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Ju Chu - One of the best experts on this subject based on the ideXlab platform.
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Kinetic analysis of Sodium Gluconate production by Aspergillus niger with different inlet oxygen concentrations.
Bioprocess and biosystems engineering, 2018Co-Authors: Xiwei Tian, Haifeng Hang, Wei Zhao, Yingping Zhuang, Shen Yuting, Ju ChuAbstract:To further understand fermentation kinetics of Sodium Gluconate (SG) production by Aspergillus niger with different inlet oxygen concentrations, logistic model for cell growth and two-step models for SG production and glucose consumption were established. The results demonstrated that the maximum specific growth rate (µm) presented exponential relationship with inlet oxygen concentration and the maximum biomass (Xm) exhibited linear increase. In terms of SG production, two-step model with Luedeking-Piret equation during growth phase and oxygen-dependent equation during stationary phase could well fit the experimental data. Notably, high inlet oxygen concentration exponentially improved SG yield (YP/S), whereas biomass yield to glucose (YX/S) and cell maintenance coefficient (m) were almost independent on inlet oxygen concentration, indicating that high oxygen supply enhancing SG synthesis not only functioning as a substrate directly, but also regulating glucose metabolism towards SG formation. Finally, the applicability and predictability of the proposed models were further validated by additional experiments.
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Influence of initial glucose concentration on seed culture of Sodium Gluconate production by Aspergillus niger
Bioresources and Bioprocessing, 2017Co-Authors: Xiu Liu, Haifeng Hang, Yonghong Wang, Xiwei Tian, Wei Zhao, Ju ChuAbstract:BackgroundIn general, high-quality seed is the prerequisite of an efficient bioprocess. However, in terms of Sodium Gluconate production by Aspergillus niger, reports have seldom focused on seed culture with rational optimization by process analysis technology, especially for carbon source effects. In this study, based on the online physiological parameter of oxygen uptake rate (OUR), and intracellular metabolite profiling, as well as cell morphology analysis, the effects of different initial glucose concentrations on seed culture by A. niger were investigated.ResultsThe optimum initial glucose concentration was 300 g/L, corresponding to 1900 mOsm/kg, with OUR level about 70% higher than other conditions. Besides, the cells from optimized seed culture accumulated more osmoprotectants of alanine and glutamate. Interestingly, high glucose concentration could induce glucose oxidase (GOD) activity possibly by affecting the synthesis of histidine, one key component of active site of GOD. Prominently, the fermentation yield using the optimized seed culture was up to 1.198 g/g, 99% of the theoretical value, which was the best in literature.ConclusionThe initial glucose concentration appropriately 300 g/L in seed cultivation was determined to be the most optimal. Further, this study would be helpful for guiding Sodium Gluconate production on industrial scale.
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Influence of initial glucose concentration on seed culture of Sodium Gluconate production by Aspergillus niger
Bioresources and Bioprocessing, 2017Co-Authors: Liu Xiu, Haifeng Hang, Yonghong Wang, Xiwei Tian, Wei Zhao, Ju ChuAbstract:In general, high-quality seed is the prerequisite of an efficient bioprocess. However, in terms of Sodium Gluconate production by Aspergillus niger, reports have seldom focused on seed culture with rational optimization by process analysis technology, especially for carbon source effects. In this study, based on the online physiological parameter of oxygen uptake rate (OUR), and intracellular metabolite profiling, as well as cell morphology analysis, the effects of different initial glucose concentrations on seed culture by A. niger were investigated. The optimum initial glucose concentration was 300 g/L, corresponding to 1900 mOsm/kg, with OUR level about 70% higher than other conditions. Besides, the cells from optimized seed culture accumulated more osmoprotectants of alanine and glutamate. Interestingly, high glucose concentration could induce glucose oxidase (GOD) activity possibly by affecting the synthesis of histidine, one key component of active site of GOD. Prominently, the fermentation yield using the optimized seed culture was up to 1.198 g/g, 99% of the theoretical value, which was the best in literature. The initial glucose concentration appropriately 300 g/L in seed cultivation was determined to be the most optimal. Further, this study would be helpful for guiding Sodium Gluconate production on industrial scale.
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Oxygen-enriched fermentation of Sodium Gluconate by Aspergillus niger and its impact on intracellular metabolic flux distributions
Bioprocess and biosystems engineering, 2017Co-Authors: Shen Yuting, Haifeng Hang, Xiwei Tian, Wei Zhao, Ju ChuAbstract:Different concentrations of oxygen-enriched air were utilized for Sodium Gluconate (SG) fermentation by Aspergillus niger. The fermentation time shortened from 20 to 15.5 h due to the increase of volumetric oxygen transfer coefficient (KLa) and the formation of more dispersed mycelia when inlet oxygen concentration ascended from 21 to 32%. According to metabolic flux analysis, during the growth phase, extracellular glucose for SG synthesis accounted for 79.0 and 85.3% with air and oxygen-enriched air (25%), respectively, whereas the proportions were 89.4 and 93.0% in the stationary phase. Intracellular glucose consumption decreased in oxygen-enriched fermentation, as cell respiration was more high-efficiently performed. Metabolic profiling indicated that most intermediates in TCA cycle and EMP pathway had smaller pool sizes in oxygen-enriched fermentations. Moreover, the main by-product of citric acid dramatically decreased from 1.36 to 0.34 g L−1 in oxygen-enriched fermentation. And the Sodium Gluconate yield increased from 0.856 to 0.903 mol mol−1.
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high efficiency cell recycle continuous Sodium Gluconate production by aspergillus niger using on line physiological parameters association analysis to regulate feed rate rationally
Bioresource Technology, 2016Co-Authors: Zejian Wang, Ju Chu, Wei Zhao, Yingping Zhuang, Siliang ZhangAbstract:Abstract In this paper, a system of cell-recycle continuous fermentation for Sodium Gluconate (SG) production by Aspergillus niger ( A. niger ) was established. Based on initial continuous fermentation result (100.0 h) with constant feed rate, an automatic feedback strategy to regulate feed rate using on-line physiological parameters (OUR and DO) was proposed and applied successfully for the first time in the improved continuous fermentation (240.5 h). Due to less auxiliary time, highest SG production rate (31.05 ± 0.29 g L −1 h −1 ) and highest yield (0.984 ± 0.067 mol mol −1 ), overall SG production capacity (975.8 ± 5.8 g h −1 ) in 50-L fermentor of improved continuous fermentation increased more than 300.0% compared to that of batch fermentation. Improvement of mass transfer and dispersed mycelia morphology were the two major reasons responsible for the high SG production rate. This system had been successfully applied to industrial fermentation and SG production was greatly improved.
R Touir - One of the best experts on this subject based on the ideXlab platform.
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Eco-friendly Sodium Gluconate and triSodium citrate inhibitors for low carbon steel in simulated cooling water system: Theoretical study and molecular dynamic simulations
Journal of Molecular Liquids, 2020Co-Authors: Goncagül Serdaroğlu, Savaş Kaya, R TouirAbstract:Abstract The work presented the corrosion inhibition potency of the Sodium Gluconate and triSodium citrate against the corrosion of low carbon steel and revealed that the TSC can be more preferment than the SG molecule. In addition to experimental investigation of the corrosion inhibition capacity of the SG and TSC molecules, the quantum chemical calculations were performed by B3LYP, B3LYP-GD3BJ and M06HF functionals at three basis sets that are 6-31g(d,p), 6-311g(d,p) and 6-311++g(3df, 2pd) basis sets. The global hardness values revealed that the TSC molecule could be preferable to the SG molecule in using the design of the corrosion inhibitor materials because the SG (2.81 eV) was calculated a harder molecule than the TSC (2.70 eV). Molecular dynamic simulations were performed to investigate metal-inhibitor interactions.
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Synergistic corrosion protection for galvanized steel in 3.0% NaCl solution by Sodium Gluconate and cationic surfactant
Journal of Molecular Liquids, 2016Co-Authors: M. A. Azaroual, R Touir, El Fadil El Harrak, A. Rochdi, M. Ebn TouhamiAbstract:Abstract The influence of Sodium Gluconate (SG), cetyltrimethylammonium bromide (CTAB) and their mixture on galvanized steel corrosion in 3.0% NaCl solution was investigated by using electrochemical measurement coupled with scanning electron microscopy (SEM). The polarization measurements indicated that SG, CTAB and their mixture act as cathodic-type inhibitors. Indeed, a synergistic effect was found between SG and CTAB. In fact, the inhibition efficiency of the mixture reached 94% at 10− 3 M of SG with 2.74 × 10− 5 M of CTAB. In addition, this inhibition efficiency improved remarkably with immersion time and temperature. The SEM observations and energy dispersive X-ray (EDX) analysis indicated that the surface homogeneity increases with mixture addition. Finally, the thermodynamic parameters were determined and discussed.
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Study of the mechanism action of Sodium Gluconate used for the protection of scale and corrosion in cooling water system
Journal of Saudi Chemical Society, 2014Co-Authors: R Touir, N. Dkhireche, Mohamed Ebn Touhami, Mohamed El Bakri, Abdel Hadi Rochdi, R.a. BelakhmimaAbstract:Abstract This work based on the mechanism action study of Sodium Gluconate (SG) for ordinary mild steel used for cooling water system treatment. In the first time, we evaluated the temperature effect on the scale inhibition of SG using statistic scale inhibition method. Result showed that the inhibition efficiency became more important with increasing temperature, at great concentration (10 −2 and 10 −3 M). This can be explained by forming of stable complex SG–Ca 2+ . In the second time, the present work focuses on the study of operational parameters and corrosion products effect on SG performance using potentiodynamic polarization and electrochemical impedance spectroscopic method. The obtained results show that SG is a very good inhibitor for corrosion and scale and remains effective in the presence of corrosion products. For this study we were proposed a mechanism action for SG on metallic surface. In addition, the SG keeps its effectiveness in a more aggressive medium such as 3% NaCl. Finally, to complete the formulation, we added a not oxidizing biocide (CTAB) to SG. The results obtained show that SG remains its effective.
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Sodium Gluconate as corrosion and scale inhibitor of ordinary steel in simulated cooling water
Corrosion Science, 2008Co-Authors: R Touir, M Cenoui, El M Bakri, Ebn M TouhamiAbstract:Abstract The effect of Sodium Gluconate anion (SG) on the corrosion and scale inhibition of ordinary steel in simulated cooling water has been studied using weight loss, polarisation curves, electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM) techniques. SG was studied in concentration from 10 −4 M to 10 −1 M. Results obtained reveal that SG perform excellently as corrosion and scaling inhibitor for ordinary steel in simulated cooling water. An increase of SG concentration leads to the increase of the corrosion potential towards the positive direction. The inhibition efficiency was a low temperature dependence. The inhibitor mechanism was treated as an adsorption process according to Langmuir adsorption isotherm. The SEM/EDAX data show that was a corrosion and scale inhibitor.
Jian Yang - One of the best experts on this subject based on the ideXlab platform.
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nagdf4 yb3 er3 nagdf4 nd3 Sodium Gluconate multifunctional and biocompatible ultrasmall core shell nanohybrids for ucl mr ct multimodal imaging
ACS Applied Materials & Interfaces, 2015Co-Authors: Lingjie Meng, Yuzhong Chen, Yanke Chen, Chen Huang, Jin Shang, Ruifeng Wang, Youmin Guo, Jian YangAbstract:Multimodal bioimaging nanoparticles by integrating diverse imaging ingredients into one system, represent a class of emerging advanced materials that provide more comprehensive and accurate clinical diagnostics than conventional contrast agents. Here monodisperse and biocompatible core–shell nanoparticles, NaGdF4: Yb3+/Er3+@NaGdF4:Nd@Sodium-Gluconate (termed as GNa-Er@Nd), with about 26 nm in diameter were successfully prepared by a facile two step reactions in high boiling solvents, and followed a ligand exchange process with Sodium Gluconate. The resulting GNa-Er@Nd nanoparticles were well characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectra (FTIR), and zeta potentials. These nanohybrids present brightly dual-wavelength excited upconversion luminescence (UCL) under both 980 and 793 nm laser because of the synergistic effect of Yb3+/Er3+ and Nd3+. They also exhibited excellent relaxivity parameters (r1) in magnetic resonance imaging (MRI) an...
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NaGdF4:Yb3+/Er3+@NaGdF4:Nd3+@Sodium-Gluconate: Multifunctional and Biocompatible Ultrasmall Core–Shell Nanohybrids for UCL/MR/CT Multimodal Imaging
ACS applied materials & interfaces, 2015Co-Authors: Lingjie Meng, Yuzhong Chen, Yanke Chen, Chen Huang, Jin Shang, Ruifeng Wang, Youmin Guo, Jian YangAbstract:Multimodal bioimaging nanoparticles by integrating diverse imaging ingredients into one system, represent a class of emerging advanced materials that provide more comprehensive and accurate clinical diagnostics than conventional contrast agents. Here monodisperse and biocompatible core–shell nanoparticles, NaGdF4: Yb3+/Er3+@NaGdF4:Nd@Sodium-Gluconate (termed as GNa-Er@Nd), with about 26 nm in diameter were successfully prepared by a facile two step reactions in high boiling solvents, and followed a ligand exchange process with Sodium Gluconate. The resulting GNa-Er@Nd nanoparticles were well characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectra (FTIR), and zeta potentials. These nanohybrids present brightly dual-wavelength excited upconversion luminescence (UCL) under both 980 and 793 nm laser because of the synergistic effect of Yb3+/Er3+ and Nd3+. They also exhibited excellent relaxivity parameters (r1) in magnetic resonance imaging (MRI) an...
Wei Zhao - One of the best experts on this subject based on the ideXlab platform.
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Kinetic analysis of Sodium Gluconate production by Aspergillus niger with different inlet oxygen concentrations.
Bioprocess and biosystems engineering, 2018Co-Authors: Xiwei Tian, Haifeng Hang, Wei Zhao, Yingping Zhuang, Shen Yuting, Ju ChuAbstract:To further understand fermentation kinetics of Sodium Gluconate (SG) production by Aspergillus niger with different inlet oxygen concentrations, logistic model for cell growth and two-step models for SG production and glucose consumption were established. The results demonstrated that the maximum specific growth rate (µm) presented exponential relationship with inlet oxygen concentration and the maximum biomass (Xm) exhibited linear increase. In terms of SG production, two-step model with Luedeking-Piret equation during growth phase and oxygen-dependent equation during stationary phase could well fit the experimental data. Notably, high inlet oxygen concentration exponentially improved SG yield (YP/S), whereas biomass yield to glucose (YX/S) and cell maintenance coefficient (m) were almost independent on inlet oxygen concentration, indicating that high oxygen supply enhancing SG synthesis not only functioning as a substrate directly, but also regulating glucose metabolism towards SG formation. Finally, the applicability and predictability of the proposed models were further validated by additional experiments.
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Influence of initial glucose concentration on seed culture of Sodium Gluconate production by Aspergillus niger
Bioresources and Bioprocessing, 2017Co-Authors: Xiu Liu, Haifeng Hang, Yonghong Wang, Xiwei Tian, Wei Zhao, Ju ChuAbstract:BackgroundIn general, high-quality seed is the prerequisite of an efficient bioprocess. However, in terms of Sodium Gluconate production by Aspergillus niger, reports have seldom focused on seed culture with rational optimization by process analysis technology, especially for carbon source effects. In this study, based on the online physiological parameter of oxygen uptake rate (OUR), and intracellular metabolite profiling, as well as cell morphology analysis, the effects of different initial glucose concentrations on seed culture by A. niger were investigated.ResultsThe optimum initial glucose concentration was 300 g/L, corresponding to 1900 mOsm/kg, with OUR level about 70% higher than other conditions. Besides, the cells from optimized seed culture accumulated more osmoprotectants of alanine and glutamate. Interestingly, high glucose concentration could induce glucose oxidase (GOD) activity possibly by affecting the synthesis of histidine, one key component of active site of GOD. Prominently, the fermentation yield using the optimized seed culture was up to 1.198 g/g, 99% of the theoretical value, which was the best in literature.ConclusionThe initial glucose concentration appropriately 300 g/L in seed cultivation was determined to be the most optimal. Further, this study would be helpful for guiding Sodium Gluconate production on industrial scale.
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Influence of initial glucose concentration on seed culture of Sodium Gluconate production by Aspergillus niger
Bioresources and Bioprocessing, 2017Co-Authors: Liu Xiu, Haifeng Hang, Yonghong Wang, Xiwei Tian, Wei Zhao, Ju ChuAbstract:In general, high-quality seed is the prerequisite of an efficient bioprocess. However, in terms of Sodium Gluconate production by Aspergillus niger, reports have seldom focused on seed culture with rational optimization by process analysis technology, especially for carbon source effects. In this study, based on the online physiological parameter of oxygen uptake rate (OUR), and intracellular metabolite profiling, as well as cell morphology analysis, the effects of different initial glucose concentrations on seed culture by A. niger were investigated. The optimum initial glucose concentration was 300 g/L, corresponding to 1900 mOsm/kg, with OUR level about 70% higher than other conditions. Besides, the cells from optimized seed culture accumulated more osmoprotectants of alanine and glutamate. Interestingly, high glucose concentration could induce glucose oxidase (GOD) activity possibly by affecting the synthesis of histidine, one key component of active site of GOD. Prominently, the fermentation yield using the optimized seed culture was up to 1.198 g/g, 99% of the theoretical value, which was the best in literature. The initial glucose concentration appropriately 300 g/L in seed cultivation was determined to be the most optimal. Further, this study would be helpful for guiding Sodium Gluconate production on industrial scale.
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Oxygen-enriched fermentation of Sodium Gluconate by Aspergillus niger and its impact on intracellular metabolic flux distributions
Bioprocess and biosystems engineering, 2017Co-Authors: Shen Yuting, Haifeng Hang, Xiwei Tian, Wei Zhao, Ju ChuAbstract:Different concentrations of oxygen-enriched air were utilized for Sodium Gluconate (SG) fermentation by Aspergillus niger. The fermentation time shortened from 20 to 15.5 h due to the increase of volumetric oxygen transfer coefficient (KLa) and the formation of more dispersed mycelia when inlet oxygen concentration ascended from 21 to 32%. According to metabolic flux analysis, during the growth phase, extracellular glucose for SG synthesis accounted for 79.0 and 85.3% with air and oxygen-enriched air (25%), respectively, whereas the proportions were 89.4 and 93.0% in the stationary phase. Intracellular glucose consumption decreased in oxygen-enriched fermentation, as cell respiration was more high-efficiently performed. Metabolic profiling indicated that most intermediates in TCA cycle and EMP pathway had smaller pool sizes in oxygen-enriched fermentations. Moreover, the main by-product of citric acid dramatically decreased from 1.36 to 0.34 g L−1 in oxygen-enriched fermentation. And the Sodium Gluconate yield increased from 0.856 to 0.903 mol mol−1.
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high efficiency cell recycle continuous Sodium Gluconate production by aspergillus niger using on line physiological parameters association analysis to regulate feed rate rationally
Bioresource Technology, 2016Co-Authors: Zejian Wang, Ju Chu, Wei Zhao, Yingping Zhuang, Siliang ZhangAbstract:Abstract In this paper, a system of cell-recycle continuous fermentation for Sodium Gluconate (SG) production by Aspergillus niger ( A. niger ) was established. Based on initial continuous fermentation result (100.0 h) with constant feed rate, an automatic feedback strategy to regulate feed rate using on-line physiological parameters (OUR and DO) was proposed and applied successfully for the first time in the improved continuous fermentation (240.5 h). Due to less auxiliary time, highest SG production rate (31.05 ± 0.29 g L −1 h −1 ) and highest yield (0.984 ± 0.067 mol mol −1 ), overall SG production capacity (975.8 ± 5.8 g h −1 ) in 50-L fermentor of improved continuous fermentation increased more than 300.0% compared to that of batch fermentation. Improvement of mass transfer and dispersed mycelia morphology were the two major reasons responsible for the high SG production rate. This system had been successfully applied to industrial fermentation and SG production was greatly improved.
Yingping Zhuang - One of the best experts on this subject based on the ideXlab platform.
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Kinetic analysis of Sodium Gluconate production by Aspergillus niger with different inlet oxygen concentrations.
Bioprocess and biosystems engineering, 2018Co-Authors: Xiwei Tian, Haifeng Hang, Wei Zhao, Yingping Zhuang, Shen Yuting, Ju ChuAbstract:To further understand fermentation kinetics of Sodium Gluconate (SG) production by Aspergillus niger with different inlet oxygen concentrations, logistic model for cell growth and two-step models for SG production and glucose consumption were established. The results demonstrated that the maximum specific growth rate (µm) presented exponential relationship with inlet oxygen concentration and the maximum biomass (Xm) exhibited linear increase. In terms of SG production, two-step model with Luedeking-Piret equation during growth phase and oxygen-dependent equation during stationary phase could well fit the experimental data. Notably, high inlet oxygen concentration exponentially improved SG yield (YP/S), whereas biomass yield to glucose (YX/S) and cell maintenance coefficient (m) were almost independent on inlet oxygen concentration, indicating that high oxygen supply enhancing SG synthesis not only functioning as a substrate directly, but also regulating glucose metabolism towards SG formation. Finally, the applicability and predictability of the proposed models were further validated by additional experiments.
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high efficiency cell recycle continuous Sodium Gluconate production by aspergillus niger using on line physiological parameters association analysis to regulate feed rate rationally
Bioresource Technology, 2016Co-Authors: Zejian Wang, Ju Chu, Wei Zhao, Yingping Zhuang, Siliang ZhangAbstract:Abstract In this paper, a system of cell-recycle continuous fermentation for Sodium Gluconate (SG) production by Aspergillus niger ( A. niger ) was established. Based on initial continuous fermentation result (100.0 h) with constant feed rate, an automatic feedback strategy to regulate feed rate using on-line physiological parameters (OUR and DO) was proposed and applied successfully for the first time in the improved continuous fermentation (240.5 h). Due to less auxiliary time, highest SG production rate (31.05 ± 0.29 g L −1 h −1 ) and highest yield (0.984 ± 0.067 mol mol −1 ), overall SG production capacity (975.8 ± 5.8 g h −1 ) in 50-L fermentor of improved continuous fermentation increased more than 300.0% compared to that of batch fermentation. Improvement of mass transfer and dispersed mycelia morphology were the two major reasons responsible for the high SG production rate. This system had been successfully applied to industrial fermentation and SG production was greatly improved.
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effect of oxygen supply on the intracellular flux distribution and a two stage our control strategy for enhancing the yield of Sodium Gluconate production by aspergillus niger
Journal of Chemical Technology & Biotechnology, 2016Co-Authors: Kang-kang Ping, Ju Chu, Wei Zhao, Yingping Zhuang, Zejian Wang, Yonghong WangAbstract:BACKGROUND In this study, the performance of aerobic batch fermentation with Aspergillus niger producing Sodium Gluconate under different oxygen supply levels through adjusting the agitation rate were investigated. The response of glucose metabolism in A. niger to different oxygen uptake rate (OUR) levels has been studied in the present work. RESULTS Metabolic flux analysis demonstrated that the high oxygen supply condition was favorable for cell growth and initial Sodium Gluconate synthesis during the early fermentation phases. However, during the late stable phase, metabolic flux analysis indicated that a high yield of Sodium Gluconate production could be achieved at a medium OUR level of 55 ± 2.5 mmol L−1 h−1 as less flux was required for glucolysis and the TCA cycle. With a two-stage OUR control strategy, the final Sodium Gluconate yield of the batch fermentation was enhanced and reached 93.7% (mol vs mol), which was higher than those obtained using a high oxygen supply level throughout the whole fermentation process. CONCLUSIONS Metabolic flux analysis was successfully used in the present work, and the two-stage OUR control strategy increased the yield of Sodium Gluconate production to 93.7%. © 2015 Society of Chemical Industry
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a simple novel approach for real time monitoring of Sodium Gluconate production by on line physiological parameters in batch fermentation by aspergillus niger
Bioresource Technology, 2016Co-Authors: Zejian Wang, Ju Chu, Wei Zhao, Yingping ZhuangAbstract:In this paper, approach for real-time monitoring of Sodium Gluconate (SG) fermentation was established for the first time by the equations which can calculate real-time key-parameters by on-line physiological data. Based on this approach, limiting factors were found out in initial fermentation F1 and then step-wise agitation increase and improved medium recipe were proposed in fermentation F2 and F3, respectively. The highest average SG production rate (16.58±0.91 g L(-1) h(-1)) was achieved in fermentation F3, which was 104.2% and 48.0% higher than those in fermentation F1 and F2, respectively. Meanwhile, due to shorter fermentation period (decreased from 34 h to 18.7 h), lower biomass (about 1.5 g L(-1)) and less by-product accumulation, the overall yield of 0.943±0.012 (mol mol(-1)) in fermentation F3 increased more than 16.0% compared to fermentation F1. This approach had been successfully applied to industrial fermentation and greatly improved SG production.
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Power series kinetic model based on generalized stoichiometric equations for microbial production of Sodium Gluconate
Biochemical Engineering Journal, 2016Co-Authors: Wang Xinchao, Meijin Guo, Xuefeng Yan, Yingping ZhuangAbstract:Abstract Two main approaches are available for modeling of the fermentation process; these two are building an unstructured dynamic model and quantitative analysis of the metabolic network. The first one often employs several classic equations mostly derived from empirical knowledge and observation rather than mechanism knowledge. The second one is complicated because it requires sufficient biological information on cellular metabolic pathways. The objective of this article is to develop a kinetic model of the microbial production of Sodium Gluconate by using generalized stoichiometric equations and a typical enzyme kinetic structure called power series to overcome the shortcomings of previous methods. The proposed kinetic model can describe the microbial growth of fungus as well as the interaction among dissolved oxygen, fungal metabolism, and product formation. Six batches were selected from seven batches of experimental sample data for modeling and analysis. The fitting precision was acceptable. The key parameters were analyzed based on the model. The main advantage of this model is that it has a simple structure based on the mechanism and can describe the fermentation process with sufficient accuracy.
