The Experts below are selected from a list of 114 Experts worldwide ranked by ideXlab platform
Yan Zhang - One of the best experts on this subject based on the ideXlab platform.
-
prevention of dermal abscess formation caused by Staphylococcus aureus using Phage jd007 in nude mice
Frontiers in Microbiology, 2018Co-Authors: Bingyu Ding, Qingtian Li, Ke Dong, Yan Zhang, Li LiAbstract:Aim: In this study, Staphylococcus Phage JD007 bactericidal activity and induced immune responses during treatment were assessed in a dermal abscess model. Materials & methods: Dermal abscesses in nude mice were established by injecting a clinical isolate of S. aureus SA325 isolated from the back under-dermal abscess of an in-patient. Results: Phage JD007 was able to inhibit the growth of S. aureus SA325 at MOI=1 or 10, significantly preventing the formation of dermal abscesses. Moderate immune responses were observed in the prevention group through detection of cytokines. Conclusion: Phage JD007 inhibits the formation of dermal abscesses caused by a clinical S. aureus strain in nude mice without robust immune responses.
-
characterization and complete genome of the virulent myoviridae Phage jd007 active against a variety of Staphylococcus aureus isolates from different hospitals in shanghai china
Virology Journal, 2017Co-Authors: Tingting Feng, Feifei Gu, Qingtian Li, Ke Dong, Yan ZhangAbstract:The implementation of Phage therapy is re-emerging with the increase in widespread antibiotic-resistant bacteria. Staphylococcus Phage JD007 was characterized and its complete genome sequence analysed. Staphylococcus Phage JD007 was classified as belonging to the Myoviridae family based on its morphology, as observed by transmission electron microscopy. Its lytic activity was stable between pH 5–11 and below 42 °C; moreover, an absorbance curve showed that nearly 90% of the viral particles had adsorbed to its host after a 20 min co-incubation. The complete genome size is 141,836 bp, making JD007 one of the largest Staphylococcus Phages of Myoviridae. No identifiable resistance or virulence genes were found in the JD007 genome. JD007 was able to lyse 95% of S. aureus isolates, including the prevalent ST239-MRSA and ST59-MRSA strains isolated from different hospitals in Shanghai, China, and inhibition assays showed that JD007 could inhibit S. aureus growth at a multiplicity of infection of 0.1. The results suggested that Staphylococcus Phage JD007 can potentially be used in Phage therapy or for the detection of S. aureus.
Michel Drancourt - One of the best experts on this subject based on the ideXlab platform.
-
Staphylococcus aureus subsp. anaerobius strain ST1464 genome sequence
Standards in Genomic Sciences, 2013Co-Authors: Haitham Elbir, Catherine Robert, Ti Thien Nguyen, Grégory Gimenez, Sulieman M. Sanousi, Jan-ingmar Flock, Didier Raoult, Michel DrancourtAbstract:Staphylococcus aureus subsp. anaerobius is responsible for Morel’s disease in animals and a cause of abscess in humans. It is characterized by a microaerophilic growth, contrary to the other strains of S. aureus . The 2,604,446-bp genome (32.7% GC content) of S. anaerobius ST1464 comprises one chromosome and no plasmids. The chromosome contains 2,660 open reading frames (ORFs), 49 tRNAs and three complete rRNAs, forming one complete operon. The size of ORFs ranges between 100 to 4,600 bp except for two ORFs of 6,417 and 7,173 bp encoding segregation ATPase and non-ribosomal peptide synthase, respectively. The chromosome harbors Staphylococcus Phage 2638A genome and incomplete Staphylococcus Phage genome PT1028, but no detectable CRISPRS. The antibiotic resistance gene for tetracycline was found although Staphylococcus aureus subsp. anaerobius is susceptible to tetracycline in-vitro . Intact oxygen detoxification genes encode superoxide dismutase and cytochrome quinol oxidase whereas the catalase gene is impaired by a stop codon. Based on the genome, in-silico multilocus sequence typing indicates that S. aureus subsp. anaerobius emerged as a clone separated from all other S. aureus strains, illustrating host-adaptation linked to missing functions. Availability of S. aureus subsp. anaerobius genome could prompt the development of post-genomic tools for its rapid discrimination from S. aureus .
Ke Dong - One of the best experts on this subject based on the ideXlab platform.
-
prevention of dermal abscess formation caused by Staphylococcus aureus using Phage jd007 in nude mice
Frontiers in Microbiology, 2018Co-Authors: Bingyu Ding, Qingtian Li, Ke Dong, Yan Zhang, Li LiAbstract:Aim: In this study, Staphylococcus Phage JD007 bactericidal activity and induced immune responses during treatment were assessed in a dermal abscess model. Materials & methods: Dermal abscesses in nude mice were established by injecting a clinical isolate of S. aureus SA325 isolated from the back under-dermal abscess of an in-patient. Results: Phage JD007 was able to inhibit the growth of S. aureus SA325 at MOI=1 or 10, significantly preventing the formation of dermal abscesses. Moderate immune responses were observed in the prevention group through detection of cytokines. Conclusion: Phage JD007 inhibits the formation of dermal abscesses caused by a clinical S. aureus strain in nude mice without robust immune responses.
-
characterization and complete genome of the virulent myoviridae Phage jd007 active against a variety of Staphylococcus aureus isolates from different hospitals in shanghai china
Virology Journal, 2017Co-Authors: Tingting Feng, Feifei Gu, Qingtian Li, Ke Dong, Yan ZhangAbstract:The implementation of Phage therapy is re-emerging with the increase in widespread antibiotic-resistant bacteria. Staphylococcus Phage JD007 was characterized and its complete genome sequence analysed. Staphylococcus Phage JD007 was classified as belonging to the Myoviridae family based on its morphology, as observed by transmission electron microscopy. Its lytic activity was stable between pH 5–11 and below 42 °C; moreover, an absorbance curve showed that nearly 90% of the viral particles had adsorbed to its host after a 20 min co-incubation. The complete genome size is 141,836 bp, making JD007 one of the largest Staphylococcus Phages of Myoviridae. No identifiable resistance or virulence genes were found in the JD007 genome. JD007 was able to lyse 95% of S. aureus isolates, including the prevalent ST239-MRSA and ST59-MRSA strains isolated from different hospitals in Shanghai, China, and inhibition assays showed that JD007 could inhibit S. aureus growth at a multiplicity of infection of 0.1. The results suggested that Staphylococcus Phage JD007 can potentially be used in Phage therapy or for the detection of S. aureus.
Qingtian Li - One of the best experts on this subject based on the ideXlab platform.
-
prevention of dermal abscess formation caused by Staphylococcus aureus using Phage jd007 in nude mice
Frontiers in Microbiology, 2018Co-Authors: Bingyu Ding, Qingtian Li, Ke Dong, Yan Zhang, Li LiAbstract:Aim: In this study, Staphylococcus Phage JD007 bactericidal activity and induced immune responses during treatment were assessed in a dermal abscess model. Materials & methods: Dermal abscesses in nude mice were established by injecting a clinical isolate of S. aureus SA325 isolated from the back under-dermal abscess of an in-patient. Results: Phage JD007 was able to inhibit the growth of S. aureus SA325 at MOI=1 or 10, significantly preventing the formation of dermal abscesses. Moderate immune responses were observed in the prevention group through detection of cytokines. Conclusion: Phage JD007 inhibits the formation of dermal abscesses caused by a clinical S. aureus strain in nude mice without robust immune responses.
-
characterization and complete genome of the virulent myoviridae Phage jd007 active against a variety of Staphylococcus aureus isolates from different hospitals in shanghai china
Virology Journal, 2017Co-Authors: Tingting Feng, Feifei Gu, Qingtian Li, Ke Dong, Yan ZhangAbstract:The implementation of Phage therapy is re-emerging with the increase in widespread antibiotic-resistant bacteria. Staphylococcus Phage JD007 was characterized and its complete genome sequence analysed. Staphylococcus Phage JD007 was classified as belonging to the Myoviridae family based on its morphology, as observed by transmission electron microscopy. Its lytic activity was stable between pH 5–11 and below 42 °C; moreover, an absorbance curve showed that nearly 90% of the viral particles had adsorbed to its host after a 20 min co-incubation. The complete genome size is 141,836 bp, making JD007 one of the largest Staphylococcus Phages of Myoviridae. No identifiable resistance or virulence genes were found in the JD007 genome. JD007 was able to lyse 95% of S. aureus isolates, including the prevalent ST239-MRSA and ST59-MRSA strains isolated from different hospitals in Shanghai, China, and inhibition assays showed that JD007 could inhibit S. aureus growth at a multiplicity of infection of 0.1. The results suggested that Staphylococcus Phage JD007 can potentially be used in Phage therapy or for the detection of S. aureus.
Joana Azeredo - One of the best experts on this subject based on the ideXlab platform.
-
A novel flow cytometry assay based on bacterioPhage-derived proteins for Staphylococcus detection in blood
Scientific Reports, 2020Co-Authors: Susana P. Costa, Luís D. R. Melo, Joana Azeredo, Nicolina M. Dias, Sílvio B. Santos, Carla M. CarvalhoAbstract:Bloodstream infections (BSIs) are considered a major cause of death worldwide. Staphylococcus spp. are one of the most BSIs prevalent bacteria, classified as high priority due to the increasing multidrug resistant strains. Thus, a fast, specific and sensitive method for detection of these pathogens is of extreme importance. In this study, we have designed a novel assay for detection of Staphylococcus in blood culture samples, which combines the advantages of a Phage endolysin cell wall binding domain (CBD) as a specific probe with the accuracy and high-throughput of flow cytometry techniques. In order to select the biorecognition molecule, three different truncations of the C-terminus of Staphylococcus Phage endolysin E-LM12, namely the amidase (AMI), SH3 and amidase+SH3 (AMI_SH3) were cloned fused with a green fluorescent protein. From these, a higher binding efficiency to Staphylococcus cells was observed for AMI_SH3, indicating that the amidase domain possibly contributes to a more efficient binding of the SH3 domain. The novel Phage endolysin-based flow cytometry assay provided highly reliable and specific detection of 1–5 CFU of Staphylococcus in 10 mL of spiked blood, after 16 hours of enrichment culture. Overall, the method developed herein presents advantages over the standard BSIs diagnostic methods, potentially contributing to an early and effective treatment of BSIs.
-
Staphylococci Phages display vast genomic diversity and evolutionary relationships
BMC Genomics, 2019Co-Authors: Hugo Oliveira, Welkin H. Pope, Marta Sampaio, Oscar Dias, Luís D. R. Melo, Graham F. Hatfull, Joana AzeredoAbstract:BackgroundBacterioPhages are the most abundant and diverse entities in the biosphere, and this diversity is driven by constant predator–prey evolutionary dynamics and horizontal gene transfer. Phage genome sequences are under-sampled and therefore present an untapped and uncharacterized source of genetic diversity, typically characterized by highly mosaic genomes and no universal genes. To better understand the diversity and relationships among Phages infecting human pathogens, we have analysed the complete genome sequences of 205 Phages of Staphylococcus sp.ResultsThese are predicted to encode 20,579 proteins, which can be sorted into 2139 phamilies (phams) of related sequences; 745 of these are orphams and possess only a single gene. Based on shared gene content, these Phages were grouped into four clusters (A, B, C and D), 27 subclusters (A1-A2, B1-B17, C1-C6 and D1-D2) and one singleton. However, the genomes have mosaic architectures and individual genes with common ancestors are positioned in distinct genomic contexts in different clusters. The staphylococcal Cluster B siphoviridae are predicted to be temperate, and the integration cassettes are often closely-linked to genes implicated in bacterial virulence determinants. There are four unusual endolysin organization strategies found in Staphylococcus Phage genomes, with endolysins predicted to be encoded as single genes, two genes spliced, two genes adjacent and as a single gene with inter-lytic-domain secondary translational start site. Comparison of the endolysins reveals multi-domain modularity, with conservation of the SH3 cell wall binding domain.ConclusionsThis study provides a high-resolution view of staphylococcal viral genetic diversity, and insights into their gene flux patterns within and across different Phage groups (cluster and subclusters) providing insights into their evolution.