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Norhayati Moktar - One of the best experts on this subject based on the ideXlab platform.

  • wintergreen oil a novel method in wheatley s trichrome Staining Technique
    Journal of Microbiological Methods, 2012
    Co-Authors: Fatmah Md Salleh, Tengku Shahrul Anuar, Azlin Mohd Yasin, Norhayati Moktar
    Abstract:

    Permanent Staining of faecal smears by Wheatley's trichrome Technique has been used by many scientists for the detection of parasites in the past and it was found to be highly sensitive. This study was conducted to evaluate the use of Wintergreen oil in comparison with xylene in Wheatley's trichrome Staining Technique, as the reference Technique. In a blind comparison study, 500 collected faecal samples from aboriginal communities were examined. Wintergreen oil was found to be more superior than xylene as a clearing agent in the Wheatley's trichrome Staining of polyvinyl alcohol-fixed faecal smears for the identification of intestinal protozoa. Elimination of toxic, carcinogenic, and fire hazards makes Wintergreen oil the preferred choice in routine parasitology examinations.

  • wintergreen oil a novel method in wheatley s trichrome Staining Technique
    Journal of Microbiological Methods, 2012
    Co-Authors: Fatmah Md Salleh, Tengku Shahrul Anuar, Azlin Mohd Yasin, Norhayati Moktar
    Abstract:

    Permanent Staining of faecal smears by Wheatley's trichrome Technique has been used by many scientists for the detection of parasites in the past and it was found to be highly sensitive. This study was conducted to evaluate the use of Wintergreen oil in comparison with xylene in Wheatley's trichrome Staining Technique, as the reference Technique. In a blind comparison study, 500 collected faecal samples from aboriginal communities were examined. Wintergreen oil was found to be more superior than xylene as a clearing agent in the Wheatley's trichrome Staining of polyvinyl alcohol-fixed faecal smears for the identification of intestinal protozoa. Elimination of toxic, carcinogenic, and fire hazards makes Wintergreen oil the preferred choice in routine parasitology examinations.

Fatmah Md Salleh - One of the best experts on this subject based on the ideXlab platform.

  • wintergreen oil a novel method in wheatley s trichrome Staining Technique
    Journal of Microbiological Methods, 2012
    Co-Authors: Fatmah Md Salleh, Tengku Shahrul Anuar, Azlin Mohd Yasin, Norhayati Moktar
    Abstract:

    Permanent Staining of faecal smears by Wheatley's trichrome Technique has been used by many scientists for the detection of parasites in the past and it was found to be highly sensitive. This study was conducted to evaluate the use of Wintergreen oil in comparison with xylene in Wheatley's trichrome Staining Technique, as the reference Technique. In a blind comparison study, 500 collected faecal samples from aboriginal communities were examined. Wintergreen oil was found to be more superior than xylene as a clearing agent in the Wheatley's trichrome Staining of polyvinyl alcohol-fixed faecal smears for the identification of intestinal protozoa. Elimination of toxic, carcinogenic, and fire hazards makes Wintergreen oil the preferred choice in routine parasitology examinations.

  • wintergreen oil a novel method in wheatley s trichrome Staining Technique
    Journal of Microbiological Methods, 2012
    Co-Authors: Fatmah Md Salleh, Tengku Shahrul Anuar, Azlin Mohd Yasin, Norhayati Moktar
    Abstract:

    Permanent Staining of faecal smears by Wheatley's trichrome Technique has been used by many scientists for the detection of parasites in the past and it was found to be highly sensitive. This study was conducted to evaluate the use of Wintergreen oil in comparison with xylene in Wheatley's trichrome Staining Technique, as the reference Technique. In a blind comparison study, 500 collected faecal samples from aboriginal communities were examined. Wintergreen oil was found to be more superior than xylene as a clearing agent in the Wheatley's trichrome Staining of polyvinyl alcohol-fixed faecal smears for the identification of intestinal protozoa. Elimination of toxic, carcinogenic, and fire hazards makes Wintergreen oil the preferred choice in routine parasitology examinations.

John N Saddler - One of the best experts on this subject based on the ideXlab platform.

  • use of the simons Staining Technique to assess cellulose accessibility in pretreated substrates
    Industrial Biotechnology, 2012
    Co-Authors: Richard P Chandra, John N Saddler
    Abstract:

    Abstract The specificity of the Simons' Staining Technique for determining the specific surface area (SSA) of cellulose—the amount of cellulose in a pretreated substrate that is accessible to cellulases—was assessed over a range of cellulosic substrates. Both the Direct Blue (DB) dye and the high molecular weight (HMW) fraction (>100 kda) of the Direct Orange (DO) dye were shown to bind with high specificity to the cellulose rather than hemicellulose and/or lignin. The use of the DO dye by itself could also indicate differences in the accessibility/SSA of various types of cellulose resulting in an R2 value of 0.87 when plotted against enzymatic hydrolysis yields. The previously reported fluctuations in the ratio of DO:DB when the dyes were adsorbed to similar cellulosic substrates were shown to be primarily due to differences in the method used for the isolation of the high molecular weight fraction of the DO dye. A procedure for the enrichment of the >100 kDa HMW fraction of the DO using a minimum of two...

  • the characterization of pretreated lignocellulosic substrates prior to enzymatic hydrolysis part 1 a modified simons Staining Technique
    Biotechnology Progress, 2008
    Co-Authors: Richard P Chandra, Shannon Ewanick, Carmen Hsieh, John N Saddler
    Abstract:

    To date, there is limited knowledge available regarding the key features of pretreated lignocellulosic substrates that promote the effective enzymatic hydrolysis of the cellulose component to glucose during bioconversion processes to produce ethanol. Fundamentally, cellulase enzymes require access to the cellulose to carry out effective hydrolysis. Porosity and the overall surface area of substrates have major structural features influencing the hydrolysis of pretreated substrates by cellulases. Simons' Stain (SS) is a potentially useful semiquantitative method for estimating the available surface area of lignocellulosic substrates. In this study, a modified, rapid SS method was developed, where the processing time was decreased from >50 to 6 h and the maximum dye adsorbed on the substrate was calculated using the adsorption isotherm for the orange and blue components of the dye mixture. The modified SS test readily measures the decrease in accessibility and hydrolyzability of a steam pretreated substrate that had been dried under three different drying regimes. For each of the lignocellulosic substrates, the total dye adsorption correlated well with the hydrolysis yields resulting in a correlation coefficient of r(2) = 0.95. The modified SS procedure is an effective tool for assessing how lignocellulosic substrates might be potentially hydrolyzed by cellulases.

Massimo Fiorilli - One of the best experts on this subject based on the ideXlab platform.

  • flow acetone Staining Technique a highly efficient procedure for the simultaneous analysis of dna content cell morphology and immunophenotype by flow cytometry
    Cytometry Part A, 2008
    Co-Authors: Maurizio Carbonari, Debora Mancaniello, Tiziana Tedesco, Massimo Fiorilli
    Abstract:

    The accurate determination of cell cycle, immunophenotypes and morphology at single-cell level is not fully achieved by current flow cytometry protocols. Acetone, a coagulant fixative/permealizing agent, is widely used in static cytometry, but is impractical in flow cytometry because of its shrinking effect. We sought for conditions of acetone treatment that could permit the simultaneous analysis of physical parameters, surface and intracellular immunoStaining, and DNA content. We evaluated different experimental conditions (concentration, duration of fixation, temperature, presence of proteins) to test the capacity of acetone fixation/permeabilization to preserve cell physical parameters (forward and side scatters, FSC, and SSC) and immunophenotyping while allowing stoichiometric DNA Staining. The commonly used ethanol fixation Technique was used as reference method. To detect phenotypes and DNA content simultaneously, we employed 7-aminoactinomycin D (7-AAD) as "intercalating" dye for DNA in spite of, or just for, its controversial ability in stoichiometric DNA Staining. Cells were resting peripheral blood monucleated cells (PBMCs), T- and B-cell blasts obtained by PBMCs stimulation, and the human cell lines Ramos and Shep. Acetone fixation, preserving both the recovery and the physical parameters of cells, is drastically influenced by temperature of treatment and is practicable only when the protocol is realized at 8 degrees C. Under this condition, acetone maintains the immunophenotypic fluorescences (realized before or after the fixation) better than ethanol. Stoichiometric DNA Staining of acetone processed cells, the variation coefficients (CV) of frequency distributions of G1/G0 and G2/M phases, the modes ratio of these distributions and doublets generation are at least comparable to those obtained with ethanol treatment. The assay developed in the present study, that we called flow acetone-Staining Technique (FAST), accurately analyzes cell cycle, physical parameters and immunophenotypes in heterogenous cell populations, and thus provides a useful tool for cytomics.

  • Flow acetone-Staining Technique: A highly efficient procedure for the simultaneous analysis of dna content, cell morphology, and immunophenotype by flow cytometry
    'Wiley', 2008
    Co-Authors: Maurizio Carbonari, Debora Mancaniello, Tiziana Tedesco, Massimo Fiorilli
    Abstract:

    The accurate determination of cell cycle, immunophenotypes and morphology at single-cell level is not fully achieved by current flow cytometry protocols. Acetone, a coagulant fixative/permealizing agent, is widely used in static cytometry, but is impractical in flow cytometry because of its shrinking effect. We sought for conditions of acetone treatment that could permit the simultaneous analysis of physical parameters, surface and intracellular immunoStaining, and DNA content. We evaluated different experimental conditions (concentration, duration of fixation, temperature, presence of proteins) to test the capacity of acetone fixation/permeabilization to preserve cell physical parameters (forward and side scatters, FSC, and SSC) and immunophenotyping while allowing stoichiometric DNA Staining. The commonly used ethanol fixation Technique was used as reference method. To detect phenotypes and DNA content simultaneously, we employed 7-aminoactinomycin D (7-AAD) as " intercalating" dye for DNA in spite of, or just for, its controversial ability in stoichiometric DNA Staining. Cells were resting peripheral blood monucleated cells (PBMCs), T- and B-cell blasts obtained by PBMCs stimulation, and the human cell lines Ramos and Shep. Acetone fixation, preserving both the recovery and the physical parameters of cells, is drastically influenced by temperature of treatment and is practicable only when the protocol is realized at 8°C. Under this condition, acetone maintains the immunophenotypic fluorescences (realized before or after the fixation) better than ethanol. Stoichiometric DNA Staining of acetone processed cells, the variation coefficients (CV) of frequency distributions of G1/G0 and G2/M phases, the modes ratio of these distributions and doublets generation are at least comparable to those obtained with ethanol treatment. The assay developed in the present study, that we called flow acetone-Staining Technique (FAST), accurately analyzes cell cycle, physical parameters and immunophenotypes in heterogenous cell populations, and thus provides a useful tool for cytomics. © 2008 International Society for Analytical Cytology

Tengku Shahrul Anuar - One of the best experts on this subject based on the ideXlab platform.

  • wintergreen oil a novel method in wheatley s trichrome Staining Technique
    Journal of Microbiological Methods, 2012
    Co-Authors: Fatmah Md Salleh, Tengku Shahrul Anuar, Azlin Mohd Yasin, Norhayati Moktar
    Abstract:

    Permanent Staining of faecal smears by Wheatley's trichrome Technique has been used by many scientists for the detection of parasites in the past and it was found to be highly sensitive. This study was conducted to evaluate the use of Wintergreen oil in comparison with xylene in Wheatley's trichrome Staining Technique, as the reference Technique. In a blind comparison study, 500 collected faecal samples from aboriginal communities were examined. Wintergreen oil was found to be more superior than xylene as a clearing agent in the Wheatley's trichrome Staining of polyvinyl alcohol-fixed faecal smears for the identification of intestinal protozoa. Elimination of toxic, carcinogenic, and fire hazards makes Wintergreen oil the preferred choice in routine parasitology examinations.

  • wintergreen oil a novel method in wheatley s trichrome Staining Technique
    Journal of Microbiological Methods, 2012
    Co-Authors: Fatmah Md Salleh, Tengku Shahrul Anuar, Azlin Mohd Yasin, Norhayati Moktar
    Abstract:

    Permanent Staining of faecal smears by Wheatley's trichrome Technique has been used by many scientists for the detection of parasites in the past and it was found to be highly sensitive. This study was conducted to evaluate the use of Wintergreen oil in comparison with xylene in Wheatley's trichrome Staining Technique, as the reference Technique. In a blind comparison study, 500 collected faecal samples from aboriginal communities were examined. Wintergreen oil was found to be more superior than xylene as a clearing agent in the Wheatley's trichrome Staining of polyvinyl alcohol-fixed faecal smears for the identification of intestinal protozoa. Elimination of toxic, carcinogenic, and fire hazards makes Wintergreen oil the preferred choice in routine parasitology examinations.