Specular Microscopy

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Miguel J Maldonado - One of the best experts on this subject based on the ideXlab platform.

Tetsuro Oshika - One of the best experts on this subject based on the ideXlab platform.

  • central corneal thickness measurements using orbscan ii scanning slit topography noncontact Specular Microscopy and ultrasonic pachymetry in eyes with keratoconus
    Cornea, 2005
    Co-Authors: Keisuke Kawana, Kazunori Miyata, Tadatoshi Tokunaga, Takahiro Kiuchi, Takahiro Hiraoka, Tetsuro Oshika
    Abstract:

    PURPOSE: To compare corneal thickness measurements using Orbscan II scanning slit topography, Topcon SP-2000P noncontact Specular Microscopy, and ultrasonic pachymetry in eyes with keratoconus. METHODS: Central corneal thickness was measured in 22 eyes with keratoconus. Eyes with apparent corneal opacity were excluded. Scanning slit topography, noncontact Specular Microscopy, and ultrasonic pachymetry were used in this sequence. The acoustic equivalent correlation factor (0.92) was used for Orbscan readings. RESULTS: Three devices gave significantly different corneal thickness readings (P < 0.001, repeated-measure analysis of variance). Measurements with Orbscan scanning slit topography (449.5 +/- 43.2 [SD] mum) were significantly smaller than those of ultrasonic pachymetry (485.0 +/- 29.3 microm; P < 0.001, Tukey multiple comparison) and SP-2000P noncontact Specular Microscopy (476.7 +/- 28.3 microm; P = 0.002). There were significant linear correlations between ultrasonic pachymetry and scanning slit topography (Pearson correlation coefficient r = 0.741, P < 0.001), between scanning slit topography and noncontact Specular Microscopy (r = 0.880, P < 0.001), and between noncontact Specular Microscopy and ultrasonic pachymetry (r = 0.811, P < 0.001). CONCLUSION: In eyes with keratoconus, Orbscan II scanning slit topography system gave significantly smaller corneal thickness readings than the other 2 devices. Measurements taken by noncontact Specular Microscopy and ultrasonic pachymetry were comparable. Three devices showed significant linear correlations with one another.

  • comparison of corneal thickness measurements using orbscan ii non contact Specular Microscopy and ultrasonic pachymetry in eyes after laser in situ keratomileusis
    British Journal of Ophthalmology, 2004
    Co-Authors: Keisuke Kawana, Kazunori Miyata, Tadatoshi Tokunaga, Takahiro Kiuchi, Fumiki Okamoto, Tetsuro Oshika
    Abstract:

    Aims: To compare central corneal thickness measurements of three pachymetry devices in eyes after laser in situ keratomileusis (LASIK). Methods: Central corneal thickness was measured in 203 eyes after myopic LASIK. Orbscan II scanning slit topography (Bausch & Lomb), SP-2000P non-contact Specular Microscopy (Topcon), and ultrasonic pachymetry (Tomey) were used in this sequence. Results: Three devices gave significantly different corneal thickness readings (p r  = 0.912, p r  = 0.968, p r  = 0.933, p Conclusion: In post-LASIK eyes, Orbscan II scanning slit topography significantly underestimated corneal thickness. Non-contact Specular Microscopy gave smaller thickness readings than ultrasonic pachymetry, but these two units showed an excellent linear correlation.

  • corneal thickness measurements scanning slit corneal topography and noncontact Specular Microscopy versus ultrasonic pachymetry
    Journal of Cataract and Refractive Surgery, 2003
    Co-Authors: Shigenobu Suzuki, Tetsuro Oshika, Isao Sakabe, Aiko Iwase, Shiro Amano, Makoto Araie
    Abstract:

    Abstract Purpose To compare central corneal thickness measurements taken with 3 pachymetry systems: Orbscan scanning-slit corneal topography/pachymetry, Topcon SP2000P noncontact Specular Microscopy, and Tomey ultrasonic pachymetry. Setting Multicenter study, Tokyo, Japan. Methods In 216 healthy eyes of 114 subjects, scanning-slit topography, noncontact Specular Microscopy, and ultrasonic pachymetry were used in that sequence to record central corneal thickness. In another 20 healthy eyes of 13 subjects, 2 sets of measurements were repeated for each pachymetry to assess repeatability. Results The mean central corneal thickness was compatible between scanning-slit topography (546.9 μm ± 35.4 [SD] ) and ultrasonic pachymetry (548.1 ± 33.0 μm); however, noncontact Specular Microscopy gave a significantly smaller mean (525.3 ± 31.4 μm) than the other 2 tests (P Conclusions Corneal thickness readings were comparable between scanning-slit topography and pachymetry; noncontact Specular Microscopy gave significantly smaller values. The measurements of the 3 methods showed significant linear correlations with one another. All methods provided acceptable repeatability of measurements.

Alberto Lopezmiguel - One of the best experts on this subject based on the ideXlab platform.

Makoto Araie - One of the best experts on this subject based on the ideXlab platform.

  • corneal thickness measurements scanning slit corneal topography and noncontact Specular Microscopy versus ultrasonic pachymetry
    Journal of Cataract and Refractive Surgery, 2003
    Co-Authors: Shigenobu Suzuki, Tetsuro Oshika, Isao Sakabe, Aiko Iwase, Shiro Amano, Makoto Araie
    Abstract:

    Abstract Purpose To compare central corneal thickness measurements taken with 3 pachymetry systems: Orbscan scanning-slit corneal topography/pachymetry, Topcon SP2000P noncontact Specular Microscopy, and Tomey ultrasonic pachymetry. Setting Multicenter study, Tokyo, Japan. Methods In 216 healthy eyes of 114 subjects, scanning-slit topography, noncontact Specular Microscopy, and ultrasonic pachymetry were used in that sequence to record central corneal thickness. In another 20 healthy eyes of 13 subjects, 2 sets of measurements were repeated for each pachymetry to assess repeatability. Results The mean central corneal thickness was compatible between scanning-slit topography (546.9 μm ± 35.4 [SD] ) and ultrasonic pachymetry (548.1 ± 33.0 μm); however, noncontact Specular Microscopy gave a significantly smaller mean (525.3 ± 31.4 μm) than the other 2 tests (P Conclusions Corneal thickness readings were comparable between scanning-slit topography and pachymetry; noncontact Specular Microscopy gave significantly smaller values. The measurements of the 3 methods showed significant linear correlations with one another. All methods provided acceptable repeatability of measurements.

Roy S Chuck - One of the best experts on this subject based on the ideXlab platform.

  • technician consistency in Specular Microscopy measurements a real world retrospective analysis of a united states eye bank
    Cornea, 2017
    Co-Authors: Gabriel M Rand, Ji Won Kwon, Patrick Gore, Mitchell D Mccartney, Roy S Chuck
    Abstract:

    PURPOSE: To quantify consistency of endothelial cell density (ECD) measurements among technicians in a single US eye bank operating under typical operating conditions. METHODS: In this retrospective analysis of 51 Microscopy technicians using a semiautomated counting method on 35,067 eyes from July 2007 to May 2015, technician- and date-related marginal ECD effects were calculated using linear regression models. ECD variance was correlated with the number of Specular Microscopy technicians. RESULTS: Technician mean ECDs ranged from 2386 ± 431 to 3005 ± 560 cells/mm. Nine technicians had statistically and clinically significant marginal effects. Annual mean ECDs adjusted for changes in technicians ranged from 2422 ± 433 to 2644 ± 430 cells/mm. The period of 2007 to 2009 had statistically and clinically significant marginal effects. There was a nonstatistically significant association between the number of technicians and ECD standard deviation. CONCLUSIONS: There was significant ECD variability associated with Specular Microscopy technicians and with the date of measurement. We recommend that eye banks collect data related to laboratory factors that have been shown to influence ECD variability.

  • Technician Consistency in Specular Microscopy Measurements: A “Real-World” Retrospective Analysis of a United States Eye Bank
    Cornea, 2017
    Co-Authors: Gabriel M Rand, Ji Won Kwon, Patrick Gore, Mitchell D Mccartney, Roy S Chuck
    Abstract:

    PURPOSE: To quantify consistency of endothelial cell density (ECD) measurements among technicians in a single US eye bank operating under typical operating conditions. METHODS: In this retrospective analysis of 51 Microscopy technicians using a semiautomated counting method on 35,067 eyes from July 2007 to May 2015, technician- and date-related marginal ECD effects were calculated using linear regression models. ECD variance was correlated with the number of Specular Microscopy technicians. RESULTS: Technician mean ECDs ranged from 2386 ± 431 to 3005 ± 560 cells/mm. Nine technicians had statistically and clinically significant marginal effects. Annual mean ECDs adjusted for changes in technicians ranged from 2422 ± 433 to 2644 ± 430 cells/mm. The period of 2007 to 2009 had statistically and clinically significant marginal effects. There was a nonstatistically significant association between the number of technicians and ECD standard deviation. CONCLUSIONS: There was significant ECD variability associated with Specular Microscopy technicians and with the date of measurement. We recommend that eye banks collect data related to laboratory factors that have been shown to influence ECD variability.