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Domenico Otranto - One of the best experts on this subject based on the ideXlab platform.
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First report of Thelazia callipaeda in a free-ranging Iberian wolf (Canis lupus signatus) from Spain.
Parasitology research, 2020Co-Authors: Fernando Nájera, Domenico Otranto, Jesús Lucas-veguillas, Ángel Vela, Manuel López-fernández, Pedro Martínez-martínez, Manuel Mata-huete, Javier Cáceres-urones, Giada Annoscia, Rafael Calero-bernalAbstract:Thelazia callipaeda (Spirurida, Thelaziidae) is a vector-borne zoonotic eyeworm able to infect a broad spectrum of carnivores. Here, we describe the first case of bilateral infection by T. callipaeda in the eyes of an adult female Iberian wolf (Canis lupus signatus) in central Spain. Nematodes collected were morphologically identified (n = 42), and two specimens were molecularly characterized. At the sequence analysis of the partial mitochondrial cytochrome c oxidase subunit 1 gene, T. callipaeda haplotype 1 (the only haplotype circulating in Europe) was detected. The role of the Iberian wolf as a natural reservoir for T. callipaeda in the life cycle of this emerging zoonosis and the implications in conservation are discussed.
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First report of Thelazia callipaeda in a free-ranging Iberian wolf (Canis lupus signatus) from Spain
Parasitology Research, 2020Co-Authors: Fernando Nájera, Domenico Otranto, Jesús Lucas-veguillas, Ángel Vela, Manuel López-fernández, Pedro Martínez-martínez, Manuel Mata-huete, Javier Cáceres-urones, Giada Annoscia, Rafael Calero-bernalAbstract:Thelazia callipaeda (Spirurida, Thelaziidae) is a vector-borne zoonotic eyeworm able to infect a broad spectrum of carnivores. Here, we describe the first case of bilateral infection by T. callipaeda in the eyes of an adult female Iberian wolf ( Canis lupus signatus ) in central Spain. Nematodes collected were morphologically identified ( n = 42), and two specimens were molecularly characterized. At the sequence analysis of the partial mitochondrial cytochrome c oxidase subunit 1 gene, T. callipaeda haplotype 1 (the only haplotype circulating in Europe) was detected. The role of the Iberian wolf as a natural reservoir for T. callipaeda in the life cycle of this emerging zoonosis and the implications in conservation are discussed.
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spirocerca vulpis sp nov Spiruridae spirocercidae description of a new nematode species of the red fox vulpes vulpes carnivora canidae
Parasitology, 2018Co-Authors: Alicia Rojas, Domenico Otranto, Gloria Sanchismonsonis, Amer Alic, Adnan Hodžic, Daniel Yasurlandau, Carlos Martinezcarrasco, Gad BanethAbstract:Previous studies have reported nematodes of the Spirocercidae family in the stomach nodules of red foxes (Vulpes vulpes) described as Spirocerca sp. or Spirocerca lupi (Rudolphi, 1819). We characterized spirurid worms collected from red foxes and compared them to S. lupi from domestic dogs by morphometric and phylogenetic analyses. Nematodes from red foxes differed from S. lupi by the presence of six triangular teeth-like buccal capsule structures, which are absent in the latter. Additionally, in female worms from red foxes, the distance of the vulva opening to the anterior end and the ratio of the glandular-to-muscular oesophagus lengths were larger than those of S. lupi (P < 0.006). In males, the lengths of the whole oesophagus and glandular part, the ratio of the glandular-to-muscular oesophagus and the comparison of the oesophagus to the total body length were smaller in S. lupi (all P < 0.044). Phylogenetic analyses revealed that S. lupi and the red foxes spirurid represent monophyletic sister groups with pairwise nucleotide distances of 9.2 and 0.2% in the cytochrome oxidase 1 and 18S genes, respectively. Based on these comparisons, the nematodes from red foxes were considered to belong to a separate species, for which the name Spirocerca vulpis sp. nov. is proposed.
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the eyeworm thelazia callipaeda in portugal current status of infection in pets and wild mammals and case report in a beech marten martes foina
Veterinary Parasitology, 2018Co-Authors: Fernanda Seixas, Paulo Travassos, Teresa A Coutinho, Ana Patricia Lopes, Maria Stefania Latrofa, M A Pires, Luis Cardoso, Domenico OtrantoAbstract:Abstract Ocular thelaziosis is caused by nematodes of the genus Thelazia (Spirurida, Thelaziidae), which inhabit the surface of the eyes and associated tissues. Thelazia callipaeda affects a range of mammal species, including humans, and in the last two decades has been reported in multiple European countries, being classified as an emergent vector-borne pathogen. In Portugal T. callipaeda is endemic in north-eastern areas, where it has been reported in domestic dogs, cats, red foxes (Vulpes vulpes) and wild European rabbits (Oryctolagus cuniculus). We report, for the first time, T. callipaeda in a beech marten (Martes foina) from Portugal and highlight the presence of haplotype 1 as the only one found in Europe, irrespective of the host species and geographical area of provenience.
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Additional file 3: of Phylogenetic analysis of Spirocerca lupi and Spirocerca vulpis reveal high genetic diversity and intra-individual variation
2018Co-Authors: Alicia Rojas, Adrienn Markovics, Domenico Otranto, Eran Dvir, Rรณbert Farkas, Kalyan Sarma, Sonjoy Borthakur, Abdul Jabbar, Gad BanethAbstract:Table S1. Pairwise nucleotide distance of the 18S gene (1611 bp fragment) expressed as percentages between specimens of Spirocerca spp. and other nematodes of the order Spirurida. (DOCX 18 kb
Rafael Calero-bernal - One of the best experts on this subject based on the ideXlab platform.
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First report of Thelazia callipaeda in a free-ranging Iberian wolf (Canis lupus signatus) from Spain
Parasitology Research, 2020Co-Authors: Fernando Nájera, Domenico Otranto, Jesús Lucas-veguillas, Ángel Vela, Manuel López-fernández, Pedro Martínez-martínez, Manuel Mata-huete, Javier Cáceres-urones, Giada Annoscia, Rafael Calero-bernalAbstract:Thelazia callipaeda (Spirurida, Thelaziidae) is a vector-borne zoonotic eyeworm able to infect a broad spectrum of carnivores. Here, we describe the first case of bilateral infection by T. callipaeda in the eyes of an adult female Iberian wolf ( Canis lupus signatus ) in central Spain. Nematodes collected were morphologically identified ( n = 42), and two specimens were molecularly characterized. At the sequence analysis of the partial mitochondrial cytochrome c oxidase subunit 1 gene, T. callipaeda haplotype 1 (the only haplotype circulating in Europe) was detected. The role of the Iberian wolf as a natural reservoir for T. callipaeda in the life cycle of this emerging zoonosis and the implications in conservation are discussed.
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First report of Thelazia callipaeda in a free-ranging Iberian wolf (Canis lupus signatus) from Spain.
Parasitology research, 2020Co-Authors: Fernando Nájera, Domenico Otranto, Jesús Lucas-veguillas, Ángel Vela, Manuel López-fernández, Pedro Martínez-martínez, Manuel Mata-huete, Javier Cáceres-urones, Giada Annoscia, Rafael Calero-bernalAbstract:Thelazia callipaeda (Spirurida, Thelaziidae) is a vector-borne zoonotic eyeworm able to infect a broad spectrum of carnivores. Here, we describe the first case of bilateral infection by T. callipaeda in the eyes of an adult female Iberian wolf (Canis lupus signatus) in central Spain. Nematodes collected were morphologically identified (n = 42), and two specimens were molecularly characterized. At the sequence analysis of the partial mitochondrial cytochrome c oxidase subunit 1 gene, T. callipaeda haplotype 1 (the only haplotype circulating in Europe) was detected. The role of the Iberian wolf as a natural reservoir for T. callipaeda in the life cycle of this emerging zoonosis and the implications in conservation are discussed.
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First Report of Thelazia callipaeda in Wildlife from Spain
Journal of wildlife diseases, 2013Co-Authors: Rafael Calero-bernal, Domenico Otranto, Juan Enrique Pérez-martín, F. J. Serrano, D. ReinaAbstract:We describe the first cases of infection by the nematode, Thelazia callipaeda (Spirurida, Thelaziidae) haplotype 1 in two red foxes (Vulpes vulpes) in Spain and discuss the potential role of red foxes as a reservoir for T. callipaeda.
Giada Annoscia - One of the best experts on this subject based on the ideXlab platform.
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First report of Thelazia callipaeda in a free-ranging Iberian wolf (Canis lupus signatus) from Spain
Parasitology Research, 2020Co-Authors: Fernando Nájera, Domenico Otranto, Jesús Lucas-veguillas, Ángel Vela, Manuel López-fernández, Pedro Martínez-martínez, Manuel Mata-huete, Javier Cáceres-urones, Giada Annoscia, Rafael Calero-bernalAbstract:Thelazia callipaeda (Spirurida, Thelaziidae) is a vector-borne zoonotic eyeworm able to infect a broad spectrum of carnivores. Here, we describe the first case of bilateral infection by T. callipaeda in the eyes of an adult female Iberian wolf ( Canis lupus signatus ) in central Spain. Nematodes collected were morphologically identified ( n = 42), and two specimens were molecularly characterized. At the sequence analysis of the partial mitochondrial cytochrome c oxidase subunit 1 gene, T. callipaeda haplotype 1 (the only haplotype circulating in Europe) was detected. The role of the Iberian wolf as a natural reservoir for T. callipaeda in the life cycle of this emerging zoonosis and the implications in conservation are discussed.
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First report of Thelazia callipaeda in a free-ranging Iberian wolf (Canis lupus signatus) from Spain.
Parasitology research, 2020Co-Authors: Fernando Nájera, Domenico Otranto, Jesús Lucas-veguillas, Ángel Vela, Manuel López-fernández, Pedro Martínez-martínez, Manuel Mata-huete, Javier Cáceres-urones, Giada Annoscia, Rafael Calero-bernalAbstract:Thelazia callipaeda (Spirurida, Thelaziidae) is a vector-borne zoonotic eyeworm able to infect a broad spectrum of carnivores. Here, we describe the first case of bilateral infection by T. callipaeda in the eyes of an adult female Iberian wolf (Canis lupus signatus) in central Spain. Nematodes collected were morphologically identified (n = 42), and two specimens were molecularly characterized. At the sequence analysis of the partial mitochondrial cytochrome c oxidase subunit 1 gene, T. callipaeda haplotype 1 (the only haplotype circulating in Europe) was detected. The role of the Iberian wolf as a natural reservoir for T. callipaeda in the life cycle of this emerging zoonosis and the implications in conservation are discussed.
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Cercopithifilaria sp. II in Vulpes vulpes: new host affiliation for an enigmatic canine filarioid.
Parasitology research, 2016Co-Authors: Carla Maia, Giada Annoscia, Maria Stefania Latrofa, María Casero, Vito Colella, André Pereira, Fábia Azevedo, Domenico OtrantoAbstract:Cercopithifilaria bainae and Cercopithifilaria grassii (Spirurida, Onchocercidae) are filarioids inhabiting the skin of dogs worldwide. The microfilariae of a third species namely, Cercopithifilaria sp. II sensu Otranto et al. 2013, have been morphologically and molecularly characterized but scientific knowledge of this parasite is minimal. The first case of infection of a red fox (Vulpes vulpes) with the filarioid Cercopithifilaria sp. II is herein described in Castro Marim, Portugal. Microfilariae from skin sediment of the fox’s ear were morphological characterized, and the identification was confirmed molecularly in samples from skin sediment, skin samples, and from Rhipicephalus sanguineus group ticks collected from the animal (99% homology with Cercopithifilaria sp. II). Studies should evaluate if red foxes might play a role in the maintenance and distribution of Cercopithifilaria sp. II infection in dog populations.
Donato Traversa - One of the best experts on this subject based on the ideXlab platform.
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Phylogenetic relationships of Habronema microstoma and Habronema muscae (Spirurida: Habronematidae) within the order Spirurida inferred using mitochondrial cytochrome c oxidase subunit 1 (cox1) gene analysis
Parasitology Research, 2008Co-Authors: Raffaella Iorio, Jan Šlapeta, Domenico Otranto, Barbara Paoletti, Annunziata Giangaspero, Donato TraversaAbstract:The present study investigated genetic variability within a population of Habronema microstoma and Habronema muscae (Spirurida: Habronematidae) affecting horses in an endemic area of central Italy using polymerase chain reaction (PCR)-coupled sequencing of the mitochondrial cytochrome c oxidase subunit 1 gene ( cox1 ). No different cox1 sequences were detected in any of the H. muscae individual, while two haplotypes representing H. microstoma individuals differed for one substitution. The pairwise distance between the H. muscae and H. microstoma was 11%, coding for five amino acid changes. The sequence of an informative region within the cox1 gene of H. microstoma and H. muscae was analyzed by Maximum Likelihood and Bayesian phylogenetic methods using available mitochondrial sequences spirurid taxa belonging to Filarioidea, Thelazioidea, and Habronematoidea. Phylogenetic analysis supported the split of the tree into two sister spirurid groups, Habronematoidea and Filarioidea + Thelazioidea. The phylogenetic and evolutionary implications of Habronema with Filaroidea and Thelazioidea are discussed.
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Molecular diagnosis of equid summer sores.
Veterinary parasitology, 2007Co-Authors: Donato Traversa, Raffaella Iorio, Annunziata Giangaspero, Lucio Petrizzi, Ippolito De Amicis, Sabine Brandt, Aranzazu Meana, Domenico OtrantoAbstract:Equine cutaneous habronemosis, also known as "summer sores", is a parasitic infection caused by larvae of Habronema microstoma and Habronema muscae (Nematoda, Spirurida) released by dung-inhabiting fly vectors on abraded skin, skin wounds or muco-cutaneous transition sites. Larvae induce a local inflammatory reaction characterised by itching, granulomatous, ulcerated and, often non-healing, lesions. The diagnosis of summer sores may be unreliable mainly because of the limits of clinical and microscopic examination. The applicability of a semi-nested PCR assay developed for the diagnosis of gastric habronemosis has been herein demonstrated for the detection of the cutaneous infection. The potential applicability of this diagnostic tool may have for clinical and epidemiological studies of cutaneous habronemosis in equids is discussed.
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mitochondrial cytochrome c oxidase subunit 1 cox1 gene sequence of spirocerca lupi nematoda Spirurida avenues for potential implications
Veterinary Parasitology, 2007Co-Authors: Donato Traversa, Francesca Costanzo, Raffaella Iorio, Itamar Aroch, Eran LavyAbstract:Canine spirocercosis is a life-threatening parasitosis caused by Spirocerca lupi (Nematoda, Spirurida) that is presently emerging in several countries. This study characterised an informative region within the mitochondrial (mtDNA) gene encoding for the cytochrome c oxidase subunit 1 (cox1) of S. lupi by Polymerase Chain Reaction (PCR)-coupled sequencing. Specimens from five different countries in Europe, Asia and Africa were examined and two different sequence variants of cox1 (i.e. haplotypes) were determined, displaying nucleotidic variation at 6 of 689 positions. All of these positions were invariable among all the parasite individuals from Europe (haplotype 1) and among the African and Asian individuals (haplotype 2), but differed between Europe and Asia/Africa. The S. lupi cox1 sequences were consistent with those of other common Spirurida previously reported at both nucleotidic and phylogenetic levels. This study provides molecular information essential for identification of the nematode, irrespective of its life cycle stage. Crucial implications for the specific molecular diagnosis of clinical spirocercosis and investigation of the evolution, population genetics, ecology and epidemiology of S. lupi are discussed.
Fernando Nájera - One of the best experts on this subject based on the ideXlab platform.
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First report of Thelazia callipaeda in a free-ranging Iberian wolf (Canis lupus signatus) from Spain
Parasitology Research, 2020Co-Authors: Fernando Nájera, Domenico Otranto, Jesús Lucas-veguillas, Ángel Vela, Manuel López-fernández, Pedro Martínez-martínez, Manuel Mata-huete, Javier Cáceres-urones, Giada Annoscia, Rafael Calero-bernalAbstract:Thelazia callipaeda (Spirurida, Thelaziidae) is a vector-borne zoonotic eyeworm able to infect a broad spectrum of carnivores. Here, we describe the first case of bilateral infection by T. callipaeda in the eyes of an adult female Iberian wolf ( Canis lupus signatus ) in central Spain. Nematodes collected were morphologically identified ( n = 42), and two specimens were molecularly characterized. At the sequence analysis of the partial mitochondrial cytochrome c oxidase subunit 1 gene, T. callipaeda haplotype 1 (the only haplotype circulating in Europe) was detected. The role of the Iberian wolf as a natural reservoir for T. callipaeda in the life cycle of this emerging zoonosis and the implications in conservation are discussed.
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First report of Thelazia callipaeda in a free-ranging Iberian wolf (Canis lupus signatus) from Spain.
Parasitology research, 2020Co-Authors: Fernando Nájera, Domenico Otranto, Jesús Lucas-veguillas, Ángel Vela, Manuel López-fernández, Pedro Martínez-martínez, Manuel Mata-huete, Javier Cáceres-urones, Giada Annoscia, Rafael Calero-bernalAbstract:Thelazia callipaeda (Spirurida, Thelaziidae) is a vector-borne zoonotic eyeworm able to infect a broad spectrum of carnivores. Here, we describe the first case of bilateral infection by T. callipaeda in the eyes of an adult female Iberian wolf (Canis lupus signatus) in central Spain. Nematodes collected were morphologically identified (n = 42), and two specimens were molecularly characterized. At the sequence analysis of the partial mitochondrial cytochrome c oxidase subunit 1 gene, T. callipaeda haplotype 1 (the only haplotype circulating in Europe) was detected. The role of the Iberian wolf as a natural reservoir for T. callipaeda in the life cycle of this emerging zoonosis and the implications in conservation are discussed.