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Mohamed A. Dkhil - One of the best experts on this subject based on the ideXlab platform.
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indigofera oblongifolia protects against trypanosomiasis induced Spleen Injury
2019Co-Authors: Mohamed A. Dkhil, Taghreed A Hafiz, Felwa A Thagfan, Esam M Alshaebi, Murad A Mubaraki, Mona F Khalil, Rewaida Abdelgaber, Saleh AlquraishyAbstract:Abstract Background Trypanosomiasis is a neglected tropical disease, transmitted by blood-sucking insects and can affect humans and animals, depending on the species of Trypanosoma parasite. Trypanosoma has acquired resistance to the majority of drugs used; hence, alternative medicines are required. Indigofera oblongifolia leaf extract (IOE) has been shown to treat blood stage malaria. Here, IOE was used to demonstrate its effect on Trypanosoma evansi-infected mice. Methods Analysis of IOE by gas chromatography–mass spectrometry showed the presence of many active components like flavonoids and phenolics. The mice were divided into three groups as follows: vehicle control, T. evansi-infected mice and T. evansi-infected-treated mice. Results The findings demonstrate a significant effect of IOE treatment on T. evansi-infected mice. Parasitemia was decreased by 70%, weight loss was reduced, and splenomegaly was significantly decreased. Additionally, IOE improved the histological architecture of the Spleen, as shown by the improved histological Injury score post-treatment. Anemia was apparent during the course of infection in T. evansi-infected mice; this was reversed upon treatment with IOE to almost the normal level of hemoglobin and erythrocytes. Reduced glutathione and catalase were also ameliorated upon IOE treatment compared to T. evansi-infected mice. Conclusion Overall, this study shows the ameliorative role of IOE against T. evansi-induced Spleen Injury in mice.
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ziziphus spina christi leaf extract pretreatment inhibits liver and Spleen Injury in a mouse model of sepsis via anti oxidant and anti inflammatory effects
2018Co-Authors: Mohamed A. Dkhil, Saleh Alquraishy, Ahmed Abdel E MoneimAbstract:: Sepsis is a systemic response to infection that can result in acute hepatic and splenic damage. Ziziphus spina-christi (L.) is a wild tree used as a medicinal plant by ancient Egyptians. However, little is known about the mechanism underlying its effects on sepsis. The current study investigated the protective effects of a Z. spina-christi leaf extract (ZSCLE) on liver and Spleen damage in a male C57BL/6 mouse model of sepsis, induced by cecal ligation and puncture (CLP). Prior to CLP, ZSCLE was administered daily for five consecutive days via oral gavage at doses of 100, 200, or 300 mg/kg. The mice were euthanized 9 h after CLP, and oxidative stress markers were measured (myeloperoxidase, lipid peroxidation, nitric oxide, and reduced glutathione). In addition, we investigated histological changes, anti-oxidant enzyme activities (superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase), cytokine levels, protein expression of nuclear factor-κB and inducible nitric oxide synthase (iNOS), and mRNA levels of mitogen-activated protein kinase (8, 9, and 14), iNOS, tumor necrosis factor-α, and interleukin-1β. Our results indicated that ZSCLE significantly and dose-dependently inhibited sepsis-induced liver and Spleen Injury. These results suggest that ZSCLE could provide a therapeutic agent for sepsis by inducing anti-inflammatory and anti-oxidant effects.
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beneficial effect of punica granatum peel extract on murine malaria induced Spleen Injury
2016Co-Authors: Murad A Mubaraki, Mohamed A. Dkhil, Taghreed A Hafiz, Saleh AlquraishyAbstract:Multiple drug-resistant malaria parasites have been widely detected, which has encouraged research studies focused on discovering alternative therapies. Medicinal plants such as pomegranate, Punica granatum, have been proven to exhibit antiprotozoal effects and therefore, we examined its effects on murine malaria-induced splenic Injury and oxidative stress in this study. Mice were divided into three groups, a vehicle control and two groups that were infected with 106 Plasmodium chabaudi-parasitized red blood cells (RBCs). The third group was gavaged with 100 μL of 300 mg/kg pomegranate peel extract for 6 days. All mice were euthanized on day 6 post-infection. The results revealed the potential antimalarial, antioxidant, and anti-inflammatory effects of pomegranate. Furthermore, pomegranate peel extracts significantly reduced parasitemia and Spleen index of the treated mice compared to the untreated group. Additionally, the Spleen histology score supported the findings by showing better amelioration in the pomegranate-treated mice than in the untreated mice. Concomitantly, the Spleen capsule thickness showed clear evidence of splenomegaly in the untreated mice, as evidenced by the reduced Spleen capsule. However, pomegranate peel extract exhibited a remarkable restorative effect on the Spleen capsules of the treated mice. Moreover, the extract significantly reduced the expression levels of the proinflammatory cytokines interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ as well as inducible nitric oxide synthase (iNOS). Moreover, our study showed that pomegranate extract profoundly affected oxidative stress levels by reducing the oxidant molecules, nitric oxide (NO) and malondialdehyde (MDA). This study showed that pomegranate clearly induced antimalarial activity in the host by attenuating inflammatory and oxidative stress responses. Furthermore, pomegranate enhanced the innate immune responses and, therefore, could serve an alternative therapy to control clinical malaria episodes and may protect against malaria infection.
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antimalarial and antioxidant activities of indigofera oblongifolia on plasmodium chabaudi induced Spleen tissue Injury in mice
2015Co-Authors: Mahmoud Y. Lubbad, Mohamed A. Dkhil, Saleh AlquraishyAbstract:Malaria is still one of the most common infectious diseases and leads to various public health problems worldwide. Medicinal plants are promising sources for identifying novel agents with potential antimalarial activity. This study aimed to investigate the antimalarial and the antioxidant activities of Indigofera oblongifolia on Plasmodium chabaudi-induced Spleen tissue Injury in mice. Mice were divided into five groups. The first group served as a vehicle control; the second, third, fourth, and fifth groups were infected with 1 × 106 P. chabaudi-parasitized erythrocytes. Mice of the last three groups were gavaged with 100 μl of I. oblongifolia leave extract (IOLE) at a dose of 100, 200, and 300 mg IOLE/kg, respectively, once daily for 7 days. IOLE was significantly able to lower the percentage of parasitemia. The most effective dose was the 100 mg IOLE/kg, which could reduce the parasitemia from about 38 to 12 %. The infection induced Spleen Injury. This was evidenced by disorganization of Spleen white and red pulps, appearance of hemozoin granules and parasitized erythrocytes. These changes in Spleen led to the increased histological score. Also, the infection increased the Spleen oxidative damage where the levels of nitrite/nitrate, malondialdehyde, and catalase were significantly altered. All these infection-induced parameters were significantly improved during IOLE treatment. In addition, the mRNA expression of inflammatory cytokines interleukin-1beta, interleukin-6, and tumor necrosis factor-alpha were upregulated after infection with P. chabaudi, whereas IOLE significantly reduced the expression of these genes. Our results indicate that I. oblongifolia leaves extract exhibits a significant antimalarial and antioxidant effects, and protects host Spleen tissue from injuries induced by P. chabaudi.
Saleh Alquraishy - One of the best experts on this subject based on the ideXlab platform.
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indigofera oblongifolia protects against trypanosomiasis induced Spleen Injury
2019Co-Authors: Mohamed A. Dkhil, Taghreed A Hafiz, Felwa A Thagfan, Esam M Alshaebi, Murad A Mubaraki, Mona F Khalil, Rewaida Abdelgaber, Saleh AlquraishyAbstract:Abstract Background Trypanosomiasis is a neglected tropical disease, transmitted by blood-sucking insects and can affect humans and animals, depending on the species of Trypanosoma parasite. Trypanosoma has acquired resistance to the majority of drugs used; hence, alternative medicines are required. Indigofera oblongifolia leaf extract (IOE) has been shown to treat blood stage malaria. Here, IOE was used to demonstrate its effect on Trypanosoma evansi-infected mice. Methods Analysis of IOE by gas chromatography–mass spectrometry showed the presence of many active components like flavonoids and phenolics. The mice were divided into three groups as follows: vehicle control, T. evansi-infected mice and T. evansi-infected-treated mice. Results The findings demonstrate a significant effect of IOE treatment on T. evansi-infected mice. Parasitemia was decreased by 70%, weight loss was reduced, and splenomegaly was significantly decreased. Additionally, IOE improved the histological architecture of the Spleen, as shown by the improved histological Injury score post-treatment. Anemia was apparent during the course of infection in T. evansi-infected mice; this was reversed upon treatment with IOE to almost the normal level of hemoglobin and erythrocytes. Reduced glutathione and catalase were also ameliorated upon IOE treatment compared to T. evansi-infected mice. Conclusion Overall, this study shows the ameliorative role of IOE against T. evansi-induced Spleen Injury in mice.
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ziziphus spina christi leaf extract pretreatment inhibits liver and Spleen Injury in a mouse model of sepsis via anti oxidant and anti inflammatory effects
2018Co-Authors: Mohamed A. Dkhil, Saleh Alquraishy, Ahmed Abdel E MoneimAbstract:: Sepsis is a systemic response to infection that can result in acute hepatic and splenic damage. Ziziphus spina-christi (L.) is a wild tree used as a medicinal plant by ancient Egyptians. However, little is known about the mechanism underlying its effects on sepsis. The current study investigated the protective effects of a Z. spina-christi leaf extract (ZSCLE) on liver and Spleen damage in a male C57BL/6 mouse model of sepsis, induced by cecal ligation and puncture (CLP). Prior to CLP, ZSCLE was administered daily for five consecutive days via oral gavage at doses of 100, 200, or 300 mg/kg. The mice were euthanized 9 h after CLP, and oxidative stress markers were measured (myeloperoxidase, lipid peroxidation, nitric oxide, and reduced glutathione). In addition, we investigated histological changes, anti-oxidant enzyme activities (superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase), cytokine levels, protein expression of nuclear factor-κB and inducible nitric oxide synthase (iNOS), and mRNA levels of mitogen-activated protein kinase (8, 9, and 14), iNOS, tumor necrosis factor-α, and interleukin-1β. Our results indicated that ZSCLE significantly and dose-dependently inhibited sepsis-induced liver and Spleen Injury. These results suggest that ZSCLE could provide a therapeutic agent for sepsis by inducing anti-inflammatory and anti-oxidant effects.
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beneficial effect of punica granatum peel extract on murine malaria induced Spleen Injury
2016Co-Authors: Murad A Mubaraki, Mohamed A. Dkhil, Taghreed A Hafiz, Saleh AlquraishyAbstract:Multiple drug-resistant malaria parasites have been widely detected, which has encouraged research studies focused on discovering alternative therapies. Medicinal plants such as pomegranate, Punica granatum, have been proven to exhibit antiprotozoal effects and therefore, we examined its effects on murine malaria-induced splenic Injury and oxidative stress in this study. Mice were divided into three groups, a vehicle control and two groups that were infected with 106 Plasmodium chabaudi-parasitized red blood cells (RBCs). The third group was gavaged with 100 μL of 300 mg/kg pomegranate peel extract for 6 days. All mice were euthanized on day 6 post-infection. The results revealed the potential antimalarial, antioxidant, and anti-inflammatory effects of pomegranate. Furthermore, pomegranate peel extracts significantly reduced parasitemia and Spleen index of the treated mice compared to the untreated group. Additionally, the Spleen histology score supported the findings by showing better amelioration in the pomegranate-treated mice than in the untreated mice. Concomitantly, the Spleen capsule thickness showed clear evidence of splenomegaly in the untreated mice, as evidenced by the reduced Spleen capsule. However, pomegranate peel extract exhibited a remarkable restorative effect on the Spleen capsules of the treated mice. Moreover, the extract significantly reduced the expression levels of the proinflammatory cytokines interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ as well as inducible nitric oxide synthase (iNOS). Moreover, our study showed that pomegranate extract profoundly affected oxidative stress levels by reducing the oxidant molecules, nitric oxide (NO) and malondialdehyde (MDA). This study showed that pomegranate clearly induced antimalarial activity in the host by attenuating inflammatory and oxidative stress responses. Furthermore, pomegranate enhanced the innate immune responses and, therefore, could serve an alternative therapy to control clinical malaria episodes and may protect against malaria infection.
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antimalarial and antioxidant activities of indigofera oblongifolia on plasmodium chabaudi induced Spleen tissue Injury in mice
2015Co-Authors: Mahmoud Y. Lubbad, Mohamed A. Dkhil, Saleh AlquraishyAbstract:Malaria is still one of the most common infectious diseases and leads to various public health problems worldwide. Medicinal plants are promising sources for identifying novel agents with potential antimalarial activity. This study aimed to investigate the antimalarial and the antioxidant activities of Indigofera oblongifolia on Plasmodium chabaudi-induced Spleen tissue Injury in mice. Mice were divided into five groups. The first group served as a vehicle control; the second, third, fourth, and fifth groups were infected with 1 × 106 P. chabaudi-parasitized erythrocytes. Mice of the last three groups were gavaged with 100 μl of I. oblongifolia leave extract (IOLE) at a dose of 100, 200, and 300 mg IOLE/kg, respectively, once daily for 7 days. IOLE was significantly able to lower the percentage of parasitemia. The most effective dose was the 100 mg IOLE/kg, which could reduce the parasitemia from about 38 to 12 %. The infection induced Spleen Injury. This was evidenced by disorganization of Spleen white and red pulps, appearance of hemozoin granules and parasitized erythrocytes. These changes in Spleen led to the increased histological score. Also, the infection increased the Spleen oxidative damage where the levels of nitrite/nitrate, malondialdehyde, and catalase were significantly altered. All these infection-induced parameters were significantly improved during IOLE treatment. In addition, the mRNA expression of inflammatory cytokines interleukin-1beta, interleukin-6, and tumor necrosis factor-alpha were upregulated after infection with P. chabaudi, whereas IOLE significantly reduced the expression of these genes. Our results indicate that I. oblongifolia leaves extract exhibits a significant antimalarial and antioxidant effects, and protects host Spleen tissue from injuries induced by P. chabaudi.
Ahmed Abdel E Moneim - One of the best experts on this subject based on the ideXlab platform.
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ziziphus spina christi leaf extract pretreatment inhibits liver and Spleen Injury in a mouse model of sepsis via anti oxidant and anti inflammatory effects
2018Co-Authors: Mohamed A. Dkhil, Saleh Alquraishy, Ahmed Abdel E MoneimAbstract:: Sepsis is a systemic response to infection that can result in acute hepatic and splenic damage. Ziziphus spina-christi (L.) is a wild tree used as a medicinal plant by ancient Egyptians. However, little is known about the mechanism underlying its effects on sepsis. The current study investigated the protective effects of a Z. spina-christi leaf extract (ZSCLE) on liver and Spleen damage in a male C57BL/6 mouse model of sepsis, induced by cecal ligation and puncture (CLP). Prior to CLP, ZSCLE was administered daily for five consecutive days via oral gavage at doses of 100, 200, or 300 mg/kg. The mice were euthanized 9 h after CLP, and oxidative stress markers were measured (myeloperoxidase, lipid peroxidation, nitric oxide, and reduced glutathione). In addition, we investigated histological changes, anti-oxidant enzyme activities (superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase), cytokine levels, protein expression of nuclear factor-κB and inducible nitric oxide synthase (iNOS), and mRNA levels of mitogen-activated protein kinase (8, 9, and 14), iNOS, tumor necrosis factor-α, and interleukin-1β. Our results indicated that ZSCLE significantly and dose-dependently inhibited sepsis-induced liver and Spleen Injury. These results suggest that ZSCLE could provide a therapeutic agent for sepsis by inducing anti-inflammatory and anti-oxidant effects.
Murad A Mubaraki - One of the best experts on this subject based on the ideXlab platform.
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indigofera oblongifolia protects against trypanosomiasis induced Spleen Injury
2019Co-Authors: Mohamed A. Dkhil, Taghreed A Hafiz, Felwa A Thagfan, Esam M Alshaebi, Murad A Mubaraki, Mona F Khalil, Rewaida Abdelgaber, Saleh AlquraishyAbstract:Abstract Background Trypanosomiasis is a neglected tropical disease, transmitted by blood-sucking insects and can affect humans and animals, depending on the species of Trypanosoma parasite. Trypanosoma has acquired resistance to the majority of drugs used; hence, alternative medicines are required. Indigofera oblongifolia leaf extract (IOE) has been shown to treat blood stage malaria. Here, IOE was used to demonstrate its effect on Trypanosoma evansi-infected mice. Methods Analysis of IOE by gas chromatography–mass spectrometry showed the presence of many active components like flavonoids and phenolics. The mice were divided into three groups as follows: vehicle control, T. evansi-infected mice and T. evansi-infected-treated mice. Results The findings demonstrate a significant effect of IOE treatment on T. evansi-infected mice. Parasitemia was decreased by 70%, weight loss was reduced, and splenomegaly was significantly decreased. Additionally, IOE improved the histological architecture of the Spleen, as shown by the improved histological Injury score post-treatment. Anemia was apparent during the course of infection in T. evansi-infected mice; this was reversed upon treatment with IOE to almost the normal level of hemoglobin and erythrocytes. Reduced glutathione and catalase were also ameliorated upon IOE treatment compared to T. evansi-infected mice. Conclusion Overall, this study shows the ameliorative role of IOE against T. evansi-induced Spleen Injury in mice.
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beneficial effect of punica granatum peel extract on murine malaria induced Spleen Injury
2016Co-Authors: Murad A Mubaraki, Mohamed A. Dkhil, Taghreed A Hafiz, Saleh AlquraishyAbstract:Multiple drug-resistant malaria parasites have been widely detected, which has encouraged research studies focused on discovering alternative therapies. Medicinal plants such as pomegranate, Punica granatum, have been proven to exhibit antiprotozoal effects and therefore, we examined its effects on murine malaria-induced splenic Injury and oxidative stress in this study. Mice were divided into three groups, a vehicle control and two groups that were infected with 106 Plasmodium chabaudi-parasitized red blood cells (RBCs). The third group was gavaged with 100 μL of 300 mg/kg pomegranate peel extract for 6 days. All mice were euthanized on day 6 post-infection. The results revealed the potential antimalarial, antioxidant, and anti-inflammatory effects of pomegranate. Furthermore, pomegranate peel extracts significantly reduced parasitemia and Spleen index of the treated mice compared to the untreated group. Additionally, the Spleen histology score supported the findings by showing better amelioration in the pomegranate-treated mice than in the untreated mice. Concomitantly, the Spleen capsule thickness showed clear evidence of splenomegaly in the untreated mice, as evidenced by the reduced Spleen capsule. However, pomegranate peel extract exhibited a remarkable restorative effect on the Spleen capsules of the treated mice. Moreover, the extract significantly reduced the expression levels of the proinflammatory cytokines interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ as well as inducible nitric oxide synthase (iNOS). Moreover, our study showed that pomegranate extract profoundly affected oxidative stress levels by reducing the oxidant molecules, nitric oxide (NO) and malondialdehyde (MDA). This study showed that pomegranate clearly induced antimalarial activity in the host by attenuating inflammatory and oxidative stress responses. Furthermore, pomegranate enhanced the innate immune responses and, therefore, could serve an alternative therapy to control clinical malaria episodes and may protect against malaria infection.
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the potential role of ziziphus spina christi leaf extracts against plasmodium berghei induced liver and Spleen Injury
2016Co-Authors: Taghreed A Hafiz, Murad A MubarakiAbstract:Malaria is still taking the lead as one of the deadliest infectious diseases worldwide, appealing continuous research for further understanding the biology and the pathogenesis of this fascinating parasite. Discovering effective alternative drugs, however, remains a challenging task to address today’s enigmatic problem, the emergence of resistant strains. Factually, the presence of potent natural products considered as a basis for traditional medicine. Ziziphus spina-christi (L.) is a common plant in gulf region and other nations, as a part of their traditional medicine. The aim of this study was to evaluate the antiplasmodium and the antioxidant activity of Z. spina-christi leaf extracts (ZLE) against Plasmodium berghei induced liver and Spleen Injury. The study involved three groups; a vehicle control group, infected with 106 P. berghei-parasitized erythrocytes group and ZLE treated-infected mice with 106 P. berghei-parasitized erythrocytes group. The histopathological examination of the liver and Spleen showed sever histological changes. Animals treated with ZLE showed a significant improvement in the histological picture of liver and Spleen. Biochemical analysis revealed that treatment with ZLE resulted in a significant reinstating of the oxidative markers back to normal level. It could be concluded that ZLE have a power protective role against Plasmodium infection.
Keith T Oldham - One of the best experts on this subject based on the ideXlab platform.
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variation in treatment of pediatric Spleen Injury at trauma centers versus nontrauma centers a call for dissemination of american pediatric surgical association benchmarks and guidelines
2006Co-Authors: Steven Stylianos, Natalia Egorova, Karen S Guice, Ray Arons, Keith T OldhamAbstract:Background American Pediatric Surgical Association consensus guidelines for children with blunt Spleen injuries have been defined and validated in children's hospitals, but large administrative data sets indicate that only 10% to 15% of children with blunt Spleen injuries are treated at children's hospitals. We sought to identify the frequency and compare the treatment of children with Spleen Injury in hospitals with and without recognized trauma expertise, with the aim of identifying a meaningful target for dissemination of benchmarks and consensus guidelines. Study design State health departments' administrative data sets from California, Florida, New Jersey, and New York were analyzed for 2000, 2001, and 2002. All children with head Injury or other nonSpleen abdominal injuries requiring surgery were excluded. Injury Severity Scores were determined by ICDMAP-90. Results There were 3,232 patients with blunt Spleen Injury. Trauma centers had a significantly lower rate of operation for both multiply injured patients (15.3% versus 19.3%, p Conclusions These multistate discharge data indicate that treatment of children with blunt Spleen Injury differs significantly when comparing trauma centers and nontrauma centers. Because nearly two-thirds of these children were treated at trauma centers, dissemination of American Pediatric Surgical Association guidelines and benchmarks through state or regional trauma systems may reduce the number of children having operations for splenic Injury.