The Experts below are selected from a list of 31797 Experts worldwide ranked by ideXlab platform

Manuel Salmerón-sánchez - One of the best experts on this subject based on the ideXlab platform.

  • Engineered Full-Length Fibronectin-Hyaluronic Acid Hydrogels for Stem Cell Engineering.
    Advanced healthcare materials, 2020
    Co-Authors: Sara Trujillo, Matthew J. Dalby, Sebastián L. Vega, Kwang Hoon Song, Ana San Félix, Jason A. Burdick, Manuel Salmerón-sánchez
    Abstract:

    Mechanical cues induce a variety of downstream effects on Cells, including the regulation of Stem Cell behavior. Cell fate is typically characterized on biomaterial substrates where mechanical and chemical properties can be precisely tuned; however, most of these substrates do not recapitulate the biological complexity of the extraCellular matrix (ECM). Here, hydrogels are engineered for mechanobiological studies using two major components of the ECM: hyaluronic acid (HA) and fibronectin (FN). Rather than typical surface chemisorption of FN to substrates, the syStem contains full-length FN covalently crosslinked to HA throughout the hydrogel. The control over the mechanical properties of the hydrogel independent of the concentration of FN and the ability to culture viable Cells either on top or encapsulated within the hydrogels are shown. Interestingly, human mesenchymal Stem Cells (MSCs) experience an increase in nuclear translocation of the yes-associated protein (YAP) to the nucleus when cultured on (2D) substrates with increasing amounts of FN while maintaining constant hydrogel stiffness. However, this FN dependence on nuclear YAP translocation is not observed for MSCs encapsulated in (3D) hydrogels. This work develops complex hydrogels that recapitulate features of the ECM for the control of Stem Cells in both 2D and 3D environments.

  • Bacteria-laden microgels as autonomous three-dimensional environments for Stem Cell Engineering.
    Materials today. Bio, 2019
    Co-Authors: K. Witte, Aleixandre Rodrigo-navarro, Manuel Salmerón-sánchez
    Abstract:

    A one-step microfluidic syStem is developed in this study which enables the encapsulation of Stem Cells and genetically engineered non-pathogenic bacteria into a so-called three-dimensional (3D) pearl lace-like microgel of alginate with high level of monodispersity and Cell viability. The alginate-based microgel constitutes living materials that control Stem Cell differentiation in either an autonomous or heteronomous manner. The bacteria (Lactococcus lactis) encapsulated within the construct surface display adhesion fragments (III7-10 fragment of human fibronectin) for integrin binding while secreting growth factors (recombinant human bone morphogenetic protein-2) to induce osteogenic differentiation of human bone marrow-derived mesenchymal Stem Cells. We concentrate on interlinked pearl lace microgels that enabled us to prototype a low-cost 3D bioprinting platform with highly tunable properties.

  • Bacteria-Based Materials for Stem Cell Engineering.
    Advanced Materials, 2018
    Co-Authors: Aleixandre Rodrigo-navarro, Michaela Petaroudi, Anton V. Bryksin, Thomas H. Barker, Matthew J. Dalby, Andres J Garcia, Manuel Salmerón-sánchez
    Abstract:

    : Materials can be engineered to deliver specific biological cues that control Stem Cell growth and differentiation. However, current materials are still limited for Stem Cell Engineering as Stem Cells are regulated by a complex biological milieu that requires spatiotemporal control. Here a new approach of using materials that incorporate designed bacteria as units that can be engineered to control human mesenchymal Stem Cells (hMSCs), in a highly dynamic-temporal manner, is presented. Engineered Lactococcus lactis spontaneously colonizes a variety of material surfaces (e.g., polymers, metals, and ceramics) and is able to maintain growth and induce differentiation of hMSCs in 2D/3D surfaces and hydrogels. Controlled, dynamic, expression of fibronectin fragments supports Stem Cell growth, whereas inducible-temporal regulation of secreted bone morphogenetic protein-2 drives osteogenesis in an on-demand manner. This approach enables Stem Cell technologies using material syStems that host symbiotic interactions between eukaryotic and prokaryotic Cells.

  • Receptor control in mesenchymal Stem Cell Engineering
    Nature Reviews Materials, 2018
    Co-Authors: Matthew J. Dalby, Andres J Garcia, Manuel Salmerón-sánchez
    Abstract:

    Materials science offers a powerful tool to control mesenchymal Stem Cell (MSC) growth and differentiation into functional phenotypes. A complex interplay between the extraCellular matrix and growth factors guides MSC phenotypes in vivo . In this Review, we discuss materials-based bioEngineering approaches to direct MSC fate in vitro and in vivo , mimicking Cell–matrix–growth factor crosstalk. We first scrutinize MSC–matrix interactions and how the properties of a material can be tailored to support MSC growth and differentiation in vitro , with an emphasis on MSC self-renewal mechanisms. We then highlight important growth factor signalling pathways and investigate various materials-based strategies for growth factor presentation and delivery. Integrin–growth factor crosstalk in the context of MSC Engineering is introduced, and bioinspired material designs with the potential to control the MSC niche phenotype are considered. Finally, we summarize important milestones on the road to MSC Engineering for regenerative medicine. Material Engineering offers the possibility to guide the fate of mesenchymal Stem Cells (MSCs). This Review highlights integrin–growth factor receptor crosstalk mechanisms in MSC growth and differentiation, and material design strategies to trigger the synergistic signalling of integrins and growth factor receptors.

  • Receptor control in mesenchymal Stem Cell Engineering
    Nature Reviews Materials, 2018
    Co-Authors: Matthew J. Dalby, Andres J Garcia, Manuel Salmerón-sánchez
    Abstract:

    Material Engineering offers the possibility to guide the fate of mesenchymal Stem Cells (MSCs). This Review highlights integrin–growth factor receptor crosstalk mechanisms in MSC growth and differentiation, and material design strategies to trigger the synergistic signalling of integrins and growth factor receptors.

Daniel G. Anderson - One of the best experts on this subject based on the ideXlab platform.

  • Combinatorial and rational approaches to polymer synthesis for medicine.
    Advanced drug delivery reviews, 2008
    Co-Authors: Michael Goldberg, Kerry Peter Mahon, Daniel G. Anderson
    Abstract:

    High-throughput, combinatorial methods have revolutionized small molecule synthesis and drug discovery. By combining automation, miniaturization, and parallel synthesis techniques, large collections of new compounds have been synthesized and screened. It is becoming increasingly clear that these same approaches can also assist the discovery and development of novel biomaterials for medicine. This review examines combinatorial and rational polymer synthesis for medical applications, including Stem Cell Engineering and nucleic acid drug delivery.

  • The development of high-throughput screening approaches for Stem Cell Engineering.
    Current opinion in chemical biology, 2007
    Co-Authors: Ying Mei, Michael Goldberg, Daniel G. Anderson
    Abstract:

    It has become increasingly clear that both soluble factors, such as growth factors, and insoluble factors, including the surfaces on which Cells grow, can have controlling effects on Stem Cell behavior and differentiation. While much progress has been made in biomaterial design and application, the rational design of biomaterial cues to direct Stem Cell behavior and differentiation remains challenging. Recent advances in automated, high-throughput methods for synthesizing and screening combinatorial biomaterial libraries and Cellular microenvironments promise to accelerate the discovery of factors that control Stem Cell behavior. Specific examples include miniaturized, automated, combinatorial material synthesis and extraCellular matrix screening methods as well microarrayed methods for creating local microenvironments of soluble factors, such as small molecules, siRNA, and other signaling molecules.

Melissa A Kinney - One of the best experts on this subject based on the ideXlab platform.

  • Author Correction: A syStems biology pipeline identifies regulatory networks for Stem Cell Engineering.
    Nature biotechnology, 2019
    Co-Authors: Melissa A Kinney, Jenna M Frame, Jessica Barragan, Ashlee J. Conway, Kwok-kin Wong, Patrick Cahan, James J Collins, Trista E. North
    Abstract:

    In the version of this article initially published, the second NIH grant "R24-DK49216" to author George Q. Daley contained an error. The grant number should have read U54DK110805. The error has been corrected in the HTML and PDF versions of the article.

  • a syStems biology pipeline identifies regulatory networks for Stem Cell Engineering
    Nature Biotechnology, 2019
    Co-Authors: Melissa A Kinney, Linda T Vo, Jenna M Frame, Jessica Barragan
    Abstract:

    A major challenge for Stem Cell Engineering is achieving a holistic understanding of the molecular networks and biological processes governing Cell differentiation. To address this challenge, we describe a computational approach that combines gene expression analysis, previous knowledge from proteomic pathway informatics and Cell signaling models to delineate key transitional states of differentiating Cells at high resolution. Our network models connect sparse gene signatures with corresponding, yet disparate, biological processes to uncover molecular mechanisms governing Cell fate transitions. This approach builds on our earlier CellNet and recent trajectory-defining algorithms, as illustrated by our analysis of hematopoietic specification along the erythroid lineage, which reveals a role for the EGF receptor family member, ErbB4, as an important mediator of blood development. We experimentally validate this prediction and perturb the pathway to improve erythroid maturation from human pluripotent Stem Cells. These results exploit an integrative syStems perspective to identify new regulatory processes and nodes useful in Cell Engineering. An advanced CellNet pipeline models the dynamics of Stem Cell differentiation.

  • A syStems biology pipeline identifies regulatory networks for Stem Cell Engineering.
    Nature Biotechnology, 2019
    Co-Authors: Melissa A Kinney, Linda T Vo, Jenna M Frame, Jessica Barragan, Ashlee J. Conway, Kwok-kin Wong, Patrick Cahan, James J Collins, Shuai Li, Trista E. North
    Abstract:

    A major challenge for Stem Cell Engineering is achieving a holistic understanding of the molecular networks and biological processes governing Cell differentiation. To address this challenge, we describe a computational approach that combines gene expression analysis, previous knowledge from proteomic pathway informatics and Cell signaling models to delineate key transitional states of differentiating Cells at high resolution. Our network models connect sparse gene signatures with corresponding, yet disparate, biological processes to uncover molecular mechanisms governing Cell fate transitions. This approach builds on our earlier CellNet and recent trajectory-defining algorithms, as illustrated by our analysis of hematopoietic specification along the erythroid lineage, which reveals a role for the EGF receptor family member, ErbB4, as an important mediator of blood development. We experimentally validate this prediction and perturb the pathway to improve erythroid maturation from human pluripotent Stem Cells. These results exploit an integrative syStems perspective to identify new regulatory processes and nodes useful in Cell Engineering.

Jessica Barragan - One of the best experts on this subject based on the ideXlab platform.

  • Author Correction: A syStems biology pipeline identifies regulatory networks for Stem Cell Engineering.
    Nature biotechnology, 2019
    Co-Authors: Melissa A Kinney, Jenna M Frame, Jessica Barragan, Ashlee J. Conway, Kwok-kin Wong, Patrick Cahan, James J Collins, Trista E. North
    Abstract:

    In the version of this article initially published, the second NIH grant "R24-DK49216" to author George Q. Daley contained an error. The grant number should have read U54DK110805. The error has been corrected in the HTML and PDF versions of the article.

  • a syStems biology pipeline identifies regulatory networks for Stem Cell Engineering
    Nature Biotechnology, 2019
    Co-Authors: Melissa A Kinney, Linda T Vo, Jenna M Frame, Jessica Barragan
    Abstract:

    A major challenge for Stem Cell Engineering is achieving a holistic understanding of the molecular networks and biological processes governing Cell differentiation. To address this challenge, we describe a computational approach that combines gene expression analysis, previous knowledge from proteomic pathway informatics and Cell signaling models to delineate key transitional states of differentiating Cells at high resolution. Our network models connect sparse gene signatures with corresponding, yet disparate, biological processes to uncover molecular mechanisms governing Cell fate transitions. This approach builds on our earlier CellNet and recent trajectory-defining algorithms, as illustrated by our analysis of hematopoietic specification along the erythroid lineage, which reveals a role for the EGF receptor family member, ErbB4, as an important mediator of blood development. We experimentally validate this prediction and perturb the pathway to improve erythroid maturation from human pluripotent Stem Cells. These results exploit an integrative syStems perspective to identify new regulatory processes and nodes useful in Cell Engineering. An advanced CellNet pipeline models the dynamics of Stem Cell differentiation.

  • A syStems biology pipeline identifies regulatory networks for Stem Cell Engineering.
    Nature Biotechnology, 2019
    Co-Authors: Melissa A Kinney, Linda T Vo, Jenna M Frame, Jessica Barragan, Ashlee J. Conway, Kwok-kin Wong, Patrick Cahan, James J Collins, Shuai Li, Trista E. North
    Abstract:

    A major challenge for Stem Cell Engineering is achieving a holistic understanding of the molecular networks and biological processes governing Cell differentiation. To address this challenge, we describe a computational approach that combines gene expression analysis, previous knowledge from proteomic pathway informatics and Cell signaling models to delineate key transitional states of differentiating Cells at high resolution. Our network models connect sparse gene signatures with corresponding, yet disparate, biological processes to uncover molecular mechanisms governing Cell fate transitions. This approach builds on our earlier CellNet and recent trajectory-defining algorithms, as illustrated by our analysis of hematopoietic specification along the erythroid lineage, which reveals a role for the EGF receptor family member, ErbB4, as an important mediator of blood development. We experimentally validate this prediction and perturb the pathway to improve erythroid maturation from human pluripotent Stem Cells. These results exploit an integrative syStems perspective to identify new regulatory processes and nodes useful in Cell Engineering.

David V. Schaffer - One of the best experts on this subject based on the ideXlab platform.

  • A global assessment of Stem Cell Engineering.
    Tissue Engineering Part A, 2014
    Co-Authors: Jeanne F. Loring, Todd C. Mcdevitt, David V. Schaffer, Peter W. Zandstra, Sean P. Palecek, Robert M. Nerem
    Abstract:

    Over the last 2 years a global assessment of Stem Cell Engineering (SCE) was conducted with the sponsorship of the National Science Foundation, the National Cancer Institute at the National Institutes of Health, and the National Institute of Standards and Technology. The purpose was to gather information on the worldwide status and trends in SCE, that is, the involvement of engineers and Engineering approaches in the Stem Cell field, both in basic research and in the translation of research into clinical applications and commercial products. The study was facilitated and managed by the World Technology Evaluation Center. The process involved site visits in both Asia and Europe, and it also included several different workshops. From this assessment, the panel concluded that there needs to be an increased role for engineers and the Engineering approach. This will provide a foundation for the generation of new markets and future economic growth. To do this will require an increased investment in Engineering,...

  • Progress and Prospects for Stem Cell Engineering
    Annual review of chemical and biomolecular engineering, 2011
    Co-Authors: Randolph S. Ashton, Albert J. Keung, Joseph Peltier, David V. Schaffer
    Abstract:

    Stem Cells offer tremendous biomedical potential owing to their abilities to self-renew and differentiate into Cell types of multiple adult tissues. Researchers and engineers have increasingly developed novel discovery technologies, theoretical approaches, and Cell culture syStems to investigate microenvironmental cues and Cellular signaling events that control Stem Cell fate. Many of these technologies facilitate high-throughput investigation of microenvironmental signals and the intraCellular signaling networks and machinery processing those signals into Cell fate decisions. As our aggregate empirical knowledge of Stem Cell regulation grows, theoretical modeling with syStems and computational biology methods has and will continue to be important for developing our ability to analyze and extract important conceptual features of Stem Cell regulation from complex data. Based on this body of knowledge, Stem Cell engineers will continue to develop technologies that predictably control Stem Cell fate with the ultimate goal of being able to accurately and economically scale up these syStems for clinical-grade production of Stem Cell therapeutics.

  • Neural Stem Cell Engineering: directed differentiation of adult and embryonic Stem Cells into neurons.
    Frontiers in bioscience : a journal and virtual library, 2008
    Co-Authors: Matthew J. Robertson, Phung Gip, David V. Schaffer
    Abstract:

    Both adult neural Stem Cells and embryonic Stem Cells have shown the capacity to differentiation into multiple Cell types of the adult nervous syStem. They will therefore serve as valuable syStems for basic investigations of Cell fate choice mechanisms, as well as play important future roles in applications ranging from regenerative medicine to drug screening. However, there are significant challenges remaining, including the identification of signaling factors that specify Cell fate in the Stem Cell niche, the analysis of intraCellular targets and mechanisms of these extraCellular signals, and the development of ex vivo culture syStems that can exert efficient control over Cell function. This review will discuss progress in the identification of signaling mechanisms and culture syStems that regulate neural differentiation, neuronal differentiation, and neuronal subtype specification.