The Experts below are selected from a list of 180 Experts worldwide ranked by ideXlab platform
Fumiaki Itoi - One of the best experts on this subject based on the ideXlab platform.
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clinical outcomes after ivf or icsi using human blastocysts derived from oocytes containing aggregates of Smooth Endoplasmic Reticulum
Reproductive Biomedicine Online, 2017Co-Authors: Fumiaki Itoi, Yukiko Asano, Masashi Shimizu, Hiroyuki Honnma, Rika Nagai, Kanako Saitou, Yasutaka MurataAbstract:In this study the clinical and neo-natal outcomes after transfer of blastocysts derived from oocytes containing aggregates of Smooth Endoplasmic Reticulum (SER) were compared between IVF and intracytoplasmic sperm injection (ICSI) cycles. Clinical and neo-natal outcomes of blastocysts in cycles with at least one SER metaphase II oocyte (SER + MII; SER + cycles) did not significantly differ between the two insemination methods. When SER + MII were cultured to day 5/6, fertilization, embryo cleavage and blastocyst rates were not significantly different between IVF and ICSI cycles. In vitrified-warmed blastocyst transfer cycles, the clinical pregnancy rates from SER + MII in IVF and ICSI did not significantly differ. In this study, 52 blastocysts (27 IVF and 25 ICSI) derived from SER + MII were transferred, yielding 15 newborns (5 IVF and 10 ICSI) and no malformations. Moreover, 300 blastocysts (175 IVF and 125 ICSI) derived from SER-MII were transferred, yielding 55 newborns (24 IVF and 31 ICSI cycles). Thus, blastocysts derived from SER + cycles exhibited an acceptable ongoing pregnancy rate after IVF (n = 125) or ICSI (n = 117) cycles. In conclusion, blastocysts from SER + MII in both IVF and ICSI cycles yield adequate ongoing pregnancy rates with neo-natal outcomes that do not differ from SER-MII.
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embryological outcomes in cycles with human oocytes containing large tubular Smooth Endoplasmic Reticulum clusters after conventional in vitro fertilization
Gynecological Endocrinology, 2016Co-Authors: Fumiaki Itoi, Yukiko Asano, Masashi Shimizu, Hiroyuki Honnma, Yasutaka MurataAbstract:There have been no studies analyzing the effect of large aggregates of tubular Smooth Endoplasmic Reticulum (aSERT) after conventional in vitro fertilization (cIVF). The aim of this study was to investigate whether aSERT can be identified after cIVF and the association between the embryological outcomes of oocytes in cycles with aSERT. This is a retrospective study examining embryological data from cIVF cycles showing the presence of aSERT in oocytes 5-6 h after cIVF. To evaluate embryo quality, cIVF cycles with at least one aSERT-metaphase II (MII) oocyte observed (cycles with aSERT) were compared to cycles with normal-MII oocytes (control cycles). Among the 4098 MII oocytes observed in 579 cycles, aSERT was detected in 100 MII oocytes in 51 cycles (8.8%). The fertilization rate, the rate of embryo development on day 3 and day 5-6 did not significantly differ between cycles with aSERT and control group. However, aSERT-MII oocytes had lower rates for both blastocysts and good quality blastocysts (p < 0.05). aSERT can be detected in the cytoplasm by removing the cumulus cell 5 h after cIVF. However, aSERT-MII oocytes do not affect other normal-MII oocytes in cycles with aSERT.
Mário Sousa - One of the best experts on this subject based on the ideXlab platform.
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ultrastructure of tubular Smooth Endoplasmic Reticulum aggregates in human metaphase ii oocytes and clinical implications
Fertility and Sterility, 2011Co-Authors: Mariana Cunha, A. Luis, J. Silva, Alberto Barros, Cristiano Oliveira, Jose Teixeira Da Silva, Mário SousaAbstract:Objective To compare demographic, embryologic, pregnancy, and newborn outcomes after intracytoplasmic sperm injection (ICSI) cycles with or without mature oocytes (metaphase II [MII]) showing visible aggregates of tubular Smooth Endoplasmic Reticulum (aSERT) and to describe the ultrastructure of this dysmorphism. Design Retrospective study. Setting Private fertility center and university cell biology and genetics departments. Patient(s) There were 721 ICSI cycles, 520 carrying morphologically normal MII (control group) and 60 containing aSERT-MII (study group). Intervention(s) None. Main Outcome Measure(s) Embryologic and clinical and live birth outcomes, including malformations and ultrastructural characterization of aSERT-MII. Result(s) Compared with the control group there was a significant decrease in the fertilization, embryo cleavage, and blastocyst rates in the study group. The only child born after transfer of embryos derived from aSERT-MII presented a major cardiovascular malformation. Ultrastructurally, large aSERT were surrounded by abnormal-shaped mitochondria and clusters of small dense bodies formed by very small vesicles, and they had curvilinear dense tubules in the interior. The same pathology was observed in small peripheral aSERT. Conclusion(s) The presence of large aSERT, showing attainment of the periphery, demonstrated that the cytoplasm is pathologic. The compromised embryo development and implantation was associated with decreased clinical outcomes and newborn malformations. Therefore, oocytes with large aSERT should not be used for embryo transfer.
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Stereological Analysis of Mitochondria and Smooth Endoplasmic Reticulum Distribution in Human Oocytes at Prophase I
Microscopy and Microanalysis, 2008Co-Authors: A. Luis, Eduardo Rocha, Elsa Oliveira, J. Silva, Alberto Barros, Mário SousaAbstract:The structure of human oocytes at the different maturation stages is known based on descriptive studies of its ultrastructural composition. The tridimensional quantitative ultrastructural analysis of human oocytes would be important for understanding folliculogenesis and establishing adequate methods of in vitro oocyte maturation. In this study, stereological methods were applied to quantify the distribution of mitochondria (M) and Smooth Endoplasmic Reticulum (SER) in oocytes at the germinal vesicle (VG) stage of prophase I.
Yasutaka Murata - One of the best experts on this subject based on the ideXlab platform.
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clinical outcomes after ivf or icsi using human blastocysts derived from oocytes containing aggregates of Smooth Endoplasmic Reticulum
Reproductive Biomedicine Online, 2017Co-Authors: Fumiaki Itoi, Yukiko Asano, Masashi Shimizu, Hiroyuki Honnma, Rika Nagai, Kanako Saitou, Yasutaka MurataAbstract:In this study the clinical and neo-natal outcomes after transfer of blastocysts derived from oocytes containing aggregates of Smooth Endoplasmic Reticulum (SER) were compared between IVF and intracytoplasmic sperm injection (ICSI) cycles. Clinical and neo-natal outcomes of blastocysts in cycles with at least one SER metaphase II oocyte (SER + MII; SER + cycles) did not significantly differ between the two insemination methods. When SER + MII were cultured to day 5/6, fertilization, embryo cleavage and blastocyst rates were not significantly different between IVF and ICSI cycles. In vitrified-warmed blastocyst transfer cycles, the clinical pregnancy rates from SER + MII in IVF and ICSI did not significantly differ. In this study, 52 blastocysts (27 IVF and 25 ICSI) derived from SER + MII were transferred, yielding 15 newborns (5 IVF and 10 ICSI) and no malformations. Moreover, 300 blastocysts (175 IVF and 125 ICSI) derived from SER-MII were transferred, yielding 55 newborns (24 IVF and 31 ICSI cycles). Thus, blastocysts derived from SER + cycles exhibited an acceptable ongoing pregnancy rate after IVF (n = 125) or ICSI (n = 117) cycles. In conclusion, blastocysts from SER + MII in both IVF and ICSI cycles yield adequate ongoing pregnancy rates with neo-natal outcomes that do not differ from SER-MII.
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embryological outcomes in cycles with human oocytes containing large tubular Smooth Endoplasmic Reticulum clusters after conventional in vitro fertilization
Gynecological Endocrinology, 2016Co-Authors: Fumiaki Itoi, Yukiko Asano, Masashi Shimizu, Hiroyuki Honnma, Yasutaka MurataAbstract:There have been no studies analyzing the effect of large aggregates of tubular Smooth Endoplasmic Reticulum (aSERT) after conventional in vitro fertilization (cIVF). The aim of this study was to investigate whether aSERT can be identified after cIVF and the association between the embryological outcomes of oocytes in cycles with aSERT. This is a retrospective study examining embryological data from cIVF cycles showing the presence of aSERT in oocytes 5-6 h after cIVF. To evaluate embryo quality, cIVF cycles with at least one aSERT-metaphase II (MII) oocyte observed (cycles with aSERT) were compared to cycles with normal-MII oocytes (control cycles). Among the 4098 MII oocytes observed in 579 cycles, aSERT was detected in 100 MII oocytes in 51 cycles (8.8%). The fertilization rate, the rate of embryo development on day 3 and day 5-6 did not significantly differ between cycles with aSERT and control group. However, aSERT-MII oocytes had lower rates for both blastocysts and good quality blastocysts (p < 0.05). aSERT can be detected in the cytoplasm by removing the cumulus cell 5 h after cIVF. However, aSERT-MII oocytes do not affect other normal-MII oocytes in cycles with aSERT.
Cynthia A Dlugos - One of the best experts on this subject based on the ideXlab platform.
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ethanol related Smooth Endoplasmic Reticulum dilation in purkinje dendrites of aging rats
Alcoholism: Clinical and Experimental Research, 2006Co-Authors: Cynthia A DlugosAbstract:Background: Long-term ethanol consumption in aging rats results in degeneration and regression of the Purkinje neuron (PN) dendritic arbor. One marked ethanol-related change in Purkinje dendrite ultrastructure is dilation of the Smooth Endoplasmic Reticulum (SER) within PN dendritic shafts. The purpose of this study was to determine a time course for ethanol-related dendritic regression in PN dendritic shafts and spines. Methods: One-hundred eighty aging, male Fischer 344 rats were used. Four durations of treatment (5, 10, 20, and 40 weeks) and 3 dietary treatment groups (60 rats/treatment group) were studied. Ethanol-fed rats received a liquid ethanol diet (35% of dietary calories from ethanol). Pair-fed rats received an isocaloric liquid control diet and chow-fed rats received rat chow and water ad libitum. After each duration of treatment, 45 rats (15/treatment) were euthanized and 2 posterior cerebellar lobules/rat were viewed with electron microscopy and photographed. Diameters of SER profiles within PN shafts and spines were measured with image analysis. Results: Ethanol-related SER dilation in dendritic shafts occurred following 40 weeks of treatment. Ethanol-related SER dilation was not detected in PN dendritic spines. Conclusions: These results confirm that ethanol-related dilation of SER profiles in PN dendritic shafts occurs following the same duration of treatment as the dendritic regression previously reported in other studies. Degenerating bodies that may be linked to dendritic regression were also identified in PN dendrites.
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Smooth Endoplasmic Reticulum dilation and degeneration in purkinje neuron dendrites of aging ethanol fed female rats
The Cerebellum, 2006Co-Authors: Cynthia A DlugosAbstract:The effects of chronic ethanol consumption on the extensive Purkinje neuron (PN) dendritic arbor of male rats include dilation of the Smooth Endoplasmic Reticulum (SER) and dendritic regression. The purpose of the present study was to examine the molecular layer of female rats for the presence of ethanol-related SER dilation and evidence of degeneration within the PN dendritic arbor. Twenty-one 12-month-old Fischer 344 female rats (n=7/treatment group) received a liquid ethanol, liquid control, or rat chow diet for a period of 40 weeks. Ethanol-fed rats received 35% of their dietary calories as ethanol. Pair-fed rats received a liquid control diet that was isocaloric to the ethanol diet. Chow-fed rats received standard laboratory rat chow ad libitum. At the end of treatment, tissues from the anterior and posterior lobes of the cerebellar vermis were viewed and photographed with the electron microscope. The diameters of SER profiles were measured and the density of degenerating bodies within the PN dendritic arbor was quantitated. In the posterior lobe, ethanol-related SER dilation was apparent. In the anterior lobe, the density of degenerating bodies within PN dendritic shafts was significantly increased but SER dilation in PN dendritic shafts was absent. These results confirm that SER dilation and dendritic degeneration in PN dendrites may precede and contribute to ethanol-related regression in female rats. In addition, comparison of these results with data obtained in male rats from a previous study suggests that PN dendrites in females may be more sensitive to the effects of ethanol.
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Analyses of Smooth Endoplasmic Reticulum of cerebellar parallel fibers in aging, ethanol-fed rats.
Alcohol, 2005Co-Authors: Cynthia A DlugosAbstract:Abstract The Smooth Endoplasmic Reticulum (SER), a calcium storage organelle, is essential for normal neuronal function. Dilation of the SER is pathologic and a threat to neuronal calcium homeostasis. Dilation of the SER has been reported within the dendrites of cerebellar Purkinje neurons of aging rats after lengthy ethanol treatment. Ethanol-related alterations of parallel fiber SER have not been investigated despite the fact that such dilation may precede and contribute transsynaptically to SER dilation and degeneration in Purkinje neuron dendrites. Male Fischer 344 rats ( n = 120; age = 12 months old) were randomly divided into three dietary groups (40 rats per group) and fed rat chow, the AIN-93M liquid control diet, or the AIN-93M liquid ethanol diet (without water) for 5, 10, 20, or 40 weeks (30 rats per time point). Sections from posterior vermal lobules were viewed with the electron microscope. Maximum and minimum diameters of parallel fiber SER profiles were measured. Ethanol-related dilation of parallel fiber SER was not found after 5, 10, 20, or 40 weeks of treatment. Age-related dilation of parallel fiber SER profiles did occur. These findings support the suggestions that (1) parallel fiber SER, unlike the SER in Purkinje neurons, is insensitive to ethanol and (2) the mechanisms by which ethanol and aging alter cerebellar function and structure are different.
Khaled Pocatecheriet - One of the best experts on this subject based on the ideXlab platform.
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is it time to reconsider how to manage oocytes affected by Smooth Endoplasmic Reticulum aggregates
Human Reproduction, 2019Co-Authors: Lucile Ferreux, Amira Sallem, Ahmed Chargui, Annesophie Gille, Mathilde Bourdon, Chloe Maignien, Pietro Santulli, Jean Philippe Wolf, Catherine Patrat, Khaled PocatecherietAbstract:STUDY QUESTION Did the revised Alpha/ESHRE consensus (Vienna, 2017) bring a real answer on managing oocytes with aggregates of Smooth Endoplasmic Reticulum (SERa)? SUMMARY ANSWER According to the currently available literature, a case by case approach on the time of injecting/inseminating SERa+ oocytes may be not helpful for embryologists making a decision, so we suggest fertilizing both SERa+ and SERa- oocytes and prioritizing embryos derived from SERa- oocytes. WHAT IS KNOWN ALREADY? In 2011, the Istanbul consensus recommended not to inject/inseminate SER+ oocytes due to adverse foetal outcomes reported in literature. At the end of 2017, a panel of experts reconsidered this recommendation and advised a case by case approach. Hence, with a lack of clear recommendations, in-vitro fertilization practitioners still have heterogeneous attitudes when managing SERa+ oocytes. In this context of controversy, an updated review could be helpful in (i) forming a common language for managing cases of SERa+ oocytes and (ii) offering the most ethical practice and best care for patients seeking infertility treatment or fertility preservation. STUDY DESIGN, SIZE, DURATION This review (with a last literature search on 1 June 2018) evaluated the effect of the SER dysmorphism on embryological and neonatal outcomes. PARTICIPANTS/MATERIALS, SETTING, METHODS Studies were considered for inclusion if they were prospective or retrospective cohort or case-control studies. Electronic searches of the Pubmed and Embase databases were done using the keyword combination: Smooth Endoplasmic Reticulum, SER, oocyte and zygote. Abstracts and articles written in English and limited to humans were included. MAIN RESULTS AND THE ROLE OF CHANCE The search returned a total of 726 studies among which 21 met the inclusion criteria. The literature does not unanimously support a negative association between SERa and embryogenesis, implantation or assisted reproductive therapy outcomes. The reviewed studies reported 112 neonatal outcomes after transfers where at least one embryo originated from oocyte affected by SERa. They included 101 healthy babies, three live births with malformations, three neonatal deaths, one stillbirth and four medical interruptions of pregnancy. After transfer of embryos exclusively derived from SERa+ oocytes, a total of 48 healthy newborns were reported along with four babies with perinatal complications (including one ventricular septal defect), one stillbirth, one neonatal death and one pregnancy termination for multiple malformations. LIMITATIONS, REASONS FOR CAUTION As with any review, this review was limited by the quality of the included studies especially in terms of possible methodological limitations, the limited sample size and the retrospective aspect of the studies. Among the 21 selected studies, seven were abstracts and two were case reports. Of the remaining 14 studies, only three were prospective. The tools used in identifying SERa+ oocytes may have varied from one study to another and a consequent misclassification cannot be excluded. Considering the poor resolution of light microscopy in detecting SER aggregates, we are not sure that apparently SERa- oocytes do not really exhibit such a dysmorphism if they were analysed under electronic microscopy or a time lapse system. WIDER IMPLICATIONS OF THE FINDINGS In the light of the existing data and the lack of a real link between fertilizing SERa+ oocytes and the occurrence of embryo aneuploidy/malformations, we think that discarding SERa+ oocytes may be not the most ethical approach even in patients with large cohorts on the day of oocyte retrieval. Avoiding the wastage of oocytes and embryos with respect to medical ethics remains a constant concern in daily IVF practice. Thus, we recommend that all mature oocytes could be fertilized and embryos originating from SERa- oocytes would be preferably transferred, even if they come from a cohort with SERa+ oocytes. The remaining embryos derived from SERa+ oocytes could be considered with a lower priority for transfer after obtaining consent from the couple if a strict follow-up of the pregnancy and the baby is performed. STUDY FUNDING/COMPETING INTEREST(S) We have no conflict of interest to declare and no funding was received. REGISTRATION NUMBER N/A.
