The Experts below are selected from a list of 3288 Experts worldwide ranked by ideXlab platform

Shuhe Wei - One of the best experts on this subject based on the ideXlab platform.

Yihai Wang - One of the best experts on this subject based on the ideXlab platform.

  • Steroidal alkaloid glycosides and phenolics from the immature fruits of Solanum Nigrum.
    Fitoterapia, 2019
    Co-Authors: Limin Xiang, Yihai Wang
    Abstract:

    Abstract Solanum Nigrum L. (also called as European black nightshade) has been traditionally used as folk medicine and food in some regions. Phytochemical investigations of the immature fruits of S. Nigrum yielded five steroidal alkaloid glycosides (1–5), including an unprecedented nor-spirosolane type steroidal alkaloid with a five-membered ring A (1) and two novel spirosolane type steroidal alkaloid glycosides (2, 3), together with eight known phenolic compounds (6–13). Their structures were elucidated on the basis of spectroscopic and chemical methods, including IR, NMR, HR-ESI-MS, and GC analyses. Five steroidal alkaloid glycosides were tested for their potential antiproliferative effects against HL-60, U-937, Jurkat, K562, and HepG2 cell lines and inhibitory activities on nitric oxide (NO) production induced by lipopolysaccharide (LPS) in a macrophage cell line RAW 264.7. Compound 1 exhibited significant inhibition on NO production with an IC50 value of 23.4 ± 2.0 μM, compared to positive control indomethacin (IC50, 47.40 ± 4.50 μM). Compound 4 exhibited significant cytotoxicity against all tested cell lines.

  • anti inflammatory steroidal glycosides from the berries of Solanum Nigrum l european black nightshade
    Phytochemistry, 2018
    Co-Authors: Limin Xiang, Yihai Wang
    Abstract:

    Abstract Seven previously undescribed steroidal glycosides, along with three known congeners were isolated from the unripe berries of Solanum Nigrum L. (Solanaceae). Their structures were elucidated on basis of 1D and 2D NMR, HR-ESI-MS spectroscopic data and GC analysis after acid hydrolysis. The potential inhibitory effects on nitric oxide (NO) production induced by lipopolysaccharide in RAW 264.7 cell line and the anti-proliferative activities against five cancer cell lines (HL-60, U-937, Jurkat, K562 and HepG2) were evaluated. Seven compounds exhibited inhibition activities on NO production with IC50 values ranging from 11.33 to 49.35 μM. Structure-activity relationships of the isolated compounds were also discussed.

  • potential anti inflammatory steroidal saponins from the berries of Solanum Nigrum l european black nightshade
    Journal of Agricultural and Food Chemistry, 2017
    Co-Authors: Yihai Wang, Limin Xiang
    Abstract:

    Solanum Nigrum L. or European black nightshade (Solanum genus) is a common weed of crops and gardens. The berries and leaves of S. Nigrum L. are consumed as food or vegetable in some regions and reported to possess a range of biological activities. In this study, nine new steroidal saponins, solanigrosides Y1–Y9 (1–6, 10–12), together with seven known congeners, were isolated from the berries of S. Nigrum. Their potential inhibitory effects on nitric oxide (NO) and IL-6 and IL-1β production induced by lipopolysaccharide (LPS) in macrophages cell line RAW 264.7 were evaluated. Compound 1 exhibited significant inhibition on NO production with an IC50 value of 9.7 μM, and some compounds exhibited significant inhibition effects on the LPS-induced IL-6 and IL-1β production. These results suggest that the steroidal saponins from berries of S. Nigrum demonstrated pronounced anti-inflammatory activity and might be explored as a healthy benefit agent.

Limin Xiang - One of the best experts on this subject based on the ideXlab platform.

  • Steroidal alkaloid glycosides and phenolics from the immature fruits of Solanum Nigrum.
    Fitoterapia, 2019
    Co-Authors: Limin Xiang, Yihai Wang
    Abstract:

    Abstract Solanum Nigrum L. (also called as European black nightshade) has been traditionally used as folk medicine and food in some regions. Phytochemical investigations of the immature fruits of S. Nigrum yielded five steroidal alkaloid glycosides (1–5), including an unprecedented nor-spirosolane type steroidal alkaloid with a five-membered ring A (1) and two novel spirosolane type steroidal alkaloid glycosides (2, 3), together with eight known phenolic compounds (6–13). Their structures were elucidated on the basis of spectroscopic and chemical methods, including IR, NMR, HR-ESI-MS, and GC analyses. Five steroidal alkaloid glycosides were tested for their potential antiproliferative effects against HL-60, U-937, Jurkat, K562, and HepG2 cell lines and inhibitory activities on nitric oxide (NO) production induced by lipopolysaccharide (LPS) in a macrophage cell line RAW 264.7. Compound 1 exhibited significant inhibition on NO production with an IC50 value of 23.4 ± 2.0 μM, compared to positive control indomethacin (IC50, 47.40 ± 4.50 μM). Compound 4 exhibited significant cytotoxicity against all tested cell lines.

  • anti inflammatory steroidal glycosides from the berries of Solanum Nigrum l european black nightshade
    Phytochemistry, 2018
    Co-Authors: Limin Xiang, Yihai Wang
    Abstract:

    Abstract Seven previously undescribed steroidal glycosides, along with three known congeners were isolated from the unripe berries of Solanum Nigrum L. (Solanaceae). Their structures were elucidated on basis of 1D and 2D NMR, HR-ESI-MS spectroscopic data and GC analysis after acid hydrolysis. The potential inhibitory effects on nitric oxide (NO) production induced by lipopolysaccharide in RAW 264.7 cell line and the anti-proliferative activities against five cancer cell lines (HL-60, U-937, Jurkat, K562 and HepG2) were evaluated. Seven compounds exhibited inhibition activities on NO production with IC50 values ranging from 11.33 to 49.35 μM. Structure-activity relationships of the isolated compounds were also discussed.

  • potential anti inflammatory steroidal saponins from the berries of Solanum Nigrum l european black nightshade
    Journal of Agricultural and Food Chemistry, 2017
    Co-Authors: Yihai Wang, Limin Xiang
    Abstract:

    Solanum Nigrum L. or European black nightshade (Solanum genus) is a common weed of crops and gardens. The berries and leaves of S. Nigrum L. are consumed as food or vegetable in some regions and reported to possess a range of biological activities. In this study, nine new steroidal saponins, solanigrosides Y1–Y9 (1–6, 10–12), together with seven known congeners, were isolated from the berries of S. Nigrum. Their potential inhibitory effects on nitric oxide (NO) and IL-6 and IL-1β production induced by lipopolysaccharide (LPS) in macrophages cell line RAW 264.7 were evaluated. Compound 1 exhibited significant inhibition on NO production with an IC50 value of 9.7 μM, and some compounds exhibited significant inhibition effects on the LPS-induced IL-6 and IL-1β production. These results suggest that the steroidal saponins from berries of S. Nigrum demonstrated pronounced anti-inflammatory activity and might be explored as a healthy benefit agent.

Huiping Dai - One of the best experts on this subject based on the ideXlab platform.

Shenglian Luo - One of the best experts on this subject based on the ideXlab platform.

  • effect of endophyte infection on growth parameters and cd induced phytotoxicity of cd hyperaccumulator Solanum Nigrum l
    Chemosphere, 2012
    Co-Authors: Yong Wan, Jueliang Chen, Shenglian Luo, Xiao Xiao, Guangming Zeng, Liang Chen, Chengbin Liu
    Abstract:

    The aim of this work was to evaluate effects of endophytic bacterium inoculation on plant growth and assess the possible mechanism of endophyte in heavy metal phytoremediation. Seeds of Solanum Nigrum L. were inoculated with endophyte Serratia nematodiphila LRE07 and were subjected to Cd in the growing medium. Cd produced a significant inhibition on plant growth and a reduction in the content of photosynthetic pigments. The inoculation of endophytic bacterium alleviated the Cd-induced changes, resulting in more biomass production and higher photosynthetic pigments content of leaves compared with non-symbiotic ones. The beneficial effect was more obvious at relatively low Cd concentration (10 μM). Based on the alteration of nutrient uptake and activated oxygen metabolism in infected plants, the possible mechanisms of endophytic bacterium in Cd phytotoxicity reduction can be concluded as uptake enhancement of essential mineral nutrition and improvement in the antioxidative enzymes activities in infected plant.

  • Isolation and characterization of endophytic bacterium LRE07 from cadmium hyperaccumulator Solanum Nigrum L. and its potential for remediation
    Applied Microbiology and Biotechnology, 2011
    Co-Authors: Shenglian Luo, Hanjun Guo, Chengbin Liu, Qiang Xi, Yong Wan, Xiao Xiao, Guangming Zeng, Liang Chen, Jueliang Chen
    Abstract:

    Valuable endophytic strains facilitating plants growth and detoxification of heavy metals are required because the application of plant-endophyte symbiotic system is a promising potential technique to improve efficiency of phytoremediation. In this study, endophytic bacterium LRE07 was isolated from cadmium hyperaccumulator Solanum Nigrum L. It was identified as Serratia sp. by 16S rRNA sequence analysis. The endophytic bacterium LRE07 was resistant to the toxic effects of heavy metals, solubilized mineral phosphate, and produced indoleacetic acid and siderophore. The heavy metal detoxification was studied in growing LRE07 cells. The strain bound over 65% of cadmium and 35% of zinc in its growing cells from single metal solutions 72 h after inoculation. Besides the high removal efficiencies in single-ion system, an analogous removal phenomenon was also observed in multi-ions system, indicating that the endophyte possesses specific and remarkable heavy metal remediation abilities.

  • biosorption of cadmium by endophytic fungus ef microsphaeropsis sp lse10 isolated from cadmium hyperaccumulator Solanum Nigrum l
    Bioresource Technology, 2010
    Co-Authors: Xiao Xiao, Hanjun Guo, Shenglian Luo, Yong Wan, Guangming Zeng, Liang Chen, Wanzhi Wei, Zhe Cao, Lixia Yang
    Abstract:

    Abstract A novel technology to obtain highly efficient biosorbent from the endophytes of a hyperaccumulator is reported. This technology is more convenient than the traditional method of obtaining biosorbents by experimentally screening many types of biomass by trial and error. Using this technology, endophytic fungus (EF) LSE10 was isolated from the cadmium hyperaccumulator Solanum Nigrum L. It was identified as Microsphaeropsis sp. When cultured in vitro, the biomass yield of this EF was more than twice that of none-endophytic fungus (NEF) Rhizopus cohnii. Subsequently, it was used as a biosorbent for biosorption of cadmium from the aqueous solution. The results showed that the maximum biosorption capacity was 247.5 mg/g (2.2 mmol/g) which was much higher than those of other adsorbents, including biosorbents and activated carbon. Carboxyl, amino, sulphonate and hydroxyl groups on EF LSE10 surface were responsible for the biosorption of cadmium.