The Experts below are selected from a list of 39909 Experts worldwide ranked by ideXlab platform
Jacob I. Sznajder - One of the best experts on this subject based on the ideXlab platform.
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Dopamine stimulates Sodium Transport and liquid clearance in rat lung epithelium.
American Journal of Respiratory and Critical Care Medicine, 1997Co-Authors: M. L. Barnard, W.g. Olivera, David M. Rutschman, Alejandro M. Bertorello, Adriana Katz, Jacob I. SznajderAbstract:Pulmonary edema clearance is driven primarily by active Sodium Transport out of the alveoli, mediated predominantly by apical Sodium channels and the basolateral NA,K-ATPase. We postulated that dopamine, analogous to its effects in other Transporting epithelia, could regulate these Sodium Transport mechanisms and affect lung liquid clearance. We therefore studied the effects of dopamine on Sodium Transport and liquid clearance in isolated perfused rat lungs. Instillation of dopamine into the airways caused a dose-dependent increase in liquid clearance from isolated rat lungs of up to 33% above control values at 10− 8 to 10− 4 M concentrations. 10− 6 M amiloride, which selectively inhibits apical Sodium channels, decreased basal liquid clearance by 34% but did not inhibit the dopamine-mediated stimulation of lung liquid clearance. Instillation of 10− 4 M amiloride into rat airways, which inhibits other Sodium Transport mechanisms non-selectively, decreased basal lung liquid clearance by 49% and inhibited t...
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Dopamine stimulates Sodium Transport and liquid clearance in rat lung epithelium.
American journal of respiratory and critical care medicine, 1997Co-Authors: M. L. Barnard, W.g. Olivera, David M. Rutschman, Alejandro M. Bertorello, A I Katz, Jacob I. SznajderAbstract:Pulmonary edema clearance is driven primarily by active Sodium Transport out of the alveoli, mediated predominantly by apical Sodium channels and the basolateral NA,K-ATPase. We postulated that dopamine, analogous to its effects in other Transporting epithelia, could regulate these Sodium Transport mechanisms and affect lung liquid clearance. We therefore studied the effects of dopamine on Sodium Transport and liquid clearance in isolated perfused rat lungs. Instillation of dopamine into the airways caused a dose-dependent increase in liquid clearance from isolated rat lungs of up to 33% above control values at 10(-8) to 10(-4) M concentrations. 10(-6) M amiloride, which selectively inhibits apical Sodium channels, decreased basal liquid clearance by 34% but did not inhibit the dopamine-mediated stimulation of lung liquid clearance. Instillation of 10(-4) M amiloride into rat airways, which inhibits other Sodium Transport mechanisms non-selectively, decreased basal lung liquid clearance by 49% and inhibited the dopamine-mediated stimulation of lung liquid clearance. Perfusion of rat lungs with 5 x 10(-4) M ouabain to specifically inhibit Na,K-ATPase reduced both basal clearance (by 55%) and the dopamine-stimulated increase in lung fluid clearance. Conceivably, the stimulation of lung liquid clearance by dopamine is due to a modulation of Na,K-ATPase in the pulmonary epithelium.
M. L. Barnard - One of the best experts on this subject based on the ideXlab platform.
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Dopamine stimulates Sodium Transport and liquid clearance in rat lung epithelium.
American Journal of Respiratory and Critical Care Medicine, 1997Co-Authors: M. L. Barnard, W.g. Olivera, David M. Rutschman, Alejandro M. Bertorello, Adriana Katz, Jacob I. SznajderAbstract:Pulmonary edema clearance is driven primarily by active Sodium Transport out of the alveoli, mediated predominantly by apical Sodium channels and the basolateral NA,K-ATPase. We postulated that dopamine, analogous to its effects in other Transporting epithelia, could regulate these Sodium Transport mechanisms and affect lung liquid clearance. We therefore studied the effects of dopamine on Sodium Transport and liquid clearance in isolated perfused rat lungs. Instillation of dopamine into the airways caused a dose-dependent increase in liquid clearance from isolated rat lungs of up to 33% above control values at 10− 8 to 10− 4 M concentrations. 10− 6 M amiloride, which selectively inhibits apical Sodium channels, decreased basal liquid clearance by 34% but did not inhibit the dopamine-mediated stimulation of lung liquid clearance. Instillation of 10− 4 M amiloride into rat airways, which inhibits other Sodium Transport mechanisms non-selectively, decreased basal lung liquid clearance by 49% and inhibited t...
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Dopamine stimulates Sodium Transport and liquid clearance in rat lung epithelium.
American journal of respiratory and critical care medicine, 1997Co-Authors: M. L. Barnard, W.g. Olivera, David M. Rutschman, Alejandro M. Bertorello, A I Katz, Jacob I. SznajderAbstract:Pulmonary edema clearance is driven primarily by active Sodium Transport out of the alveoli, mediated predominantly by apical Sodium channels and the basolateral NA,K-ATPase. We postulated that dopamine, analogous to its effects in other Transporting epithelia, could regulate these Sodium Transport mechanisms and affect lung liquid clearance. We therefore studied the effects of dopamine on Sodium Transport and liquid clearance in isolated perfused rat lungs. Instillation of dopamine into the airways caused a dose-dependent increase in liquid clearance from isolated rat lungs of up to 33% above control values at 10(-8) to 10(-4) M concentrations. 10(-6) M amiloride, which selectively inhibits apical Sodium channels, decreased basal liquid clearance by 34% but did not inhibit the dopamine-mediated stimulation of lung liquid clearance. Instillation of 10(-4) M amiloride into rat airways, which inhibits other Sodium Transport mechanisms non-selectively, decreased basal lung liquid clearance by 49% and inhibited the dopamine-mediated stimulation of lung liquid clearance. Perfusion of rat lungs with 5 x 10(-4) M ouabain to specifically inhibit Na,K-ATPase reduced both basal clearance (by 55%) and the dopamine-stimulated increase in lung fluid clearance. Conceivably, the stimulation of lung liquid clearance by dopamine is due to a modulation of Na,K-ATPase in the pulmonary epithelium.
Gerhard Giebisch - One of the best experts on this subject based on the ideXlab platform.
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relationship between Sodium Transport and intracellular atp in isolated perfused rabbit proximal convoluted tubule
American Journal of Physiology-renal Physiology, 1991Co-Authors: J S Beck, Raynald Laprade, Heimo Mairbaurl, Sylvie Breton, Gerhard GiebischAbstract:: The effect of alterations in Sodium Transport on cell ATP content and pH in the isolated perfused proximal convoluted tubule (PCT) of the rabbit was examined. Stimulating Sodium Transport by the addition of luminal glucose and alanine decreased cell ATP from 4.44 +/- 0.93 to 2.69 +/- 0.62 mM (n = 4), increased intracellular pH by 0.13 +/- 0.02 (n = 7), and increased cell volume by 0.10 +/- 0.02 nl/mm (n = 4). Blocking the Sodium pump with 10(-4) M strophanthidin in tubules in which Sodium Transport had been stimulated increased cell ATP from 2.04 +/- 0.24 to 2.42 +/- 0.32 mM (n = 6). In parallel experiments the same dose of strophanthidin depolarized the basolateral membrane from -52.6 +/- 1.9 to -6.4 +/- 1.6 mV, depolarized the transepithelial potential from -3.2 +/- 0.3 to -0.1 +/- 0.1 mV, and reduced the basolateral membrane potassium transference number from 0.47 to 0.26 indicating a reduction in basolateral potassium conductance. Since strophanthidin caused a cell alkalinization of 0.15 +/- 0.03, this latter effect cannot be due to changes of intracellular pH. Strophanthidin caused no change in cell volume over the period studied, suggesting that stretch-activated potassium channels are not involved either. Instead, potassium conductance inhibition may be the result of the closure of ATP-sensitive potassium channels. These same channels might thus be partly responsible for the increase in potassium conductance commonly observed during stimulation of Sodium Transport.
Maris P Apse - One of the best experts on this subject based on the ideXlab platform.
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Sodium Transport in plant cells
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2000Co-Authors: Eduardo Blumwald, Gilad S Aharon, Maris P ApseAbstract:AbstractSalinity limits plant growth and impairs agricultural productivity. There is a wide spectrum of plant responses to salinity that are defined by a range of adaptations at the cellular and the whole-plant levels, however, the mechanisms of Sodium Transport appear to be fundamentally similar. At the cellular level, Sodium ions gain entry via several plasma membrane channels. As cytoplasmic Sodium is toxic above threshold levels, it is extruded by plasma membrane Na+/H+ antiports that are energized by the proton gradient generated by the plasma membrane ATPase. Cytoplasmic Na+ may also be compartmentalized by vacuolar Na+/H+ antiports. These Transporters are energized by the proton gradient generated by the vacuolar H+-ATPase and H+-PPiase. Here, the mechanisms of Sodium entry, extrusion, and compartmentation are reviewed, with a discussion of recent progress on the cloning and characterization, directly in planta and in yeast, of some of the proteins involved in Sodium Transport
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Review Sodium Transport in plant cells
2000Co-Authors: Eduardo Blumwald, Gilad S Aharon, Maris P ApseAbstract:Salinity limits plant growth and impairs agricultural productivity. There is a wide spectrum of plant responses to salinity that are defined by a range of adaptations at the cellular and the whole-plant levels, however, the mechanisms of Sodium Transport appear to be fundamentally similar. At the cellular level, Sodium ions gain entry via several plasma membrane channels. As cytoplasmic Sodium is toxic above threshold levels, it is extruded by plasma membrane Na a /H a antiports that are energized by the proton gradient generated by the plasma membrane ATPase. Cytoplasmic Na a may also be compartmentalized by vacuolar Na a /H a antiports. These Transporters are energized by the proton gradient generated by the vacuolar H a -ATPase and H a -PPiase. Here, the mechanisms of Sodium entry, extrusion, and compartmentation are reviewed, with a discussion of recent progress on the cloning and characterization, directly in planta and in yeast, of some of the proteins involved in Sodium Transport. fl 2000 Elsevier Science B.V. All rights reserved.
Alejandro M. Bertorello - One of the best experts on this subject based on the ideXlab platform.
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Dopamine stimulates Sodium Transport and liquid clearance in rat lung epithelium.
American Journal of Respiratory and Critical Care Medicine, 1997Co-Authors: M. L. Barnard, W.g. Olivera, David M. Rutschman, Alejandro M. Bertorello, Adriana Katz, Jacob I. SznajderAbstract:Pulmonary edema clearance is driven primarily by active Sodium Transport out of the alveoli, mediated predominantly by apical Sodium channels and the basolateral NA,K-ATPase. We postulated that dopamine, analogous to its effects in other Transporting epithelia, could regulate these Sodium Transport mechanisms and affect lung liquid clearance. We therefore studied the effects of dopamine on Sodium Transport and liquid clearance in isolated perfused rat lungs. Instillation of dopamine into the airways caused a dose-dependent increase in liquid clearance from isolated rat lungs of up to 33% above control values at 10− 8 to 10− 4 M concentrations. 10− 6 M amiloride, which selectively inhibits apical Sodium channels, decreased basal liquid clearance by 34% but did not inhibit the dopamine-mediated stimulation of lung liquid clearance. Instillation of 10− 4 M amiloride into rat airways, which inhibits other Sodium Transport mechanisms non-selectively, decreased basal lung liquid clearance by 49% and inhibited t...
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Dopamine stimulates Sodium Transport and liquid clearance in rat lung epithelium.
American journal of respiratory and critical care medicine, 1997Co-Authors: M. L. Barnard, W.g. Olivera, David M. Rutschman, Alejandro M. Bertorello, A I Katz, Jacob I. SznajderAbstract:Pulmonary edema clearance is driven primarily by active Sodium Transport out of the alveoli, mediated predominantly by apical Sodium channels and the basolateral NA,K-ATPase. We postulated that dopamine, analogous to its effects in other Transporting epithelia, could regulate these Sodium Transport mechanisms and affect lung liquid clearance. We therefore studied the effects of dopamine on Sodium Transport and liquid clearance in isolated perfused rat lungs. Instillation of dopamine into the airways caused a dose-dependent increase in liquid clearance from isolated rat lungs of up to 33% above control values at 10(-8) to 10(-4) M concentrations. 10(-6) M amiloride, which selectively inhibits apical Sodium channels, decreased basal liquid clearance by 34% but did not inhibit the dopamine-mediated stimulation of lung liquid clearance. Instillation of 10(-4) M amiloride into rat airways, which inhibits other Sodium Transport mechanisms non-selectively, decreased basal lung liquid clearance by 49% and inhibited the dopamine-mediated stimulation of lung liquid clearance. Perfusion of rat lungs with 5 x 10(-4) M ouabain to specifically inhibit Na,K-ATPase reduced both basal clearance (by 55%) and the dopamine-stimulated increase in lung fluid clearance. Conceivably, the stimulation of lung liquid clearance by dopamine is due to a modulation of Na,K-ATPase in the pulmonary epithelium.
