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Pan Ruha - One of the best experts on this subject based on the ideXlab platform.

  • Isolation and identification of ginger Fusarium wilt pathogen and the effect of Spore Suspension concentration on the extent of disease
    Journal of Nanjing Agricultural University, 2014
    Co-Authors: Pan Ruha
    Abstract:

    A pathogenetic fungus strain was isolated from the diseased ginger in Pingdu,Shandong Province,which was identified as Fusarium oxysporum f. sp. zingiberi by morphological identification,rDNA-ITS sequence analysis and inoculation experiment. Spores of the strain were inoculated into healthy soils in pot at different concentration levels to study the effect of different inoculation Spore Suspension of pathogen on ginger Fusarium wilt. Results showed a close relationship between the concentration of inocula in soil and the disease incidence rate of ginger Fusarium wilt. The minimal concentration that induced the ginger Fusarium wilt was 103per gram soil,and the disease incidence increased with the increase of inocula concentration in soil. Until the inocula concentration attained a certain level(105),the disease incidence would no longer increase. The Spore Suspension concentration inoculated was found to have a significant influence on the abundance of pathogen in rhizosphere and bulk soil of the ginger plants. The abundance of pathogen in rhizosphere soil was 1. 19 to 1. 86 times higher than those in bulk soil in different treatments. It was concluded that whether the ginger Fusarium wilt occurred or not was related to the abundance of pathogen in both rhizosphere and bulk soil,while the disease incidence was mainly determined by the number of the pathogen in plant rhizosphere soil.

Thomas F Cummings - One of the best experts on this subject based on the ideXlab platform.

  • a plant stem inoculation assay for assessing transmission of phytophthora infestans from potato seed tubers to emerged shoots
    Plant Disease, 2013
    Co-Authors: Dennis A Johnson, Thomas F Cummings
    Abstract:

    A stem inoculation assay was developed to assess transmission of Phytophthora infestans from the base of belowground potato stems to emerged aboveground shoots. Sporangia of P. infestans subsequently developed on aboveground stems after belowground stems were inoculated with isolates BF05 (US 8 genotype), WA10.1 (US 24), and 110B (US 11) by (i) placing a filter paper square saturated with a Spore Suspension onto nonwounded belowground stem tissue, (ii) placing a filter paper square saturated with a Spore Suspension onto wounded belowground stem tissue, and (iii) dipping the base of the cut belowground stem into a Spore Suspension. Plant stems were then incubated in a humidity chamber at 10 to 21.5°C for 7 to 28 days. Incidence of aboveground stems with sporulation was significantly less when stems were inoculated with isolate 110B than with isolates BF05 and WA10.1 but length of latent period did not differ among the three isolates. Incidence of aboveground stems with sporangia was significantly (P < 0.05) greater for 'Russet Norkotah' than for 'Umatilla Russet' when inoculated near the point of seed piece attachment. Incidence of aboveground stems with sporulation did not differ among incubation temperatures of 10, 15, and 21.5°C. Latent period significantly increased as temperature decreased. Age of stem did not appear to affect incidence of transmission. Incidence of transmission of P. infestans to aboveground stems from the base of belowground stems was significantly lowered when aboveground stems were treated with selected fungicides 1 h before inoculation.

Dennis A Johnson - One of the best experts on this subject based on the ideXlab platform.

  • a plant stem inoculation assay for assessing transmission of phytophthora infestans from potato seed tubers to emerged shoots
    Plant Disease, 2013
    Co-Authors: Dennis A Johnson, Thomas F Cummings
    Abstract:

    A stem inoculation assay was developed to assess transmission of Phytophthora infestans from the base of belowground potato stems to emerged aboveground shoots. Sporangia of P. infestans subsequently developed on aboveground stems after belowground stems were inoculated with isolates BF05 (US 8 genotype), WA10.1 (US 24), and 110B (US 11) by (i) placing a filter paper square saturated with a Spore Suspension onto nonwounded belowground stem tissue, (ii) placing a filter paper square saturated with a Spore Suspension onto wounded belowground stem tissue, and (iii) dipping the base of the cut belowground stem into a Spore Suspension. Plant stems were then incubated in a humidity chamber at 10 to 21.5°C for 7 to 28 days. Incidence of aboveground stems with sporulation was significantly less when stems were inoculated with isolate 110B than with isolates BF05 and WA10.1 but length of latent period did not differ among the three isolates. Incidence of aboveground stems with sporangia was significantly (P < 0.05) greater for 'Russet Norkotah' than for 'Umatilla Russet' when inoculated near the point of seed piece attachment. Incidence of aboveground stems with sporulation did not differ among incubation temperatures of 10, 15, and 21.5°C. Latent period significantly increased as temperature decreased. Age of stem did not appear to affect incidence of transmission. Incidence of transmission of P. infestans to aboveground stems from the base of belowground stems was significantly lowered when aboveground stems were treated with selected fungicides 1 h before inoculation.

Viviane Zahner - One of the best experts on this subject based on the ideXlab platform.

A. Adebanjo - One of the best experts on this subject based on the ideXlab platform.

  • Biocontrol of brown blotch of cowpea caused by Colletotrichum truncatum with Trichoderma viride
    Crop Protection, 1996
    Co-Authors: S. A. Bankole, A. Adebanjo
    Abstract:

    Trichoderma viride isolated from cowpea phylloplane hyperasitised the mycelium of Colletotrichum truncatum, causal agent of brown blotch disease of cowpea in vitro. T. viride treatment in the form of a seed dip in a Spore Suspension (IO’ conidia/ml) and soil drenching with a Spore Suspension were very effective in reducing infection from brown blotch infected seeds. Seed treatment with dry Spores of the antagonist did not significantly reduce disease incidence compared to the control. T. viride as a Spore Suspension foliar spray applied once or twice weekly from three days after inoculation of cowpea seedlings with C. truncatum, significantly reduced brown blotch incidence in the field. These two treatments increased grain yield compared with inoculated unprotected plants. The yield from plots sprayed twice weekly with the antagonist was statistically comparable with inoculated plants sprayed weekly with benomyl. Copyright @ 1996 Elsevier Science Ltd