The Experts below are selected from a list of 7998 Experts worldwide ranked by ideXlab platform
A J Afolayan - One of the best experts on this subject based on the ideXlab platform.
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phenolic content and antioxidant property of the bark extracts of Ziziphus mucronata willd subsp mucronata willd
BMC Complementary and Alternative Medicine, 2011Co-Authors: O O Olajuyigbe, A J AfolayanAbstract:Background Several plants traditionally used in treatment of a variety of infections in South Africa are reported in ethnobotanical surveys. Many of these plants including Ziziphus mucronata subsp. mucronata lack scientific reports to support their medicinal importance.
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in vitro antibacterial activities of the methanol extract of Ziziphus mucronata willd subsp mucronata willd
Journal of Medicinal Plants Research, 2011Co-Authors: O O Olajuyigbe, A J AfolayanAbstract:The antibacterial activity of crude methanolic extract of Ziziphus mucronata Willd. subsp. mucronata Willd. against both Gram-positive and Gram-negative bacteria were investigated using agar and macrobroth dilution methods. The extract had effective activities against both Gram-positive and Gram-negative bacteria. The agar dilution method indicated that the organisms were susceptible at concentrations ranging between 0.5 and 2.5 mg/ml. While the minimum inhibitory concentration (MIC) values ranged between 0.0391 and 0.625 mg/ml, the minimum bactericidal concentration (MBC) values range between 0.3125 and 1.25 mg/ml. Generally, the Gram-negative bacteria were more susceptible to the extract than the Gram-positive bacteria. The MICindex indicated that the extract was bactericidal at higher concentrations and bacteriostatic at lower concentrations. Our study revealed the broad-spectrum potential of the plant as well as established its ethnobotanical relevance in the traditional treatment of diarrhoeae and dysentery. Key words: Ziziphus mucronata, methanol extract, antibacterial assay, macrobroth dilution.
Erxin Shang - One of the best experts on this subject based on the ideXlab platform.
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hydrophilic interaction ultra high performance liquid chromatography coupled with triple quadrupole mass spectrometry for determination of nucleotides nucleosides and nucleobases in Ziziphus plants
Journal of Chromatography A, 2013Co-Authors: Jin-ao Duan, Dawei Qian, Yuping Tang, Yefei Qian, Shulan Su, Hanqing Wang, Dawei Wu, Erxin ShangAbstract:Abstract In this study, a rapid and sensitive analytical method was developed for the determination of 20 nucleobases, nucleosides and nucleotides in Ziziphus plants at trace levels by using hydrophilic interaction ultra-high performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (HILIC–UHPLC-TQ-MS/MS) in multiple-reaction monitoring (MRM) mode. Under the optimized chromatographic conditions, good separation for 20 target compounds were obtained on a UHPLC Amide column with sub-2 μm particles within 10 min. The overall LODs and LOQs were between 0.11–3.12 ng mL −1 and 0.29–12.48 ng mL −1 for the 20 analytes, respectively. It is the first report about simultaneous analysis of nucleobases, nucleosides and nucleotides in medicinal plants using HILIC–UHPLC-TQ-MS/MS method, which affords good linearity, precision, repeatability and accuracy. The developed method was successfully applied to Ziziphus plant ( Z. jujuba , Z. jujuba var. spinosa and Z. mauritiana ) samples. The analysis showed that the fruits and leaves of Ziziphus plants are rich in nucleosides and nucleobases as well as nucleotides, and could be selected as the healthy food resources. Our results in present study suggest that HILIC–UHPLC-TQ-MS/MS method could be employed as a useful tool for quality assessment of the samples from the Ziziphus plants as well as other medicinal plants or food samples using nucleotides, nucleosides and nucleobases as markers.
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simultaneous qualitative and quantitative analysis of triterpenic acids saponins and flavonoids in the leaves of two Ziziphus species by hplc pda ms elsd
Journal of Pharmaceutical and Biomedical Analysis, 2011Co-Authors: Jin-ao Duan, Jinlong Zhao, Dawei Qian, Yuping Tang, Yefei Qian, Shulan Su, Erxin ShangAbstract:Abstract The leaves of Ziziphus jujuba and Z. jujuba var. spinosa have been utilized as crude drugs for their health benefits in China for thousands of years. To control their quality, a reliable method based on high-performance liquid chromatography coupled with photo diode array and electrospray ionization tandem mass spectrometry detection (HPLC–PDA–ESI-MS/MS) was developed for exploration of the chemical profiles of these jujube leaves. As the results, fourteen constituents including three flavonoids, two saponins and nine triterpenic acids were identified or tentatively characterized. Then, twelve of them such as quercetin-3- O -rutinoside, zizyphus saponins I and II, ceanothic acid, alphitolic acid, maslinic acid, 2 α -hydroxyursolic acid, zizyberanalic acid, epiceanothic acid, ceanothenic acid, betulinic acid, and oleanolic acid were selected as the chemical markers and were determined using an HPLC coupled with evaporative light scattering detection (ELSD) method. The separation was carried out on a Waters Sunfire C 18 column with 0.2% acetic acid and acetonitrile as the mobile phase under gradient elution. The operating conditions of ELSD were set as 80 °C for drift tube temperature and 2.7 l/min for nitrogen flow rate. The developed method was fully validated in terms of linearity, sensitivity, precision, repeatability as well as recovery, and subsequently applied to evaluate the quality of eight batches of Z. jujuba and Z. jujuba var. spinosa leaves from different collections.
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characterization of triterpenic acids in fruits of Ziziphus species by hplc elsd ms
Journal of Agricultural and Food Chemistry, 2010Co-Authors: Jin-ao Duan, Dawei Qian, Yuping Tang, Shulan Su, Nianyun Yang, Erxin ShangAbstract:The fruits of Ziziphus species have been utilized as food as well as crude drugs for their health benefits in China for thousands of years. This paper reported a reversed-phase high-performance liquid chromatography (HPLC) method for the simultaneous characterization and quantitation of 11 triterpenic acids in chloroform extracts of jujube fruits by using an evaporative light scattering detector (ELSD) and electrospray ionization−mass spectrometry (ESI-MS). The results showed that the contents of triterpenic acids in the fruits of Ziziphus jujuba var. spinosa were higher than those in the fruits of Z. jujuba, especially for the compound pomonic acid. Differences were also found among the different parts of Z. jujuba var. spinosa fruits with the sarcocarp having a higher amount of triterpenic acids than the seed and hard core.
Jin-ao Duan - One of the best experts on this subject based on the ideXlab platform.
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Chemical constituents from seeds of Ziziphus mauritiana
Journal of Chinese medicinal materials, 2014Co-Authors: Jin-ao Duan, Jinlong Zhao, Dawei Qian, Wen-jie ZhangAbstract:OBJECTIVE: To study the chemical constituents in the seeds of Ziziphus mauritiana. METHODS: The constituents were isolated by silica column chromatography and their structures were elucidated by physico-chemical properties and spectroscopic analysis. RESULTS: Twelve compounds were isolated from the seeds of Ziziphus mauritiana and identified as betulinic aldehyde (1), betulinic acid (2), ceanothic acid (3), frangufoline (4), spinosin (5), beta-sitosterol (6), daucosterol (7), daucosterol-6'-octadecanoate (8), sucrose (9), docosanoic acid (10), stearic acid (11), palmitoleic acid (12). CONCLUSION: All the compounds are obtained from Ziziphus mauritiana seeds for the first time and compounds 4,5 and 8 are isolated from this plant for the first time.
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hydrophilic interaction ultra high performance liquid chromatography coupled with triple quadrupole mass spectrometry for determination of nucleotides nucleosides and nucleobases in Ziziphus plants
Journal of Chromatography A, 2013Co-Authors: Jin-ao Duan, Dawei Qian, Yuping Tang, Yefei Qian, Shulan Su, Hanqing Wang, Dawei Wu, Erxin ShangAbstract:Abstract In this study, a rapid and sensitive analytical method was developed for the determination of 20 nucleobases, nucleosides and nucleotides in Ziziphus plants at trace levels by using hydrophilic interaction ultra-high performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (HILIC–UHPLC-TQ-MS/MS) in multiple-reaction monitoring (MRM) mode. Under the optimized chromatographic conditions, good separation for 20 target compounds were obtained on a UHPLC Amide column with sub-2 μm particles within 10 min. The overall LODs and LOQs were between 0.11–3.12 ng mL −1 and 0.29–12.48 ng mL −1 for the 20 analytes, respectively. It is the first report about simultaneous analysis of nucleobases, nucleosides and nucleotides in medicinal plants using HILIC–UHPLC-TQ-MS/MS method, which affords good linearity, precision, repeatability and accuracy. The developed method was successfully applied to Ziziphus plant ( Z. jujuba , Z. jujuba var. spinosa and Z. mauritiana ) samples. The analysis showed that the fruits and leaves of Ziziphus plants are rich in nucleosides and nucleobases as well as nucleotides, and could be selected as the healthy food resources. Our results in present study suggest that HILIC–UHPLC-TQ-MS/MS method could be employed as a useful tool for quality assessment of the samples from the Ziziphus plants as well as other medicinal plants or food samples using nucleotides, nucleosides and nucleobases as markers.
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simultaneous qualitative and quantitative analysis of triterpenic acids saponins and flavonoids in the leaves of two Ziziphus species by hplc pda ms elsd
Journal of Pharmaceutical and Biomedical Analysis, 2011Co-Authors: Jin-ao Duan, Jinlong Zhao, Dawei Qian, Yuping Tang, Yefei Qian, Shulan Su, Erxin ShangAbstract:Abstract The leaves of Ziziphus jujuba and Z. jujuba var. spinosa have been utilized as crude drugs for their health benefits in China for thousands of years. To control their quality, a reliable method based on high-performance liquid chromatography coupled with photo diode array and electrospray ionization tandem mass spectrometry detection (HPLC–PDA–ESI-MS/MS) was developed for exploration of the chemical profiles of these jujube leaves. As the results, fourteen constituents including three flavonoids, two saponins and nine triterpenic acids were identified or tentatively characterized. Then, twelve of them such as quercetin-3- O -rutinoside, zizyphus saponins I and II, ceanothic acid, alphitolic acid, maslinic acid, 2 α -hydroxyursolic acid, zizyberanalic acid, epiceanothic acid, ceanothenic acid, betulinic acid, and oleanolic acid were selected as the chemical markers and were determined using an HPLC coupled with evaporative light scattering detection (ELSD) method. The separation was carried out on a Waters Sunfire C 18 column with 0.2% acetic acid and acetonitrile as the mobile phase under gradient elution. The operating conditions of ELSD were set as 80 °C for drift tube temperature and 2.7 l/min for nitrogen flow rate. The developed method was fully validated in terms of linearity, sensitivity, precision, repeatability as well as recovery, and subsequently applied to evaluate the quality of eight batches of Z. jujuba and Z. jujuba var. spinosa leaves from different collections.
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characterization of triterpenic acids in fruits of Ziziphus species by hplc elsd ms
Journal of Agricultural and Food Chemistry, 2010Co-Authors: Jin-ao Duan, Dawei Qian, Yuping Tang, Shulan Su, Nianyun Yang, Erxin ShangAbstract:The fruits of Ziziphus species have been utilized as food as well as crude drugs for their health benefits in China for thousands of years. This paper reported a reversed-phase high-performance liquid chromatography (HPLC) method for the simultaneous characterization and quantitation of 11 triterpenic acids in chloroform extracts of jujube fruits by using an evaporative light scattering detector (ELSD) and electrospray ionization−mass spectrometry (ESI-MS). The results showed that the contents of triterpenic acids in the fruits of Ziziphus jujuba var. spinosa were higher than those in the fruits of Z. jujuba, especially for the compound pomonic acid. Differences were also found among the different parts of Z. jujuba var. spinosa fruits with the sarcocarp having a higher amount of triterpenic acids than the seed and hard core.
O O Olajuyigbe - One of the best experts on this subject based on the ideXlab platform.
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phenolic content and antioxidant property of the bark extracts of Ziziphus mucronata willd subsp mucronata willd
BMC Complementary and Alternative Medicine, 2011Co-Authors: O O Olajuyigbe, A J AfolayanAbstract:Background Several plants traditionally used in treatment of a variety of infections in South Africa are reported in ethnobotanical surveys. Many of these plants including Ziziphus mucronata subsp. mucronata lack scientific reports to support their medicinal importance.
-
in vitro antibacterial activities of the methanol extract of Ziziphus mucronata willd subsp mucronata willd
Journal of Medicinal Plants Research, 2011Co-Authors: O O Olajuyigbe, A J AfolayanAbstract:The antibacterial activity of crude methanolic extract of Ziziphus mucronata Willd. subsp. mucronata Willd. against both Gram-positive and Gram-negative bacteria were investigated using agar and macrobroth dilution methods. The extract had effective activities against both Gram-positive and Gram-negative bacteria. The agar dilution method indicated that the organisms were susceptible at concentrations ranging between 0.5 and 2.5 mg/ml. While the minimum inhibitory concentration (MIC) values ranged between 0.0391 and 0.625 mg/ml, the minimum bactericidal concentration (MBC) values range between 0.3125 and 1.25 mg/ml. Generally, the Gram-negative bacteria were more susceptible to the extract than the Gram-positive bacteria. The MICindex indicated that the extract was bactericidal at higher concentrations and bacteriostatic at lower concentrations. Our study revealed the broad-spectrum potential of the plant as well as established its ethnobotanical relevance in the traditional treatment of diarrhoeae and dysentery. Key words: Ziziphus mucronata, methanol extract, antibacterial assay, macrobroth dilution.
Dawei Qian - One of the best experts on this subject based on the ideXlab platform.
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Chemical constituents from seeds of Ziziphus mauritiana
Journal of Chinese medicinal materials, 2014Co-Authors: Jin-ao Duan, Jinlong Zhao, Dawei Qian, Wen-jie ZhangAbstract:OBJECTIVE: To study the chemical constituents in the seeds of Ziziphus mauritiana. METHODS: The constituents were isolated by silica column chromatography and their structures were elucidated by physico-chemical properties and spectroscopic analysis. RESULTS: Twelve compounds were isolated from the seeds of Ziziphus mauritiana and identified as betulinic aldehyde (1), betulinic acid (2), ceanothic acid (3), frangufoline (4), spinosin (5), beta-sitosterol (6), daucosterol (7), daucosterol-6'-octadecanoate (8), sucrose (9), docosanoic acid (10), stearic acid (11), palmitoleic acid (12). CONCLUSION: All the compounds are obtained from Ziziphus mauritiana seeds for the first time and compounds 4,5 and 8 are isolated from this plant for the first time.
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hydrophilic interaction ultra high performance liquid chromatography coupled with triple quadrupole mass spectrometry for determination of nucleotides nucleosides and nucleobases in Ziziphus plants
Journal of Chromatography A, 2013Co-Authors: Jin-ao Duan, Dawei Qian, Yuping Tang, Yefei Qian, Shulan Su, Hanqing Wang, Dawei Wu, Erxin ShangAbstract:Abstract In this study, a rapid and sensitive analytical method was developed for the determination of 20 nucleobases, nucleosides and nucleotides in Ziziphus plants at trace levels by using hydrophilic interaction ultra-high performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (HILIC–UHPLC-TQ-MS/MS) in multiple-reaction monitoring (MRM) mode. Under the optimized chromatographic conditions, good separation for 20 target compounds were obtained on a UHPLC Amide column with sub-2 μm particles within 10 min. The overall LODs and LOQs were between 0.11–3.12 ng mL −1 and 0.29–12.48 ng mL −1 for the 20 analytes, respectively. It is the first report about simultaneous analysis of nucleobases, nucleosides and nucleotides in medicinal plants using HILIC–UHPLC-TQ-MS/MS method, which affords good linearity, precision, repeatability and accuracy. The developed method was successfully applied to Ziziphus plant ( Z. jujuba , Z. jujuba var. spinosa and Z. mauritiana ) samples. The analysis showed that the fruits and leaves of Ziziphus plants are rich in nucleosides and nucleobases as well as nucleotides, and could be selected as the healthy food resources. Our results in present study suggest that HILIC–UHPLC-TQ-MS/MS method could be employed as a useful tool for quality assessment of the samples from the Ziziphus plants as well as other medicinal plants or food samples using nucleotides, nucleosides and nucleobases as markers.
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simultaneous qualitative and quantitative analysis of triterpenic acids saponins and flavonoids in the leaves of two Ziziphus species by hplc pda ms elsd
Journal of Pharmaceutical and Biomedical Analysis, 2011Co-Authors: Jin-ao Duan, Jinlong Zhao, Dawei Qian, Yuping Tang, Yefei Qian, Shulan Su, Erxin ShangAbstract:Abstract The leaves of Ziziphus jujuba and Z. jujuba var. spinosa have been utilized as crude drugs for their health benefits in China for thousands of years. To control their quality, a reliable method based on high-performance liquid chromatography coupled with photo diode array and electrospray ionization tandem mass spectrometry detection (HPLC–PDA–ESI-MS/MS) was developed for exploration of the chemical profiles of these jujube leaves. As the results, fourteen constituents including three flavonoids, two saponins and nine triterpenic acids were identified or tentatively characterized. Then, twelve of them such as quercetin-3- O -rutinoside, zizyphus saponins I and II, ceanothic acid, alphitolic acid, maslinic acid, 2 α -hydroxyursolic acid, zizyberanalic acid, epiceanothic acid, ceanothenic acid, betulinic acid, and oleanolic acid were selected as the chemical markers and were determined using an HPLC coupled with evaporative light scattering detection (ELSD) method. The separation was carried out on a Waters Sunfire C 18 column with 0.2% acetic acid and acetonitrile as the mobile phase under gradient elution. The operating conditions of ELSD were set as 80 °C for drift tube temperature and 2.7 l/min for nitrogen flow rate. The developed method was fully validated in terms of linearity, sensitivity, precision, repeatability as well as recovery, and subsequently applied to evaluate the quality of eight batches of Z. jujuba and Z. jujuba var. spinosa leaves from different collections.
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characterization of triterpenic acids in fruits of Ziziphus species by hplc elsd ms
Journal of Agricultural and Food Chemistry, 2010Co-Authors: Jin-ao Duan, Dawei Qian, Yuping Tang, Shulan Su, Nianyun Yang, Erxin ShangAbstract:The fruits of Ziziphus species have been utilized as food as well as crude drugs for their health benefits in China for thousands of years. This paper reported a reversed-phase high-performance liquid chromatography (HPLC) method for the simultaneous characterization and quantitation of 11 triterpenic acids in chloroform extracts of jujube fruits by using an evaporative light scattering detector (ELSD) and electrospray ionization−mass spectrometry (ESI-MS). The results showed that the contents of triterpenic acids in the fruits of Ziziphus jujuba var. spinosa were higher than those in the fruits of Z. jujuba, especially for the compound pomonic acid. Differences were also found among the different parts of Z. jujuba var. spinosa fruits with the sarcocarp having a higher amount of triterpenic acids than the seed and hard core.
