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Mark E Haacke - One of the best experts on this subject based on the ideXlab platform.

  • in ovo monitoring of Smooth Muscle Fiber development in the chick embryo diffusion tensor imaging with histologic correlation
    PLOS ONE, 2012
    Co-Authors: Zachary Delproposto, Zien Zhou, Huicong Shen, Stephanie Y Xuan, Mark E Haacke
    Abstract:

    Background Magnetic resonance imaging is a noninvasive method of evaluating embryonic development. Magnetic resonance diffusion tensor imaging, which is based on the measuring the directional diffusivity of water molecules, is an established method of evaluating tissue structure. Prolonged imaging times have precluded the use of embryonic diffusion tensor imaging due to motion artifact. Using temperature-based motion suppression, we aimed to investigate whether diffusion tensor imaging can be used to monitor embryonic Smooth Muscle development in ovo, and to determine the correlation between histologically-derived Muscle Fiber fraction, day of incubation and diffusion tensor imaging fractional anisotropy values and length of tracked Fibers. Methodology/Principal Findings From a set of 82 normally developing fertile chicken eggs, 5 eggs were randomly chosen each day from incubation days 5 to 18 and cooled using a dual-cooling technique prior to and during magnetic resonance imaging at 3.0 Tesla. Smooth Muscle Fibers of the gizzard were tracked using region of interests placed over the gizzard. Following imaging, the egg was cracked and the embryo was fixated and sectioned, and a micrograph most closely corresponding to the acquired magnetic resonance image was made. Smooth Muscle Fiber fraction was determined using an automated computer algorithm. Conclusions/Significance We show that diffusion tensor images of Smooth Muscle within the embryonic gizzard can be acquired in ovo from incubation day 11 through hatching. Length of tracked Fibers and day of incubation were found to have statistical significance (p<0.05) by multiple linear regression correlation with histologic specimens of sacrificed embryos from day 11 of incubation through hatching. The morphologic pattern of development in our histologic specimens corresponds to the development of embryonic gizzard as reported in the literature. These results suggest that diffusion tensor imaging can provide a noninvasive method of evaluating in ovo development of Smooth Muscle tissue.

Muñoz De Toro, Monica Milagros - One of the best experts on this subject based on the ideXlab platform.

  • The external genitalia in juvenile Caiman latirostris differ in hormone sex determinate-female from temperature sex determinate-female
    Academic Press Inc Elsevier Science, 2019
    Co-Authors: Tavalieri, Yamil Ezequiel, Galoppo, Germán Hugo, Canesini Guillermina, Truter J.c., Ramos, Jorge Guillermo, Luque, Enrique Hugo, Muñoz De Toro, Monica Milagros
    Abstract:

    The broad-snouted caiman (Caiman latirostris) is a crocodilian species that inhabits South American wetlands. As in all other crocodilians, the egg incubation temperature during a critical thermo-sensitive window (TSW) determines the sex of the hatchlings, a phenomenon known as temperature-dependent sex determination (TSD). In C. latirostris, we have shown that administration of 17-β-estradiol (E 2 ) during the TSW overrides the effect of the male-producing temperature, producing phenotypic females (E 2 SD-females). Moreover, the administration of E 2 during TSW has been proposed as an alternative way to improve the recovery of endangered reptile species, by skewing the population sex ratio to one that favors females. However, the ovaries of E 2 SD-female caimans differ from those of TSD-females. In crocodilians, the external genitalia (i.e. clitero-penis structure or phallus) are sexually dimorphic and hormone-sensitive. Despite some morphological descriptions aimed to facilitate sexing, we found no available data on the C. latirostris phallus histoarchitecture or hormone dependence. Thus, the aims of this study were: (1) to establish the temporal growth pattern of the phallus in male and female caimans; (2) to evaluate histo-morphological features and the expression of estrogen receptor alpha (ERα) and androgen receptor (AR) in the phallus of male and female pre-pubertal juvenile caimans; and (3) to determine whether the phallus of TSD-females differs from the phallus of E 2 SD-females. Our results demonstrated sexually dimorphic differences in the size and growth dynamics of the caiman external genitalia, similarities in the shape and spatial distribution of general histo-morphological compartments, and sexually dimorphic differences in innervation, Smooth Muscle Fiber distribution, collagen organization, and ERα and AR expressions. The external genitalia of E 2 SD-females differed from that of TSD-females in many histological features and in the expression of ERα and AR, resembling patterns described in males. Our results alert on the effects of estrogen agonist exposure during TSW and suggest that caution must be taken regarding the use of E 2 SD as a procedure for wildlife population management

  • The external genitalia in juvenile Caiman latirostris differ in hormone sex determinate-female from temperature sex determinate-female
    'Elsevier BV', 2019
    Co-Authors: Tavalieri, Yamil Ezequiel, Galoppo, Germán Hugo, Canesini Guillermina, Truter J.c., Ramos, Jorge Guillermo, Luque, Enrique Hugo, Muñoz De Toro, Monica Milagros
    Abstract:

    The broad-snouted caiman (Caiman latirostris) is a crocodilian species that inhabits South American wetlands. As in all other crocodilians, the egg incubation temperature during a critical thermo-sensitive window (TSW) determines the sex of the hatchlings, a phenomenon known as temperature-dependent sex determination (TSD). In C. latirostris, we have shown that administration of 17-β-estradiol (E 2 ) during the TSW overrides the effect of the male-producing temperature, producing phenotypic females (E 2 SD-females). Moreover, the administration of E 2 during TSW has been proposed as an alternative way to improve the recovery of endangered reptile species, by skewing the population sex ratio to one that favors females. However, the ovaries of E 2 SD-female caimans differ from those of TSD-females. In crocodilians, the external genitalia (i.e. clitero-penis structure or phallus) are sexually dimorphic and hormone-sensitive. Despite some morphological descriptions aimed to facilitate sexing, we found no available data on the C. latirostris phallus histoarchitecture or hormone dependence. Thus, the aims of this study were: (1) to establish the temporal growth pattern of the phallus in male and female caimans; (2) to evaluate histo-morphological features and the expression of estrogen receptor alpha (ERα) and androgen receptor (AR) in the phallus of male and female pre-pubertal juvenile caimans; and (3) to determine whether the phallus of TSD-females differs from the phallus of E 2 SD-females. Our results demonstrated sexually dimorphic differences in the size and growth dynamics of the caiman external genitalia, similarities in the shape and spatial distribution of general histo-morphological compartments, and sexually dimorphic differences in innervation, Smooth Muscle Fiber distribution, collagen organization, and ERα and AR expressions. The external genitalia of E 2 SD-females differed from that of TSD-females in many histological features and in the expression of ERα and AR, resembling patterns described in males. Our results alert on the effects of estrogen agonist exposure during TSW and suggest that caution must be taken regarding the use of E 2 SD as a procedure for wildlife population management.Fil: Tavalieri, Yamil Ezequiel. Universidad Nacional del Litoral; ArgentinaFil: Galoppo, Germán Hugo. Universidad Nacional del Litoral; ArgentinaFil: Canesini, Guillermina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; ArgentinaFil: Truter, J.C.. STELLENBOSCH UNIVERSITY (SUN);Fil: Ramos, Jorge Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; ArgentinaFil: Luque, Enrique Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; ArgentinaFil: Muñoz de Toro, Monica Milagros. Universidad Nacional del Litoral; Argentin

Zachary Delproposto - One of the best experts on this subject based on the ideXlab platform.

  • in ovo monitoring of Smooth Muscle Fiber development in the chick embryo diffusion tensor imaging with histologic correlation
    PLOS ONE, 2012
    Co-Authors: Zachary Delproposto, Zien Zhou, Huicong Shen, Stephanie Y Xuan, Mark E Haacke
    Abstract:

    Background Magnetic resonance imaging is a noninvasive method of evaluating embryonic development. Magnetic resonance diffusion tensor imaging, which is based on the measuring the directional diffusivity of water molecules, is an established method of evaluating tissue structure. Prolonged imaging times have precluded the use of embryonic diffusion tensor imaging due to motion artifact. Using temperature-based motion suppression, we aimed to investigate whether diffusion tensor imaging can be used to monitor embryonic Smooth Muscle development in ovo, and to determine the correlation between histologically-derived Muscle Fiber fraction, day of incubation and diffusion tensor imaging fractional anisotropy values and length of tracked Fibers. Methodology/Principal Findings From a set of 82 normally developing fertile chicken eggs, 5 eggs were randomly chosen each day from incubation days 5 to 18 and cooled using a dual-cooling technique prior to and during magnetic resonance imaging at 3.0 Tesla. Smooth Muscle Fibers of the gizzard were tracked using region of interests placed over the gizzard. Following imaging, the egg was cracked and the embryo was fixated and sectioned, and a micrograph most closely corresponding to the acquired magnetic resonance image was made. Smooth Muscle Fiber fraction was determined using an automated computer algorithm. Conclusions/Significance We show that diffusion tensor images of Smooth Muscle within the embryonic gizzard can be acquired in ovo from incubation day 11 through hatching. Length of tracked Fibers and day of incubation were found to have statistical significance (p<0.05) by multiple linear regression correlation with histologic specimens of sacrificed embryos from day 11 of incubation through hatching. The morphologic pattern of development in our histologic specimens corresponds to the development of embryonic gizzard as reported in the literature. These results suggest that diffusion tensor imaging can provide a noninvasive method of evaluating in ovo development of Smooth Muscle tissue.

Tavalieri, Yamil Ezequiel - One of the best experts on this subject based on the ideXlab platform.

  • The external genitalia in juvenile Caiman latirostris differ in hormone sex determinate-female from temperature sex determinate-female
    Academic Press Inc Elsevier Science, 2019
    Co-Authors: Tavalieri, Yamil Ezequiel, Galoppo, Germán Hugo, Canesini Guillermina, Truter J.c., Ramos, Jorge Guillermo, Luque, Enrique Hugo, Muñoz De Toro, Monica Milagros
    Abstract:

    The broad-snouted caiman (Caiman latirostris) is a crocodilian species that inhabits South American wetlands. As in all other crocodilians, the egg incubation temperature during a critical thermo-sensitive window (TSW) determines the sex of the hatchlings, a phenomenon known as temperature-dependent sex determination (TSD). In C. latirostris, we have shown that administration of 17-β-estradiol (E 2 ) during the TSW overrides the effect of the male-producing temperature, producing phenotypic females (E 2 SD-females). Moreover, the administration of E 2 during TSW has been proposed as an alternative way to improve the recovery of endangered reptile species, by skewing the population sex ratio to one that favors females. However, the ovaries of E 2 SD-female caimans differ from those of TSD-females. In crocodilians, the external genitalia (i.e. clitero-penis structure or phallus) are sexually dimorphic and hormone-sensitive. Despite some morphological descriptions aimed to facilitate sexing, we found no available data on the C. latirostris phallus histoarchitecture or hormone dependence. Thus, the aims of this study were: (1) to establish the temporal growth pattern of the phallus in male and female caimans; (2) to evaluate histo-morphological features and the expression of estrogen receptor alpha (ERα) and androgen receptor (AR) in the phallus of male and female pre-pubertal juvenile caimans; and (3) to determine whether the phallus of TSD-females differs from the phallus of E 2 SD-females. Our results demonstrated sexually dimorphic differences in the size and growth dynamics of the caiman external genitalia, similarities in the shape and spatial distribution of general histo-morphological compartments, and sexually dimorphic differences in innervation, Smooth Muscle Fiber distribution, collagen organization, and ERα and AR expressions. The external genitalia of E 2 SD-females differed from that of TSD-females in many histological features and in the expression of ERα and AR, resembling patterns described in males. Our results alert on the effects of estrogen agonist exposure during TSW and suggest that caution must be taken regarding the use of E 2 SD as a procedure for wildlife population management

  • The external genitalia in juvenile Caiman latirostris differ in hormone sex determinate-female from temperature sex determinate-female
    'Elsevier BV', 2019
    Co-Authors: Tavalieri, Yamil Ezequiel, Galoppo, Germán Hugo, Canesini Guillermina, Truter J.c., Ramos, Jorge Guillermo, Luque, Enrique Hugo, Muñoz De Toro, Monica Milagros
    Abstract:

    The broad-snouted caiman (Caiman latirostris) is a crocodilian species that inhabits South American wetlands. As in all other crocodilians, the egg incubation temperature during a critical thermo-sensitive window (TSW) determines the sex of the hatchlings, a phenomenon known as temperature-dependent sex determination (TSD). In C. latirostris, we have shown that administration of 17-β-estradiol (E 2 ) during the TSW overrides the effect of the male-producing temperature, producing phenotypic females (E 2 SD-females). Moreover, the administration of E 2 during TSW has been proposed as an alternative way to improve the recovery of endangered reptile species, by skewing the population sex ratio to one that favors females. However, the ovaries of E 2 SD-female caimans differ from those of TSD-females. In crocodilians, the external genitalia (i.e. clitero-penis structure or phallus) are sexually dimorphic and hormone-sensitive. Despite some morphological descriptions aimed to facilitate sexing, we found no available data on the C. latirostris phallus histoarchitecture or hormone dependence. Thus, the aims of this study were: (1) to establish the temporal growth pattern of the phallus in male and female caimans; (2) to evaluate histo-morphological features and the expression of estrogen receptor alpha (ERα) and androgen receptor (AR) in the phallus of male and female pre-pubertal juvenile caimans; and (3) to determine whether the phallus of TSD-females differs from the phallus of E 2 SD-females. Our results demonstrated sexually dimorphic differences in the size and growth dynamics of the caiman external genitalia, similarities in the shape and spatial distribution of general histo-morphological compartments, and sexually dimorphic differences in innervation, Smooth Muscle Fiber distribution, collagen organization, and ERα and AR expressions. The external genitalia of E 2 SD-females differed from that of TSD-females in many histological features and in the expression of ERα and AR, resembling patterns described in males. Our results alert on the effects of estrogen agonist exposure during TSW and suggest that caution must be taken regarding the use of E 2 SD as a procedure for wildlife population management.Fil: Tavalieri, Yamil Ezequiel. Universidad Nacional del Litoral; ArgentinaFil: Galoppo, Germán Hugo. Universidad Nacional del Litoral; ArgentinaFil: Canesini, Guillermina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; ArgentinaFil: Truter, J.C.. STELLENBOSCH UNIVERSITY (SUN);Fil: Ramos, Jorge Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; ArgentinaFil: Luque, Enrique Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; ArgentinaFil: Muñoz de Toro, Monica Milagros. Universidad Nacional del Litoral; Argentin

Stephanie Y Xuan - One of the best experts on this subject based on the ideXlab platform.

  • in ovo monitoring of Smooth Muscle Fiber development in the chick embryo diffusion tensor imaging with histologic correlation
    PLOS ONE, 2012
    Co-Authors: Zachary Delproposto, Zien Zhou, Huicong Shen, Stephanie Y Xuan, Mark E Haacke
    Abstract:

    Background Magnetic resonance imaging is a noninvasive method of evaluating embryonic development. Magnetic resonance diffusion tensor imaging, which is based on the measuring the directional diffusivity of water molecules, is an established method of evaluating tissue structure. Prolonged imaging times have precluded the use of embryonic diffusion tensor imaging due to motion artifact. Using temperature-based motion suppression, we aimed to investigate whether diffusion tensor imaging can be used to monitor embryonic Smooth Muscle development in ovo, and to determine the correlation between histologically-derived Muscle Fiber fraction, day of incubation and diffusion tensor imaging fractional anisotropy values and length of tracked Fibers. Methodology/Principal Findings From a set of 82 normally developing fertile chicken eggs, 5 eggs were randomly chosen each day from incubation days 5 to 18 and cooled using a dual-cooling technique prior to and during magnetic resonance imaging at 3.0 Tesla. Smooth Muscle Fibers of the gizzard were tracked using region of interests placed over the gizzard. Following imaging, the egg was cracked and the embryo was fixated and sectioned, and a micrograph most closely corresponding to the acquired magnetic resonance image was made. Smooth Muscle Fiber fraction was determined using an automated computer algorithm. Conclusions/Significance We show that diffusion tensor images of Smooth Muscle within the embryonic gizzard can be acquired in ovo from incubation day 11 through hatching. Length of tracked Fibers and day of incubation were found to have statistical significance (p<0.05) by multiple linear regression correlation with histologic specimens of sacrificed embryos from day 11 of incubation through hatching. The morphologic pattern of development in our histologic specimens corresponds to the development of embryonic gizzard as reported in the literature. These results suggest that diffusion tensor imaging can provide a noninvasive method of evaluating in ovo development of Smooth Muscle tissue.