The Experts below are selected from a list of 318 Experts worldwide ranked by ideXlab platform
Arkadiusz Orzechowski - One of the best experts on this subject based on the ideXlab platform.
-
protective effect of Sodium Ascorbate on efficacy of intramuscular transplantation of autologous muscle derived cells
Muscle & Nerve, 2012Co-Authors: Urszula Bartoszukbruzzone, Anna Burdzinska, Arkadiusz Orzechowski, Zdzislaw KlosAbstract:Department of Cell Ultrastructure, Mossakowski Medical Research Centre, Polish Academy of Sciences, Pawinskiego 5,02-106 Warsaw, PolandAccepted 19 July 2011ABSTRACT: Introduction: The possible reason for eliminationof myogenic cells after transplantation is inflammation at theinjection site associated with oxidative stress. The aim of thisstudy was to determine whether preconditioning of muscle-derived cells with an antioxidant, Sodium Ascorbate, can influ-ence the fate of transplanted cells. Methods: Autologous trans-plantation of muscle-derived cells was performed in rabbits.Isolated cells were identified, lipofected with b-galactosidase,preincubated or not with Sodium Ascorbate, and injected intra-muscularly. Results: Two weeks after autologous transplanta-tion in the edge of a previous muscle defect, donor cells formedmultinucleated young myotubes. Pretreatment of cells withSodium Ascorbate before injection resulted in a significantincrease of donor cells at the injection site 2 weeks after trans-fer. Conclusions: These results show that: (1) preincubationwith antioxidant can increase the efficacy of myogenic celltransplantation; and (2) oxidative stress may play a role in elimi-nation of cells after autologous transplantation.
-
Protective effect of Sodium Ascorbate on efficacy of intramuscular transplantation of autologous muscle‐derived cells
Muscle & Nerve, 2011Co-Authors: Urszula Bartoszuk-bruzzone, Anna Burdzinska, Arkadiusz OrzechowskiAbstract:Department of Cell Ultrastructure, Mossakowski Medical Research Centre, Polish Academy of Sciences, Pawinskiego 5,02-106 Warsaw, PolandAccepted 19 July 2011ABSTRACT: Introduction: The possible reason for eliminationof myogenic cells after transplantation is inflammation at theinjection site associated with oxidative stress. The aim of thisstudy was to determine whether preconditioning of muscle-derived cells with an antioxidant, Sodium Ascorbate, can influ-ence the fate of transplanted cells. Methods: Autologous trans-plantation of muscle-derived cells was performed in rabbits.Isolated cells were identified, lipofected with b-galactosidase,preincubated or not with Sodium Ascorbate, and injected intra-muscularly. Results: Two weeks after autologous transplanta-tion in the edge of a previous muscle defect, donor cells formedmultinucleated young myotubes. Pretreatment of cells withSodium Ascorbate before injection resulted in a significantincrease of donor cells at the injection site 2 weeks after trans-fer. Conclusions: These results show that: (1) preincubationwith antioxidant can increase the efficacy of myogenic celltransplantation; and (2) oxidative stress may play a role in elimi-nation of cells after autologous transplantation.
-
preincubation with bfgf but not Sodium Ascorbate improves efficiency of autologous transplantation of muscle derived cells into urethral wall
Urology, 2009Co-Authors: Anna Burdzinska, Arkadiusz Orzechowski, Urszula BartoszukAbstract:Objectives Myogenic cell therapy is considered a new method to treat urinary incontinence. One of the crucial limitations in cellular transplantation is poor cell survival after cell transfer. Preconditioning of cells before transfer is a new approach in improving the efficacy of graft. Methods In this study, autologous cell transplantation was performed on a rat model. Muscle-derived cells were isolated, identified with immunofluorescence and immunoblotting, preincubated or not with 10 ng/mL of basic fibroblast growth factor (bFGF) or 0.1 mM of Sodium Ascorbate for 24 hours, and transfected with the β-galactosidase encoding gene using lipofectamine. Cell suspension was injected into the urethral wall. The tissue was collected 2 weeks after injection. Results The presence of transplanted cells was confirmed by X-Gal staining. Moreover, the effects of preincubation with either Sodium Ascorbate or bFGF 24 hours before transfer were evaluated. A semiqualitative analysis using chlorophenol-red-β-d-galactopyranoside substrate demonstrated that preincubation with Ascorbate had no effect on the number of transplanted cells but that bFGF significantly increased the amount of cells in the engrafted tissue by 46% ( P Conclusions Preconditioning of myogenic cells with bFGF should be seriously considered as a new tool for improving cell viability after cell transfer in the treatment of urinary incontinence.
Manuel S Thomas - One of the best experts on this subject based on the ideXlab platform.
-
evaluation and comparison of bond strength to 10 carbamide peroxide bleached enamel following the application of 10 and 25 Sodium Ascorbate and alpha tocopherol solutions an in vitro study
Journal of Conservative Dentistry, 2013Co-Authors: Asha Thapa, Manuel S ThomasAbstract:Aim: To evaluate and compare composite bond strength to carbamide peroxide bleached enamel following the application of 10% and 25% Sodium Ascorbate and alpha-tocopherol solutions. Materials and Methods: Sixty premolars were divided into six groups. Groups I and VI served as unbleached and bleached controls respectively. Groups II, III, IV and V served as the experimental groups and were subjected to 10% carbamide peroxide bleaching followed by 10 min application of 10% and 25% Sodium Ascorbate and 10% and 25% alpha-tocopherol solutions, respectively. Following composite bonding, shear bond strength was determined and the results were analyzed using ANOVA and Tukey highest significant difference test. Results: Only Group IV showed significantly lower bond strength when compared to Group I (unbleached control). When compared to Group VI (bleached control), except Group IV, groups II, III and V showed significantly higher bond strength. However, there was no statistically significant difference between the experimental groups corresponding to 10% and 25% and similar concentrations of Sodium Ascorbate and alpha-tocopherol solutions. Conclusion: Following 10% carbamide peroxide bleaching, except 10% alpha tocopherol, 10 min application of 10% and 25% Sodium Ascorbate and 25% alpha-tocopherol solutions significantly improves the shear bond strength of composite resin to enamel.
-
Evaluation and comparison of bond strength to 10% carbamide peroxide bleached enamel following the application of 10% and 25% Sodium Ascorbate and alpha-tocopherol solutions: An in vitro study
Journal of Conservative Dentistry, 2013Co-Authors: Asha Thapa, Manuel S ThomasAbstract:Aim: To evaluate and compare composite bond strength to carbamide peroxide bleached enamel following the application of 10% and 25% Sodium Ascorbate and alpha-tocopherol solutions. Materials and Methods: Sixty premolars were divided into six groups. Groups I and VI served as unbleached and bleached controls respectively. Groups II, III, IV and V served as the experimental groups and were subjected to 10% carbamide peroxide bleaching followed by 10 min application of 10% and 25% Sodium Ascorbate and 10% and 25% alpha-tocopherol solutions, respectively. Following composite bonding, shear bond strength was determined and the results were analyzed using ANOVA and Tukey highest significant difference test. Results: Only Group IV showed significantly lower bond strength when compared to Group I (unbleached control). When compared to Group VI (bleached control), except Group IV, groups II, III and V showed significantly higher bond strength. However, there was no statistically significant difference between the experimental groups corresponding to 10% and 25% and similar concentrations of Sodium Ascorbate and alpha-tocopherol solutions. Conclusion: Following 10% carbamide peroxide bleaching, except 10% alpha tocopherol, 10 min application of 10% and 25% Sodium Ascorbate and 25% alpha-tocopherol solutions significantly improves the shear bond strength of composite resin to enamel.
Peter Parashos - One of the best experts on this subject based on the ideXlab platform.
-
effect of Sodium Ascorbate on resin bonding to Sodium perborate bleached dentin
Operative Dentistry, 2014Co-Authors: M Yoon, Michael F. Burrow, Rebecca Wong, Peter ParashosAbstract:Clinical Relevance Resin dentin bonding can be performed immediately after Sodium perborate bleaching without the need for Sodium Ascorbate pretreatment; however, the two-step self-etching adhesive performed better than the all-in-one system. The all-in-one adhesive, Xeno IV, exhibited significantly lower microshear bond strength when bonded to positions associated with larger-diameter dentinal tubules.
-
Effect of Sodium Ascorbate on resin bonding to Sodium perborate-bleached dentin.
Operative Dentistry, 2013Co-Authors: M Yoon, Michael F. Burrow, Rebecca Wong, Peter ParashosAbstract:Clinical Relevance Resin dentin bonding can be performed immediately after Sodium perborate bleaching without the need for Sodium Ascorbate pretreatment; however, the two-step self-etching adhesive...
Anna Burdzinska - One of the best experts on this subject based on the ideXlab platform.
-
protective effect of Sodium Ascorbate on efficacy of intramuscular transplantation of autologous muscle derived cells
Muscle & Nerve, 2012Co-Authors: Urszula Bartoszukbruzzone, Anna Burdzinska, Arkadiusz Orzechowski, Zdzislaw KlosAbstract:Department of Cell Ultrastructure, Mossakowski Medical Research Centre, Polish Academy of Sciences, Pawinskiego 5,02-106 Warsaw, PolandAccepted 19 July 2011ABSTRACT: Introduction: The possible reason for eliminationof myogenic cells after transplantation is inflammation at theinjection site associated with oxidative stress. The aim of thisstudy was to determine whether preconditioning of muscle-derived cells with an antioxidant, Sodium Ascorbate, can influ-ence the fate of transplanted cells. Methods: Autologous trans-plantation of muscle-derived cells was performed in rabbits.Isolated cells were identified, lipofected with b-galactosidase,preincubated or not with Sodium Ascorbate, and injected intra-muscularly. Results: Two weeks after autologous transplanta-tion in the edge of a previous muscle defect, donor cells formedmultinucleated young myotubes. Pretreatment of cells withSodium Ascorbate before injection resulted in a significantincrease of donor cells at the injection site 2 weeks after trans-fer. Conclusions: These results show that: (1) preincubationwith antioxidant can increase the efficacy of myogenic celltransplantation; and (2) oxidative stress may play a role in elimi-nation of cells after autologous transplantation.
-
Protective effect of Sodium Ascorbate on efficacy of intramuscular transplantation of autologous muscle‐derived cells
Muscle & Nerve, 2011Co-Authors: Urszula Bartoszuk-bruzzone, Anna Burdzinska, Arkadiusz OrzechowskiAbstract:Department of Cell Ultrastructure, Mossakowski Medical Research Centre, Polish Academy of Sciences, Pawinskiego 5,02-106 Warsaw, PolandAccepted 19 July 2011ABSTRACT: Introduction: The possible reason for eliminationof myogenic cells after transplantation is inflammation at theinjection site associated with oxidative stress. The aim of thisstudy was to determine whether preconditioning of muscle-derived cells with an antioxidant, Sodium Ascorbate, can influ-ence the fate of transplanted cells. Methods: Autologous trans-plantation of muscle-derived cells was performed in rabbits.Isolated cells were identified, lipofected with b-galactosidase,preincubated or not with Sodium Ascorbate, and injected intra-muscularly. Results: Two weeks after autologous transplanta-tion in the edge of a previous muscle defect, donor cells formedmultinucleated young myotubes. Pretreatment of cells withSodium Ascorbate before injection resulted in a significantincrease of donor cells at the injection site 2 weeks after trans-fer. Conclusions: These results show that: (1) preincubationwith antioxidant can increase the efficacy of myogenic celltransplantation; and (2) oxidative stress may play a role in elimi-nation of cells after autologous transplantation.
-
preincubation with bfgf but not Sodium Ascorbate improves efficiency of autologous transplantation of muscle derived cells into urethral wall
Urology, 2009Co-Authors: Anna Burdzinska, Arkadiusz Orzechowski, Urszula BartoszukAbstract:Objectives Myogenic cell therapy is considered a new method to treat urinary incontinence. One of the crucial limitations in cellular transplantation is poor cell survival after cell transfer. Preconditioning of cells before transfer is a new approach in improving the efficacy of graft. Methods In this study, autologous cell transplantation was performed on a rat model. Muscle-derived cells were isolated, identified with immunofluorescence and immunoblotting, preincubated or not with 10 ng/mL of basic fibroblast growth factor (bFGF) or 0.1 mM of Sodium Ascorbate for 24 hours, and transfected with the β-galactosidase encoding gene using lipofectamine. Cell suspension was injected into the urethral wall. The tissue was collected 2 weeks after injection. Results The presence of transplanted cells was confirmed by X-Gal staining. Moreover, the effects of preincubation with either Sodium Ascorbate or bFGF 24 hours before transfer were evaluated. A semiqualitative analysis using chlorophenol-red-β-d-galactopyranoside substrate demonstrated that preincubation with Ascorbate had no effect on the number of transplanted cells but that bFGF significantly increased the amount of cells in the engrafted tissue by 46% ( P Conclusions Preconditioning of myogenic cells with bFGF should be seriously considered as a new tool for improving cell viability after cell transfer in the treatment of urinary incontinence.
S De Smet - One of the best experts on this subject based on the ideXlab platform.
-
effect of Sodium Ascorbate and Sodium nitrite on protein and lipid oxidation in dry fermented sausages
Meat Science, 2016Co-Authors: Alberto Berardo, H De Maere, Despoina Angeliki Stavropoulou, Tine Rysman, Frederic Leroy, S De SmetAbstract:The effects of Sodium nitrite and Ascorbate on lipid and protein oxidation were studied during the ripening process of dry fermented sausages. Samples were taken at day 0, 2, 8, 14, 21 and 28 of ripening to assess lipid (malondialdehyde) and protein (carbonyls and sulfhydryl groups) oxidation. Sodium Ascorbate and nitrite were separately able to reduce the formation of malondialdehyde. Their combined addition resulted in higher amounts of carbonyl compounds compared to their separate addition or the treatment without any of both compounds. Moreover, Sodium nitrite limited the formation of γ-glutamic semialdehyde whereas Sodium Ascorbate showed a pro-oxidant effect. A loss of thiol groups was observed during ripening, which was not affected by the use of Sodium Ascorbate nor Sodium nitrite. In conclusion, Sodium nitrite and Ascorbate affected protein and lipid oxidation in different manners. The possible pro-oxidant effect of their combined addition on carbonyl formation might influence the technological and sensory properties of these products.
