The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform
Ji-xin Cheng - One of the best experts on this subject based on the ideXlab platform.
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Microsecond scale vibrational Spectroscopic Imaging by multiplex stimulated Raman scattering microscopy
Light: Science & Applications, 2015Co-Authors: Chien-sheng Liao, Mikhail N Slipchenko, Ping Wang, Junjie Li, Robert Aaron Oglesbee, Ji-xin ChengAbstract:Vibrational Spectroscopic Imaging on a microsecond time scale has been realized using multiplex Raman microscopy. Real-time vibrational Spectroscopic Imaging holds out the promise of enabling cellular processes to be monitored without labeling, but this has been difficult to achieve because of the long acquisition times needed. Now, researchers at Purdue University in the USA have realized vibrational Spectroscopic Imaging by parallel detection of a spectrally dispersed stimulated Raman signal. Using a homemade system, they acquired Raman spectra in 32 microseconds with a detection sensitivity that is nearly shot-noise limited. The scientists illustrate the potential of the technique by using it to obtain compositional maps of lipid droplets in living single cells, observe intracellular retinoid metabolism, and discriminate between fat droplets and protein-rich organelles in a nematode. Real-time vibrational Spectroscopic Imaging is desired for monitoring cellular states and cellular processes in a label-free manner. Raman Spectroscopic Imaging of highly dynamic systems is inhibited by relatively slow spectral acquisition on millisecond to second scale. Here, we report microsecond scale vibrational Spectroscopic Imaging by lock-in free parallel detection of spectrally dispersed stimulated Raman scattering signal. Using a homebuilt tuned amplifier array, our method enables Raman spectral acquisition, within the window defined by the broadband pulse, at the speed of 32 µs and with close to shot-noise limited detection sensitivity. Incorporated with multivariate curve resolution analysis, our platform allows compositional mapping of lipid droplets in single live cells, observation of intracellular retinoid metabolism, discrimination of fat droplets from protein-rich organelles in Caenorhabditis elegans , spectral detection of fast flowing tumor cells and monitoring drug diffusion through skin tissue in vivo . The reported technique opens new opportunities for compositional analysis of cellular compartment in a microscope setting and high-throughput spectral profiling of single cells in a flow cytometer setting.
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microsecond scale vibrational Spectroscopic Imaging by multiplex stimulated raman scattering microscopy
Light-Science & Applications, 2015Co-Authors: Chien-sheng Liao, Mikhail N Slipchenko, Ping Wang, Junjie Li, Robert Aaron Oglesbee, Ji-xin ChengAbstract:Vibrational Spectroscopic Imaging on a microsecond time scale has been realized using multiplex Raman microscopy. Real-time vibrational Spectroscopic Imaging holds out the promise of enabling cellular processes to be monitored without labeling, but this has been difficult to achieve because of the long acquisition times needed. Now, researchers at Purdue University in the USA have realized vibrational Spectroscopic Imaging by parallel detection of a spectrally dispersed stimulated Raman signal. Using a homemade system, they acquired Raman spectra in 32 microseconds with a detection sensitivity that is nearly shot-noise limited. The scientists illustrate the potential of the technique by using it to obtain compositional maps of lipid droplets in living single cells, observe intracellular retinoid metabolism, and discriminate between fat droplets and protein-rich organelles in a nematode.
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Vibrational Spectroscopic Imaging of living systems: An emerging platform for biology and medicine
Science, 2015Co-Authors: Ji-xin Cheng, X. Sunney XieAbstract:Vibrational spectroscopy has been extensively applied to the study of molecules in gas phase, in condensed phase, and at interfaces. The transition from spectroscopy to Spectroscopic Imaging of living systems, which allows the spectrum of biomolecules to act as natural contrast, is opening new opportunities to reveal cellular machinery and to enable molecule-based diagnosis. Such a transition, however, involves more than a simple combination of spectrometry and microscopy. We review recent efforts that have pushed the boundary of the vibrational Spectroscopic Imaging field in terms of spectral acquisition speed, detection sensitivity, spatial resolution, and Imaging depth. We further highlight recent applications in functional analysis of single cells and in label-free detection of diseases.
Chien-sheng Liao - One of the best experts on this subject based on the ideXlab platform.
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microsecond scale vibrational Spectroscopic Imaging by multiplex stimulated raman scattering microscopy
Light-Science & Applications, 2015Co-Authors: Chien-sheng Liao, Mikhail N Slipchenko, Ping Wang, Junjie Li, Robert Aaron Oglesbee, Ji-xin ChengAbstract:Vibrational Spectroscopic Imaging on a microsecond time scale has been realized using multiplex Raman microscopy. Real-time vibrational Spectroscopic Imaging holds out the promise of enabling cellular processes to be monitored without labeling, but this has been difficult to achieve because of the long acquisition times needed. Now, researchers at Purdue University in the USA have realized vibrational Spectroscopic Imaging by parallel detection of a spectrally dispersed stimulated Raman signal. Using a homemade system, they acquired Raman spectra in 32 microseconds with a detection sensitivity that is nearly shot-noise limited. The scientists illustrate the potential of the technique by using it to obtain compositional maps of lipid droplets in living single cells, observe intracellular retinoid metabolism, and discriminate between fat droplets and protein-rich organelles in a nematode.
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Microsecond scale vibrational Spectroscopic Imaging by multiplex stimulated Raman scattering microscopy
Light: Science & Applications, 2015Co-Authors: Chien-sheng Liao, Mikhail N Slipchenko, Ping Wang, Junjie Li, Robert Aaron Oglesbee, Ji-xin ChengAbstract:Vibrational Spectroscopic Imaging on a microsecond time scale has been realized using multiplex Raman microscopy. Real-time vibrational Spectroscopic Imaging holds out the promise of enabling cellular processes to be monitored without labeling, but this has been difficult to achieve because of the long acquisition times needed. Now, researchers at Purdue University in the USA have realized vibrational Spectroscopic Imaging by parallel detection of a spectrally dispersed stimulated Raman signal. Using a homemade system, they acquired Raman spectra in 32 microseconds with a detection sensitivity that is nearly shot-noise limited. The scientists illustrate the potential of the technique by using it to obtain compositional maps of lipid droplets in living single cells, observe intracellular retinoid metabolism, and discriminate between fat droplets and protein-rich organelles in a nematode. Real-time vibrational Spectroscopic Imaging is desired for monitoring cellular states and cellular processes in a label-free manner. Raman Spectroscopic Imaging of highly dynamic systems is inhibited by relatively slow spectral acquisition on millisecond to second scale. Here, we report microsecond scale vibrational Spectroscopic Imaging by lock-in free parallel detection of spectrally dispersed stimulated Raman scattering signal. Using a homebuilt tuned amplifier array, our method enables Raman spectral acquisition, within the window defined by the broadband pulse, at the speed of 32 µs and with close to shot-noise limited detection sensitivity. Incorporated with multivariate curve resolution analysis, our platform allows compositional mapping of lipid droplets in single live cells, observation of intracellular retinoid metabolism, discrimination of fat droplets from protein-rich organelles in Caenorhabditis elegans , spectral detection of fast flowing tumor cells and monitoring drug diffusion through skin tissue in vivo . The reported technique opens new opportunities for compositional analysis of cellular compartment in a microscope setting and high-throughput spectral profiling of single cells in a flow cytometer setting.
Sergei G Kazarian - One of the best experts on this subject based on the ideXlab platform.
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Perspectives on infrared Spectroscopic Imaging from cancer diagnostics to process analysis
Spectrochimica acta. Part A Molecular and biomolecular spectroscopy, 2021Co-Authors: Sergei G KazarianAbstract:Abstract This perspective paper discusses the recent and potential developments in the application of infrared Spectroscopic Imaging, with a focus on Fourier transform infrared (FTIR) Spectroscopic Imaging. The current state-of-the-art has been briefly reported, that includes recent trends and advances in applications of FTIR Spectroscopic Imaging to biomedical systems. Here, some new opportunities for research in the biomedical field, particularly for cancer diagnostics, and also in the engineering field of process analysis; as well as challenges in FTIR Spectroscopic Imaging are discussed. Current and future prospects that will bring Spectroscopic Imaging technologies to the frontier of advanced medical diagnostics and to process analytics in engineering applications will be outlined in this opinion paper.
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Recent advances in the applications of vibrational Spectroscopic Imaging and mapping to pharmaceutical formulations
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 2018Co-Authors: Andrew V. Ewing, Sergei G KazarianAbstract:Vibrational Spectroscopic Imaging and mapping approaches have continued in their development and applications for the analysis of pharmaceutical formulations. Obtaining spatially resolved chemical information about the distribution of different components within pharmaceutical formulations is integral for improving the understanding and quality of final drug products. This review aims to summarise some key advances of these technologies over recent years, primarily since 2010. An overview of FTIR, NIR, terahertz Spectroscopic Imaging and Raman mapping will be presented to give a perspective of the current state-of-the-art of these techniques for studying pharmaceutical samples. This will include their application to reveal spatial information of components that reveals molecular insight of polymorphic or structural changes, behaviour of formulations during dissolution experiments, uniformity of materials and detection of counterfeit products. Furthermore, new advancements will be presented that demonstrate the continuing novel applications of Spectroscopic Imaging and mapping, namely in FTIR spectroscopy, for studies of microfluidic devices. Whilst much of the recently developed work has been reported by academic groups, examples of the potential impacts of utilising these Imaging and mapping technologies to support industrial applications have also been reviewed.
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infrared spectroscopy and Spectroscopic Imaging in forensic science
Analyst, 2017Co-Authors: Andrew V. Ewing, Sergei G KazarianAbstract:Infrared spectroscopy and Spectroscopic Imaging, are robust, label free and inherently non-destructive methods with a high chemical specificity and sensitivity that are frequently employed in forensic science research and practices. This review aims to discuss the applications and recent developments of these methodologies in this field. Furthermore, the use of recently emerged Fourier transform infrared (FT-IR) Spectroscopic Imaging in transmission, external reflection and Attenuated Total Reflection (ATR) modes are summarised with relevance and potential for forensic science applications. This Spectroscopic Imaging approach provides the opportunity to obtain the chemical composition of fingermarks and information about possible contaminants deposited at a crime scene. Research that demonstrates the great potential of these techniques for analysis of fingerprint residues, explosive materials and counterfeit drugs will be reviewed. The implications of this research for the examination of different materials are considered, along with an outlook of possible future research avenues for the application of vibrational Spectroscopic methods to the analysis of forensic samples.
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Enhancing forensic science with Spectroscopic Imaging
Optics and Photonics for Counterterrorism and Crime Fighting II, 2006Co-Authors: Camilla Ricci, Sergei G KazarianAbstract:This presentation outlines the research we are developing in the area of Fourier Transform Infrared (FTIR) Spectroscopic Imaging with the focus on materials of forensic interest. FTIR Spectroscopic Imaging has recently emerged as a powerful tool for characterisation of heterogeneous materials. FTIR Imaging relies on the ability of the military-developed infrared array detector to simultaneously measure spectra from thousands of different locations in a sample. Recently developed application of FTIR Imaging using an ATR (Attenuated Total Reflection) mode has demonstrated the ability of this method to achieve spatial resolution beyond the diffraction limit of infrared light in air. Chemical visualisation with enhanced spatial resolution in micro-ATR mode broadens the range of materials studied with FTIR Imaging with applications to pharmaceutical formulations or biological samples. Macro-ATR Imaging has also been developed for chemical Imaging analysis of large surface area samples and was applied to analyse the surface of human skin (e.g. finger), counterfeit tablets, textile materials (clothing), etc. This approach demonstrated the ability of this Imaging method to detect trace materials attached to the surface of the skin. This may also prove as a valuable tool in detection of traces of explosives left or trapped on the surfaces of different materials. This FTIR Imaging method is substantially superior to many of the other Imaging methods due to inherent chemical specificity of infrared spectroscopy and fast acquisition times of this technique. Our preliminary data demonstrated that this methodology will provide the means to non-destructive detection method that could relate evidence to its source. This will be important in a wider crime prevention programme. In summary, intrinsic chemical specificity and enhanced visualising capability of FTIR Spectroscopic Imaging open a window of opportunities for counter-terrorism and crime-fighting, with applications ranging from analysis of trace evidence (e.g. in soil), tablets, drugs, fibres, tape explosives, biological samples to detection of gunshot residues and Imaging of fingerprints.
Peter Boesiger - One of the best experts on this subject based on the ideXlab platform.
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Trading spectral separation at 3T for acquisition speed in multi spin-echo Spectroscopic Imaging.
AJNR. American journal of neuroradiology, 2006Co-Authors: Ulrike Dydak, Dieter Meier, Rolf Lamerichs, Peter BoesigerAbstract:Fast multiple spin-echo Spectroscopic Imaging, also called turbo Spectroscopic Imaging (TSI), may be enhanced in terms of acquisition speed by taking advantage of the higher spectral separation afforded at higher field strength and by further combining it with sensitivity encoding (SENSE). This article demonstrates the possibilities of this approach at 3T, resulting in scan-time reductions of up to a factor of 10. High-resolution, in vivo, single- and multiple-section Spectroscopic Imaging data are presented.
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Sensitivity-encoded Spectroscopic Imaging
Magnetic Resonance in Medicine, 2001Co-Authors: Ulrike Dydak, Markus Weiger, Dieter Meier, Klaas P. Pruessmann, Peter BoesigerAbstract:Sensitivity encoding (SENSE) offers a new, highly effective approach to reducing the acquisition time in Spectroscopic Imaging (SI). In contrast to conventional fast SI techniques, which accelerate k-space sampling, this method permits reducing the number of phase encoding steps in each phase encoding dimension of conventional SI. Using a coil array for data acquisition, the missing encoding information is recovered exploiting knowledge of the distinct spatial sensitivities of the individual coil elements. In this work, SENSE is applied to 2D Spectroscopic Imaging. Fourfold reduction of scan time is achieved at preserved spectral and spatial resolution, maintaining a reasonable SNR. The basic properties of the proposed method are demonstrated by phantom experiments. The in vivo feasibility of SENSE-SI is verified by metabolic Imaging of N-acetylaspartate, creatine, and choline in the human brain. These results are compared to conventional SI, with special attention to the spatial response and the SNR.
Junjie Li - One of the best experts on this subject based on the ideXlab platform.
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microsecond scale vibrational Spectroscopic Imaging by multiplex stimulated raman scattering microscopy
Light-Science & Applications, 2015Co-Authors: Chien-sheng Liao, Mikhail N Slipchenko, Ping Wang, Junjie Li, Robert Aaron Oglesbee, Ji-xin ChengAbstract:Vibrational Spectroscopic Imaging on a microsecond time scale has been realized using multiplex Raman microscopy. Real-time vibrational Spectroscopic Imaging holds out the promise of enabling cellular processes to be monitored without labeling, but this has been difficult to achieve because of the long acquisition times needed. Now, researchers at Purdue University in the USA have realized vibrational Spectroscopic Imaging by parallel detection of a spectrally dispersed stimulated Raman signal. Using a homemade system, they acquired Raman spectra in 32 microseconds with a detection sensitivity that is nearly shot-noise limited. The scientists illustrate the potential of the technique by using it to obtain compositional maps of lipid droplets in living single cells, observe intracellular retinoid metabolism, and discriminate between fat droplets and protein-rich organelles in a nematode.
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Microsecond scale vibrational Spectroscopic Imaging by multiplex stimulated Raman scattering microscopy
Light: Science & Applications, 2015Co-Authors: Chien-sheng Liao, Mikhail N Slipchenko, Ping Wang, Junjie Li, Robert Aaron Oglesbee, Ji-xin ChengAbstract:Vibrational Spectroscopic Imaging on a microsecond time scale has been realized using multiplex Raman microscopy. Real-time vibrational Spectroscopic Imaging holds out the promise of enabling cellular processes to be monitored without labeling, but this has been difficult to achieve because of the long acquisition times needed. Now, researchers at Purdue University in the USA have realized vibrational Spectroscopic Imaging by parallel detection of a spectrally dispersed stimulated Raman signal. Using a homemade system, they acquired Raman spectra in 32 microseconds with a detection sensitivity that is nearly shot-noise limited. The scientists illustrate the potential of the technique by using it to obtain compositional maps of lipid droplets in living single cells, observe intracellular retinoid metabolism, and discriminate between fat droplets and protein-rich organelles in a nematode. Real-time vibrational Spectroscopic Imaging is desired for monitoring cellular states and cellular processes in a label-free manner. Raman Spectroscopic Imaging of highly dynamic systems is inhibited by relatively slow spectral acquisition on millisecond to second scale. Here, we report microsecond scale vibrational Spectroscopic Imaging by lock-in free parallel detection of spectrally dispersed stimulated Raman scattering signal. Using a homebuilt tuned amplifier array, our method enables Raman spectral acquisition, within the window defined by the broadband pulse, at the speed of 32 µs and with close to shot-noise limited detection sensitivity. Incorporated with multivariate curve resolution analysis, our platform allows compositional mapping of lipid droplets in single live cells, observation of intracellular retinoid metabolism, discrimination of fat droplets from protein-rich organelles in Caenorhabditis elegans , spectral detection of fast flowing tumor cells and monitoring drug diffusion through skin tissue in vivo . The reported technique opens new opportunities for compositional analysis of cellular compartment in a microscope setting and high-throughput spectral profiling of single cells in a flow cytometer setting.
