The Experts below are selected from a list of 291 Experts worldwide ranked by ideXlab platform
C. L. Encina - One of the best experts on this subject based on the ideXlab platform.
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Study of the Somaclonal Variation produced by different methods of polyploidization in Asparagus officinalis L.
Plant Cell Tissue and Organ Culture (PCTOC), 2015Co-Authors: J. J. Regalado, E. Carmona Martín, P. Castro, R. Moreno, C. L. EncinaAbstract:Polyploid plants have been induced in different Asparagus officinalis L. breeding programs in order to obtain plants with improved agronomical traits, such as large spear diameter or segregation ratios with a higher number of males. The polyploidization methods can produce Somaclonal Variation in the polyploid plants obtained and, therefore, unwanted changes in the agronomical traits of the initial elite plants. We used two different polyploidization methods to induce polyploid plants from diploid genotypes of commercial varieties and tetraploid genotypes of the Spanish landrace “Morado de Huétor”. The first method was the culture of rhizome buds in the medium ARBM-3 (Asparagus Rhizome Bud Medium), supplemented with different concentrations of colchicine (0.1–0.75 g l^−1) for 10 and 20 days. The best polyploidization rate obtained was 25 % (0.5 g l^−1 colchicine for 10 days). The second method was the regeneration of polyploid plants from callus culture, resulting in a polyploidization rate of 40 and 12.5 % for the diploid genotype CM077 and the tetraploid genotype HT156, respectively. Additionally, we have developed a new protocol to separate the mixoploids generated into their different genetic components, obtaining plants with a unique ploidy level. EST-SSRs markers were employed to analyze the genetic stability of polyploidy plants. Somaclonal Variation was not detected for polyploidy plants obtained through the culture of rhizome bud explants. Therefore, these polyploid plants should maintain the agronomical traits of the initial elite plants. However, Somaclonal Variation was detected in the polyploid plants regenerated from callus culture.
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study of the Somaclonal Variation produced by different methods of polyploidization in asparagus officinalis l
Plant Cell Tissue and Organ Culture, 2015Co-Authors: J. J. Regalado, P. Castro, R. Moreno, Carmona E Martin, J Gil, C. L. EncinaAbstract:Polyploid plants have been induced in different Asparagus officinalis L. breeding programs in order to obtain plants with improved agronomical traits, such as large spear diameter or segregation ratios with a higher number of males. The polyploidization methods can produce Somaclonal Variation in the polyploid plants obtained and, therefore, unwanted changes in the agronomical traits of the initial elite plants. We used two different polyploidization methods to induce polyploid plants from diploid genotypes of commercial varieties and tetraploid genotypes of the Spanish landrace “Morado de Huetor”. The first method was the culture of rhizome buds in the medium ARBM-3 (Asparagus Rhizome Bud Medium), supplemented with different concentrations of colchicine (0.1–0.75 g l−1) for 10 and 20 days. The best polyploidization rate obtained was 25 % (0.5 g l−1 colchicine for 10 days). The second method was the regeneration of polyploid plants from callus culture, resulting in a polyploidization rate of 40 and 12.5 % for the diploid genotype CM077 and the tetraploid genotype HT156, respectively. Additionally, we have developed a new protocol to separate the mixoploids generated into their different genetic components, obtaining plants with a unique ploidy level. EST-SSRs markers were employed to analyze the genetic stability of polyploidy plants. Somaclonal Variation was not detected for polyploidy plants obtained through the culture of rhizome bud explants. Therefore, these polyploid plants should maintain the agronomical traits of the initial elite plants. However, Somaclonal Variation was detected in the polyploid plants regenerated from callus culture.
J. J. Regalado - One of the best experts on this subject based on the ideXlab platform.
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Study of the Somaclonal Variation produced by different methods of polyploidization in Asparagus officinalis L.
Plant Cell Tissue and Organ Culture (PCTOC), 2015Co-Authors: J. J. Regalado, E. Carmona Martín, P. Castro, R. Moreno, C. L. EncinaAbstract:Polyploid plants have been induced in different Asparagus officinalis L. breeding programs in order to obtain plants with improved agronomical traits, such as large spear diameter or segregation ratios with a higher number of males. The polyploidization methods can produce Somaclonal Variation in the polyploid plants obtained and, therefore, unwanted changes in the agronomical traits of the initial elite plants. We used two different polyploidization methods to induce polyploid plants from diploid genotypes of commercial varieties and tetraploid genotypes of the Spanish landrace “Morado de Huétor”. The first method was the culture of rhizome buds in the medium ARBM-3 (Asparagus Rhizome Bud Medium), supplemented with different concentrations of colchicine (0.1–0.75 g l^−1) for 10 and 20 days. The best polyploidization rate obtained was 25 % (0.5 g l^−1 colchicine for 10 days). The second method was the regeneration of polyploid plants from callus culture, resulting in a polyploidization rate of 40 and 12.5 % for the diploid genotype CM077 and the tetraploid genotype HT156, respectively. Additionally, we have developed a new protocol to separate the mixoploids generated into their different genetic components, obtaining plants with a unique ploidy level. EST-SSRs markers were employed to analyze the genetic stability of polyploidy plants. Somaclonal Variation was not detected for polyploidy plants obtained through the culture of rhizome bud explants. Therefore, these polyploid plants should maintain the agronomical traits of the initial elite plants. However, Somaclonal Variation was detected in the polyploid plants regenerated from callus culture.
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study of the Somaclonal Variation produced by different methods of polyploidization in asparagus officinalis l
Plant Cell Tissue and Organ Culture, 2015Co-Authors: J. J. Regalado, P. Castro, R. Moreno, Carmona E Martin, J Gil, C. L. EncinaAbstract:Polyploid plants have been induced in different Asparagus officinalis L. breeding programs in order to obtain plants with improved agronomical traits, such as large spear diameter or segregation ratios with a higher number of males. The polyploidization methods can produce Somaclonal Variation in the polyploid plants obtained and, therefore, unwanted changes in the agronomical traits of the initial elite plants. We used two different polyploidization methods to induce polyploid plants from diploid genotypes of commercial varieties and tetraploid genotypes of the Spanish landrace “Morado de Huetor”. The first method was the culture of rhizome buds in the medium ARBM-3 (Asparagus Rhizome Bud Medium), supplemented with different concentrations of colchicine (0.1–0.75 g l−1) for 10 and 20 days. The best polyploidization rate obtained was 25 % (0.5 g l−1 colchicine for 10 days). The second method was the regeneration of polyploid plants from callus culture, resulting in a polyploidization rate of 40 and 12.5 % for the diploid genotype CM077 and the tetraploid genotype HT156, respectively. Additionally, we have developed a new protocol to separate the mixoploids generated into their different genetic components, obtaining plants with a unique ploidy level. EST-SSRs markers were employed to analyze the genetic stability of polyploidy plants. Somaclonal Variation was not detected for polyploidy plants obtained through the culture of rhizome bud explants. Therefore, these polyploid plants should maintain the agronomical traits of the initial elite plants. However, Somaclonal Variation was detected in the polyploid plants regenerated from callus culture.
R. Moreno - One of the best experts on this subject based on the ideXlab platform.
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Study of the Somaclonal Variation produced by different methods of polyploidization in Asparagus officinalis L.
Plant Cell Tissue and Organ Culture (PCTOC), 2015Co-Authors: J. J. Regalado, E. Carmona Martín, P. Castro, R. Moreno, C. L. EncinaAbstract:Polyploid plants have been induced in different Asparagus officinalis L. breeding programs in order to obtain plants with improved agronomical traits, such as large spear diameter or segregation ratios with a higher number of males. The polyploidization methods can produce Somaclonal Variation in the polyploid plants obtained and, therefore, unwanted changes in the agronomical traits of the initial elite plants. We used two different polyploidization methods to induce polyploid plants from diploid genotypes of commercial varieties and tetraploid genotypes of the Spanish landrace “Morado de Huétor”. The first method was the culture of rhizome buds in the medium ARBM-3 (Asparagus Rhizome Bud Medium), supplemented with different concentrations of colchicine (0.1–0.75 g l^−1) for 10 and 20 days. The best polyploidization rate obtained was 25 % (0.5 g l^−1 colchicine for 10 days). The second method was the regeneration of polyploid plants from callus culture, resulting in a polyploidization rate of 40 and 12.5 % for the diploid genotype CM077 and the tetraploid genotype HT156, respectively. Additionally, we have developed a new protocol to separate the mixoploids generated into their different genetic components, obtaining plants with a unique ploidy level. EST-SSRs markers were employed to analyze the genetic stability of polyploidy plants. Somaclonal Variation was not detected for polyploidy plants obtained through the culture of rhizome bud explants. Therefore, these polyploid plants should maintain the agronomical traits of the initial elite plants. However, Somaclonal Variation was detected in the polyploid plants regenerated from callus culture.
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study of the Somaclonal Variation produced by different methods of polyploidization in asparagus officinalis l
Plant Cell Tissue and Organ Culture, 2015Co-Authors: J. J. Regalado, P. Castro, R. Moreno, Carmona E Martin, J Gil, C. L. EncinaAbstract:Polyploid plants have been induced in different Asparagus officinalis L. breeding programs in order to obtain plants with improved agronomical traits, such as large spear diameter or segregation ratios with a higher number of males. The polyploidization methods can produce Somaclonal Variation in the polyploid plants obtained and, therefore, unwanted changes in the agronomical traits of the initial elite plants. We used two different polyploidization methods to induce polyploid plants from diploid genotypes of commercial varieties and tetraploid genotypes of the Spanish landrace “Morado de Huetor”. The first method was the culture of rhizome buds in the medium ARBM-3 (Asparagus Rhizome Bud Medium), supplemented with different concentrations of colchicine (0.1–0.75 g l−1) for 10 and 20 days. The best polyploidization rate obtained was 25 % (0.5 g l−1 colchicine for 10 days). The second method was the regeneration of polyploid plants from callus culture, resulting in a polyploidization rate of 40 and 12.5 % for the diploid genotype CM077 and the tetraploid genotype HT156, respectively. Additionally, we have developed a new protocol to separate the mixoploids generated into their different genetic components, obtaining plants with a unique ploidy level. EST-SSRs markers were employed to analyze the genetic stability of polyploidy plants. Somaclonal Variation was not detected for polyploidy plants obtained through the culture of rhizome bud explants. Therefore, these polyploid plants should maintain the agronomical traits of the initial elite plants. However, Somaclonal Variation was detected in the polyploid plants regenerated from callus culture.
P. Castro - One of the best experts on this subject based on the ideXlab platform.
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Study of the Somaclonal Variation produced by different methods of polyploidization in Asparagus officinalis L.
Plant Cell Tissue and Organ Culture (PCTOC), 2015Co-Authors: J. J. Regalado, E. Carmona Martín, P. Castro, R. Moreno, C. L. EncinaAbstract:Polyploid plants have been induced in different Asparagus officinalis L. breeding programs in order to obtain plants with improved agronomical traits, such as large spear diameter or segregation ratios with a higher number of males. The polyploidization methods can produce Somaclonal Variation in the polyploid plants obtained and, therefore, unwanted changes in the agronomical traits of the initial elite plants. We used two different polyploidization methods to induce polyploid plants from diploid genotypes of commercial varieties and tetraploid genotypes of the Spanish landrace “Morado de Huétor”. The first method was the culture of rhizome buds in the medium ARBM-3 (Asparagus Rhizome Bud Medium), supplemented with different concentrations of colchicine (0.1–0.75 g l^−1) for 10 and 20 days. The best polyploidization rate obtained was 25 % (0.5 g l^−1 colchicine for 10 days). The second method was the regeneration of polyploid plants from callus culture, resulting in a polyploidization rate of 40 and 12.5 % for the diploid genotype CM077 and the tetraploid genotype HT156, respectively. Additionally, we have developed a new protocol to separate the mixoploids generated into their different genetic components, obtaining plants with a unique ploidy level. EST-SSRs markers were employed to analyze the genetic stability of polyploidy plants. Somaclonal Variation was not detected for polyploidy plants obtained through the culture of rhizome bud explants. Therefore, these polyploid plants should maintain the agronomical traits of the initial elite plants. However, Somaclonal Variation was detected in the polyploid plants regenerated from callus culture.
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study of the Somaclonal Variation produced by different methods of polyploidization in asparagus officinalis l
Plant Cell Tissue and Organ Culture, 2015Co-Authors: J. J. Regalado, P. Castro, R. Moreno, Carmona E Martin, J Gil, C. L. EncinaAbstract:Polyploid plants have been induced in different Asparagus officinalis L. breeding programs in order to obtain plants with improved agronomical traits, such as large spear diameter or segregation ratios with a higher number of males. The polyploidization methods can produce Somaclonal Variation in the polyploid plants obtained and, therefore, unwanted changes in the agronomical traits of the initial elite plants. We used two different polyploidization methods to induce polyploid plants from diploid genotypes of commercial varieties and tetraploid genotypes of the Spanish landrace “Morado de Huetor”. The first method was the culture of rhizome buds in the medium ARBM-3 (Asparagus Rhizome Bud Medium), supplemented with different concentrations of colchicine (0.1–0.75 g l−1) for 10 and 20 days. The best polyploidization rate obtained was 25 % (0.5 g l−1 colchicine for 10 days). The second method was the regeneration of polyploid plants from callus culture, resulting in a polyploidization rate of 40 and 12.5 % for the diploid genotype CM077 and the tetraploid genotype HT156, respectively. Additionally, we have developed a new protocol to separate the mixoploids generated into their different genetic components, obtaining plants with a unique ploidy level. EST-SSRs markers were employed to analyze the genetic stability of polyploidy plants. Somaclonal Variation was not detected for polyploidy plants obtained through the culture of rhizome bud explants. Therefore, these polyploid plants should maintain the agronomical traits of the initial elite plants. However, Somaclonal Variation was detected in the polyploid plants regenerated from callus culture.
A. M. Vázquez - One of the best experts on this subject based on the ideXlab platform.
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Insight into Somaclonal Variation
Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology, 2001Co-Authors: A. M. VázquezAbstract:ABSTRACT Somaclonal Variation (S.V.) refers to mutational events occurring in tissue culture, although some permanent methylation processes should possibly also be included under this name. In this review, the possible causes as well as the mechanisms implicated in the induction of mutation in cultured cells are discussed. The needs for an easy assay to assess S.V. is pointed out.
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Somaclonal Variation in rye
Mutation research, 1993Co-Authors: Rosario Linacero, A. M. VázquezAbstract:We studied the genetic Variation generated during in vitro culture of rye Secale cereale L. We analyzed the progenies of four generations of the plants regenerated from immature embryo cultures. A high frequency of mutant plants was observed, 50.75%, this frequency was genotype dependent. Other characteristics typical of Somaclonal Variation were also observed: the obtaining of dominant mutations, the presence of more than one mutation per plant, the obtaining of homozygous mutants and a high rate of mutation of particular loci. In some cases transposable elements could be implicated. We postulate that tissue culture could induce mutations as well as select particular cell types and so increase the appearance of special mutants.
