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Masaaki Toyomizu - One of the best experts on this subject based on the ideXlab platform.
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Variation in lysine, threonine, and tryptophan availability in meat and bone meal as estimated by the Slope-Ratio growth assay technique in growing rats.
Animal science journal = Nihon chikusan Gakkaiho, 2020Co-Authors: Hiroyuki Sato, Makoto Miura, Takeshi Fujieda, Marcin Taciak, Motoi Kikusato, Masaaki ToyomizuAbstract:Meat and bone meal (MBM) is an important protein source used in animal feeds. However, as the composition and availability of amino acids (AAs) in MBM fluctuate markedly, it is important to verify the magnitude of these parameters in different MBMs. In this study, the AA compositions of 19 MBMs were analyzed to confirm variations in lysine (Lys), threonine (Thr), and tryptophan (Trp), then which were compared with those of soybean meal (SBM) and fish meal (FM). Instability in Lys, Thr, and Trp availabilities in six MBMs were also considered after estimation using the Slope-Ratio growth assay technique in rats. Variations in AA composition were evaluated using the coefficient of variance (CV: Standard deviation/Mean). CVs for Lys, Thr, and Trp content were 9.40, 11.83, and 18.12 in MBM, 2.71, 2.48, and 3.19 in SBM, and 10.09, 10.44, and 13.47 in FM. Furthermore, means and SDs for Lys, Thr, and Trp availabilities in MBM were 53.3 ± 10.4% (CV: 19.5), 65.9 ± 17.6% (CV: 26.6), and 83.2 ± 11.2% (CV: 13.5), respectively. These results provide the first evidence that variations in MBM AA compositions were 3.5 to 5.7 times higher than those in SBM, but similar to FM, and that the large variation in availability substantially existed.
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application of the Slope Ratio growth assay technique to estimate tryptophan availability in soybean meal fed to young rats
Animal Science Journal, 2019Co-Authors: Hiroyuki Sato, Makoto Miura, Takeshi Fujieda, Masaaki ToyomizuAbstract:The Slope-Ratio assay for rat was used to determine whether tryptophan (Trp) availability in soybean meal (SBM) is affected by the presence of other amino acids (AAs). In a preliminary study, rats were fed graded levels of Trp-supplemented diets to establish the Trp concentRation range over which the weight gain response was linear. This range was found to be from 0.04% to 0.12% Trp. Subsequently, rats were fed basal (0.045% Trp) or Trp-supplemented diets from three different sources: l-Trp alone, SBM, or l-Trp mixed with other AAs to reflect AA levels in the test SBM (AA-mix). Weight gain in rats increased linearly with supplemental Trp intake (p < .05) for all Trp sources. Compared to the Slope achieved with l-Trp alone, the estimated availability of Trp in SBM was 84.4%, while for the AA-mix it was 93.4%. It is evident that the 6.6% reduction in l-Trp availability in AA-mix is due to metabolic costs derived from excess levels of other AAs beside Trp, given that the absorption of crystalline l-Trp in the small intestine is 100%. In conclusion, the Trp availability of SBM was estimated to be around 90.4% (i.e., 84.4/93.4 × 100) after correcting for the effects of the other AAs in SBM.
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Application of the Slope-Ratio growth assay technique to estimate tryptophan availability in soybean meal fed to young rats.
Animal science journal = Nihon chikusan Gakkaiho, 2019Co-Authors: Hiroyuki Sato, Makoto Miura, Takeshi Fujieda, Masaaki ToyomizuAbstract:The Slope-Ratio assay for rat was used to determine whether tryptophan (Trp) availability in soybean meal (SBM) is affected by the presence of other amino acids (AAs). In a preliminary study, rats were fed graded levels of Trp-supplemented diets to establish the Trp concentRation range over which the weight gain response was linear. This range was found to be from 0.04% to 0.12% Trp. Subsequently, rats were fed basal (0.045% Trp) or Trp-supplemented diets from three different sources: l-Trp alone, SBM, or l-Trp mixed with other AAs to reflect AA levels in the test SBM (AA-mix). Weight gain in rats increased linearly with supplemental Trp intake (p
Francisco José Krug - One of the best experts on this subject based on the ideXlab platform.
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Reply to the ‘Comment on “Slope Ratio calibRation for analysis of plant leaves by laser-induced breakdown spectroscopy”’ by Vincenzo Palleschi, JAAS 2020, 35, DOI: C9JA00381A
Journal of Analytical Atomic Spectrometry, 2020Co-Authors: Lidiane Cristina Nunes, Fábio R.p. Rocha, Francisco José KrugAbstract:In response to the comments about the article “Slope Ratio calibRation for analysis of plant leaves by laser-induced breakdown spectroscopy”, this communication clarifies some aspects of this calibRation approach recently proposed for LIBS quantitative analysis.
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reply to the comment on Slope Ratio calibRation for analysis of plant leaves by laser induced breakdown spectroscopy by vincenzo palleschi jaas 2020 35 doi c9ja00381a
Journal of Analytical Atomic Spectrometry, 2020Co-Authors: Lidiane Cristina Nunes, Fábio R.p. Rocha, Francisco José KrugAbstract:In response to the comments about the article “Slope Ratio calibRation for analysis of plant leaves by laser-induced breakdown spectroscopy”, this communication clarifies some aspects of this calibRation approach recently proposed for LIBS quantitative analysis.
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Slope Ratio calibRation for analysis of plant leaves by laser-induced breakdown spectroscopy
Journal of Analytical Atomic Spectrometry, 2019Co-Authors: Lidiane Cristina Nunes, Fábio R.p. Rocha, Francisco José KrugAbstract:A new calibRation strategy based on the increase of the ablated mass with the number of accumulated laser pulses on a single solid calibRation standard is proposed for laser-induced breakdown spectroscopy (LIBS). Under suitable experimental conditions, the emission intensity is directly proportional to the analyte amount in the ablated sample mass, which, in turn, is proportional to the number of laser pulses. Thus, two ablated mass functions are generated by plotting the analyte emission intensities as a function of the number of accumulated laser pulses in the test sample or in the calibRation standard (either a CRM or a reference sample). The analytes mass fractions in the test samples are determined from the Ratio of the corresponding Slopes. The feasibility of this approach, named as Slope Ratio calibRation (SRC-LIBS), was demonstrated by determinations of macro- (Ca, Mg, and P) and micronutrients (B, Mn, Cu, Fe, and Zn) in plant leaves in the form of pressed pellets. In order to minimize drawbacks related to analytes microheterogeneity, the pressed pellets were sampled at 30 different sites for each number of laser pulses. CalibRations performed within 5 and 30 laser pulses per site yielded linear correlation coefficients higher than 0.99 for all the analytes. The accuracy of the proposed calibRation strategy was assessed from the analysis of five CRMs, as well as comparison with the results obtained for sugarcane leaves by ICP OES after microwave-assisted acid digestion, with agreement at the 95% confidence level. Results were more accurate than those determined by the single-point calibRation strategy and by external calibRation with a set of CRMs. The proposed approach is then a fast, simple, and practical calibRation strategy, by requiring only a single standard for quantitative analysis by LIBS.
Hiroyuki Sato - One of the best experts on this subject based on the ideXlab platform.
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Variation in lysine, threonine, and tryptophan availability in meat and bone meal as estimated by the Slope-Ratio growth assay technique in growing rats.
Animal science journal = Nihon chikusan Gakkaiho, 2020Co-Authors: Hiroyuki Sato, Makoto Miura, Takeshi Fujieda, Marcin Taciak, Motoi Kikusato, Masaaki ToyomizuAbstract:Meat and bone meal (MBM) is an important protein source used in animal feeds. However, as the composition and availability of amino acids (AAs) in MBM fluctuate markedly, it is important to verify the magnitude of these parameters in different MBMs. In this study, the AA compositions of 19 MBMs were analyzed to confirm variations in lysine (Lys), threonine (Thr), and tryptophan (Trp), then which were compared with those of soybean meal (SBM) and fish meal (FM). Instability in Lys, Thr, and Trp availabilities in six MBMs were also considered after estimation using the Slope-Ratio growth assay technique in rats. Variations in AA composition were evaluated using the coefficient of variance (CV: Standard deviation/Mean). CVs for Lys, Thr, and Trp content were 9.40, 11.83, and 18.12 in MBM, 2.71, 2.48, and 3.19 in SBM, and 10.09, 10.44, and 13.47 in FM. Furthermore, means and SDs for Lys, Thr, and Trp availabilities in MBM were 53.3 ± 10.4% (CV: 19.5), 65.9 ± 17.6% (CV: 26.6), and 83.2 ± 11.2% (CV: 13.5), respectively. These results provide the first evidence that variations in MBM AA compositions were 3.5 to 5.7 times higher than those in SBM, but similar to FM, and that the large variation in availability substantially existed.
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application of the Slope Ratio growth assay technique to estimate tryptophan availability in soybean meal fed to young rats
Animal Science Journal, 2019Co-Authors: Hiroyuki Sato, Makoto Miura, Takeshi Fujieda, Masaaki ToyomizuAbstract:The Slope-Ratio assay for rat was used to determine whether tryptophan (Trp) availability in soybean meal (SBM) is affected by the presence of other amino acids (AAs). In a preliminary study, rats were fed graded levels of Trp-supplemented diets to establish the Trp concentRation range over which the weight gain response was linear. This range was found to be from 0.04% to 0.12% Trp. Subsequently, rats were fed basal (0.045% Trp) or Trp-supplemented diets from three different sources: l-Trp alone, SBM, or l-Trp mixed with other AAs to reflect AA levels in the test SBM (AA-mix). Weight gain in rats increased linearly with supplemental Trp intake (p < .05) for all Trp sources. Compared to the Slope achieved with l-Trp alone, the estimated availability of Trp in SBM was 84.4%, while for the AA-mix it was 93.4%. It is evident that the 6.6% reduction in l-Trp availability in AA-mix is due to metabolic costs derived from excess levels of other AAs beside Trp, given that the absorption of crystalline l-Trp in the small intestine is 100%. In conclusion, the Trp availability of SBM was estimated to be around 90.4% (i.e., 84.4/93.4 × 100) after correcting for the effects of the other AAs in SBM.
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Application of the Slope-Ratio growth assay technique to estimate tryptophan availability in soybean meal fed to young rats.
Animal science journal = Nihon chikusan Gakkaiho, 2019Co-Authors: Hiroyuki Sato, Makoto Miura, Takeshi Fujieda, Masaaki ToyomizuAbstract:The Slope-Ratio assay for rat was used to determine whether tryptophan (Trp) availability in soybean meal (SBM) is affected by the presence of other amino acids (AAs). In a preliminary study, rats were fed graded levels of Trp-supplemented diets to establish the Trp concentRation range over which the weight gain response was linear. This range was found to be from 0.04% to 0.12% Trp. Subsequently, rats were fed basal (0.045% Trp) or Trp-supplemented diets from three different sources: l-Trp alone, SBM, or l-Trp mixed with other AAs to reflect AA levels in the test SBM (AA-mix). Weight gain in rats increased linearly with supplemental Trp intake (p
Lidiane Cristina Nunes - One of the best experts on this subject based on the ideXlab platform.
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Reply to the ‘Comment on “Slope Ratio calibRation for analysis of plant leaves by laser-induced breakdown spectroscopy”’ by Vincenzo Palleschi, JAAS 2020, 35, DOI: C9JA00381A
Journal of Analytical Atomic Spectrometry, 2020Co-Authors: Lidiane Cristina Nunes, Fábio R.p. Rocha, Francisco José KrugAbstract:In response to the comments about the article “Slope Ratio calibRation for analysis of plant leaves by laser-induced breakdown spectroscopy”, this communication clarifies some aspects of this calibRation approach recently proposed for LIBS quantitative analysis.
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reply to the comment on Slope Ratio calibRation for analysis of plant leaves by laser induced breakdown spectroscopy by vincenzo palleschi jaas 2020 35 doi c9ja00381a
Journal of Analytical Atomic Spectrometry, 2020Co-Authors: Lidiane Cristina Nunes, Fábio R.p. Rocha, Francisco José KrugAbstract:In response to the comments about the article “Slope Ratio calibRation for analysis of plant leaves by laser-induced breakdown spectroscopy”, this communication clarifies some aspects of this calibRation approach recently proposed for LIBS quantitative analysis.
-
Slope Ratio calibRation for analysis of plant leaves by laser-induced breakdown spectroscopy
Journal of Analytical Atomic Spectrometry, 2019Co-Authors: Lidiane Cristina Nunes, Fábio R.p. Rocha, Francisco José KrugAbstract:A new calibRation strategy based on the increase of the ablated mass with the number of accumulated laser pulses on a single solid calibRation standard is proposed for laser-induced breakdown spectroscopy (LIBS). Under suitable experimental conditions, the emission intensity is directly proportional to the analyte amount in the ablated sample mass, which, in turn, is proportional to the number of laser pulses. Thus, two ablated mass functions are generated by plotting the analyte emission intensities as a function of the number of accumulated laser pulses in the test sample or in the calibRation standard (either a CRM or a reference sample). The analytes mass fractions in the test samples are determined from the Ratio of the corresponding Slopes. The feasibility of this approach, named as Slope Ratio calibRation (SRC-LIBS), was demonstrated by determinations of macro- (Ca, Mg, and P) and micronutrients (B, Mn, Cu, Fe, and Zn) in plant leaves in the form of pressed pellets. In order to minimize drawbacks related to analytes microheterogeneity, the pressed pellets were sampled at 30 different sites for each number of laser pulses. CalibRations performed within 5 and 30 laser pulses per site yielded linear correlation coefficients higher than 0.99 for all the analytes. The accuracy of the proposed calibRation strategy was assessed from the analysis of five CRMs, as well as comparison with the results obtained for sugarcane leaves by ICP OES after microwave-assisted acid digestion, with agreement at the 95% confidence level. Results were more accurate than those determined by the single-point calibRation strategy and by external calibRation with a set of CRMs. The proposed approach is then a fast, simple, and practical calibRation strategy, by requiring only a single standard for quantitative analysis by LIBS.
Makoto Miura - One of the best experts on this subject based on the ideXlab platform.
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Variation in lysine, threonine, and tryptophan availability in meat and bone meal as estimated by the Slope-Ratio growth assay technique in growing rats.
Animal science journal = Nihon chikusan Gakkaiho, 2020Co-Authors: Hiroyuki Sato, Makoto Miura, Takeshi Fujieda, Marcin Taciak, Motoi Kikusato, Masaaki ToyomizuAbstract:Meat and bone meal (MBM) is an important protein source used in animal feeds. However, as the composition and availability of amino acids (AAs) in MBM fluctuate markedly, it is important to verify the magnitude of these parameters in different MBMs. In this study, the AA compositions of 19 MBMs were analyzed to confirm variations in lysine (Lys), threonine (Thr), and tryptophan (Trp), then which were compared with those of soybean meal (SBM) and fish meal (FM). Instability in Lys, Thr, and Trp availabilities in six MBMs were also considered after estimation using the Slope-Ratio growth assay technique in rats. Variations in AA composition were evaluated using the coefficient of variance (CV: Standard deviation/Mean). CVs for Lys, Thr, and Trp content were 9.40, 11.83, and 18.12 in MBM, 2.71, 2.48, and 3.19 in SBM, and 10.09, 10.44, and 13.47 in FM. Furthermore, means and SDs for Lys, Thr, and Trp availabilities in MBM were 53.3 ± 10.4% (CV: 19.5), 65.9 ± 17.6% (CV: 26.6), and 83.2 ± 11.2% (CV: 13.5), respectively. These results provide the first evidence that variations in MBM AA compositions were 3.5 to 5.7 times higher than those in SBM, but similar to FM, and that the large variation in availability substantially existed.
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application of the Slope Ratio growth assay technique to estimate tryptophan availability in soybean meal fed to young rats
Animal Science Journal, 2019Co-Authors: Hiroyuki Sato, Makoto Miura, Takeshi Fujieda, Masaaki ToyomizuAbstract:The Slope-Ratio assay for rat was used to determine whether tryptophan (Trp) availability in soybean meal (SBM) is affected by the presence of other amino acids (AAs). In a preliminary study, rats were fed graded levels of Trp-supplemented diets to establish the Trp concentRation range over which the weight gain response was linear. This range was found to be from 0.04% to 0.12% Trp. Subsequently, rats were fed basal (0.045% Trp) or Trp-supplemented diets from three different sources: l-Trp alone, SBM, or l-Trp mixed with other AAs to reflect AA levels in the test SBM (AA-mix). Weight gain in rats increased linearly with supplemental Trp intake (p < .05) for all Trp sources. Compared to the Slope achieved with l-Trp alone, the estimated availability of Trp in SBM was 84.4%, while for the AA-mix it was 93.4%. It is evident that the 6.6% reduction in l-Trp availability in AA-mix is due to metabolic costs derived from excess levels of other AAs beside Trp, given that the absorption of crystalline l-Trp in the small intestine is 100%. In conclusion, the Trp availability of SBM was estimated to be around 90.4% (i.e., 84.4/93.4 × 100) after correcting for the effects of the other AAs in SBM.
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Application of the Slope-Ratio growth assay technique to estimate tryptophan availability in soybean meal fed to young rats.
Animal science journal = Nihon chikusan Gakkaiho, 2019Co-Authors: Hiroyuki Sato, Makoto Miura, Takeshi Fujieda, Masaaki ToyomizuAbstract:The Slope-Ratio assay for rat was used to determine whether tryptophan (Trp) availability in soybean meal (SBM) is affected by the presence of other amino acids (AAs). In a preliminary study, rats were fed graded levels of Trp-supplemented diets to establish the Trp concentRation range over which the weight gain response was linear. This range was found to be from 0.04% to 0.12% Trp. Subsequently, rats were fed basal (0.045% Trp) or Trp-supplemented diets from three different sources: l-Trp alone, SBM, or l-Trp mixed with other AAs to reflect AA levels in the test SBM (AA-mix). Weight gain in rats increased linearly with supplemental Trp intake (p