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Antonino Pollio - One of the best experts on this subject based on the ideXlab platform.

  • Alkaline direct transesterification of different species of Stichococcus for bio-oil production
    New biotechnology, 2016
    Co-Authors: Immacolata Gargano, Gabriele Pinto, Raffaele Marotta, Roberto Andreozzi, Giuseppe Olivieri, Antonio Marzocchella, Danilo Spasiano, Antonino Pollio
    Abstract:

    The cost of bio-oil refining from microalgal biomass can be significantly reduced by combining extraction and transesterification. The characterisation and optimisation of the combined steps have been carried out on strains of Stichococcus bacillaris, focusing on catalyst type and concentration, reaction time and temperature, methanol/biomass ratio, pre-mixing time and water content in the biomass. The bio-oil yield has been referenced as production of fatty acid methyl esters (FAMEs). The maximum yield (∼17%) was achieved using dried biomass with alkaline catalyst at 60 °C and methanol/biomass weight ratio of 79:1. Alkaline catalyst conditions gave faster reaction rates and higher bio-oil yields than acid catalyst. Yield was also strongly affected by water content in the biomass. A mechanistic interpretation has been proposed to elucidate the effect of the different operating conditions. However, the structural characteristics of the Chlorophyta cell wall can be very different, leading to different bio-oil yields when the same protocol is applied. Therefore, the optimised protocol of direct transesterification for Stichococcus bacillaris strains was tested on other Stichococcus strains and several other Chlorophyta species characterised by a different cell wall structure. It was clearly demonstrated that different results for bio-oil yield were obtained within the same microalgal species and much more within different microalgal genera.

  • Biodiesel production from Stichococcus bacillaris in lab-scale photobioreactors
    2012
    Co-Authors: G. Olivieri, Antonio Marzocchella, I. Gargano, R. Andreozzi, R. Marotta, G. Pinto, Antonino Pollio
    Abstract:

    Key aspects of the industrialization of microalgae-to-biofuel processes are: i) selection and improvements of algal strains; ii) development of high-performance photobioreactors; iii) selection of culture optimal conditions; iv) improvement of oil extraction and transesterification processes; v) development of microalgal-based biorefineries. An accurate design of nutrient selection and irradiance strategy appears to improve remarkably yield and productivity of both biomass and total lipid content). Present contribution regards the effects of both the CO2 oncentration in the gas phase and the pH of the culture medium on biodiesel production by Stichococcus bacillaris (ACUF 158/11). Two typologies of photobioreactors were investigated: 0.6 L vertical bubble column (VBC) and 1.7 L inclined bubble column (IBC). Indoor cultures of S. bacillaris were carried out under batch, fed-batch and semi-continuous conditions with respect to the liquid phase. Tests were carried out at 23°C and at continuous artificial light, irradiance 300 micronE/(m2 s). The CO2 concentration in the gas phase fed at the photobioreactors (0.5 vvm) was increased up to 15% v to simulate the CO2 concentration level in exhaust gas. The pH of the liquid culture ranged between 3.0 and 8.5. Results showed that CO2 concentration increase stimulates the microalgal growth improving the process performances when expressed in terms of biomass and lipid productivity. Tests carried out sparging gas at 5%V CO2 and at different values of pH showed that the biomass productivity is optimized at pH close to 7.0 for the investigated strain. The hydrodynamics of the two photobioreactor configurations investigated (IBC and VBC) has significant effects on the process productivity. Tests carried out in IBC photobioreactors were characterized by biomass productivity of 0.256 g/(L day) and lipid productivity 0.080 g/(L day), larger than that assessed in VBC photobioreactors, 0.124 and 0.042 g/(L day), respectively

  • Biodiesel production from Stichococcus strains at laboratory scale
    Journal of Chemical Technology & Biotechnology, 2011
    Co-Authors: Giuseppe Olivieri, Gabriele Pinto, Roberto Andreozzi, Antonio Marzocchella, Antonino Pollio
    Abstract:

    BACKGROUND: This paper reports the results of an experimental campaign of autotrophic cultures of Stichococcus strains aiming at selecting the most promising strain for biofuel production. The strain selected—S. bacillaris 158/11—was cultivated in 1 L lab-scale bubble column photobioreactors under fed-batch and semi-continuous conditions. A Bold basal medium supplemented with NaNO3 as nitrogen source was adopted. Tests were carried out at 23 °C, 140 µE m−2 s−1, and air flow rate ranging between 0.4 and 4 vvm. Cultures were characterized in terms of pH, concentration of total nitrogen, total organic carbon, total inorganic carbon, biomass, lipid fraction and methyl-ester distribution of transesterified lipids. RESULTS:S. bacillaris 158/11 proved to be the best strain to produce biodiesel. Methyl-ester distribution was characterized by a large fraction of methyl palmitate, methyl linolenate, methyl linoleate, and methyl oleate along with phytol. The process photosynthetic efficiency—fraction of available light stored as chemical energy - was about 1.5%. Specific biomass productivity was ∼60 mgDM L−1 day−1 under the semi-continuous conditions tested. Total lipid productivity was 14 mg L−1 day−1 at a dilution rate of 0.050 L day−1. CONCLUSION:S. bacillaris 158/11 is a potential strain for massive microalgae cultures for biofuel production. Higher biomass/total-lipid productivity could be obtained in sunlight. Copyright © 2011 Society of Chemical Industry

  • Biodiesel production from Stichococcus strains on laboratory scale
    'Wiley', 2011
    Co-Authors: Giuseppe Olivieri, Gabriele Pinto, Roberto Andreozzi, Antonio Marzocchella, Antonino Pollio
    Abstract:

    The paper reports the results of an experimental campaign of autotrophic cultures of Stichococcus strains aiming at selecting the most promising strain for biofuel production. The strain selected - S. bacillaris 158/11 - was cultivated in 1 L lab-scale bubble column photobioreactors under fed-batch and semi-continuous conditions. A Bold basal medium supplemented with NaNO3 as nitrogen source was adopted. Tests were carried out at 23 °C, 140 microE/(m2s), and air flow rate ranging between 0.4 and 4 vvm. Cultures were characterized in terms of pH, concentration of total nitrogen, total organic carbon, total inorganic carbon, biomass, lipid fraction and methyl-ester distribution of transesterified lipids. S. bacillaris 158/11 proved to be the best strain to produce biodiesel. Methyl-ester distribution was characterized by a large fraction of methyl palmitate, methyl linolenate, methyl linoleate, and methyl oleate along with phytol. The process photosynthetic efficiency – fraction of available light stored as chemical energy - was about 1.5%. Specific biomass productivity was ~60 mgDM/(L•day) under the semi-continuous conditions tested. Total lipid productivity was 14 mg/(L•day) at a dilution rate of 0.050 1/day. S. bacillaris 158/11 is a potential strain for massive microalgae cultures for biofuel production. Higher biomass/total-lipid productivity could be obtained at sunlight

  • Carbon dioxide sequestration and bio-oil production by Stichococcus strains
    2010
    Co-Authors: Giuseppe Olivieri, Gabriele Pinto, Antonino Pollio, Roberto Andreozzi, Antonio Marzocchella, Piero Salatino
    Abstract:

    The present study reports results of an experimental campaign of autotrophic cultures of Stichococcus strains in lab-scale bubble columns operated as photobioreactors. Air was sparged at the bottom through a porous ceramic distributor. Microalgae growth and lipid production were investigated at 23°C and 140 E/ (m 2 s) light irradiance. Lipids were extracted from dried microalgae and transesterified with alkaline methanol. The lipid content/distribution of the bio-oil was assessed. The effects of the air volumetric flow rate and operation modality with respect to the liquid phase – batch, fed-batch and semicontinuous were investigated.

Jiří Neustupa - One of the best experts on this subject based on the ideXlab platform.

  • Pseudomarvania, gen. nov. (Chlorophyta, Trebouxiophyceae), a new genus for “budding ” subaerial green algae Marvania aerophytica Neustupa et ŠejNohová and Stichococcus ampulliformis haNda
    2016
    Co-Authors: Marek Eliáš, Jiří Neustupa
    Abstract:

    Abstract: Several unicellular green algae exhibit a unique type of cell division, which can be likened to budding. These algae comprise Spongiochrysis hawaiiensis in Cladophorales (Ulvophyceae) and Stichococcus ampulliformis and species of the genus Marvania in the class Trebouxiophyceae. We determined the 18S rRNA gene sequence from Marvania aerophytica NEustupa et šEjNohová and inferred its phylogenetic position. Our analyses demonstrated that M. aerophytica is unrelated to other species of the genus Marvania (including the type species M. geminata), but together with S. ampulliformis forms a lineage within a broader clade comprising also species of the order Prasiolales, Desmococcus spp., Gloeotilla spp., and non-monophyletic Stichococcus spp. We discuss morphological characteristics of M. aerophytica and S. ampulliformis and based on our results, we propose M. aerophytica and S. ampulliformis be reclassified into a newly established genus of “budding ” green algae, Pseudomarvania, as Pseudomarvania aerophytica, comb. nov. and Pseudomarvania ampulliformis, comb. nov

  • Pseudomarvania, gen. nov. (Chlorophyta, Trebouxiophyceae), a new genus for "budding" subaerial green algae Marvania aerophytica NEUSTUPA et ŠEJNOHOVÁ and Stichococcus ampulliformis HANDA.
    Fottea, 2009
    Co-Authors: Marek Eliáš, Jiří Neustupa
    Abstract:

    Several unicellular green algae exhibit a unique type of cell division, which can be likened to budding. These algae comprise Spongiochrysis hawaiiensis in Cladophorales (Ulvophyceae) and Stichococcus ampulliformis and species of the genus Marvania in the class Trebouxiophyceae. We determined the 18S rRNA gene sequence from Marvania aerophytica NEUSTUPA et SEJNOHOVA and inferred its phylogenetic position. Our analyses demonstrated that M. aerophytica is unrelated to other species of the genus Marvania (including the type species M. geminata), but together with S. ampulliformis forms a lineage within a broader clade comprising also species of the order Prasiolales, Desmococcus spp., Gloeotilla spp., and non-monophyletic Stichococcus spp. We discuss morphological characteristics of M. aerophytica and S. ampulliformis and based on our results, we propose M. aerophytica and S. ampulliformis be reclassified into a newly established genus of "budding" green algae, Pseudomarvania, as Pseudomarvania aerophytica, comb. nov. and Pseudomarvania ampulliformis, comb. nov.

Jackson O. Lay - One of the best experts on this subject based on the ideXlab platform.

  • Bioprocessing of Stichococcus bacillaris strain siva2011
    Biotechnology for biofuels, 2014
    Co-Authors: Ganapathy Sivakumar, Kwangkook Jeong, Jackson O. Lay
    Abstract:

    Globally, the development of a cost-effective long-term renewable energy infrastructure is one of the most challenging problems faced by society today. Microalgae are rich in potential biofuel substrates such as lipids, including triacylglycerols (TAGs). Some of these algae also biosynthesize small molecule hydrocarbons. These hydrocarbons can often be used as liquid fuels, often with more versatility and by a more direct approach than some TAGs. However, the appropriate TAGs, accumulated from microalgae biomass, can be used as substrates for different kinds of renewable liquid fuels such as biodiesel and jet fuel. This article describes the isolation and identification of a lipid-rich, hydrocarbon-producing alga, Stichococcus bacillaris strain siva2011, together with its bioprocessing, hydrocarbon and fatty acid methyl ester (FAME) profiles. The S. bacillaris strain siva2011 was scaled-up in an 8 L bioreactor with 0.2% CO2. The C16:0, C16:3, C18:1, C18:2 and C18:3 were 112.2, 9.4, 51.3, 74.1 and 69.2 mg/g dry weight (DW), respectively. This new strain produced a significant amount of biomass of 3.79 g/L DW on day 6 in the 8 L bioreactor and also produced three hydrocarbons. A new oil-rich microalga S. bacillaris strain siva2011 was discovered and its biomass has been scaled-up in a newly designed balloon-type bioreactor. The TAGs and hydrocarbons produced by this organism could be used as substrates for jet fuel or biodiesel.

  • Bioprocessing of Stichococcus bacillaris strain
    2014
    Co-Authors: Ganapathy Sivakumar, Kwangkook Jeong, Jackson O. Lay
    Abstract:

    Background: Globally, the development of a cost-effective long-term renewable energy infrastructure is one of the most challenging problems faced by society today. Microalgae are rich in potential biofuel substrates such as lipids, including triacylglycerols (TAGs). Some of these algae also biosynthesize small molecule hydrocarbons. These hydrocarbons can often be used as liquid fuels, often with more versatility and by a more direct approach than some TAGs. However, the appropriate TAGs, accumulated from microalgae biomass, can be used as substrates for different kinds of renewable liquid fuels such as biodiesel and jet fuel. Results: This article describes the isolation and identification of a lipid-rich, hydrocarbon-producing alga, Stichococcus bacillaris strain siva2011, together with its bioprocessing, hydrocarbon and fatty acid methyl ester (FAME) profiles. The S. bacillaris strain siva2011 was scaled-up in an 8 L bioreactor with 0.2% CO2. The C16:0, C16:3, C18:1, C18:2 and C18:3 were 112.2, 9.4, 51.3, 74.1 and 69.2 mg/g dry weight (DW), respectively. This new strain produced a significant amount of biomass of 3.79 g/L DW on day 6 in the 8 L bioreactor and also produced three hydrocarbons. Conclusions: A new oil-rich microalga S. bacillaris strain siva2011 was discovered and its biomass has been scaled-up in a newly designed balloon-type bioreactor. The TAGs and hydrocarbons produced by this organism could be used as substrates for jet fuel or biodiesel.

Marek Eliáš - One of the best experts on this subject based on the ideXlab platform.

  • Pseudomarvania, gen. nov. (Chlorophyta, Trebouxiophyceae), a new genus for “budding ” subaerial green algae Marvania aerophytica Neustupa et ŠejNohová and Stichococcus ampulliformis haNda
    2016
    Co-Authors: Marek Eliáš, Jiří Neustupa
    Abstract:

    Abstract: Several unicellular green algae exhibit a unique type of cell division, which can be likened to budding. These algae comprise Spongiochrysis hawaiiensis in Cladophorales (Ulvophyceae) and Stichococcus ampulliformis and species of the genus Marvania in the class Trebouxiophyceae. We determined the 18S rRNA gene sequence from Marvania aerophytica NEustupa et šEjNohová and inferred its phylogenetic position. Our analyses demonstrated that M. aerophytica is unrelated to other species of the genus Marvania (including the type species M. geminata), but together with S. ampulliformis forms a lineage within a broader clade comprising also species of the order Prasiolales, Desmococcus spp., Gloeotilla spp., and non-monophyletic Stichococcus spp. We discuss morphological characteristics of M. aerophytica and S. ampulliformis and based on our results, we propose M. aerophytica and S. ampulliformis be reclassified into a newly established genus of “budding ” green algae, Pseudomarvania, as Pseudomarvania aerophytica, comb. nov. and Pseudomarvania ampulliformis, comb. nov

  • Pseudomarvania, gen. nov. (Chlorophyta, Trebouxiophyceae), a new genus for "budding" subaerial green algae Marvania aerophytica NEUSTUPA et ŠEJNOHOVÁ and Stichococcus ampulliformis HANDA.
    Fottea, 2009
    Co-Authors: Marek Eliáš, Jiří Neustupa
    Abstract:

    Several unicellular green algae exhibit a unique type of cell division, which can be likened to budding. These algae comprise Spongiochrysis hawaiiensis in Cladophorales (Ulvophyceae) and Stichococcus ampulliformis and species of the genus Marvania in the class Trebouxiophyceae. We determined the 18S rRNA gene sequence from Marvania aerophytica NEUSTUPA et SEJNOHOVA and inferred its phylogenetic position. Our analyses demonstrated that M. aerophytica is unrelated to other species of the genus Marvania (including the type species M. geminata), but together with S. ampulliformis forms a lineage within a broader clade comprising also species of the order Prasiolales, Desmococcus spp., Gloeotilla spp., and non-monophyletic Stichococcus spp. We discuss morphological characteristics of M. aerophytica and S. ampulliformis and based on our results, we propose M. aerophytica and S. ampulliformis be reclassified into a newly established genus of "budding" green algae, Pseudomarvania, as Pseudomarvania aerophytica, comb. nov. and Pseudomarvania ampulliformis, comb. nov.

Fabio Temussi - One of the best experts on this subject based on the ideXlab platform.