The Experts below are selected from a list of 315 Experts worldwide ranked by ideXlab platform
Theresa Welch Fossum - One of the best experts on this subject based on the ideXlab platform.
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Small Animal Surgery, 4th Edition
2012Co-Authors: Theresa Welch FossumAbstract:Small Animal Surgery, 4th Edition - Libros de Medicina - Medicina y Cirugia Animal - 149,15
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Comprar Small Animal Surgery, 4th Edition | Theresa Welch Fossum | 9780323077620a | Mosby
2012Co-Authors: Theresa Welch FossumAbstract:Tienda online donde Comprar Small Animal Surgery, 4th Edition al precio 156,61 € de Theresa Welch Fossum, tienda de Libros de Medicina, Libros de Veterinaria - Medicina y Cirugia Animal
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comprar Small Animal Surgery 4th edition theresa welch fossum 9780323077620a mosby
2012Co-Authors: Theresa Welch FossumAbstract:Tienda online donde Comprar Small Animal Surgery, 4th Edition al precio 156,61 € de Theresa Welch Fossum, tienda de Libros de Medicina, Libros de Veterinaria - Medicina y Cirugia Animal
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Small Animal Surgery
1997Co-Authors: Theresa Welch FossumAbstract:Part I: General Surgical Principles * Principles of Surgical Asepsis * Sterilization and Disinfection * Surgical Facilities, Equipment, and Personnel and Care and Maintenance of the Surgical Environment * Preoperative and Intraoperative Care of the Surgical Patient * Preparation of the Operative Site * Preparation of the Surgical Team * Surgical Instrumentation * Biomaterials, Suturing, and Suture Patterns * Surgical Infections and Antibiotic Selection * Nutritional Management of the Surgical Patient * Fundamentals of Physical Rehabilitation * Perioperative Multimodal Analgesic Therapy * Principles of Minimally Invasive Surgery * Regenerative Medicine and Stem Cell Therapy * Advanced Imaging for Surgeons Part II: Soft Tissue Surgery * Surgery of the Integumentary System * Surgery of the Eye * Surgery of the Ear * Surgery of the Abdominal Cavity * Surgery of the Digestive System * Surgery of the Liver * Surgery of the Extrahepatic Biliary System * Surgery of the Endocrine System * Surgery of the Hemolymphatic System * Surgery of the Kidney and Ureter * Surgery of the Bladder and Urethra * Surgery of the Reproductive and Genital Systems * Surgery of the Cardiovascular System * Surgery of the Upper Respiratory System * Surgery of the Lower Respiratory System: Lungs and Thoracic Wall * Surgery of the Lower Respiratory System: Pleural Cavity and Diaphragm Part III: Orthopedics * Fundamentals of Orthopedic Surgery and Fracture Management * Management of Specific Fractures * Diseases of the Joints * Management of Muscle and Tendon Injury or Disease * Other Diseases of Bones and Joints Part IV: NeuroSurgery * Neurodiagnostic Overview for the Small Animal Surgeon * Neurologic Examination and Relevant Neuroanatomy * Surgery of the Brain * Surgery of the Cervical Spine * Surgery of the Thoracolumbar Spine * Surgery of the Cauda Equina * Nonsurgical Disorders of the Brain and Spine * Peripheral Nervous System Disorders
Shinichi Nakagawa - One of the best experts on this subject based on the ideXlab platform.
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Creation of CRISPR-based germline-genome-engineered mice without ex vivo handling of zygotes by i-GONAD
Nature Protocols, 2019Co-Authors: Channabasavaiah B. Gurumurthy, Masahiro Sato, Ayaka Nakamura, Masafumi Inui, Natsuko Kawano, Sanae Ogiwara, Shuji Takabayashi, Makoto Matsuyama, Md Atiqul Islam, Shinichi NakagawaAbstract:Methods to create genetically engineered mice involve three major steps: harvesting embryos from one set of females, microinjection of reagents into embryos ex vivo and their surgical transfer to another set of females. Although tedious, these methods have been used for more than three decades to create mouse models. We recently developed a method named GONAD (genome editing via oviductal nucleic acids delivery), which bypasses these steps. GONAD involves injection of CRISPR components (Cas9 mRNA and guide RNA (gRNA)) into the oviducts of pregnant females 1.5 d post conception, followed by in vivo electroporation to deliver the components into the zygotes in situ. Using GONAD, we demonstrated that target genes can be disrupted and analyzed at different stages of mouse embryonic development. Subsequently, we developed improved GONAD ( i -GONAD) by delivering CRISPR ribonucleoproteins (RNPs; Cas9 protein or Cpf1 protein and gRNA) into day-0.7 pregnant mice, which made it suitable for routine generation of knockout and large-deletion mouse models. i -GONAD can also generate knock-in models containing up to 1-kb inserts when single-stranded DNA (ssDNA) repair templates are supplied. i -GONAD offers other advantages: it does not require vasectomized males and pseudo-pregnant females, the females used for i -GONAD are not sacrificed and can be used for other experiments, it can be easily adopted in laboratories lacking sophisticated microinjection equipment, and can be implemented by researchers skilled in Small-Animal Surgery but lacking embryo-handling skills. Here, we provide a step-by-step protocol for establishing the i -GONAD method. The protocol takes ∼6 weeks to generate the founder mice. This protocol describes procedures for generating genome-edited mouse models by injecting CRISPR reagents into oviducts of pregnant females and subsequently electroporating the reagents into zygotes in situ, thus bypassing cumbersome ex vivo handling of embryos.
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Creation of CRISPR-based germline-genome-engineered mice without ex vivo handling of zygotes by i-GONAD
Nature protocols, 2019Co-Authors: Channabasavaiah B. Gurumurthy, Masahiro Sato, Ayaka Nakamura, Masafumi Inui, Natsuko Kawano, Atiqul Islam, Sanae Ogiwara, Shuji Takabayashi, Makoto Matsuyama, Shinichi NakagawaAbstract:Methods to create genetically engineered mice involve three major steps: harvesting embryos from one set of females, microinjection of reagents into embryos ex vivo and their surgical transfer to another set of females. Although tedious, these methods have been used for more than three decades to create mouse models. We recently developed a method named GONAD (genome editing via oviductal nucleic acids delivery), which bypasses these steps. GONAD involves injection of CRISPR components (Cas9 mRNA and guide RNA (gRNA)) into the oviducts of pregnant females 1.5 d post conception, followed by in vivo electroporation to deliver the components into the zygotes in situ. Using GONAD, we demonstrated that target genes can be disrupted and analyzed at different stages of mouse embryonic development. Subsequently, we developed improved GONAD (i-GONAD) by delivering CRISPR ribonucleoproteins (RNPs; Cas9 protein or Cpf1 protein and gRNA) into day-0.7 pregnant mice, which made it suitable for routine generation of knockout and large-deletion mouse models. i-GONAD can also generate knock-in models containing up to 1-kb inserts when single-stranded DNA (ssDNA) repair templates are supplied. i-GONAD offers other advantages: it does not require vasectomized males and pseudo-pregnant females, the females used for i-GONAD are not sacrificed and can be used for other experiments, it can be easily adopted in laboratories lacking sophisticated microinjection equipment, and can be implemented by researchers skilled in Small-Animal Surgery but lacking embryo-handling skills. Here, we provide a step-by-step protocol for establishing the i-GONAD method. The protocol takes ∼6 weeks to generate the founder mice.
Channabasavaiah B. Gurumurthy - One of the best experts on this subject based on the ideXlab platform.
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Creation of CRISPR-based germline-genome-engineered mice without ex vivo handling of zygotes by i-GONAD
Nature Protocols, 2019Co-Authors: Channabasavaiah B. Gurumurthy, Masahiro Sato, Ayaka Nakamura, Masafumi Inui, Natsuko Kawano, Sanae Ogiwara, Shuji Takabayashi, Makoto Matsuyama, Md Atiqul Islam, Shinichi NakagawaAbstract:Methods to create genetically engineered mice involve three major steps: harvesting embryos from one set of females, microinjection of reagents into embryos ex vivo and their surgical transfer to another set of females. Although tedious, these methods have been used for more than three decades to create mouse models. We recently developed a method named GONAD (genome editing via oviductal nucleic acids delivery), which bypasses these steps. GONAD involves injection of CRISPR components (Cas9 mRNA and guide RNA (gRNA)) into the oviducts of pregnant females 1.5 d post conception, followed by in vivo electroporation to deliver the components into the zygotes in situ. Using GONAD, we demonstrated that target genes can be disrupted and analyzed at different stages of mouse embryonic development. Subsequently, we developed improved GONAD ( i -GONAD) by delivering CRISPR ribonucleoproteins (RNPs; Cas9 protein or Cpf1 protein and gRNA) into day-0.7 pregnant mice, which made it suitable for routine generation of knockout and large-deletion mouse models. i -GONAD can also generate knock-in models containing up to 1-kb inserts when single-stranded DNA (ssDNA) repair templates are supplied. i -GONAD offers other advantages: it does not require vasectomized males and pseudo-pregnant females, the females used for i -GONAD are not sacrificed and can be used for other experiments, it can be easily adopted in laboratories lacking sophisticated microinjection equipment, and can be implemented by researchers skilled in Small-Animal Surgery but lacking embryo-handling skills. Here, we provide a step-by-step protocol for establishing the i -GONAD method. The protocol takes ∼6 weeks to generate the founder mice. This protocol describes procedures for generating genome-edited mouse models by injecting CRISPR reagents into oviducts of pregnant females and subsequently electroporating the reagents into zygotes in situ, thus bypassing cumbersome ex vivo handling of embryos.
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Creation of CRISPR-based germline-genome-engineered mice without ex vivo handling of zygotes by i-GONAD
Nature protocols, 2019Co-Authors: Channabasavaiah B. Gurumurthy, Masahiro Sato, Ayaka Nakamura, Masafumi Inui, Natsuko Kawano, Atiqul Islam, Sanae Ogiwara, Shuji Takabayashi, Makoto Matsuyama, Shinichi NakagawaAbstract:Methods to create genetically engineered mice involve three major steps: harvesting embryos from one set of females, microinjection of reagents into embryos ex vivo and their surgical transfer to another set of females. Although tedious, these methods have been used for more than three decades to create mouse models. We recently developed a method named GONAD (genome editing via oviductal nucleic acids delivery), which bypasses these steps. GONAD involves injection of CRISPR components (Cas9 mRNA and guide RNA (gRNA)) into the oviducts of pregnant females 1.5 d post conception, followed by in vivo electroporation to deliver the components into the zygotes in situ. Using GONAD, we demonstrated that target genes can be disrupted and analyzed at different stages of mouse embryonic development. Subsequently, we developed improved GONAD (i-GONAD) by delivering CRISPR ribonucleoproteins (RNPs; Cas9 protein or Cpf1 protein and gRNA) into day-0.7 pregnant mice, which made it suitable for routine generation of knockout and large-deletion mouse models. i-GONAD can also generate knock-in models containing up to 1-kb inserts when single-stranded DNA (ssDNA) repair templates are supplied. i-GONAD offers other advantages: it does not require vasectomized males and pseudo-pregnant females, the females used for i-GONAD are not sacrificed and can be used for other experiments, it can be easily adopted in laboratories lacking sophisticated microinjection equipment, and can be implemented by researchers skilled in Small-Animal Surgery but lacking embryo-handling skills. Here, we provide a step-by-step protocol for establishing the i-GONAD method. The protocol takes ∼6 weeks to generate the founder mice.
Ameet Singh - One of the best experts on this subject based on the ideXlab platform.
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Use of a surgical safety checklist after implementation in an academic veterinary hospital
Veterinary surgery : VS, 2020Co-Authors: William T. G. Hawker, Thomas W.g. Gibson, Ameet Singh, Michelle A. Giuffrida, J. Scott WeeseAbstract:OBJECTIVE To determine the use and barriers to uptake of a surgical safety checklist (SSC) after implementation in a veterinary teaching hospital. STUDY DESIGN Voluntary online survey and retrospective study. SAMPLE POPULATION All personnel actively involved in the Ontario Veterinary College Health Sciences Centre Small Animal Surgery service between October 2, 2018 and June 28, 2019. METHODS Surgical case logs and electronically initiated SSC were reviewed to calculate checklist use. The sample population was surveyed to identify factors and barriers associated with use of the SSC. Participants were allowed 1 month to respond, and five reminder emails were sent. RESULTS Forth-one of 50 (82%) participants completed the survey. The SSC was used in 374 of 784 (47.7%) surgeries. Use rates declined over sequential three-month intervals (P
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Adherence of methicillin-resistant Staphylococcus pseudintermedius to suture materials commonly used in Small Animal Surgery
American journal of veterinary research, 2016Co-Authors: Shauna Morrison, Ameet Singh, Joyce Rousseau, J. Scott WeeseAbstract:OBJECTIVE To evaluate adherence of methicillin-resistant Staphylococcus pseudintermedius (MRSP) to 5 suture materials commonly used in Small Animal Surgery. SAMPLE 10 epidemiologically unrelated MRSP isolates (obtained from dogs with clinical infections) that had strong biofilm-forming ability and 5 types of suture. PROCEDURES The 5 types of suture evaluated were monofilament polyglecaprone 25, monofilament polydioxanone, triclosan-coated (TC)–monofilament polydioxanone, braided polyglactin 910, and barbed monofilament polydioxanone. Suture segments were incubated in standard suspensions of MRSP for 2 minutes. Segments were then placed in tryptone soy broth and incubated overnight. After incubation, segments were rinsed with PBS solution and sonicated to dislodge adherent bacteria. Resulting suspensions were used to create serial dilutions that were plated, incubated overnight, and counted the following day. Bacterial adherence to 1 segment of each suture type was assessed by use of scanning electron micr...
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Investigation of Incidence and Risk Factors for Surgical Glove Perforation in Small Animal Surgery
Veterinary Surgery, 2014Co-Authors: Galina M. Hayes, Deborah Reynolds, Michelle Oblak, Thomas W.g. Gibson, Alim Nazarali, Ameet Singh, Brigitte A. Brisson, N.m.m. Moens, Cate DeweyAbstract:Objective To identify incidence and risk factors for surgical glove perforation in Small Animal Surgery. Study Design Observational cohort study. Sample Population Surgical gloves (n = 2132) worn in 363 surgical procedures. Methods All gloves worn by operative personnel were assessed for perforation at end-procedure using a water leak test. Putative risk factors were recorded by a surgical team member. Associations between risk factors and perforation were assessed using multivariable multi-level random-effects logistic regression models to control for hierarchical data structure. Results At least 1 glove perforation occurred in 26.2% of procedures. Identified risk factors for glove perforation included increased surgical duration (Surgery >1 hour OR = 1.79, 95% CI = 1.12–2.86), performing orthopedic procedures (OR = 1.88; 95% CI = 1.23–2.88), any procedure using powered instruments (OR = 1.93; 95% CI = 1.21–3.09) or surgical wire (OR = 3.02; 95% CI = 1.50–6.05), use of polyisoprene as a glove material (OR = 1.59, 95% CI = 1.05–2.39), and operative role as primary surgeon (OR = 2.01; 95% CI = 1.35–2.98). The ability of the wearer to detect perforations intraoperatively was poor, with a sensitivity of 30.8%. Conclusions There is a high incidence of unrecognized glove perforations in Small Animal Surgery.
J. Scott Weese - One of the best experts on this subject based on the ideXlab platform.
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Use of a surgical safety checklist after implementation in an academic veterinary hospital
Veterinary surgery : VS, 2020Co-Authors: William T. G. Hawker, Thomas W.g. Gibson, Ameet Singh, Michelle A. Giuffrida, J. Scott WeeseAbstract:OBJECTIVE To determine the use and barriers to uptake of a surgical safety checklist (SSC) after implementation in a veterinary teaching hospital. STUDY DESIGN Voluntary online survey and retrospective study. SAMPLE POPULATION All personnel actively involved in the Ontario Veterinary College Health Sciences Centre Small Animal Surgery service between October 2, 2018 and June 28, 2019. METHODS Surgical case logs and electronically initiated SSC were reviewed to calculate checklist use. The sample population was surveyed to identify factors and barriers associated with use of the SSC. Participants were allowed 1 month to respond, and five reminder emails were sent. RESULTS Forth-one of 50 (82%) participants completed the survey. The SSC was used in 374 of 784 (47.7%) surgeries. Use rates declined over sequential three-month intervals (P
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Adherence of methicillin-resistant Staphylococcus pseudintermedius to suture materials commonly used in Small Animal Surgery
American journal of veterinary research, 2016Co-Authors: Shauna Morrison, Ameet Singh, Joyce Rousseau, J. Scott WeeseAbstract:OBJECTIVE To evaluate adherence of methicillin-resistant Staphylococcus pseudintermedius (MRSP) to 5 suture materials commonly used in Small Animal Surgery. SAMPLE 10 epidemiologically unrelated MRSP isolates (obtained from dogs with clinical infections) that had strong biofilm-forming ability and 5 types of suture. PROCEDURES The 5 types of suture evaluated were monofilament polyglecaprone 25, monofilament polydioxanone, triclosan-coated (TC)–monofilament polydioxanone, braided polyglactin 910, and barbed monofilament polydioxanone. Suture segments were incubated in standard suspensions of MRSP for 2 minutes. Segments were then placed in tryptone soy broth and incubated overnight. After incubation, segments were rinsed with PBS solution and sonicated to dislodge adherent bacteria. Resulting suspensions were used to create serial dilutions that were plated, incubated overnight, and counted the following day. Bacterial adherence to 1 segment of each suture type was assessed by use of scanning electron micr...