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Yutaka Oono - One of the best experts on this subject based on the ideXlab platform.
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a gene encoding Small Acidic Protein 2 potentially mediates the response to synthetic auxin 2 4 dichlorophenoxyacetic acid in arabidopsis thaliana
Journal of Plant Physiology, 2009Co-Authors: Akari Nakasone, Tomohiro Kiyosue, Issay Narumi, Maki Kawaiyamada, Hirofumi Uchimiya, Yutaka OonoAbstract:Summary The Small Acidic Protein 2 ( SMAP2 ) gene is a paralogue of the SMAP1 gene that mediates the response to the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) in the root of Arabidopsis thaliana . Their encoded Proteins, SMAP1 and SMAP2, are similar in calculated molecular weight and isoelectric point, and in having a highly conserved phenylalanine and aspartic acid-rich domain. RNA expression analysis showed that SMAP1 mRNA is present throughout the plant body while SMAP2 mRNA is restricted to siliques and anthers. Over-expression of the SMAP2 gene, as well as SMAP1 , by 35S cauliflower mosaic virus promoter restored sensitivity to 2,4-D in the 2,4-D-resistant mutant, aar1 , which is defective in SMAP1 function. The results suggest that SMAP2 has an ability to mediate the 2,4-D response and is expressed only in restricted tissues.
Akari Nakasone - One of the best experts on this subject based on the ideXlab platform.
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a gene encoding Small Acidic Protein 2 potentially mediates the response to synthetic auxin 2 4 dichlorophenoxyacetic acid in arabidopsis thaliana
Journal of Plant Physiology, 2009Co-Authors: Akari Nakasone, Tomohiro Kiyosue, Issay Narumi, Maki Kawaiyamada, Hirofumi Uchimiya, Yutaka OonoAbstract:Summary The Small Acidic Protein 2 ( SMAP2 ) gene is a paralogue of the SMAP1 gene that mediates the response to the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) in the root of Arabidopsis thaliana . Their encoded Proteins, SMAP1 and SMAP2, are similar in calculated molecular weight and isoelectric point, and in having a highly conserved phenylalanine and aspartic acid-rich domain. RNA expression analysis showed that SMAP1 mRNA is present throughout the plant body while SMAP2 mRNA is restricted to siliques and anthers. Over-expression of the SMAP2 gene, as well as SMAP1 , by 35S cauliflower mosaic virus promoter restored sensitivity to 2,4-D in the 2,4-D-resistant mutant, aar1 , which is defective in SMAP1 function. The results suggest that SMAP2 has an ability to mediate the 2,4-D response and is expressed only in restricted tissues.
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a Small Acidic Protein 1 smap1 mediates responses of the arabidopsis root to the synthetic auxin 2 4 dichlorophenoxyacetic acid
Plant Journal, 2006Co-Authors: Abidur Rahman, Akari Nakasone, Hirofumi Uchimiya, Tory Chhun, Chiharu Ooura, Kamal Kanti Biswas, Seiji Tsurumi, Tobias I BaskinAbstract:*† ‡ § Summary 2,4-dichlorophenoxyacetic acid (2,4-D), a chemical analogue of indole-3-acetic acid (IAA), is widely used as a growth regulator and exogenous source of auxin. Because 2,4-D evokes physiological and molecular responses similar to those evoked by IAA, it is believed that they share a common response pathway. Here, we show that a mutant, antiauxin resistant1 (aar1), identified in a screen for resistance to the anti-auxin p-chlorophenoxyisobutyric acid (PCIB), is resistant to 2,4-D, yet nevertheless responds like the wild-type to IAA and 1-napthaleneacetic acid in root elongation and lateral root induction assays. That the aar1 mutation alters 2,4-D responsiveness specifically was confirmed by analysis of GUS expression in the DR5:GUS and HS:AXR3NT-GUS backgrounds, as well as by real-time PCR quantification of IAA11 expression. The two characterized aar1 alleles both harbor multi-gene deletions; however, 2,4-D responsiveness was restored by transformation with one of the genes missing in both alleles, and the 2,4-D-resistant phenotype was reproduced by decreasing the expression of the same gene in the wild-type using an RNAi construct. The gene encodes a Small, Acidic Protein (SMAP1) with unknown function and present in plants, animals and invertebrates but not in fungi or prokaryotes. Taken together, these results suggest that SMAP1 is a regulatory component that mediates responses to 2,4-D, and that responses to 2,4-D and IAA are partially distinct.
Maki Kawaiyamada - One of the best experts on this subject based on the ideXlab platform.
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a gene encoding Small Acidic Protein 2 potentially mediates the response to synthetic auxin 2 4 dichlorophenoxyacetic acid in arabidopsis thaliana
Journal of Plant Physiology, 2009Co-Authors: Akari Nakasone, Tomohiro Kiyosue, Issay Narumi, Maki Kawaiyamada, Hirofumi Uchimiya, Yutaka OonoAbstract:Summary The Small Acidic Protein 2 ( SMAP2 ) gene is a paralogue of the SMAP1 gene that mediates the response to the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) in the root of Arabidopsis thaliana . Their encoded Proteins, SMAP1 and SMAP2, are similar in calculated molecular weight and isoelectric point, and in having a highly conserved phenylalanine and aspartic acid-rich domain. RNA expression analysis showed that SMAP1 mRNA is present throughout the plant body while SMAP2 mRNA is restricted to siliques and anthers. Over-expression of the SMAP2 gene, as well as SMAP1 , by 35S cauliflower mosaic virus promoter restored sensitivity to 2,4-D in the 2,4-D-resistant mutant, aar1 , which is defective in SMAP1 function. The results suggest that SMAP2 has an ability to mediate the 2,4-D response and is expressed only in restricted tissues.
Hirofumi Uchimiya - One of the best experts on this subject based on the ideXlab platform.
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a gene encoding Small Acidic Protein 2 potentially mediates the response to synthetic auxin 2 4 dichlorophenoxyacetic acid in arabidopsis thaliana
Journal of Plant Physiology, 2009Co-Authors: Akari Nakasone, Tomohiro Kiyosue, Issay Narumi, Maki Kawaiyamada, Hirofumi Uchimiya, Yutaka OonoAbstract:Summary The Small Acidic Protein 2 ( SMAP2 ) gene is a paralogue of the SMAP1 gene that mediates the response to the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) in the root of Arabidopsis thaliana . Their encoded Proteins, SMAP1 and SMAP2, are similar in calculated molecular weight and isoelectric point, and in having a highly conserved phenylalanine and aspartic acid-rich domain. RNA expression analysis showed that SMAP1 mRNA is present throughout the plant body while SMAP2 mRNA is restricted to siliques and anthers. Over-expression of the SMAP2 gene, as well as SMAP1 , by 35S cauliflower mosaic virus promoter restored sensitivity to 2,4-D in the 2,4-D-resistant mutant, aar1 , which is defective in SMAP1 function. The results suggest that SMAP2 has an ability to mediate the 2,4-D response and is expressed only in restricted tissues.
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a Small Acidic Protein 1 smap1 mediates responses of the arabidopsis root to the synthetic auxin 2 4 dichlorophenoxyacetic acid
Plant Journal, 2006Co-Authors: Abidur Rahman, Akari Nakasone, Hirofumi Uchimiya, Tory Chhun, Chiharu Ooura, Kamal Kanti Biswas, Seiji Tsurumi, Tobias I BaskinAbstract:*† ‡ § Summary 2,4-dichlorophenoxyacetic acid (2,4-D), a chemical analogue of indole-3-acetic acid (IAA), is widely used as a growth regulator and exogenous source of auxin. Because 2,4-D evokes physiological and molecular responses similar to those evoked by IAA, it is believed that they share a common response pathway. Here, we show that a mutant, antiauxin resistant1 (aar1), identified in a screen for resistance to the anti-auxin p-chlorophenoxyisobutyric acid (PCIB), is resistant to 2,4-D, yet nevertheless responds like the wild-type to IAA and 1-napthaleneacetic acid in root elongation and lateral root induction assays. That the aar1 mutation alters 2,4-D responsiveness specifically was confirmed by analysis of GUS expression in the DR5:GUS and HS:AXR3NT-GUS backgrounds, as well as by real-time PCR quantification of IAA11 expression. The two characterized aar1 alleles both harbor multi-gene deletions; however, 2,4-D responsiveness was restored by transformation with one of the genes missing in both alleles, and the 2,4-D-resistant phenotype was reproduced by decreasing the expression of the same gene in the wild-type using an RNAi construct. The gene encodes a Small, Acidic Protein (SMAP1) with unknown function and present in plants, animals and invertebrates but not in fungi or prokaryotes. Taken together, these results suggest that SMAP1 is a regulatory component that mediates responses to 2,4-D, and that responses to 2,4-D and IAA are partially distinct.
Issay Narumi - One of the best experts on this subject based on the ideXlab platform.
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a gene encoding Small Acidic Protein 2 potentially mediates the response to synthetic auxin 2 4 dichlorophenoxyacetic acid in arabidopsis thaliana
Journal of Plant Physiology, 2009Co-Authors: Akari Nakasone, Tomohiro Kiyosue, Issay Narumi, Maki Kawaiyamada, Hirofumi Uchimiya, Yutaka OonoAbstract:Summary The Small Acidic Protein 2 ( SMAP2 ) gene is a paralogue of the SMAP1 gene that mediates the response to the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) in the root of Arabidopsis thaliana . Their encoded Proteins, SMAP1 and SMAP2, are similar in calculated molecular weight and isoelectric point, and in having a highly conserved phenylalanine and aspartic acid-rich domain. RNA expression analysis showed that SMAP1 mRNA is present throughout the plant body while SMAP2 mRNA is restricted to siliques and anthers. Over-expression of the SMAP2 gene, as well as SMAP1 , by 35S cauliflower mosaic virus promoter restored sensitivity to 2,4-D in the 2,4-D-resistant mutant, aar1 , which is defective in SMAP1 function. The results suggest that SMAP2 has an ability to mediate the 2,4-D response and is expressed only in restricted tissues.