The Experts below are selected from a list of 57 Experts worldwide ranked by ideXlab platform
S. N. Bhatia - One of the best experts on this subject based on the ideXlab platform.
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Micromechanical control of cell–cell interactions
Proceedings of the National Academy of Sciences of the United States of America, 2007Co-Authors: S. N. BhatiaAbstract:The development and function of living tissues depends largely on interactions between cells that can vary in both time and space; however, temporal control of cell–cell interaction is experimentally challenging. By using a micromachined silicon substrate with moving parts, we demonstrate the dynamic regulation of cell–cell interactions via direct manipulation of adherent cells with micrometer-scale precision. We thereby achieve mechanical control of both tissue composition and spatial organization. As a case study, we demonstrate the utility of this tool in deconstructing the dynamics of intercellular communication between hepatocytes and supportive stromal cells in coculture. Our findings indicate that the maintenance of the hepatocellular phenotype by stroma requires direct contact for a limited time (≈hours) followed by a sustained Soluble Signal that has an effective range of
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Micromechanical control of cell-cell interactions
Proceedings of the National Academy of Sciences, 2007Co-Authors: E. E. Hui, S. N. BhatiaAbstract:The development and function of living tissues depends largely on interactions between cells that can vary in both time and space; however, temporal control of cell-cell interaction is experimentally challenging. By using a micromachined silicon substrate with moving parts, we demonstrate the dynamic regulation of cell-cell interactions via direct manipulation of adherent cells with micrometer-scale precision. We thereby achieve mechanical control of both tissue composition and spatial organization. As a case study, we demonstrate the utility of this tool in deconstructing the dynamics of intercellular communication between hepatocytes and supportive stromal cells in coculture. Our findings indicate that the maintenance of the hepatocellular phenotype by stroma requires direct contact for a limited time ( approximately hours) followed by a sustained Soluble Signal that has an effective range of
Chang You Gao - One of the best experts on this subject based on the ideXlab platform.
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Gradient biomaterials and their influences on cell migration
Interface Focus, 2012Co-Authors: Jindan Wu, Tanchen Ren, Huaping Tan, Lulu Han, Zhengwei Mao, Chang You GaoAbstract:Cell migration participates in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. The cells specifically migrate to destiny sites induced by the gradually varying concentration (gradient) of Soluble Signal factors and the ligands bound with the extracellular matrix in the body during a wound healing process. Therefore, regulation of the cell migration behaviours is of paramount importance in regenerative medicine. One important way is to create a microenvironment that mimics the in vivo cellular and tissue complexity by incorporating physical, chemical and biological Signal gradients into engineered biomaterials. In this review, the gradients existing in vivo and their influences on cell migration are briefly described. Recent developments in the fabrication of gradient biomaterials for controlling cellular behaviours, especially the cell migration, are summarized, highlighting the importance of the intrinsic driving mechanism for tissue regeneration and the design principle of complicated and advanced tissue regenerative materials. The potential uses of the gradient biomaterials in regenerative medicine are introduced. The current and future trends in gradient biomaterials and programmed cell migration in terms of the long-term goals of tissue regeneration are prospected.
Erling Sundrehagen - One of the best experts on this subject based on the ideXlab platform.
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Soluble highly coloured phenylboronic acids and their use in glycohemoglobin quantification
Clinica Chimica Acta, 1997Co-Authors: Frank Frantzen, Dagerik Heggli, Kjersti Grimsrud, Erling SundrehagenAbstract:Water Soluble dye-phenylboronic acid conjugates (dye-PBAs) possessing strong absorption of visible light are introduced as new reagents for the determination of glycohemoglobin. Their functionality and prospective use are demonstrated in a semi-homogenous glycohemoglobin assay. The assay is based on cis-diol esterification of dye-PBA to glycohemoglobin followed by selective precipitation of hemoglobin from solution, co-precipitating bound dye-PBA. Quantification of the molar “dye-PBA/Hb”-ratio in redissolved precipitates using either absorption or fluorescence spectroscopy, reflects the glycation level of the blood samples used. Future development of the assay principle is illustrated in a filter based assay, collecting the precipitated hemoglobin on a filter followed by reflectometric readings directly on the precipitate. The significance of this work lies first, in the demonstration of a new principle for the determination of glycohemoglobin, and second, as an illustration of the prospective use of water Soluble, Signal-forming non-immobilised boronic acids in the determination of cis-diol containing analytes.
Jindan Wu - One of the best experts on this subject based on the ideXlab platform.
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Gradient biomaterials and their influences on cell migration
Interface Focus, 2012Co-Authors: Jindan Wu, Tanchen Ren, Huaping Tan, Lulu Han, Zhengwei Mao, Chang You GaoAbstract:Cell migration participates in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. The cells specifically migrate to destiny sites induced by the gradually varying concentration (gradient) of Soluble Signal factors and the ligands bound with the extracellular matrix in the body during a wound healing process. Therefore, regulation of the cell migration behaviours is of paramount importance in regenerative medicine. One important way is to create a microenvironment that mimics the in vivo cellular and tissue complexity by incorporating physical, chemical and biological Signal gradients into engineered biomaterials. In this review, the gradients existing in vivo and their influences on cell migration are briefly described. Recent developments in the fabrication of gradient biomaterials for controlling cellular behaviours, especially the cell migration, are summarized, highlighting the importance of the intrinsic driving mechanism for tissue regeneration and the design principle of complicated and advanced tissue regenerative materials. The potential uses of the gradient biomaterials in regenerative medicine are introduced. The current and future trends in gradient biomaterials and programmed cell migration in terms of the long-term goals of tissue regeneration are prospected.
P. Spangenberg - One of the best experts on this subject based on the ideXlab platform.
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Activation of leukocytes in whole blood samples by N-formyl-methionyl-leucyl-phenylalanine (FMLP) enhances platelet aggregability but not platelet P-selectin exposure and adhesion to leukocytes
Platelets, 1998Co-Authors: Wolfgang Lösche, H. Redlich, Krause S, Stan Heptinstall, P. SpangenbergAbstract:Adhesion of platelets to neutrophils and monocytes is believed to play an important role in intercellular communication. Evidence has been provided that such heterotypic cell-cell contacts via adhesion molecules may be directly involved in intercellular Signal transduction as well as facilitate the action of Soluble Signal transmitters, e.g. cathepsin G, PAF or nitric oxide. With respect to platelet activation, stimulatory and inhibitory effects of leukocytes have been reported, and the results obtained seem to be influenced by the experimental conditions. We investigated the effect of leukocyte stimulation on platelet behaviour in samples of human citrated whole blood. Adding the chemotactic peptide FM LP, which stimulates neutrophils and monocytes but not lymphocytes and platelets, to stirred whole blood samples resulted in a significant enhancement ( P < 0.01) of spontaneous as well as ADP-induced platelet aggregation (25 vs 33% and 66 vs 69%, respectively). In contrast stirring-induced as well as A...