The Experts below are selected from a list of 231 Experts worldwide ranked by ideXlab platform

Martina Oberhofer - One of the best experts on this subject based on the ideXlab platform.

  • horizontal transmission persistence and competition capabilities of epichloe endophytes in hordelymus europaeus grass hosts using dual endophyte inocula
    Fungal Ecology, 2014
    Co-Authors: Martina Oberhofer, Adrian Leuchtmann
    Abstract:

    The Epichloae (Ascomycota, Clavicipitaceae) are endophytes of grasses consisting of haploid species, and heteroploid interspecific hybrids that are prevalent in many natural host grass populations. Hybrid formation may involve Somatic Fusion of different mycelia in planta, but this has never been tested. We used Hordelymus europaeus as an experimental system, which naturally hosts several hybrid and non-hybrid endophytes. Seedlings were inoculated with mixed inocula including a hybrid and a non-hybrid strain from H. europaeus, and two sexual Epichloe species from other hosts. Evaluation of endophyte infection 13 months after inoculation detected a high rate of multiple infections within tillers, but no novel hybrid endophyte. The hybrid strain was also found in plants that had not received such inoculum suggesting horizontal transmission. Epiphytic conidia detected on leaf tissues were responsible for contagious spread. This observation may offer an explanation why hybrid endophytes are prevalent in natural populations of H. europaeus.

  • horizontal transmission persistence and competition capabilities of epichloe endophytes in hordelymus europaeus grass hosts using dual endophyte inocula
    Fungal Ecology, 2014
    Co-Authors: Martina Oberhofer, Adrian Leuchtmann
    Abstract:

    The Epichloae (Ascomycota, Clavicipitaceae) are endophytes of grasses consisting of haploid species, and heteroploid interspecific hybrids that are prevalent in many natural host grass populations. Hybrid formation may involve Somatic Fusion of different mycelia in planta, but this has never been tested. We used Hordelymus europaeus as an experimental system, which naturally hosts several hybrid and non-hybrid endophytes. Seedlings were inoculated with mixed inocula including a hybrid and a non-hybrid strain from H. europaeus, and two sexual Epichloe species from other hosts. Evaluation of endophyte infection 13 months after inoculation detected a high rate of multiple infections within tillers, but no novel hybrid endophyte. The hybrid strain was also found in plants that had not received such inoculum suggesting horizontal transmission. Epiphytic conidia detected on leaf tissues were responsible for contagious spread. This observation may offer an explanation why hybrid endophytes are prevalent in natural populations of H. europaeus.

Garriga Calderé F. - One of the best experts on this subject based on the ideXlab platform.

  • Prospects for introgressing tomato chromosomes into the potato genome: An assessment through GISH analysis
    1999
    Co-Authors: Garriga Calderé F., Huigen D.j., Jacobsen E., Ramanna M.s.
    Abstract:

    With a view to assess the possibility of homoeologous pairing and crossing-over between the chromosomes of potato (Solanum tuberosum) and tomato (Lycopersicon esculentum), a Somatic Fusion hybrid and two monosomic alien tomato addition genotypes were investigated through genomic in situ hybridisation (GISH). The Somatic Fusion hybrid, C31-17-51, was near hexaploid (2n = 6x - 4 = 68) possessing 46 potato chromosomes + 20 tomato chromosomes + 2 translocated chromosomes. The two alien addition genotypes were near tetraploids (2n = 4x + 1 = 49) and consisted of monosomic alien additions for tomato chromosome 1 in genotype 2103-1, and tomato chromosome 8 in genotype 2301-2. In the Fusion hybrid the tomato pachytene chromosome identification revealed that the chromosomes 1, 2, 5, 6, 7, 10, and 12 were in diploid condition whereas among those that were in haploid condition, three could be identified viz., 4, 9, and 11. The remaining three chromosomes could not be cytologically identified. Although the chromosomes with translocated segments could not be identified at the pachytene stage due to technical difficulties, there was clear evidence for the presence of a reciprocal translocation observed at diakinesis and metaphase I stages. Because of autosyndetic pairing of the translocated segments, it gave a false impression as if there was a high frequency (86.0%) of allosyndetic pairing. In contrast to the Fusion hybrid, the two alien monosomic addition genotypes showed a very low frequency of allosyndetic pairing, namely 1.1 and 1.3% respectively for the monosomic additions 1 and 8. In the genotype 2301-2, monosomic addition for tomato chromosome 8, crossing-over between the homoeologous chromosomes was estimated to occur in 0.8% of the meiotic cells investigated. Despite this low frequency of homoeologous pairing and crossing-over, there is a possibility for introgressing tomato chromosomal DNA into the potato genome through intergenomic recombination

  • Introgression of tomato chromosomes into the potato genome : an analysis through molecular marker and in situ hybridisation techniques = [Introgressie van tomatenchromosomen in het aardappelgenoom : een analyse met behulp van moleculaire merker en in
    Garriga Caldere, 1998
    Co-Authors: Garriga Calderé F.
    Abstract:

    Transfer of alien chromosomes and genes across intergeneric boundaries can be useful not only for the introgression of desirable characters but also for fundamental genetic studies. The successful demonstration of hybridisation of potato ( Solanum tuberosum ) and tomato ( Lycopersicon esculentum ) through protoplast Fusion in 1978, created the potential for introgressing chromosomes and genes from one genus into the other. However, real prospects of adding tomato chromosomes into the potato genome arose only after a potato (+) tomato Fusion hybrid was successfully backcrossed to tetraploid potato in 1994.Despite this achievement, the problem of selective alien tomato chromosome elimination from the Fusion hybrid and the backcross progenies, as well as the behaviour of the alien tomato chromosomes in the potato background, raised the following questions: (i) in view of the selective elimination of alien tomato chromosomes, is it possible to establish a complete series of alien tomato chromosome additions in a potato background? (ii) do the introgressed alien chromosomes retain their structural integrity? (iii) can all the alien tomato chromosomes be transmitted through the gametes to the sexual progeny and if so, in what frequency? (iv) if there are any structural changes in the introgressed alien chromosomes, can they be identified and characterised? (v) do the potato and tomato chromosomes pair homoeologously and cross-over?An attempt was made in the present investigation to create the appropriate plant material to answer the five above mentioned questions. In this connection, the chromosome constitution of the Somatic Fusion hybrids and their backcross derivatives, viz., BC 1 , BC 2 and BC 3 progenies were analysed through a combination of restriction fragment length polymorphism (RFLP) using tomato chromosome specific probes, genomic in situ hybridisation (GISH) and fluorescence in situ hybridisation (FISH) analyses.In order to produce BC 1 progenies with the full potential for the creation of all the 12 possible monosomic tomato chromosome additions in a tetraploid potato background, three different hexaploid Somatic Fusion hybrid genotypes, viz., C31-17-5, C31-17-24, and C31-17-51, were used successfully as female parents in crosses with four tetraploid potato clones; cv Katahdin, cv Frieslander, 6704-1 and AM 66.42 (chapter 2). From these crosses, several BC 1 progenies were obtained. Among these, six BC 1 plants, viz., 6739, 2001, 2002, 2003, 2004 and 2005 were analysed through RFLP and GISH analyses. Due to preferential alien tomato chromosome elimination, a variable number of tomato chromosomes (6-11) was present in these genotypes. An important result was that all the 12 different tomato chromosomes were present in these six BC 1 progeny plants, albeit in different genotypes.By backcrossing three of these BC 1 plants to tetraploid potato, a total of 97 BC 2 plants were obtained and analysed through RFLP and GISH (chapter 3). The number of alien tomato chromosomes in these BC 2 plants varied from 0-6. The average rate of tomato chromosome transmission among different populations ranged from 1.7 to 3.4. Generally, a much higher rate of transmission was observed when the alien tomato chromosome in the BC 1 plant was in a disomic condition. A statistical analysis of the rate of female transmission could not prove an equal probability of chromosome transmission among the individual tomato chromosomes. A notable result of this investigation on the BC 2 plants was that among the 12 possible types of monosomic additions seven, viz., chromosome 1, 2, 4, 6, 8, 10 and 12, were identified. This clearly indicated that, despite the problem of selective elimination of the alien tomato chromosomes, it is possible to select genotypes that retain the alien chromosomes in a stable condition and transmit them through the gametes to the progeny.Besides monosomic additions, an occasional disomic was also detected through RFLP and GISH analyses (chapter 4). Remarkably, one of the chromosomes of this pair in the BC 2 plant 2101-1, was much smaller that the other. A detailed analysis of microsporogenesis indicated that at pachytene stage the large and small tomato chromosomes paired as homologues, and morphologically it was identified as chromosome 10 of tomato, which was also concurrent with RFLP analysis using chromosome specific probes. Morphologically, a deletion for the heterochromatic part of the long arm of the chromosome 10 of tomato had given rise to the smaller of this homologous pair. A detailed analysis of microsporogenesis indicated that a precocious disjunction and division of the alien pair of chromosomes was the cause of the origin of disomic additions in this material. It was evident from this study that the pachytene chromosomes of tomato can be clearly identified after GISH procedures even in a polyploid genotype and the deletion could be clearly characterised. In addition, a BC 3 population consisting of 96 plants was created and characterised through RFLP and GISH analyses after backcrossing the disomic addition to a tetraploid potato pollen parent. Therefore, the female transmission of both the normal and the aberrant homologues could be determined.To gain insight into homoeologous pairing and crossing-over between the potato and tomato chromosomes, a Somatic Fusion hybrid, C31-17-51, with two translocated chromosomes, and two genotypes, 2103-1 and 2301-2, with monosomic alien tomato additions for chromosome 1 and 8 respectively, were investigated (chapter 5). There was clear evidence for the presence of a reciprocal translocation in the Somatic Fusion hybrid. As a consequence, the quadrivalent formation involving potato and tomato chromosomes was only due to the autosyndetic pairing. In the absence of any knowledge of this translocation, the high degree of pairing, 86.0 %, could have been mistaken for allosyndetic pairing. An analysis of chromosome pairing and chiasmata formation in the monosomic additions indicated a very low frequency of homoeologous pairing, 1.3 and 1.1 % for the chromosome additions 1 and 8 respectively. Chiasma formation between homoeologues chromosomes was estimated to occur in 0.8 % of the meiotic cells studied in the monosomic alien addition for chromosome 8.The investigations presented in this thesis demonstrated that, although the frequency of homoeologous pairing is low, there is a prospect for introgressing tomato chromosomal DNA into the potato genome.

  • Introgression of tomato chromosomes into the potato genome : an analysis through molecular marker and in situ hybridisation techniques = [Introgressie van tomatenchromosomen in het aardappelgenoom : een analyse met behulp van moleculaire merker en in
    Garriga Caldere, 1998
    Co-Authors: Garriga Calderé F.
    Abstract:

    <p>Transfer of alien chromosomes and genes across intergeneric boundaries can be useful not only for the introgression of desirable characters but also for fundamental genetic studies. The successful demonstration of hybridisation of potato ( <em>Solanum tuberosum</em> ) and tomato ( <em>Lycopersicon esculentum</em> ) through protoplast Fusion in 1978, created the potential for introgressing chromosomes and genes from one genus into the other. However, real prospects of adding tomato chromosomes into the potato genome arose only after a potato (+) tomato Fusion hybrid was successfully backcrossed to tetraploid potato in 1994.</p><p>Despite this achievement, the problem of selective alien tomato chromosome elimination from the Fusion hybrid and the backcross progenies, as well as the behaviour of the alien tomato chromosomes in the potato background, raised the following questions: (i) in view of the selective elimination of alien tomato chromosomes, is it possible to establish a complete series of alien tomato chromosome additions in a potato background? (ii) do the introgressed alien chromosomes retain their structural integrity? (iii) can all the alien tomato chromosomes be transmitted through the gametes to the sexual progeny and if so, in what frequency? (iv) if there are any structural changes in the introgressed alien chromosomes, can they be identified and characterised? (v) do the potato and tomato chromosomes pair homoeologously and cross-over?</p><p>An attempt was made in the present investigation to create the appropriate plant material to answer the five above mentioned questions. In this connection, the chromosome constitution of the Somatic Fusion hybrids and their backcross derivatives, viz., BC <sub>1</sub> , BC <sub>2</sub> and BC <sub>3</sub> progenies were analysed through a combination of restriction fragment length polymorphism (RFLP) using tomato chromosome specific probes, genomic <em>in situ</em> hybridisation (GISH) and fluorescence <em>in situ</em> hybridisation (FISH) analyses.</p><p>In order to produce BC <sub>1</sub> progenies with the full potential for the creation of all the 12 possible monosomic tomato chromosome additions in a tetraploid potato background, three different hexaploid Somatic Fusion hybrid genotypes, viz., C31-17-5, C31-17-24, and C31-17-51, were used successfully as female parents in crosses with four tetraploid potato clones; cv Katahdin, cv Frieslander, 6704-1 and AM 66.42 (chapter 2). From these crosses, several BC <sub>1</sub> progenies were obtained. Among these, six BC <sub>1</sub> plants, viz., 6739, 2001, 2002, 2003, 2004 and 2005 were analysed through RFLP and GISH analyses. Due to preferential alien tomato chromosome elimination, a variable number of tomato chromosomes (6-11) was present in these genotypes. An important result was that all the 12 different tomato chromosomes were present in these six BC <sub>1</sub> progeny plants, albeit in different genotypes.</p><p>By backcrossing three of these BC <sub>1</sub> plants to tetraploid potato, a total of 97 BC <sub>2</sub> plants were obtained and analysed through RFLP and GISH (chapter 3). The number of alien tomato chromosomes in these BC <sub>2</sub> plants varied from 0-6. The average rate of tomato chromosome transmission among different populations ranged from 1.7 to 3.4. Generally, a much higher rate of transmission was observed when the alien tomato chromosome in the BC <sub>1</sub> plant was in a disomic condition. A statistical analysis of the rate of female transmission could not prove an equal probability of chromosome transmission among the individual tomato chromosomes. A notable result of this investigation on the BC <sub>2</sub> plants was that among the 12 possible types of monosomic additions seven, viz., chromosome 1, 2, 4, 6, 8, 10 and 12, were identified. This clearly indicated that, despite the problem of selective elimination of the alien tomato chromosomes, it is possible to select genotypes that retain the alien chromosomes in a stable condition and transmit them through the gametes to the progeny.</p><p>Besides monosomic additions, an occasional disomic was also detected through RFLP and GISH analyses (chapter 4). Remarkably, one of the chromosomes of this pair in the BC <sub>2</sub> plant 2101-1, was much smaller that the other. A detailed analysis of microsporogenesis indicated that at pachytene stage the large and small tomato chromosomes paired as homologues, and morphologically it was identified as chromosome 10 of tomato, which was also concurrent with RFLP analysis using chromosome specific probes. Morphologically, a deletion for the heterochromatic part of the long arm of the chromosome 10 of tomato had given rise to the smaller of this homologous pair. A detailed analysis of microsporogenesis indicated that a precocious disjunction and division of the alien pair of chromosomes was the cause of the origin of disomic additions in this material. It was evident from this study that the pachytene chromosomes of tomato can be clearly identified after GISH procedures even in a polyploid genotype and the deletion could be clearly characterised. In addition, a BC <sub>3</sub> population consisting of 96 plants was created and characterised through RFLP and GISH analyses after backcrossing the disomic addition to a tetraploid potato pollen parent. Therefore, the female transmission of both the normal and the aberrant homologues could be determined.</p><p>To gain insight into homoeologous pairing and crossing-over between the potato and tomato chromosomes, a Somatic Fusion hybrid, C31-17-51, with two translocated chromosomes, and two genotypes, 2103-1 and 2301-2, with monosomic alien tomato additions for chromosome 1 and 8 respectively, were investigated (chapter 5). There was clear evidence for the presence of a reciprocal translocation in the Somatic Fusion hybrid. As a consequence, the quadrivalent formation involving potato and tomato chromosomes was only due to the autosyndetic pairing. In the absence of any knowledge of this translocation, the high degree of pairing, 86.0 %, could have been mistaken for allosyndetic pairing. An analysis of chromosome pairing and chiasmata formation in the monosomic additions indicated a very low frequency of homoeologous pairing, 1.3 and 1.1 % for the chromosome additions 1 and 8 respectively. Chiasma formation between homoeologues chromosomes was estimated to occur in 0.8 % of the meiotic cells studied in the monosomic alien addition for chromosome 8.</p><p>The investigations presented in this thesis demonstrated that, although the frequency of homoeologous pairing is low, there is a prospect for introgressing tomato chromosomal DNA into the potato genome.</p>

M. Omura - One of the best experts on this subject based on the ideXlab platform.

  • analysis of mitochondrial genomes among citrus plants produced by the interspecific Somatic Fusion of seminole tangelo with rough lemon
    Plant Cell Reports, 1997
    Co-Authors: T. Moriguchi, Toshiaki Motomura, T. Hidaka, Tomoya Akihama, M. Omura
    Abstract:

    Interspecific Somatic Fusion was performed between ‘Seminole’ tangelo (Citrus reticulata Blanco xC. paradisi Macf.) protoplasts isolated from embryogenic callus and rough lemon (C. jambhiri Lush.) mesophyll protoplasts. Eight plants out of ten randomly selected regenerants had 18 chromosomes and the same nuclear rDNA fragment patterns as that of the mesophyll parent. The remaining two plants showed rDNA fragment patterns from both parents and had 36 chromosomes. For the analysis of mitochondrial DNA (mtDNA),rrn26 derived from pea was used to probeBamHI digests of the regenerants. All plants showed mtDNA band patterns identical to that of the callus parent, suggesting that eight plants were cybrids and the remaining two plants were Somatic hybrids. In addition to the callus parent band patterns, additional fragments from the mesophyll parent and/or a novel band fragment were revealed in some of the putative cybrids by peaatpA probe after digestion withDraI andPstI. These results suggest the occurrence of mtDNA recombination/rearrangement inCitrus cybrids produced by Somatic Fusion in this interspecific combination.

  • Analysis of mitochondrial genomes amongCitrus plants produced by the interspecific Somatic Fusion of ‘Seminole’ tangelo with rough lemon
    Plant Cell Reports, 1997
    Co-Authors: T. Moriguchi, Toshiaki Motomura, T. Hidaka, Tomoya Akihama, M. Omura
    Abstract:

    Interspecific Somatic Fusion was performed between ‘Seminole’ tangelo ( Citrus reticulata Blanco x C. paradisi Macf.) protoplasts isolated from embryogenic callus and rough lemon ( C. jambhiri Lush.) mesophyll protoplasts. Eight plants out of ten randomly selected regenerants had 18 chromosomes and the same nuclear rDNA fragment patterns as that of the mesophyll parent. The remaining two plants showed rDNA fragment patterns from both parents and had 36 chromosomes. For the analysis of mitochondrial DNA (mtDNA), rrn 26 derived from pea was used to probe Bam HI digests of the regenerants. All plants showed mtDNA band patterns identical to that of the callus parent, suggesting that eight plants were cybrids and the remaining two plants were Somatic hybrids. In addition to the callus parent band patterns, additional fragments from the mesophyll parent and/or a novel band fragment were revealed in some of the putative cybrids by pea atp A probe after digestion with Dra I and Pst I. These results suggest the occurrence of mtDNA recombination/rearrangement in Citrus cybrids produced by Somatic Fusion in this interspecific combination.

  • Analysis of mitochondrial genomes among Citrus plants produced by the interspecific Somatic Fusion of ‘Seminole’ tangelo with rough lemon
    Plant Cell Reports, 1997
    Co-Authors: T. Moriguchi, Toshiaki Motomura, T. Hidaka, Tomoya Akihama, M. Omura
    Abstract:

    Interspecific Somatic Fusion was performed between ‘Seminole’ tangelo (Citrus reticulata Blanco xC. paradisi Macf.) protoplasts isolated from embryogenic callus and rough lemon (C. jambhiri Lush.) mesophyll protoplasts. Eight plants out of ten randomly selected regenerants had 18 chromosomes and the same nuclear rDNA fragment patterns as that of the mesophyll parent. The remaining two plants showed rDNA fragment patterns from both parents and had 36 chromosomes. For the analysis of mitochondrial DNA (mtDNA),rrn26 derived from pea was used to probeBamHI digests of the regenerants. All plants showed mtDNA band patterns identical to that of the callus parent, suggesting that eight plants were cybrids and the remaining two plants were Somatic hybrids. In addition to the callus parent band patterns, additional fragments from the mesophyll parent and/or a novel band fragment were revealed in some of the putative cybrids by peaatpA probe after digestion withDraI andPstI. These results suggest the occurrence of mtDNA recombination/rearrangement inCitrus cybrids produced by Somatic Fusion in this interspecific combination.

  • Analysis of mitochondrial genomes amongCitrus plants produced by the interspecific Somatic Fusion of 'Seminole' tangelo with rough lemon.
    Plant cell reports, 1997
    Co-Authors: T. Moriguchi, Toshiaki Motomura, T. Hidaka, Tomoya Akihama, M. Omura
    Abstract:

    Interspecific Somatic Fusion was performed between 'Seminole' tangelo (Citrus reticulata Blanco xC. paradisi Macf.) protoplasts isolated from embryogenic callus and rough lemon (C. jambhiri Lush.) mesophyll protoplasts. Eight plants out of ten randomly selected regenerants had 18 chromosomes and the same nuclear rDNA fragment patterns as that of the mesophyll parent. The remaining two plants showed rDNA fragment patterns from both parents and had 36 chromosomes. For the analysis of mitochondrial DNA (mtDNA),rrn26 derived from pea was used to probeBamHI digests of the regenerants. All plants showed mtDNA band patterns identical to that of the callus parent, suggesting that eight plants were cybrids and the remaining two plants were Somatic hybrids. In addition to the callus parent band patterns, additional fragments from the mesophyll parent and/or a novel band fragment were revealed in some of the putative cybrids by peaatpA probe after digestion withDraI andPstI. These results suggest the occurrence of mtDNA recombination/rearrangement inCitrus cybrids produced by Somatic Fusion in this interspecific combination.

Adrian Leuchtmann - One of the best experts on this subject based on the ideXlab platform.

  • horizontal transmission persistence and competition capabilities of epichloe endophytes in hordelymus europaeus grass hosts using dual endophyte inocula
    Fungal Ecology, 2014
    Co-Authors: Martina Oberhofer, Adrian Leuchtmann
    Abstract:

    The Epichloae (Ascomycota, Clavicipitaceae) are endophytes of grasses consisting of haploid species, and heteroploid interspecific hybrids that are prevalent in many natural host grass populations. Hybrid formation may involve Somatic Fusion of different mycelia in planta, but this has never been tested. We used Hordelymus europaeus as an experimental system, which naturally hosts several hybrid and non-hybrid endophytes. Seedlings were inoculated with mixed inocula including a hybrid and a non-hybrid strain from H. europaeus, and two sexual Epichloe species from other hosts. Evaluation of endophyte infection 13 months after inoculation detected a high rate of multiple infections within tillers, but no novel hybrid endophyte. The hybrid strain was also found in plants that had not received such inoculum suggesting horizontal transmission. Epiphytic conidia detected on leaf tissues were responsible for contagious spread. This observation may offer an explanation why hybrid endophytes are prevalent in natural populations of H. europaeus.

  • horizontal transmission persistence and competition capabilities of epichloe endophytes in hordelymus europaeus grass hosts using dual endophyte inocula
    Fungal Ecology, 2014
    Co-Authors: Martina Oberhofer, Adrian Leuchtmann
    Abstract:

    The Epichloae (Ascomycota, Clavicipitaceae) are endophytes of grasses consisting of haploid species, and heteroploid interspecific hybrids that are prevalent in many natural host grass populations. Hybrid formation may involve Somatic Fusion of different mycelia in planta, but this has never been tested. We used Hordelymus europaeus as an experimental system, which naturally hosts several hybrid and non-hybrid endophytes. Seedlings were inoculated with mixed inocula including a hybrid and a non-hybrid strain from H. europaeus, and two sexual Epichloe species from other hosts. Evaluation of endophyte infection 13 months after inoculation detected a high rate of multiple infections within tillers, but no novel hybrid endophyte. The hybrid strain was also found in plants that had not received such inoculum suggesting horizontal transmission. Epiphytic conidia detected on leaf tissues were responsible for contagious spread. This observation may offer an explanation why hybrid endophytes are prevalent in natural populations of H. europaeus.

T. Moriguchi - One of the best experts on this subject based on the ideXlab platform.

  • analysis of mitochondrial genomes among citrus plants produced by the interspecific Somatic Fusion of seminole tangelo with rough lemon
    Plant Cell Reports, 1997
    Co-Authors: T. Moriguchi, Toshiaki Motomura, T. Hidaka, Tomoya Akihama, M. Omura
    Abstract:

    Interspecific Somatic Fusion was performed between ‘Seminole’ tangelo (Citrus reticulata Blanco xC. paradisi Macf.) protoplasts isolated from embryogenic callus and rough lemon (C. jambhiri Lush.) mesophyll protoplasts. Eight plants out of ten randomly selected regenerants had 18 chromosomes and the same nuclear rDNA fragment patterns as that of the mesophyll parent. The remaining two plants showed rDNA fragment patterns from both parents and had 36 chromosomes. For the analysis of mitochondrial DNA (mtDNA),rrn26 derived from pea was used to probeBamHI digests of the regenerants. All plants showed mtDNA band patterns identical to that of the callus parent, suggesting that eight plants were cybrids and the remaining two plants were Somatic hybrids. In addition to the callus parent band patterns, additional fragments from the mesophyll parent and/or a novel band fragment were revealed in some of the putative cybrids by peaatpA probe after digestion withDraI andPstI. These results suggest the occurrence of mtDNA recombination/rearrangement inCitrus cybrids produced by Somatic Fusion in this interspecific combination.

  • Analysis of mitochondrial genomes amongCitrus plants produced by the interspecific Somatic Fusion of ‘Seminole’ tangelo with rough lemon
    Plant Cell Reports, 1997
    Co-Authors: T. Moriguchi, Toshiaki Motomura, T. Hidaka, Tomoya Akihama, M. Omura
    Abstract:

    Interspecific Somatic Fusion was performed between ‘Seminole’ tangelo ( Citrus reticulata Blanco x C. paradisi Macf.) protoplasts isolated from embryogenic callus and rough lemon ( C. jambhiri Lush.) mesophyll protoplasts. Eight plants out of ten randomly selected regenerants had 18 chromosomes and the same nuclear rDNA fragment patterns as that of the mesophyll parent. The remaining two plants showed rDNA fragment patterns from both parents and had 36 chromosomes. For the analysis of mitochondrial DNA (mtDNA), rrn 26 derived from pea was used to probe Bam HI digests of the regenerants. All plants showed mtDNA band patterns identical to that of the callus parent, suggesting that eight plants were cybrids and the remaining two plants were Somatic hybrids. In addition to the callus parent band patterns, additional fragments from the mesophyll parent and/or a novel band fragment were revealed in some of the putative cybrids by pea atp A probe after digestion with Dra I and Pst I. These results suggest the occurrence of mtDNA recombination/rearrangement in Citrus cybrids produced by Somatic Fusion in this interspecific combination.

  • Analysis of mitochondrial genomes among Citrus plants produced by the interspecific Somatic Fusion of ‘Seminole’ tangelo with rough lemon
    Plant Cell Reports, 1997
    Co-Authors: T. Moriguchi, Toshiaki Motomura, T. Hidaka, Tomoya Akihama, M. Omura
    Abstract:

    Interspecific Somatic Fusion was performed between ‘Seminole’ tangelo (Citrus reticulata Blanco xC. paradisi Macf.) protoplasts isolated from embryogenic callus and rough lemon (C. jambhiri Lush.) mesophyll protoplasts. Eight plants out of ten randomly selected regenerants had 18 chromosomes and the same nuclear rDNA fragment patterns as that of the mesophyll parent. The remaining two plants showed rDNA fragment patterns from both parents and had 36 chromosomes. For the analysis of mitochondrial DNA (mtDNA),rrn26 derived from pea was used to probeBamHI digests of the regenerants. All plants showed mtDNA band patterns identical to that of the callus parent, suggesting that eight plants were cybrids and the remaining two plants were Somatic hybrids. In addition to the callus parent band patterns, additional fragments from the mesophyll parent and/or a novel band fragment were revealed in some of the putative cybrids by peaatpA probe after digestion withDraI andPstI. These results suggest the occurrence of mtDNA recombination/rearrangement inCitrus cybrids produced by Somatic Fusion in this interspecific combination.

  • Analysis of mitochondrial genomes amongCitrus plants produced by the interspecific Somatic Fusion of 'Seminole' tangelo with rough lemon.
    Plant cell reports, 1997
    Co-Authors: T. Moriguchi, Toshiaki Motomura, T. Hidaka, Tomoya Akihama, M. Omura
    Abstract:

    Interspecific Somatic Fusion was performed between 'Seminole' tangelo (Citrus reticulata Blanco xC. paradisi Macf.) protoplasts isolated from embryogenic callus and rough lemon (C. jambhiri Lush.) mesophyll protoplasts. Eight plants out of ten randomly selected regenerants had 18 chromosomes and the same nuclear rDNA fragment patterns as that of the mesophyll parent. The remaining two plants showed rDNA fragment patterns from both parents and had 36 chromosomes. For the analysis of mitochondrial DNA (mtDNA),rrn26 derived from pea was used to probeBamHI digests of the regenerants. All plants showed mtDNA band patterns identical to that of the callus parent, suggesting that eight plants were cybrids and the remaining two plants were Somatic hybrids. In addition to the callus parent band patterns, additional fragments from the mesophyll parent and/or a novel band fragment were revealed in some of the putative cybrids by peaatpA probe after digestion withDraI andPstI. These results suggest the occurrence of mtDNA recombination/rearrangement inCitrus cybrids produced by Somatic Fusion in this interspecific combination.