to III were apparently decreased in the recurrent hernia group compared to those of both control groups (p <0.01). Significant differences were caused by the increase of both procollagen type III mRNA and collagen type III protein synthesis. A concomitant increase of MMP-1 and MMP-13 mRNAs and proteins was also observed in the recurrent hernia group and showed significant differences compared to those of both control groups I and II, respectively (p <0.01). In conclusion, the decreased ratio of collagen types I to III seems not only to be the result of a relative increase in the levels of type III procollagen mRNA but also may be the result of an increase of MMP-1 and MMP-13. The data of the present study strongly suggest recurrent inguinal hernias to be a disease of the collagen matrix and result in a clearer understanding of the underlying pathophysiology and may support specific therapeutic strategies in hernia surgery (e.g., surgical meshes).

  • collagen i iii and matrix metalloproteinases mmp 1 and 13 in the fascia of patients with incisional hernias
    Journal of Investigative Surgery, 2001
    Co-Authors: U Klinge, Z Y Si, H Zheng, V Schumpelick, R S Bhardwaj, B Klosterhalfen
    Abstract:

    The late appearance of incisional hernias several years after laparotomy and the high recurrence rates after operation strongly imply the presence of a disorder of the connective tissue, although a specific defect in patients with incisional hernias has not yet been identified. In the present study we used both immunohistochemistry and Western blot analysis to evaluate the ratio of collagen I and III and the expression of the metalloproteinases (MMP) 1 and 13 in the fascia of patients with incisional or recurrent incisional hernias. Samples of healthy Skin or stable Skin Scar in patients without hernias served as controls. Altogether, our data indicated a significantly decreased ratio of collagen I/III in the fascia of patients with incisional hernias and recurrent incisional hernias. Furthermore, in these patients the expression of MMP-1 was decreased compared to the controls, whereas MMP-13 could not be detected in any fascia sample, with or without hernias present. For the first time, our results give ...

Cuiping Zhang - One of the best experts on this subject based on the ideXlab platform.

  • will stem cells bring hope to pathological Skin Scar treatment
    Cytotherapy, 2016
    Co-Authors: Cuiping Zhang
    Abstract:

    Pathological Skin Scars, such as keloids, aesthetically and psychosocially affect patients. The quest for Scar reduction and the increasing recognition of patient satisfaction has led to the continued exploration of Scar treatment. Stem cells are a promising source for tissue repair and regeneration. The multi-potency and secretory functions of these cells could offer possible treatments for pathological Scars and have been examined in recent studies. Here, we analyze the factors that influence the formation of pathological Skin Scars, summarize recent research on pathological Scar treatment with stem cells and elaborate on the possible mechanisms of this treatment. Additionally, other effects of stem cell treatments are also presented while evaluating potential side effects of stem cell-based pathological Scar treatments. Thus, this review may provide meaningful guidance in the clinic for Scar treatments with stem cells.

Ying Cheong - One of the best experts on this subject based on the ideXlab platform.

  • are Skin Scar characteristics associated with the degree of pelvic adhesions at laparoscopy
    Fertility and Sterility, 2014
    Co-Authors: Linden Stocker, Jane E Glazebrook, Ying Cheong
    Abstract:

    Objective To investigate whether individual or a combination of abdominal surgical Scar characteristics can predict the severity and extent of intra-abdominal adhesions. Design A prospective cohort study. Setting A tertiary referral center in the United Kingdom. Patient(s) One hundred women who had previously undergone abdominopelvic surgery and were undergoing an elective laparoscopic gynecologic operations. Intervention(s) Abdominal Scars were evaluated preoperatively using the modified Manchester Scar Questionnaire Adhesions were assessed intraoperatively and compared with the cutaneous findings. Main Outcome Measure(s) Presence and severity of intra-abdominal adhesions. Result(s) Of 100 women recruited into this study, 71 (71%) women were found to have intra-abdominal Aadhesions, and 29 (29%) had no adhesions. Women who had more than one abdominal Scar, a palpable Scar, and/or a longer Scar were most likely to have pelvic adhesions during the current surgery. Women with the highest mean Scar scores also had a greater total adhesion score. Conclusion(s) Adhesions are a common postoperative consequence of open or laparoscopic surgery. Skin Scar characteristics are associated with the presence and degree of pelvic adhesions. Future studies should examine whether these characteristics can be used as a preoperative predictive tool to facilitate surgical decision-making and elective operating room organization.

R S Bhardwaj - One of the best experts on this subject based on the ideXlab platform.

  • impaired balance of type i and type iii procollagen mrna in cultured fibroblasts of patients with incisional hernia
    Surgery, 2002
    Co-Authors: R S Bhardwaj, B Klosterhalfen, R Rosch, Peter Rene Merten, U Klinge
    Abstract:

    Background. Recent findings of an impaired protein ratio of type I to type III procollagen showed a disturbed collagen metabolism in incisional hernia development. We analyzed the type I and type III procollagen messenger RNA to investigate whether these findings represent the altered extracellular matrix or a primary defect at the transcriptional level. Methods. We examined cultured Skin fibroblasts of patients with incisional or recurrent incisional hernia in comparison with those without any previous incision (control) and those with a Skin Scar without clinical appearance of a hernia (Scar). Immunohistochemical detection of a lowered protein ratio of type I and type III collagen in the hernia Skin tissue leads to mRNA expression analysis. The procollagen mRNA and the ratio of type I to type III procollagen mRNA are detected by reverse transcriptase-polymerase chain reaction and Northern blot analysis, the collagens type I and III by Western blot analysis. Results. Reverse transcriptase-polymerase chain reaction revealed an increase of type I procollagen mRNA in the incisional and recurrent hernia (0.90 ± 0.04 and 1.19 ± 0.04, respectively) compared with stable Scar (0.54 ± 0.02) or healthy tissue (0.43 ± 0.01). The obvious rise of type III procollagen mRNA to 4.13 ± 0.04 for incisional, 6.02 ± 0.03 for recurrent hernia, 2.29 ± 0.04 for stable Scar, and 1.72 ± 0.03 for the healthy tissue showed a significantly decreased ratio of type I to type III procollagen mRNA in the hernia patients as compared with the controls (P <.01). By Western blot analysis, an increase of type I and type III collagen protein and a significant rise in the corresponding ratio in the recurrent hernia group were detected. Conclusions. The altered synthesis of type I and type III collagen in cultured Skin fibroblasts suggests a disorder of collagen metabolism, at least in patients with recurrent hernia. Hence, a basically impaired wound healing process is likely to contribute to the unsatisfactory results of incisional hernia repair. (Surgery 2002;131:324-31.)

  • recurrent inguinal hernia disease of the collagen matrix
    World Journal of Surgery, 2002
    Co-Authors: Hong Zheng, U Klinge, V Schumpelick, R S Bhardwaj, Reiner Kasperk, B Klosterhalfen
    Abstract:

    The aim of this study was to investigate the collagen matrix in recurrent inguinal hernias. Total ribonucleic acid (RNA) was extracted from Skin fibroblasts of three groups (control group I = healthy Skin; control group II = plain Skin Scar; recurrent inguinal hernia group = Skin of recurrent inguinal hernias; each n = 5). Reverse transcription-polymerase chain reaction (RT-PCR) and Northern blot analysis were used to investigate the expression of procollagen type I/- III, MMP-1, and MMP-13 mRNAs. Both ratios of procollagen types I to III mRNAs and collagen types I to III were apparently decreased in the recurrent hernia group compared to those of both control groups (p <0.01). Significant differences were caused by the increase of both procollagen type III mRNA and collagen type III protein synthesis. A concomitant increase of MMP-1 and MMP-13 mRNAs and proteins was also observed in the recurrent hernia group and showed significant differences compared to those of both control groups I and II, respectively (p <0.01). In conclusion, the decreased ratio of collagen types I to III seems not only to be the result of a relative increase in the levels of type III procollagen mRNA but also may be the result of an increase of MMP-1 and MMP-13. The data of the present study strongly suggest recurrent inguinal hernias to be a disease of the collagen matrix and result in a clearer understanding of the underlying pathophysiology and may support specific therapeutic strategies in hernia surgery (e.g., surgical meshes).

  • collagen i iii and matrix metalloproteinases mmp 1 and 13 in the fascia of patients with incisional hernias
    Journal of Investigative Surgery, 2001
    Co-Authors: U Klinge, Z Y Si, H Zheng, V Schumpelick, R S Bhardwaj, B Klosterhalfen
    Abstract:

    The late appearance of incisional hernias several years after laparotomy and the high recurrence rates after operation strongly imply the presence of a disorder of the connective tissue, although a specific defect in patients with incisional hernias has not yet been identified. In the present study we used both immunohistochemistry and Western blot analysis to evaluate the ratio of collagen I and III and the expression of the metalloproteinases (MMP) 1 and 13 in the fascia of patients with incisional or recurrent incisional hernias. Samples of healthy Skin or stable Skin Scar in patients without hernias served as controls. Altogether, our data indicated a significantly decreased ratio of collagen I/III in the fascia of patients with incisional hernias and recurrent incisional hernias. Furthermore, in these patients the expression of MMP-1 was decreased compared to the controls, whereas MMP-13 could not be detected in any fascia sample, with or without hernias present. For the first time, our results give ...