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Bingru Huang - One of the best experts on this subject based on the ideXlab platform.
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comparative transcriptomic analysis reveals common molecular factors responsive to heat and drought stress in agrostis Stolonifera
Scientific Reports, 2018Co-Authors: Bingru HuangAbstract:Heat and drought stress are primary abiotic stresses confining growth of cool-season grass species during summer. The objective of this study was to identify common molecular factors and metabolic pathways associated with heat and drought responses in creeping bentgrass (Agrostis Stolonifera) by comparative analysis of transcriptomic profiles between plants exposed to heat and drought stress. Plants were exposed to heat stress (35/30 °C day/night temperature) or drought stress by withholding irrigation for 21 d in growth chambers. Transcriptomic profiling by RNA-seq in A. Stolonifera (cv. ‘Penncross’) found 670 commonly up-regulated and 812 commonly down-regulated genes by heat and drought stress. Transcriptional up-regulations of differentially expressed genes (DEGs) due to heat and drought stress include genes that were highly enriched in oxylipin biosynthetic process and proline biosynthetic process. Transcriptional down-regulations of genes under heat and drought stress were highly enriched and involved in thiamine metabolic process and calcium sensing receptor. These commonly-regulated genes by heat and drought stress identified in A. Stolonifera suggested that drought and heat responses shared such common molecular factors and pathways, which could be potential candidate genes for genetic modification of improving plant tolerance to the combined heat and drought stress.
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transcriptomic analysis reveals unique molecular factors for lipid hydrolysis secondary cell walls and oxidative protection associated with thermotolerance in perennial grass
BMC Genomics, 2018Co-Authors: Bingru HuangAbstract:Heat stress is the primary abiotic stress limiting growth of cool-season grass species. The objective of this study was to determine molecular factors and metabolic pathways associated with superior heat tolerance in thermal bentgrass (Agrostis scabra) by comparative analysis of transcriptomic profiles with its co-generic heat-sensitive species creeping bentgrass (A. Stolonifera). Transcriptomic profiling by RNA-seq in both heat-sensitive A. Stolonifera (cv. ‘Penncross’) and heat-tolerant A. scabra exposed to heat stress found 1393 (675 up- and 718 down-regulated) and 1508 (777 up- and 731 down-regulated) differentially-expressed genes, respectively. The superior heat tolerance in A. scabra was associated with more up-regulation of genes in oxidative protection, proline biosynthesis, lipid hydrolysis, hemicellulose and lignin biosynthesis, compared to heat-sensitive A. Stolonifera. Several transcriptional factors (TFs), such as high mobility group B protein 7 (HMGB7), dehydration-responsive element-binding factor 1a (DREB1a), multiprotein-bridging factor 1c (MBF1c), CCCH-domain containing protein 47 (CCCH47), were also found to be up-regulated in A. scabra under heat stress. The unique TFs and genes identified in thermal A. scabra could be potential candidate genes for genetic modification of cultivated grass species for improving heat tolerance, and the associated pathways could contribute to the transcriptional regulation for superior heat tolerance in bentgrass species.
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Transcriptomic analysis reveals unique molecular factors for lipid hydrolysis, secondary cell-walls and oxidative protection associated with thermotolerance in perennial grass
BMC Genomics, 2018Co-Authors: Bingru HuangAbstract:Background Heat stress is the primary abiotic stress limiting growth of cool-season grass species. The objective of this study was to determine molecular factors and metabolic pathways associated with superior heat tolerance in thermal bentgrass ( Agrostis scabra ) by comparative analysis of transcriptomic profiles with its co-generic heat-sensitive species creeping bentgrass ( A. Stolonifera ). Results Transcriptomic profiling by RNA-seq in both heat-sensitive A. Stolonifera (cv. ‘Penncross’) and heat-tolerant A. scabra exposed to heat stress found 1393 (675 up- and 718 down-regulated) and 1508 (777 up- and 731 down-regulated) differentially-expressed genes, respectively. The superior heat tolerance in A. scabra was associated with more up-regulation of genes in oxidative protection, proline biosynthesis, lipid hydrolysis, hemicellulose and lignin biosynthesis, compared to heat-sensitive A. Stolonifera . Several transcriptional factors (TFs), such as high mobility group B protein 7 (HMGB7), dehydration-responsive element-binding factor 1a (DREB1a), multiprotein-bridging factor 1c (MBF1c), CCCH-domain containing protein 47 (CCCH47), were also found to be up-regulated in A. scabra under heat stress. Conclusions The unique TFs and genes identified in thermal A. scabra could be potential candidate genes for genetic modification of cultivated grass species for improving heat tolerance, and the associated pathways could contribute to the transcriptional regulation for superior heat tolerance in bentgrass species.
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Additional file 1: of Transcriptomic analysis reveals unique molecular factors for lipid hydrolysis, secondary cell-walls and oxidative protection associated with thermotolerance in perennial grass
2018Co-Authors: Bingru HuangAbstract:Heat map of GO term enrichment analysis for up-regulated DEGs in A. Stolonifera (P) and A. scabra (N). Scale represents log10 of P-value in the enrichment analysis. (JPEG 575 kb
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differential proteomic response to heat stress in thermal agrostis scabra and heat sensitive agrostis Stolonifera
Physiologia Plantarum, 2010Co-Authors: Bingru HuangAbstract:Knowledge of heat-responsive proteins is critical for further understanding of the molecular mechanisms of heat tolerance. The objective of this study was to compare proteins differentially expressed in two C(3) grass species contrasting in heat tolerance, heat-tolerant thermal Agrostis scabra and heat-sensitive Agrostis Stolonifera L., and to identify heat-responsive proteins for short- and long-term responses. Plants were exposed to 20/15 degrees C (day/night, control) or 40/35 degrees C (day/night, heat stress) in growth chambers. Leaves were harvested at 2 and 10 days after temperature treatment. Proteins were extracted and separated by fluorescence difference gel electrophoresis (DIGE). Thermal A. scabra had superior heat tolerance than A. Stolonifera, as indicated by the maintenance of higher chlorophyll content and photochemical efficiency under heat stress. The two-dimensional difference electrophoresis detected 68 heat-responsive proteins in the two species. Thermal A. scabra had more protein spots either down- or up-regulated at 2 days of heat stress, but fewer protein spots were altered at 10 days of heat stress compared with A. Stolonifera. Many protein spots exhibited transient down-regulation in thermal A. scabra (only at 2 days of heat treatment), whereas down-regulation of many proteins was also found at 10 days of heat treatment in A. Stolonifera, which suggested that protein metabolism in thermal A. scabra might acclimate to heat stress more rapidly than those in A. Stolonifera. The sequences of 56 differentially expressed protein spots were identified using mass spectrometry. The results suggest that the maintenance or less severe down-regulation of proteins during long-term (10 days) heat stress may contribute to the superior heat tolerance in thermal A. scabra, including those involved in photosynthesis [RuBisCo, RuBisCo activase, chloroplastic glyceraldehydes-3-phosphate dehydrogenase (GAPDH), chloroplastic aldolase, oxygen-evolving complex, photosystem I subunits], dark respiration (cytosolic GAPDH, cytoplasmic aldolase, malate dehydrogenase, hydroxypyruvate reductase, sedoheptulose-1,7-bisphosphatase), photorespiration [(hydroxypyruvate reductase, alanine aminotransferase (AlaAT), hydroxymethyltransferase (SHMT), glycine decarboxylase (GDC)], as well as heat and oxidative stress protection [heat shock cognate (HSC) 70 and FtsH-like protein].
Jae Sue Choi - One of the best experts on this subject based on the ideXlab platform.
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Anti-adipogenic activity of the edible brown alga Ecklonia Stolonifera and its constituent fucosterol in 3T3-L1 adipocytes
Archives of Pharmacal Research, 2014Co-Authors: Hyun Ah Jung, Hee Jin Jung, Hyun Young Jeong, Hyun Ju Kwon, Min-sun Kim, Jae Sue ChoiAbstract:Fucosterol is a sterol metabolite of brown algae and regulates genes involved with cholesterol homeostasis. As a part of our continuous search for anti-obesity agents from natural marine sources, the anti-adipogenic activities of Ecklonia Stolonifera and its sterol, fucosterol, were evaluated for the inhibition of adipocyte differentiation and lipid formation. Oil Red O staining was used to evaluate triglyceride contents in 3T3-L1 pre-adipocytes primed by differentiation medium (DM) I and DM II. The methanolic extract of E. Stolonifera showed strong anti-adipogenic activity, and was thus fractionated with several solvents. Among the tested fractions, the dichloromethane (CH_2Cl_2) fraction was found to be the most active fraction, with significant inhibition (40.5 %) of intracellular lipid accumulation at a non-toxic concentration, followed by the ethyl acetate fraction (30.2 %) at the same concentration, while the n -butanol and water fractions did not show inhibitory activity within the tested concentrations. The strong anti-adipogenic CH_2Cl_2-soluble fraction was further purified by a repeated chromatography to yield fucosterol. Fucosterol reduced lipid contents in a concentration-dependent manner without showing any cytotoxicity. Fucosterol treatment also yielded a decrease in the expression of the adipocyte marker proteins peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα) in a concentration-dependent manner. Taken together, these results suggest that fucosterol inhibits expression of PPARγ and C/EBPα, resulting in a decrease of lipid accumulation in 3T3-L1 pre-adipocytes, indicating that the potential use of E. Stolonifera and its bioactive fucosterol as an anti-obesity agent.
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Protective effect of the edible brown alga Ecklonia Stolonifera on doxorubicin-induced hepatotoxicity in primary rat hepatocytes.
The Journal of pharmacy and pharmacology, 2014Co-Authors: Hyun Ah Jung, Jae-i Kim, Se-young Choung, Jae Sue ChoiAbstract:Objectives As part of our efforts to isolate anti-hepatotoxic agents from marine natural products, we screened the ability of 14 edible varieties of Korean seaweed to protect against doxorubicin-induced hepatotoxicity in primary rat hepatocytes. Methods Among the crude extracts of two Chlorophyta (Codium fragile and Capsosiphon fulvescens), seven Phaeophyta (Undaria pinnatifida, Sargassum thunbergii, Pelvetia siliquosa, Ishige okamurae, Ecklonia cava, Ecklonia Stolonifera and Eisenia bicyclis), five Rhodophyta (Chondrus ocellatus, Gelidium amansii, Gracilaria verrucosa, Symphycladia latiuscula and Porphyra tenera), and the extracts of Ecklonia Stolonifera, Ecklonia cava, Eisenia bicyclis and Pelvetia siliquosa exhibited significant protective effects on doxorubicin-induced hepatotoxicity, with half maximal effective concentration (EC50) values of 2.0, 2.5, 3.0 and 15.0 μg/ml, respectively. Key findings Since Ecklonia Stolonifera exhibits a significant protective potential and is frequently used as foodstuff, we isolated six phlorotannins, including phloroglucinol (1), dioxinodehydroeckol (2), eckol (3), phlorofucofuroeckol A (4), dieckol (5) and triphloroethol-A (6). Phlorotannins 2 ∼ 6 exhibited potential protective effects on doxorubicin-induced hepatotoxicity, with corresponding EC50 values of 3.4, 8.3, 4.4, 5.5 and 11.5 μg/ml, respectively. Conclusion The results clearly demonstrated that the anti-hepatotoxic effects of Ecklonia Stolonifera and its isolated phlorotannins are useful for further exploration and development of therapeutic modalities for treatment of hepatotoxicity.
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anti inflammatory activities of an ethanol extract of ecklonia Stolonifera in lipopolysaccharide stimulated raw 264 7 murine macrophage cells
Journal of Agricultural and Food Chemistry, 2012Co-Authors: Minsup Lee, Jae Sue Choi, Misung Kwon, Jiwoong Choi, Taisun Shin, Daeseok Byun, Jaeil Kim, Hyeung Rak KimAbstract:Ecklonia Stolonifera is a brown alga that was shown to have antioxidant, anti-inflammatory, tyrosinase inhibitory, and chemopreventive activities. However, the molecular mechanisms underlying its anti-inflammatory activity remain unclear. In this study, we investigated the molecular mechanism of the anti-inflammatory action of E. Stolonifera ethanolic extracts (ESE) using lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. ESE inhibited LPS-induced nitric oxide (IC50 = 72 ± 1.9 μg/mL) and prostaglandin E2 (IC50 = 98 ± 5.3 μg/mL) production in a dose-dependent manner and suppressed the expression of inducible nitric oxide synthase and cyclooxygenase-2 in RAW 264.7 cells. ESE also reduced the production of pro-inflammatory cytokines in LPS-stimulated RAW 264.7 cells. LPS-induced nuclear factor-κB (NF-κB) transcriptional activity and NF-κB translocation into the nucleus were significantly inhibited by ESE treatment through the prevention of the degradation of inhibitor κB-α. Moreover, ESE inhibited the acti...
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Quantitative determination of major phlorotannins in Ecklonia Stolonifera.
Archives of pharmacal research, 2010Co-Authors: Hyun Ryul Goo, Jae Sue ChoiAbstract:Ecklonia Stolonifera is a rich source of phlorotannins, which are responsible for the potent pharmacological effects associated with this seaweed. The purpose of this study was to develop a reversed-phase high-performance liquid chromatography method for the simultaneous determination of three major phlorotannins, eckol, dieckol, and phlorofucofuroeckol-A, in the extracts of Ecklonia Stolonifera. The optimal chromatographic conditions were achieved on a Thermo Hypersil Gold C-18 column (250 × 4.6 mm i.d., 5 μm) using linear gradient elution of acetonitrile and water containing 0.1% formic acid at UV 254 nm. The separated phlorotannins were identified by liquid chromatography-mass spectrometry. The high-performance liquid chromatography method showed good linearity (r2 > 0.998), precision (1.4–9.5%), and accuracy (93.9–108.7%). The limits of detection ranged from 0.06 to 0.30 μg/mL and the lower limits of quantitation ranged from 0.2 to 1.0 μg/mL. Among phlorotannins, dieckol was the most abundant in both ethanol and ethyl acetate extracts of Ecklonia Stolonifera.
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inhibitory effects of phloroglucinol derivatives isolated from ecklonia Stolonifera on fceri expression
Bioorganic & Medicinal Chemistry, 2009Co-Authors: Sunyup Shim, Jae Sue Choi, Daeseok ByunAbstract:Two bioactive phloroglucinol derivatives, dioxinodehydroeckol (DHE) and phlorofucofuroeckol A (PFF-A) were isolated from edible marine brown alga, Ecklonia Stolonifera, and evaluated for effects on cell surface Fc(epsilon)RI expression in KU812F cells. DHE and PFF-A were found to reduce the cell surface expression, and total cellular protein and mRNA levels for the Fc(epsilon)RI alpha chain. Moreover, both compounds exerted inhibitory effects against the elevation of intracellular calcium concentration [Ca(2+)](i) and histamine release from anti-Fc(epsilon)RI alpha chain antibody (CRA-1)-stimulated cells. These inhibitory effects were stronger for PFF-A than for DHE. These results show that two phloroglucinol derivatives, DHE and PFF-A, may exert anti-allergic effects via the inhibition of Fc(epsilon)RI expression, calcium influx, and degranulation in basophils, and contributes to the pharmacological activities of marine brown alga, including E. Stolonifera.
Hyeung Rak Kim - One of the best experts on this subject based on the ideXlab platform.
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isolation and identification of anti inflammatory compounds from ethyl acetate fraction of ecklonia Stolonifera and their anti inflammatory action
Journal of Applied Phycology, 2016Co-Authors: Ran Wei, Chang Geun Choi, Minsup Lee, Bonggi Lee, Hyeung Rak KimAbstract:Ecklonia Stolonifera, a brown alga, has been reported to have several biological activities, including antioxidation, anti-inflammation, and hepatoprotection. This study aims to investigate the anti-inflammatory effect of the ethyl acetate fraction of E. Stolonifera (ESA) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. ESA inhibited the production of LPS-induced nitric oxide (NO) and prostaglandin E2 and reduced the expression of inducible nitric oxide synthase and cyclooxygenase-2 in a dose-dependent manner. ESA also inhibited the production of pro-inflammatory cytokines, including tumor necrosis factor α, interleukin (IL)-1β, and IL-6. LPS-induced transcriptional activation of nuclear factor-κB was significantly suppressed by ESA through the prevention of inhibitor κB-α degradation. Eight phlorotannins, including 2-phloroeckol, dioxinodehydroeckol, eckol, phlorofucofuroeckol B, 6,6′-bieckol, dieckol, 974-B, and phlorofucofuroeckol A were isolated from ESA, and their chemical structures were elucidated by NMR spectroscopic analysis. Based on the inhibition of NO production in LPS-treated RAW 264.7 cells, the major anti-inflammatory components in ESA were identified as 2′-phloroeckol, 6,6′-bieckol, phlorofucofuroeckol A, phlorofucofuroeckol B, and 974-B. Our results indicate that E. Stolonifera may be considered as a therapeutic agent for the prevention of inflammatory disorders.
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anti inflammatory activities of an ethanol extract of ecklonia Stolonifera in lipopolysaccharide stimulated raw 264 7 murine macrophage cells
Journal of Agricultural and Food Chemistry, 2012Co-Authors: Minsup Lee, Jae Sue Choi, Misung Kwon, Jiwoong Choi, Taisun Shin, Daeseok Byun, Jaeil Kim, Hyeung Rak KimAbstract:Ecklonia Stolonifera is a brown alga that was shown to have antioxidant, anti-inflammatory, tyrosinase inhibitory, and chemopreventive activities. However, the molecular mechanisms underlying its anti-inflammatory activity remain unclear. In this study, we investigated the molecular mechanism of the anti-inflammatory action of E. Stolonifera ethanolic extracts (ESE) using lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. ESE inhibited LPS-induced nitric oxide (IC50 = 72 ± 1.9 μg/mL) and prostaglandin E2 (IC50 = 98 ± 5.3 μg/mL) production in a dose-dependent manner and suppressed the expression of inducible nitric oxide synthase and cyclooxygenase-2 in RAW 264.7 cells. ESE also reduced the production of pro-inflammatory cytokines in LPS-stimulated RAW 264.7 cells. LPS-induced nuclear factor-κB (NF-κB) transcriptional activity and NF-κB translocation into the nucleus were significantly inhibited by ESE treatment through the prevention of the degradation of inhibitor κB-α. Moreover, ESE inhibited the acti...
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acetyl and butyrylcholinesterase inhibitory activities of sterols and phlorotannins from ecklonia Stolonifera
Fisheries Science, 2008Co-Authors: Na Young Yoon, Hae Young Chung, Hyeung Rak Kim, Jae Sue ChoiAbstract:As part of this study on the isolation of cholinesterase inhibitors from natural marine products, the bioactivity of the ethanolic extracts from 27 Korean seaweeds were screened using acetylcholinesterase (AChE) and butyrylcholine sterase (BChE) inhibitory assays. Ecklonia Stolonifera exhibited promising inhibitory properties against both AChE and BChE. Bioassay-guided fractionation of the active n-hexane and ethyl acetate (EtOAc) soluble fractions, obtained from the ethanolic extract of E. Stolonifera, resulted in the isolation of the sterols; fucosterol (1) and 24-hydroperoxy 24-vinylcholesterol (2), from the n-hexane fraction and the phlorotannins; phloroglucinol (3), ecks-tolonol (4), eckol (5), phlorofucofuroeckol-A (6), dieckol (7), triphlorethol-A (8), 2-phloroeckol (9) and 7-phloroeckol (10), from the EtOAc fraction. Of these, compounds 2, 9 and 10 were isolated from E. Stolonifera for the first time. Compounds 4–7, 9 and 10 exhibited inhibitory potential against AChE, with 50% inhibition concentration (IC50) values of 42.66±8.48, 20.56±5,61, 4.89±2.28, 17.11±3.24, 38.13±4.95 and 21.11±4.16 μM, respectively; whereas, compounds 1, 2, 4 and 6 were found to be active against BChE, with IC50 values of 421.72±1.43, 176.46±2.51, 230.27±3.52 and 136.71±3.33 μM, respectively. It has been suggested that the inhibition of these enzymes by the sterols and phlorotannins derived from marine brown algae could be a useful approach for the treatment of Alzheimer’s disease.
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angiotensin converting enzyme i inhibitory activity of phlorotannins from ecklonia Stolonifera
Fisheries Science, 2006Co-Authors: Hyun Ah Jung, Hyeung Rak Kim, Sook Kyung Hyun, Jae Sue ChoiAbstract:As part of our study of the isolation of antihypertensive agents derived from natural marine products, the bioactivity of 10 edible Korean seaweeds were screened by angiotensin converting enzyme (ACE) inhibitory and peroxynitrite assays. Among the crude extracts of selected sea-weeds, including five Phaeophyta (Ecklonia Stolonifera, E. cava, Pelvetia siliquosa, Hizikia fusiforme, and Undaria pinnatifida), four Rhodophyta (Gigartina tenella, Gelidium amansii, Chondria crassicaulis, and Porphyra tenera) and one Chlorophyta (Capsosiphon fulvescens), the ethanol extracts of E. Stolonifera, E. cava, P. siliquosa, U. pinnatifida, and G. tenella exhibited significant inhibitory properties against ACE at more than 50% inhibition at a concentration of 163.93 μg/mL. Phloroglucinol 1, eckstolonol 2, eckol 3, phlorofucofuroeckol A 4, and dieckol 5 had been isolated previously, and triphlorethol-A 6 and fucosterol 7 were isolated for the first time from E. Stolonifera. Also, the ACE inhibitory and peroxynitrite scavenging properties of phlorotannins 1–6 were evaluated, along with fucosterol 7 obtained from E. Stolonifera. Among profound peroxynitrite scavenging compounds 1–6, phlorotannins 3, 4 and 5 were also determined to manifest marked inhibitory activity against ACE, with 50% inhibition concentration (IC50) values of 70.82±0.25, 12.74¯0.15, and 34.25±3.56 μM, respectively.
Marta Helena Fillet Spoto - One of the best experts on this subject based on the ideXlab platform.
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essential oils from eucalyptus staigeriana f muell ex bailey and eucalyptus urograndis w hill ex maiden associated to carboxymethylcellulose coating for the control of botrytis cinerea pers fr and rhizopus stolonifer ehrenb fr vuill in strawberries
Industrial Crops and Products, 2020Co-Authors: Paula Porrelli Moreira Da Silva, Jacqueline De Oliveira, Anaile Dos Mares Biazotto, Marise Martins Parisi, Eduardo Micoti Da Gloria, Marta Helena Fillet SpotoAbstract:Abstract The fungi Rhizopus stolonifer (Ehrenb.:Fr.) Vuill. (R. stolonifer (Ehrenb.:Fr.) Vuill.) and Botrytis cinerea Pers. Fr. (B. cinerea Pers. Fr.) are causal agents of important post-harvest diseases in strawberries. For their control, the essential oil (EO) extracted from the leaves of Eucalyptus staigeriana Muell. ex Bailey (E. staigeriana F. Muell. ex Bailey) and Eucalyptus urograndis W. Hill ex Maiden (E. urograndis W. Hill ex Maiden) can be a good alternative to the synthetic fungicides. Thus, the antifungal activity of the EOs from E. staigeriana F. Muell. ex Bailey and from E. urograndis W. Hill ex Maiden was evaluated in vitro on the fungi B. cinerea Pers. Fr. and R. stolonifer (Ehrenb.:Fr.) Vuill.. The effects on pathogen morphology and the in vivo antifungal activity of the EO that presented the highest activity in vitro, associated with carboxymethylcellulose (CMC), were also verified. The chemical composition of the essential oils evaluated was determined. The EO from E. staigeriana F. Muell. ex Bailey was the most efficient in the in vitro assays on the two fungi. This EO, whose major compound is limonene, caused dehydration and rupture of the fungal hyphae, suggesting its action is on the cell wall and membrane. The incorporation of this EO to CMC in the curative way reduced the severity of soft rot, caused by R. stolonifer (Ehrenb.:Fr.) Vuill.; nevertheless, it did not present an effect on the inhibition of B. cinerea Pers. Fr.. These results suggest that E. staigeriana F. Muell. ex Bailey EO, associated to CMC coating, can be an alternative to the use of synthetic fungicides for the control of R. stolonifer (Ehrenb.:Fr.) Vuill., mainly.
Jinhyeuk Kwon - One of the best experts on this subject based on the ideXlab platform.
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occurrence of rhizopus soft rot on rubus crataegifolius caused by rhizopus stolonifer in korea
Research in Plant Disease, 2008Co-Authors: Jinhyeuk KwonAbstract:Rhizopus soft rot caused by Rhizopus stolonifer on June, 2008 occurred on Rubus crataegifolius Bunge in the box at Jinju City Wholesale Market of Agricultural Products at Gyeongnam province in Korea. The infected fruits were rapidly water-soaked, softened, black and eventually rotted. The symptoms occurred after infecting wounds at harvest time. The colony were white to light brown, formed much sporangiospores. The optimum temperature of the fungus on potato dextrose agar was . Sporangiophores were in width. Sporangia were globose or hemispheric, white at first and gradually to black, and in size. Columella were hemispheric, and in size. Sporangiospores were irregular round or oval, brownishblack and in size. On the basis of symptom, mycological characteristics and pathogenicity of the fungus, the causal fungus was identified as Rhizopus stolonifer. This is the first report of Rhizopus soft rot by R. stolonifer on R. crataegifolius in Korea.
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occurrence of rhizopus soft rot on apple fruit caused by rhizopus stolonifer in korea
Research in Plant Disease, 2008Co-Authors: Jinhyeuk KwonAbstract:A rhizopus soft rot caused by Rhizopus stolonifer occurred sporadically on apple fruits (Malus pumila var. dulcissima Koidz.) at a wholesale market of agricultural products in Jinju, Korea in 2006 and 2007. Infected fruits were rapidly water-soaked, softened and rotted. The symptoms were initiated mainly from wounds or cracks occurred at harvest time. Sporangiophores were in length and in width. Sporangia were globose or hemispheric and in size. The color of sporangia was white and cottony at first and gradually turned to brownish black, bearing abundant spores when matured. Columella were hemispheric and in size. Sporangiospores were irregular round or oval, brownish-black streaked and in size. Optimum temperature for mycelial growth of the fungus on PDA was . On the basis of mycological characteristics and pathogenicity to apple the causal fungus was identified as Rhizopus stolonifer (Ehrenb.) Vuill. This is the first report of Rhizopus soft rot by R. stolonifer on Fuji apple in Korea.
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occurrence of rhizopus soft rot on apple fruit caused by rhizopus stolonifer in korea
Research in Plant Disease, 2008Co-Authors: Jinhyeuk KwonAbstract:A rhizopus soft rot caused by Rhizopus stolonifer occurred sporadically on apple fruits (Malus pumila var. dulcissima Koidz.) at a wholesale market of agricultural products in Jinju, Korea in 2006 and 2007. Infected fruits were rapidly water-soaked, softened and rotted. The symptoms were initiated mainly from wounds or cracks occurred at harvest time. Sporangiophores were in length and in width. Sporangia were globose or hemispheric and in size. The color of sporangia was white and cottony at first and gradually turned to brownish black, bearing abundant spores when matured. Columella were hemispheric and in size. Sporangiospores were irregular round or oval, brownish-black streaked and in size. Optimum temperature for mycelial growth of the fungus on PDA was . On the basis of mycological characteristics and pathogenicity to apple the causal fungus was identified as Rhizopus stolonifer (Ehrenb.) Vuill. This is the first report of Rhizopus soft rot by R. stolonifer on Fuji apple in Korea.
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occurrence of rhizopus soft rot on musa sapientum caused by rhizopus stolonifer in korea
The Korean Journal of Mycology, 2007Co-Authors: Jinhyeuk KwonAbstract:Rhizopus soft rot caused by Rhizopus stolonifer occurred on banana (Musa sapientum) in the box at Jinju City Wholesale Market of Agricultural Products at Gyeongnam province in Korea. The infected fruits were rapidly water-soaked, softened, blackened and eventually rotted. They were infected from wounds at harvest time. The colony of the isolate from the infected fruit was white to light brown and formed many sporangiospores. The optimum growth temperature of the fungus on potato dextrose agar was . Sporangiophores were in width. Sporangia were globose or hemispheric, white at first and changed gradually to black, and in size. Columella were hemispheric, and in size. Sporangiospores were irregular round or oval, brownish-black and in size. On the basis of symptom, mycological characteristics and pathogenicity to banana, the causal fungus was identified as Rhizopus stolonifer. This is the first report of Rhizopus soft rot by R. stolonifer on banana in Korea.
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rhizopus soft rot on pear pyrus serotina caused by rhizopus stolonifer in korea
Mycobiology, 2006Co-Authors: Jinhyeuk KwonAbstract:Rhizopus soft rot caused by Rhizopus stolonifer occurred on pears (Pyrus serotina) in the Jinju City Agricultural Products Wholesale Market in Korea from 2004 to 2005. The infection usually started from wounds due to cracking at harvest time. The lesions started as water-soaked, rapidly softened, then gradually expanded. The mycelia grew vigorously on the surface of the fruits and formed stolons. Colonies on potato dextrose agar at 25℃ were white cottony to brownish black. Sporangia were globose, black and 90~120 µm in size. Sporangiophores were light brown and 480~2600 × 12~18 µm in size. Sporangiospores were globose to oval, brownish, streaked, and 8~14 × 6~10 µm in size. Columella were light brownish gray, hemispherical and 70~80 µm in size. On the basis of these symptoms, mycological characteristics and pathogenicity tests on host plants, the fungus was identified as Rhizopus stolonifer (Ehrenb.) Vuill. This is the first report of rhizopus soft rot on pear (P. serotina) caused by R. stolonifer in Korea.
