The Experts below are selected from a list of 1656 Experts worldwide ranked by ideXlab platform
Li Lin - One of the best experts on this subject based on the ideXlab platform.
-
induction of apoptosis in ssn 1cells by Snakehead Fish vesiculovirus shvv via matrix protein dependent intrinsic pathway
Fish & Shellfish Immunology, 2021Co-Authors: Nan Chen, Abeer M Hegazy, Hanzuo Lin, Sarath V Babu, Youcheng Yang, Zhendong Qin, Fei Shi, Li LinAbstract:Abstract An increasing important area in immunology is the process cell death mechanism, enabling the immune system triggered thru extrinsic or intrinsic signals to effectively remove unwanted or virus infected cells called apoptosis. A recently isolated infectious Snakehead Fish vesiculovirus (SHVV), comprising negative strand RNA and encoded viral matrix (M) proteins, is responsible for causing cytopathic effects in infected Fish cells. However, the mechanism by which viral M protein mediates apoptosis has not been elucidated. Therefore, in the present experiments, it was investigated the regulatory potential of apoptosis signals during SHVV infection. By employing the model of SHVV infection in SSN-1 cells, the accelerated apoptosis pathway involves an intrinsic pathway requiring the activation of caspase-9 but not caspase-3 or -8. In the groups of infection (SHVV) or treatment (hydrogen peroxide) were induced apoptotic morphological changes and indicated the activation of the main caspases, i.e.; executioner caspase-3, initiators caspase-8 and caspase-9 using colorimetric assays. Turning to the role of viral M protein when it was overexpressed in SSN-1 cells, it was indicated that the viral M gene alone has the ability to induce apoptosis. To elucidate the mechanism of apoptosis in SSN-1 cells, the activation inhibitors of main caspases were used showing that inhibiting of caspase-3 or caspase-8 activation did not seize induction of apoptosis in virus-infected SSN-1 cells. However, the inhibiting of caspase-9 activation reduced significantly the apoptosis initiation process and sharply the expression of viral M gene, suggesting that SHVV plays a major role in the early induction of apoptosis by caspase-9. Interestingly, there were also differences in the mitochondrial membrane potential after the apoptotic induction of caspases, which confirm that caspase-9 is primarily responsible for the cleavage of caspases during apoptosis. Taken together, these findings can therefore be assumed that viral M protein induces apoptosis via the intrinsic apoptotic pathway in SHVV infecting SSN-1 cells.
-
transcriptomic profiles of striped Snakehead Fish cells ssn 1 infected with red spotted grouper nervous necrosis virus rgnnv with an emphasis on apoptosis pathway
Fish & Shellfish Immunology, 2017Co-Authors: Wenjie Chen, Xiaodan Liu, Li Lin, Jiangfeng Lan, Shuangshuang Feng, Muhammad Asim, Yongcan Zhou, Sanjie JiangAbstract:Nervous necrosis virus (NNV), the causative agent of viral nervous necrosis (VNN) disease, has caused mass mortality of cultured marine and freshwater Fish worldwide, resulting in enormous economic losses in the aquaculture industry. However, the molecular mechanisms underlying the pathogenicity of NNV are still poorly understood. In this study, the transcriptomic profiles of striped Snakehead Fish (Channa striatus) cells (SSN-1) infected with red-spotted grouper NNV (RGNNV) were investigated using deep RNA sequencing technique. From 254,955,234 raw reads, a total of 253,338,544 clean reads were obtained and they were assembled into 93,372 unigenes. Differentially expressed genes (DEGs) were identified from RGNNV-infected or mock-infected SSN-1 cells, including 1184 up-regulated and 1456 down-regulated genes at 3 h (h) post of infection (poi), and 1138 up-regulated and 2073 down-regulated genes at 24 h poi, respectively. These DEGs were involved in many pathways related to viral pathogenesis, including retinoic acid-inducible gene I (RIG-I) like receptors pathway, apoptosis pathway, oxidative phosphorylation, PI3K-Akt signaling pathway, and MAPK signaling pathway. Subsequent analysis focusing on the apoptosis pathway showed that the expression of Endonuclease G (EndoG) was up-regulated upon RGNNV infection at both 3 and 24 h poi. Therefore, EndoG gene was cloned and its function was further characterized. The results showed that over-expression of EndoG could also induce cellular apoptosis in SSN-1 cells, indicating that RGNNV infection might induce apoptosis of SSN-1 cells via EndoG-associated mitochondrial pathway. These results will shed a new light on the pathogenesis of NNV.
-
glutamine is required for Snakehead Fish vesiculovirus propagation via replenishing the tricarboxylic acid cycle
Journal of General Virology, 2016Co-Authors: Lindan Sun, Xiaodan Liu, Li Lin, Wenjie Chen, Lijuan Zhao, Jiangfeng Lan, Shuangshuang Feng, Chi ZhangAbstract:Snakehead Fish vesiculovirus (SHVV), a member of the family Rhabdoviridae, has caused mass mortality in Snakehead Fish culture in China. Previous transcriptomic sequencing of SHVV-infected and non-infected striped Snakehead Fish cells (SSN-1) showed that glutaminase (GLS), the critical enzyme of glutamine metabolism, was upregulated upon SHVV infection. It therefore drew our attention to investigating the role of glutamine in SHVV propagation. Glutamine deprivation significantly reduced the expression of the mRNAs and proteins of SHVV, and the production of virus particles, indicating that glutamine was required for SHVV propagation. Glutamine can be converted to glutamate by GLS, and then be converted to α-ketoglutarate, to join in the tricarboxylic acid (TCA) cycle. Addition of the TCA cycle intermediate α-ketoglutarate, oxaloacetic acid or pyruvate significantly restored SHVV propagation, indicating that the requirement of glutamine for SHVV propagation was due to its replenishment of the TCA cycle. Inhibiting the activity of GLS in SSN-1 cells by an inhibitor, bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl)ethyl sulfide, decreased SHVV propagation, while overexpression of GLS increased SHVV propagation. Taken together, our data have revealed the relationship between glutamine metabolism and SHVV propagation.
-
autophagy induced by Snakehead Fish vesiculovirus inhibited its replication in ssn 1 cell line
Fish & Shellfish Immunology, 2016Co-Authors: Yao Wang, Xiaodan Liu, Lijuan Zhao, Nan Chen, Abeer M Hegazy, Xueqin Liu, Qiwei Qin, Jiangfeng Lan, Li LinAbstract:Autophagy plays an important role in host protection against pathogen infection through activating innate and adaptive immunity. In the present study, we observed that the infection of Snakehead Fish vesiculovirus (SHVV) could induce apparent autophagy in striped Snakehead Fish cell line (SSN-1), including clear double-membrane vesicles, fluorescent punctate pattern of microtubule-associated protein 1 light chain 3B (SSN-LC3B) and the conversion of SSN-LC3B-Ⅰ to SSN-LC3B-Ⅱ. Furthermore, we verified that autophagy inhibited the replication of SHVV by assessing mRNA and protein level of nucleoprotein as well as virus titer in the supernatant. These results will shed a new light on the prevention of the infection of SHVV.
-
Article Abortive Infection of Snakehead Fish Vesiculovirus in ZF4 Cells Was Associated with the RLRs Pathway Activation by Viral Replicative Intermediates
2015Co-Authors: Wenwen Wang, Abeer M Hegazy, Muhammad Asim, Yang Zhou, Li LinAbstract:Abstract: Snakehead Fish vesiculovirus (SHVV) is a negative strand RNA virus which can cause great economic losses in Fish culture. To facilitate the study of SHVV-host interactions, the susceptibility of zebraFish embryonic fibroblast cell line (ZF4) to the SHVV was investigated in this report. The results showed that high amount of viral mRNAs and cRNAs were detected at the 3 h post-infection. However, the expressions of the viral mRNAs and cRNA were decreased dramatically after 6 h post-infection. In addition, the expressions of interferon (IFN) and interferon-induced GTP-binding protein Mx were all up regulated significantly at the late stage of the infection. Meanwhile, the expressions of Retinoic acid-inducible gene I (RIG-I) and Melanoma OPEN ACCESS Int. J. Mol. Sci. 2015, 16 6236 differentiation-associated gene 5 (MDA5) were also all up-regulated significantly durin
Xiaodan Liu - One of the best experts on this subject based on the ideXlab platform.
-
susceptibility and immune responses of hybrid Snakehead channa maculata channa argus following infection with Snakehead Fish vesiculovirus
Aquaculture, 2021Co-Authors: Pan Cao, Wei Sun, Yanbing Zhang, Zicheng Zhou, Xiaojun Zhang, Xiaodan LiuAbstract:Abstract Snakehead Fish vesiculovirus (SHVV) was initially isolated from diseased hybrid Snakehead, and could cause great economic losses in Snakehead culture due to its high morbidity and mortality. In this study, the susceptibility, histopathological analysis, and host immune response of hybrid Snakehead infected with SHVV were investigated. SHVV was highly pathogenic to hybrid Snakehead, and the infected Fish exhibited typical signs of acute haemorrhages, and enlarged visceral organs. Histopathological analysis showed that the major tissues of diseased Fish exhibited obvious inflammatory responses to SHVV infection. Additionally, humoral immune factors such as superoxide dismutase (SOD), catalase (CAT), and acid phosphatase (ACP) were assayed as serum indicators to evaluate the immune responses induced in infected hybrid Snakehead. Quantitative real-time PCR (qRT-PCR) was also performed to evaluate the expression patterns of immune-related genes (HSP70, IL-8, MHCIα, MHCIIα, SOD1, ZAP70, PKCθ, LCK, and HLA-A) in tissues of liver, spleen, head kidney and intestine. The results showed significantly enhanced immune activities in hybrid Snakehead after SHVV infection. Altogether, these data provided insights into the pathogenic mechanism of SHVV and host immune response, facilitating the future prevention and treatment of hybrid Snakehead infected with SHVV.
-
transcriptomic profiles of striped Snakehead Fish cells ssn 1 infected with red spotted grouper nervous necrosis virus rgnnv with an emphasis on apoptosis pathway
Fish & Shellfish Immunology, 2017Co-Authors: Wenjie Chen, Xiaodan Liu, Li Lin, Jiangfeng Lan, Shuangshuang Feng, Muhammad Asim, Yongcan Zhou, Sanjie JiangAbstract:Nervous necrosis virus (NNV), the causative agent of viral nervous necrosis (VNN) disease, has caused mass mortality of cultured marine and freshwater Fish worldwide, resulting in enormous economic losses in the aquaculture industry. However, the molecular mechanisms underlying the pathogenicity of NNV are still poorly understood. In this study, the transcriptomic profiles of striped Snakehead Fish (Channa striatus) cells (SSN-1) infected with red-spotted grouper NNV (RGNNV) were investigated using deep RNA sequencing technique. From 254,955,234 raw reads, a total of 253,338,544 clean reads were obtained and they were assembled into 93,372 unigenes. Differentially expressed genes (DEGs) were identified from RGNNV-infected or mock-infected SSN-1 cells, including 1184 up-regulated and 1456 down-regulated genes at 3 h (h) post of infection (poi), and 1138 up-regulated and 2073 down-regulated genes at 24 h poi, respectively. These DEGs were involved in many pathways related to viral pathogenesis, including retinoic acid-inducible gene I (RIG-I) like receptors pathway, apoptosis pathway, oxidative phosphorylation, PI3K-Akt signaling pathway, and MAPK signaling pathway. Subsequent analysis focusing on the apoptosis pathway showed that the expression of Endonuclease G (EndoG) was up-regulated upon RGNNV infection at both 3 and 24 h poi. Therefore, EndoG gene was cloned and its function was further characterized. The results showed that over-expression of EndoG could also induce cellular apoptosis in SSN-1 cells, indicating that RGNNV infection might induce apoptosis of SSN-1 cells via EndoG-associated mitochondrial pathway. These results will shed a new light on the pathogenesis of NNV.
-
glutamine is required for Snakehead Fish vesiculovirus propagation via replenishing the tricarboxylic acid cycle
Journal of General Virology, 2016Co-Authors: Lindan Sun, Xiaodan Liu, Li Lin, Wenjie Chen, Lijuan Zhao, Jiangfeng Lan, Shuangshuang Feng, Chi ZhangAbstract:Snakehead Fish vesiculovirus (SHVV), a member of the family Rhabdoviridae, has caused mass mortality in Snakehead Fish culture in China. Previous transcriptomic sequencing of SHVV-infected and non-infected striped Snakehead Fish cells (SSN-1) showed that glutaminase (GLS), the critical enzyme of glutamine metabolism, was upregulated upon SHVV infection. It therefore drew our attention to investigating the role of glutamine in SHVV propagation. Glutamine deprivation significantly reduced the expression of the mRNAs and proteins of SHVV, and the production of virus particles, indicating that glutamine was required for SHVV propagation. Glutamine can be converted to glutamate by GLS, and then be converted to α-ketoglutarate, to join in the tricarboxylic acid (TCA) cycle. Addition of the TCA cycle intermediate α-ketoglutarate, oxaloacetic acid or pyruvate significantly restored SHVV propagation, indicating that the requirement of glutamine for SHVV propagation was due to its replenishment of the TCA cycle. Inhibiting the activity of GLS in SSN-1 cells by an inhibitor, bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl)ethyl sulfide, decreased SHVV propagation, while overexpression of GLS increased SHVV propagation. Taken together, our data have revealed the relationship between glutamine metabolism and SHVV propagation.
-
autophagy induced by Snakehead Fish vesiculovirus inhibited its replication in ssn 1 cell line
Fish & Shellfish Immunology, 2016Co-Authors: Yao Wang, Xiaodan Liu, Lijuan Zhao, Nan Chen, Abeer M Hegazy, Xueqin Liu, Qiwei Qin, Jiangfeng Lan, Li LinAbstract:Autophagy plays an important role in host protection against pathogen infection through activating innate and adaptive immunity. In the present study, we observed that the infection of Snakehead Fish vesiculovirus (SHVV) could induce apparent autophagy in striped Snakehead Fish cell line (SSN-1), including clear double-membrane vesicles, fluorescent punctate pattern of microtubule-associated protein 1 light chain 3B (SSN-LC3B) and the conversion of SSN-LC3B-Ⅰ to SSN-LC3B-Ⅱ. Furthermore, we verified that autophagy inhibited the replication of SHVV by assessing mRNA and protein level of nucleoprotein as well as virus titer in the supernatant. These results will shed a new light on the prevention of the infection of SHVV.
-
identification and characterization of micrornas in Snakehead Fish cell line upon Snakehead Fish vesiculovirus infection
International Journal of Molecular Sciences, 2016Co-Authors: Xiaodan Liu, Lijuan Zhao, Nan Chen, Abeer M Hegazy, Xueqin Liu, Junfa Yuan, Farman Ullah Dawar, Muhammad Nasir Khan Khattak, Vikram N VakhariaAbstract:MicroRNAs (miRNAs) play important roles in mediating multiple biological processes in eukaryotes and are being increasingly studied to evaluate their roles associated with cellular changes following viral infection. Snakehead Fish Vesiculovirus (SHVV) has caused mass mortality in Snakehead Fish during the past few years. To identify specific miRNAs involved in SHVV infection, we performed microRNA deep sequencing on a Snakehead Fish cell line (SSN-1) with or without SHVV infection. A total of 205 known miRNAs were identified when they were aligned with the known zebraFish miRNAs, and nine novel miRNAs were identified using MiRDeep2 software. Eighteen and 143 of the 205 known miRNAs were differentially expressed at three and 24 h post-infection (poi), respectively. From the differentially-expressed miRNAs, five were randomly selected to validate their expression profiles using quantitative reverse transcription polymerase chain reaction (qRT-PCR), and their expression profiles were consistent with the microRNA sequencing results. In addition, the target gene prediction of the SHVV genome was performed for the differentially-expressed host miRNAs, and a total of 10 and 58 differentially-expressed miRNAs were predicted to bind to the SHVV genome at three and 24 h poi, respectively. The effects of three selected miRNAs (miR-130-5p, miR-214 and miR-216b) on SHVV multiplication were evaluated using their mimics and inhibitors via qRT-PCR and Western blotting. The results showed that all three miRNAs were able to inhibit the multiplication of SHVV; whereas the mechanisms underlying the SHVV multiplication inhibited by the specific miRNAs need to be further characterized in the future.
Kasi Marimuthu - One of the best experts on this subject based on the ideXlab platform.
-
effect of different cooking methods on proximate and mineral composition of striped Snakehead Fish channa striatus bloch
Journal of Food Science and Technology-mysore, 2012Co-Authors: Kasi Marimuthu, R Xavier, M Thilaga, Sekar Kathiresan, R H M H MasAbstract:The effects of different cooking methods (boiling, baking, frying and grilling) on proximate and mineral composition of Snakehead Fish were investigated. The mean content of moisture, protein, fat and ash of raw Fish was found to be 77.2 ± 2.39, 13.9 ± 2.89, 5.9 ± 0.45 and 0.77 ± 0.12% respectively. The changes in the amount of protein and fat were found to be significantly higher in frying and grilling Fish. The ash content increased significantly whereas that of the minerals (Na, K, Ca, Mg, Fe, Zn and Mn) was not affected in all cooking methods. Increased in Cu contents and decreased in P contents were observed in all cooking methods except grilling. In the present study, the grilling method of cooking is found to be the best for healthy eating.
-
antimicrobial activity of different tissues of Snakehead Fish channa striatus bloch
Asian Pacific Journal of Tropical Disease, 2012Co-Authors: Pravin N Kumar, R Xavier, Kasi Marimuthu, Sekar Kathiresan, Vengkades R Rao, Chinjusha Suresh, Subramaniam SreeramananAbstract:Abstract Objective The aim of this study was to identify the presence of antimicrobial activity in different organs/tissues (gills, blood, skin, liver, intestine, kidney, tissue and ovary) extract of Snakehead Fish Channa striatus. Methods A total of 48 fractions from the organs and tissue extracts were obtained by solid-phase extraction and the fractions were assayed for antimicrobial activity. The screening of antimicrobial activity for all the fractions were tested against 8 human pathogens including Gram positive (Methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus aureus, Bacillus cereus ) and Gram negative bacteria ( Salmonella enteritidis, Shigella flexneri, Acinetobacter baumanni, Escherichia coli, Klebsiella pneumoniae ) using the British Society for Antimicrobial Chemotherapy (BSAC) standardized disc susceptibility test method. The activity was measured in terms of zone of inhibition in mm. Results The results indicated that, among the 8 organs/tissues tested only blood and gills extract fractions (40 and 60 % ACN fraction) showed inhibition against Escherichia coli and 60 % ACN fraction of gill extract showed inhibition against Salmonella enteritidis . Protein profile analysis by SDS-PAGE showed that antimicrobial activity of the partially purified blood and gill tissue extracts might be due to low molecular weight peptides. Conclusions The present study showed that, gill and blood extracts of Channa striatus can be a potential source of an antimicrobial protein for specific human pathogens.
-
screening of antibacterial activity of mucus extract of Snakehead Fish channa striatus bloch
European Review for Medical and Pharmacological Sciences, 2010Co-Authors: Ong Yeong Wei, R Xavier, Kasi MarimuthuAbstract:Aim: The objective of this study is to gain a better understanding of the antimicrobial properties of the mucus extract of Snakehead Fish, Channa striatus against se- lected human and Fish pathogenic microbes. Materials and Methods: The Fish mucus samples were extracted with crude, acidic and aqueous solvents to identify potential antimi- crobial agents including aqueous and acid soluble compounds. The study also deter- mined the protein content of the three differ- ent mucus extracts. The highest protein con- tent (0.589 mg/ml) was noticed in the crude ex- tract followed by aqueous mucus extract (0.291 mg/ml) and acidic extract (0.267 mg/ml). Preliminary screening for antimicrobial activi- ty of all three mucus extracts were tested against 5 human pathogens (Bacillus subtilis, Klebsiella pneumoniae, Salmonella enteritidis, Proteus vulgaris and Pseudomonas aeruginosa) and Fish pathogen (Aeromonas hydrophila) us- ing the British Society for Antimicrobial Chemotherapy (BSAC) standardized disc sus- ceptibility test method. The activity was mea- sured in terms of zone of inhibition in mm. Results: The acidic mucus extracts exhibited a bactericidal activity and inhibited the growth of Klebsiella pneumoniae, Pseudomonas aeruginosa and Bacillus subtilis while aqueous and crude ex- tract showed no bactericidal activity for any of the human pathogens tested. Further test against Fish pathogen Aeromonas hydrophila showed that the aqueous and crude extracts are capable of inhibiting the growth of the pathogen, demonstrating the presence of antimicrobial agents and the role of Fish mucus in antimicro- bial protection. Conclusions: The present results suggest that the mucus extracts of Snakehead Fish Chan- na striatus may be a potential source of antimi- crobial agents for human and Fish pathogens.
J Y Liu - One of the best experts on this subject based on the ideXlab platform.
-
first case of aeromonas schubertii infection in the freshwater cultured Snakehead Fish ophiocephalus argus cantor in china
Journal of Fish Diseases, 2012Co-Authors: J Y LiuAbstract:An epizootic in Snakehead Fish, Ophiocephalus argus, in earthen ponds in Xianning, Hubei Province, central China, from June to August 2009 was found to be caused by Aeromonas schubertii. The cumulative mortality within 40 days was 45%, and the diseased Fish were 18 months old and 35-45 cm in length. Multiple, ivory-white, firm nodules, 0.5-1 mm in diameter, were scattered throughout the kidney. Blood clots, 3-5 mm in diameter, were found in the liver. This is a disease frequently found in cultured Snakehead throughout China. Isolated bacteria were Gram negative, facultatively anaerobic, motile, short rod-shaped, with a length of 0.3-1.0 μm. Morphological and biochemical tests, as well as phylogenetic analysis derived from 16S rRNA, gyrB, rpoD and dnaJ gene sequencing all strongly indicated that these Snakehead isolates are identical to A. schubertii. In addition, the isolates possessed two plasmids: 5.0 kb and 10.0 kb. Antibiotic sensitivity testing of the isolates was carried out by the standard Kirby-Bauer disc diffusion method. Experimental infection assays were conducted, and pathogenicity (by intraperitoneal injection) was demonstrated in Snakehead fingerlings and zebraFish, Brachydanio rerio (Hamilton).
Alexander Chong Shuchien - One of the best experts on this subject based on the ideXlab platform.
-
molecular cloning and ontogenic mrna expression of fatty acid desaturase in the carnivorous striped Snakehead Fish channa striata
Comparative Biochemistry and Physiology A-molecular & Integrative Physiology, 2011Co-Authors: Annette Jayaram, Sairatul Dahlianis Ishak, Yeeling Enyu, Mengkiat Kuah, Kahloon Wong, Alexander Chong ShuchienAbstract:Abstract There is very little information on the capacity of freshwater carnivorous Fish to biosynthesize highly unsaturated fatty acids (HUFA). The striped Snakehead Fish (Channa striata) is a carnivorous species cultured inland of several Southeast Asian countries due to its pharmaceutical properties in wound healing enhancement. We described here the full-length cDNA cloning of a striped Snakehead fatty acid desaturase (fads), which is responsible for desaturation of unsaturated fatty acids in the HUFA biosynthesis. Bioinformatics analysis reveals a protein coding region with length of 445 amino acids containing all characteristic features of desaturase enzyme, including a cytochrome b5-domain with the heme-binding motif, two transmembrane domains and three histidine-rich regions. The striped Snakehead fads amino acid sequence shares high similarity with known fads of other teleosts. The mRNA expression of striped Snakehead fads also showed an ontogenic-related increase in expression in 0–20 days after hatch larva. Using ISH, we localized the presence of fads in larva brain, liver and intestinal tissues.
