The Experts below are selected from a list of 141 Experts worldwide ranked by ideXlab platform
Wolfgang Dauber - One of the best experts on this subject based on the ideXlab platform.
-
About the morphological relationships of the sarcoplasmic reticulum in the Sole Plate area of the frog.
Tissue & Cell, 2004Co-Authors: Tilman Voigt, Wolfgang DauberAbstract:In the present investigation the Sole Plate area of motor end Plates of the frog is ultrastructurally examined with different postfixation methods. We concentrated in this case on the proof of the smooth and rough sarcoplasmic reticulum of the Sole Plate. The relations of the smooth and rough sarcoplasmic reticulum to subsynaptic folds and the local T-system and its connections to diads and triads in the Sole Plate area are represented. The morphological differences between mammal and frog are pointed out. The possible functions of the sarcoplasmic reticulum in the myofibril-free sarcoplasm are discussed.
-
Shape and position of the sarcoplasmic reticulum and the Golgi apparatus in the Sole Plate and remaining subsarcolemmal muscle region of the mouse using imidazole-osmium staining.
Microscopy Research and Technique, 2003Co-Authors: Tilman Voigt, Wolfgang Dauber, Irina Bensemann-ryvkin, Xenia HärtelAbstract:ABSTRACT By means of thin ( 150 nm) and thick ( 150 nm) sections, the shape and positionof the sarcoplasmic reticulum and of the Golgi apparatus in the Sole Plate and in the remainingsubsarcolemmal sarcoplasmic region were investigated. For this purpose the membranes werestained by means of imidazole-osmium postfixation and unstained sections analyzed under theelectron microscope. Both in the sarcoplasma of the Sole Plate and around the muscle fiber nuclei,a network of tubules is visible after imidazole-osmium staining which can be identified as thesarcoplasmic reticulum Solely on the basis of its contacts with the perinuclear cistern and thecisterns of the triads. Findings in literature on the position of the Golgi apparatus are confirmedand similar spatial relationships and vesiculations between the perinuclear cisterns and the Golgiapparatus of the Sole Plate nuclei and the other subsarcolemmal fiber nuclei are also demonstratedusing this new staining method. INTRODUCTION Earlier investigations of the motor end Plate (MEP)have shown that the transversal tubular system equalsthe T-system, which is responsible for the excitationpropagation into the interior of muscle fibers. Thissystemhasanet-likearchitectureintheSolePlatewithcontinuous connections to the subsynaptic folds (Daub-er et al., 1999, 2000). The sarcoplasmatic reticulum(SR) also occurs in the MEP in a rough and smoothform. Extensive information is available about theshape, extent, and molecular equipment and the pro-teins of the myofibrillar smooth SR of muscle fiberswhich run along the myofibrils and the subsarcolem-mal space outside the MEP (Volpe et al., 1992; Villa etal., 1993). However, there is a lack of correspondinginformation about the SR in the Sole Plate. In histolog-ical sections T-tubules and tubules of the smooth SRcannot be distinguished without the existence of spe-cial structure characteristics like triads, which are acombination of two SR cisterns with a central T-tu-bules (Dauber et al., 1999), or without the filling of theT-system with extracellular markers; for example, lan-thanum (Dauber et al., 2000).The aim of the present examination was to analyzemore exactly the shape of the SR in the Sole Plate. Forthis purpose Sole Plates of muscle fibers of mice dia-phragms were examined with the aid of imidazole-osmium postfixation, which is especially suitable forthe representation of membranes and, furthermore,makes the conventional staining of sections to be ex-amined unnecessary (Voigt et al., 2002). The SR wasidentified due to its connections with the perinuclearcistern and through the coformation of triads. In orderto raise the likelihood of a successful search, an exam-ination was carried out also on thick sections( 150 nm). Additionally, it turned out that the methodis suitable for visualizing the relation between theGolgi apparatus and the perinuclear cisterns, of boththe Sole Plate nuclei and the fiber nuclei outside of theMEP.
-
The T-tubular network and its triads in the Sole Plate sarcoplasm of the motor end-Plate of mammals
Journal of Muscle Research & Cell Motility, 2000Co-Authors: Wolfgang Dauber, Tilman Voigt, Xenia Härtel, Joachim MayerAbstract:The transition between the subsynaptic folds and the T-tubules demonstrated in a former paper was further investigated in the Sole Plate area by using the extracellular marker Lanthanum. A tubular network of the T-system of the Sole Plate area which is connected to the subsynaptic folds and to the t-tubular elements between the myofibrils is described for the first time. T-tubules of this network criss-cross through the sarcoplasm of the Sole Plate and lie in close proximity to Sole Plate nuclei and mitochondria. Cisterns of sarcoplasmic reticulum of the Sole Plate area make contact with these t-tubules forming triads. The possible physiological role of this Sole Plate network and its triads will be discussed with regard to a transport of substances in tubules with the dimension of nanotubes and Ca^2+ activated processes in the Sole Plate area.
-
Junctions between subsynaptic folds and rough sarcoplasmic reticulum of muscle fibres
Journal of Muscle Research & Cell Motility, 1999Co-Authors: Wolfgang Dauber, Tilman Voigt, Anne HeiniAbstract:Serial sections through motor end Plate regions of mouse muscle fibres demonstrated junctions between the subsynaptic folds and the rough sarcoplasmic reticulum of the Sole Plate nuclei. The shape of these structures resembles that of the well-known peripheral couplings, diads and triads of muscle fibres. However, the location of the new junctions between the surface membrane and the Sole Plate nuclei at a large distance from myofibrils, indicates a different function. The connection with the rough sarcoplasmic reticulum possibly influence the regulation of fibre protein metabolism, for example, gene expression for acetylcholine receptor synthesis.
Tilman Voigt - One of the best experts on this subject based on the ideXlab platform.
-
About the morphological relationships of the sarcoplasmic reticulum in the Sole Plate area of the frog.
Tissue & Cell, 2004Co-Authors: Tilman Voigt, Wolfgang DauberAbstract:In the present investigation the Sole Plate area of motor end Plates of the frog is ultrastructurally examined with different postfixation methods. We concentrated in this case on the proof of the smooth and rough sarcoplasmic reticulum of the Sole Plate. The relations of the smooth and rough sarcoplasmic reticulum to subsynaptic folds and the local T-system and its connections to diads and triads in the Sole Plate area are represented. The morphological differences between mammal and frog are pointed out. The possible functions of the sarcoplasmic reticulum in the myofibril-free sarcoplasm are discussed.
-
Shape and position of the sarcoplasmic reticulum and the Golgi apparatus in the Sole Plate and remaining subsarcolemmal muscle region of the mouse using imidazole-osmium staining.
Microscopy Research and Technique, 2003Co-Authors: Tilman Voigt, Wolfgang Dauber, Irina Bensemann-ryvkin, Xenia HärtelAbstract:ABSTRACT By means of thin ( 150 nm) and thick ( 150 nm) sections, the shape and positionof the sarcoplasmic reticulum and of the Golgi apparatus in the Sole Plate and in the remainingsubsarcolemmal sarcoplasmic region were investigated. For this purpose the membranes werestained by means of imidazole-osmium postfixation and unstained sections analyzed under theelectron microscope. Both in the sarcoplasma of the Sole Plate and around the muscle fiber nuclei,a network of tubules is visible after imidazole-osmium staining which can be identified as thesarcoplasmic reticulum Solely on the basis of its contacts with the perinuclear cistern and thecisterns of the triads. Findings in literature on the position of the Golgi apparatus are confirmedand similar spatial relationships and vesiculations between the perinuclear cisterns and the Golgiapparatus of the Sole Plate nuclei and the other subsarcolemmal fiber nuclei are also demonstratedusing this new staining method. INTRODUCTION Earlier investigations of the motor end Plate (MEP)have shown that the transversal tubular system equalsthe T-system, which is responsible for the excitationpropagation into the interior of muscle fibers. Thissystemhasanet-likearchitectureintheSolePlatewithcontinuous connections to the subsynaptic folds (Daub-er et al., 1999, 2000). The sarcoplasmatic reticulum(SR) also occurs in the MEP in a rough and smoothform. Extensive information is available about theshape, extent, and molecular equipment and the pro-teins of the myofibrillar smooth SR of muscle fiberswhich run along the myofibrils and the subsarcolem-mal space outside the MEP (Volpe et al., 1992; Villa etal., 1993). However, there is a lack of correspondinginformation about the SR in the Sole Plate. In histolog-ical sections T-tubules and tubules of the smooth SRcannot be distinguished without the existence of spe-cial structure characteristics like triads, which are acombination of two SR cisterns with a central T-tu-bules (Dauber et al., 1999), or without the filling of theT-system with extracellular markers; for example, lan-thanum (Dauber et al., 2000).The aim of the present examination was to analyzemore exactly the shape of the SR in the Sole Plate. Forthis purpose Sole Plates of muscle fibers of mice dia-phragms were examined with the aid of imidazole-osmium postfixation, which is especially suitable forthe representation of membranes and, furthermore,makes the conventional staining of sections to be ex-amined unnecessary (Voigt et al., 2002). The SR wasidentified due to its connections with the perinuclearcistern and through the coformation of triads. In orderto raise the likelihood of a successful search, an exam-ination was carried out also on thick sections( 150 nm). Additionally, it turned out that the methodis suitable for visualizing the relation between theGolgi apparatus and the perinuclear cisterns, of boththe Sole Plate nuclei and the fiber nuclei outside of theMEP.
-
The T-tubular network and its triads in the Sole Plate sarcoplasm of the motor end-Plate of mammals
Journal of Muscle Research & Cell Motility, 2000Co-Authors: Wolfgang Dauber, Tilman Voigt, Xenia Härtel, Joachim MayerAbstract:The transition between the subsynaptic folds and the T-tubules demonstrated in a former paper was further investigated in the Sole Plate area by using the extracellular marker Lanthanum. A tubular network of the T-system of the Sole Plate area which is connected to the subsynaptic folds and to the t-tubular elements between the myofibrils is described for the first time. T-tubules of this network criss-cross through the sarcoplasm of the Sole Plate and lie in close proximity to Sole Plate nuclei and mitochondria. Cisterns of sarcoplasmic reticulum of the Sole Plate area make contact with these t-tubules forming triads. The possible physiological role of this Sole Plate network and its triads will be discussed with regard to a transport of substances in tubules with the dimension of nanotubes and Ca^2+ activated processes in the Sole Plate area.
-
Junctions between subsynaptic folds and rough sarcoplasmic reticulum of muscle fibres
Journal of Muscle Research & Cell Motility, 1999Co-Authors: Wolfgang Dauber, Tilman Voigt, Anne HeiniAbstract:Serial sections through motor end Plate regions of mouse muscle fibres demonstrated junctions between the subsynaptic folds and the rough sarcoplasmic reticulum of the Sole Plate nuclei. The shape of these structures resembles that of the well-known peripheral couplings, diads and triads of muscle fibres. However, the location of the new junctions between the surface membrane and the Sole Plate nuclei at a large distance from myofibrils, indicates a different function. The connection with the rough sarcoplasmic reticulum possibly influence the regulation of fibre protein metabolism, for example, gene expression for acetylcholine receptor synthesis.
Anne Heini - One of the best experts on this subject based on the ideXlab platform.
-
Junctions between subsynaptic folds and rough sarcoplasmic reticulum of muscle fibres
Journal of Muscle Research & Cell Motility, 1999Co-Authors: Wolfgang Dauber, Tilman Voigt, Anne HeiniAbstract:Serial sections through motor end Plate regions of mouse muscle fibres demonstrated junctions between the subsynaptic folds and the rough sarcoplasmic reticulum of the Sole Plate nuclei. The shape of these structures resembles that of the well-known peripheral couplings, diads and triads of muscle fibres. However, the location of the new junctions between the surface membrane and the Sole Plate nuclei at a large distance from myofibrils, indicates a different function. The connection with the rough sarcoplasmic reticulum possibly influence the regulation of fibre protein metabolism, for example, gene expression for acetylcholine receptor synthesis.
Xenia Härtel - One of the best experts on this subject based on the ideXlab platform.
-
Shape and position of the sarcoplasmic reticulum and the Golgi apparatus in the Sole Plate and remaining subsarcolemmal muscle region of the mouse using imidazole-osmium staining.
Microscopy Research and Technique, 2003Co-Authors: Tilman Voigt, Wolfgang Dauber, Irina Bensemann-ryvkin, Xenia HärtelAbstract:ABSTRACT By means of thin ( 150 nm) and thick ( 150 nm) sections, the shape and positionof the sarcoplasmic reticulum and of the Golgi apparatus in the Sole Plate and in the remainingsubsarcolemmal sarcoplasmic region were investigated. For this purpose the membranes werestained by means of imidazole-osmium postfixation and unstained sections analyzed under theelectron microscope. Both in the sarcoplasma of the Sole Plate and around the muscle fiber nuclei,a network of tubules is visible after imidazole-osmium staining which can be identified as thesarcoplasmic reticulum Solely on the basis of its contacts with the perinuclear cistern and thecisterns of the triads. Findings in literature on the position of the Golgi apparatus are confirmedand similar spatial relationships and vesiculations between the perinuclear cisterns and the Golgiapparatus of the Sole Plate nuclei and the other subsarcolemmal fiber nuclei are also demonstratedusing this new staining method. INTRODUCTION Earlier investigations of the motor end Plate (MEP)have shown that the transversal tubular system equalsthe T-system, which is responsible for the excitationpropagation into the interior of muscle fibers. Thissystemhasanet-likearchitectureintheSolePlatewithcontinuous connections to the subsynaptic folds (Daub-er et al., 1999, 2000). The sarcoplasmatic reticulum(SR) also occurs in the MEP in a rough and smoothform. Extensive information is available about theshape, extent, and molecular equipment and the pro-teins of the myofibrillar smooth SR of muscle fiberswhich run along the myofibrils and the subsarcolem-mal space outside the MEP (Volpe et al., 1992; Villa etal., 1993). However, there is a lack of correspondinginformation about the SR in the Sole Plate. In histolog-ical sections T-tubules and tubules of the smooth SRcannot be distinguished without the existence of spe-cial structure characteristics like triads, which are acombination of two SR cisterns with a central T-tu-bules (Dauber et al., 1999), or without the filling of theT-system with extracellular markers; for example, lan-thanum (Dauber et al., 2000).The aim of the present examination was to analyzemore exactly the shape of the SR in the Sole Plate. Forthis purpose Sole Plates of muscle fibers of mice dia-phragms were examined with the aid of imidazole-osmium postfixation, which is especially suitable forthe representation of membranes and, furthermore,makes the conventional staining of sections to be ex-amined unnecessary (Voigt et al., 2002). The SR wasidentified due to its connections with the perinuclearcistern and through the coformation of triads. In orderto raise the likelihood of a successful search, an exam-ination was carried out also on thick sections( 150 nm). Additionally, it turned out that the methodis suitable for visualizing the relation between theGolgi apparatus and the perinuclear cisterns, of boththe Sole Plate nuclei and the fiber nuclei outside of theMEP.
-
The T-tubular network and its triads in the Sole Plate sarcoplasm of the motor end-Plate of mammals
Journal of Muscle Research & Cell Motility, 2000Co-Authors: Wolfgang Dauber, Tilman Voigt, Xenia Härtel, Joachim MayerAbstract:The transition between the subsynaptic folds and the T-tubules demonstrated in a former paper was further investigated in the Sole Plate area by using the extracellular marker Lanthanum. A tubular network of the T-system of the Sole Plate area which is connected to the subsynaptic folds and to the t-tubular elements between the myofibrils is described for the first time. T-tubules of this network criss-cross through the sarcoplasm of the Sole Plate and lie in close proximity to Sole Plate nuclei and mitochondria. Cisterns of sarcoplasmic reticulum of the Sole Plate area make contact with these t-tubules forming triads. The possible physiological role of this Sole Plate network and its triads will be discussed with regard to a transport of substances in tubules with the dimension of nanotubes and Ca^2+ activated processes in the Sole Plate area.
Joachim Mayer - One of the best experts on this subject based on the ideXlab platform.
-
The T-tubular network and its triads in the Sole Plate sarcoplasm of the motor end-Plate of mammals
Journal of Muscle Research & Cell Motility, 2000Co-Authors: Wolfgang Dauber, Tilman Voigt, Xenia Härtel, Joachim MayerAbstract:The transition between the subsynaptic folds and the T-tubules demonstrated in a former paper was further investigated in the Sole Plate area by using the extracellular marker Lanthanum. A tubular network of the T-system of the Sole Plate area which is connected to the subsynaptic folds and to the t-tubular elements between the myofibrils is described for the first time. T-tubules of this network criss-cross through the sarcoplasm of the Sole Plate and lie in close proximity to Sole Plate nuclei and mitochondria. Cisterns of sarcoplasmic reticulum of the Sole Plate area make contact with these t-tubules forming triads. The possible physiological role of this Sole Plate network and its triads will be discussed with regard to a transport of substances in tubules with the dimension of nanotubes and Ca^2+ activated processes in the Sole Plate area.