The Experts below are selected from a list of 300 Experts worldwide ranked by ideXlab platform

Akira Kurisaki - One of the best experts on this subject based on the ideXlab platform.

  • formation of Stomach Tissue by organoid culture using mouse embryonic stem cells
    Methods of Molecular Biology, 2017
    Co-Authors: Takaaki K Noguchi, Akira Kurisaki
    Abstract:

    : In this chapter, we describe a method for the induction of Stomach organoids from mouse embryonic stem (ES) cells. We used an embryoid body-based differentiation method to induce gastric primordial epithelium covered with mesenchyme and further differentiate it in Matrigel by 3D culture. The differentiated organoid contains both corpus- and antrum-specific mature gastric Tissue cells. This protocol may be useful for a variety of studies in developmental biology and disease modeling of the Stomach.

  • generation of Stomach Tissue from mouse embryonic stem cells
    Nature Cell Biology, 2015
    Co-Authors: Takaaki K Noguchi, Naoto Ninomiya, Mari Sekine, Shinji Komazaki, Pichao Wang, Makoto Asashima, Akira Kurisaki
    Abstract:

    Successful pluripotent stem cell differentiation methods have been developed for several endoderm-derived cells, including hepatocytes, β-cells and intestinal cells. However, Stomach lineage commitment from pluripotent stem cells has remained a challenge, and only antrum specification has been demonstrated. We established a method for Stomach differentiation from embryonic stem cells by inducing mesenchymal Barx1, an essential gene for in vivo Stomach specification from gut endoderm. Barx1-inducing culture conditions generated Stomach primordium-like spheroids, which differentiated into mature Stomach Tissue cells in both the corpus and antrum by three-dimensional culture. This embryonic stem cell-derived Stomach Tissue (e-ST) shared a similar gene expression profile with adult Stomach, and secreted pepsinogen as well as gastric acid. Furthermore, TGFA overexpression in e-ST caused hypertrophic mucus and gastric anacidity, which mimicked Menetrier disease in vitro. Thus, in vitro Stomach Tissue derived from pluripotent stem cells mimics in vivo development and can be used for Stomach disease models.

  • Generation of Stomach Tissue from mouse embryonic stem cells
    Nature Cell Biology, 2015
    Co-Authors: Takaaki K Noguchi, Naoto Ninomiya, Mari Sekine, Shinji Komazaki, Pichao Wang, Makoto Asashima, Akira Kurisaki
    Abstract:

    Noguchi and colleagues report the generation of Stomach-like Tissue from mouse embryonic stem cells. They show that the Tissue contains all Stomach-specific cell types and secretes acid and digestive enzyme. Successful pluripotent stem cell differentiation methods have been developed for several endoderm-derived cells, including hepatocytes, β-cells and intestinal cells. However, Stomach lineage commitment from pluripotent stem cells has remained a challenge, and only antrum specification has been demonstrated. We established a method for Stomach differentiation from embryonic stem cells by inducing mesenchymal Barx1 , an essential gene for in vivo Stomach specification from gut endoderm. Barx1 -inducing culture conditions generated Stomach primordium-like spheroids, which differentiated into mature Stomach Tissue cells in both the corpus and antrum by three-dimensional culture. This embryonic stem cell-derived Stomach Tissue (e-ST) shared a similar gene expression profile with adult Stomach, and secreted pepsinogen as well as gastric acid. Furthermore, TGFA overexpression in e-ST caused hypertrophic mucus and gastric anacidity, which mimicked Ménétrier disease in vitro . Thus, in vitro Stomach Tissue derived from pluripotent stem cells mimics in vivo development and can be used for Stomach disease models.

Takaaki K Noguchi - One of the best experts on this subject based on the ideXlab platform.

  • formation of Stomach Tissue by organoid culture using mouse embryonic stem cells
    Methods of Molecular Biology, 2017
    Co-Authors: Takaaki K Noguchi, Akira Kurisaki
    Abstract:

    : In this chapter, we describe a method for the induction of Stomach organoids from mouse embryonic stem (ES) cells. We used an embryoid body-based differentiation method to induce gastric primordial epithelium covered with mesenchyme and further differentiate it in Matrigel by 3D culture. The differentiated organoid contains both corpus- and antrum-specific mature gastric Tissue cells. This protocol may be useful for a variety of studies in developmental biology and disease modeling of the Stomach.

  • generation of Stomach Tissue from mouse embryonic stem cells
    Nature Cell Biology, 2015
    Co-Authors: Takaaki K Noguchi, Naoto Ninomiya, Mari Sekine, Shinji Komazaki, Pichao Wang, Makoto Asashima, Akira Kurisaki
    Abstract:

    Successful pluripotent stem cell differentiation methods have been developed for several endoderm-derived cells, including hepatocytes, β-cells and intestinal cells. However, Stomach lineage commitment from pluripotent stem cells has remained a challenge, and only antrum specification has been demonstrated. We established a method for Stomach differentiation from embryonic stem cells by inducing mesenchymal Barx1, an essential gene for in vivo Stomach specification from gut endoderm. Barx1-inducing culture conditions generated Stomach primordium-like spheroids, which differentiated into mature Stomach Tissue cells in both the corpus and antrum by three-dimensional culture. This embryonic stem cell-derived Stomach Tissue (e-ST) shared a similar gene expression profile with adult Stomach, and secreted pepsinogen as well as gastric acid. Furthermore, TGFA overexpression in e-ST caused hypertrophic mucus and gastric anacidity, which mimicked Menetrier disease in vitro. Thus, in vitro Stomach Tissue derived from pluripotent stem cells mimics in vivo development and can be used for Stomach disease models.

  • Generation of Stomach Tissue from mouse embryonic stem cells
    Nature Cell Biology, 2015
    Co-Authors: Takaaki K Noguchi, Naoto Ninomiya, Mari Sekine, Shinji Komazaki, Pichao Wang, Makoto Asashima, Akira Kurisaki
    Abstract:

    Noguchi and colleagues report the generation of Stomach-like Tissue from mouse embryonic stem cells. They show that the Tissue contains all Stomach-specific cell types and secretes acid and digestive enzyme. Successful pluripotent stem cell differentiation methods have been developed for several endoderm-derived cells, including hepatocytes, β-cells and intestinal cells. However, Stomach lineage commitment from pluripotent stem cells has remained a challenge, and only antrum specification has been demonstrated. We established a method for Stomach differentiation from embryonic stem cells by inducing mesenchymal Barx1 , an essential gene for in vivo Stomach specification from gut endoderm. Barx1 -inducing culture conditions generated Stomach primordium-like spheroids, which differentiated into mature Stomach Tissue cells in both the corpus and antrum by three-dimensional culture. This embryonic stem cell-derived Stomach Tissue (e-ST) shared a similar gene expression profile with adult Stomach, and secreted pepsinogen as well as gastric acid. Furthermore, TGFA overexpression in e-ST caused hypertrophic mucus and gastric anacidity, which mimicked Ménétrier disease in vitro . Thus, in vitro Stomach Tissue derived from pluripotent stem cells mimics in vivo development and can be used for Stomach disease models.

Naoto Ninomiya - One of the best experts on this subject based on the ideXlab platform.

  • generation of Stomach Tissue from mouse embryonic stem cells
    Nature Cell Biology, 2015
    Co-Authors: Takaaki K Noguchi, Naoto Ninomiya, Mari Sekine, Shinji Komazaki, Pichao Wang, Makoto Asashima, Akira Kurisaki
    Abstract:

    Successful pluripotent stem cell differentiation methods have been developed for several endoderm-derived cells, including hepatocytes, β-cells and intestinal cells. However, Stomach lineage commitment from pluripotent stem cells has remained a challenge, and only antrum specification has been demonstrated. We established a method for Stomach differentiation from embryonic stem cells by inducing mesenchymal Barx1, an essential gene for in vivo Stomach specification from gut endoderm. Barx1-inducing culture conditions generated Stomach primordium-like spheroids, which differentiated into mature Stomach Tissue cells in both the corpus and antrum by three-dimensional culture. This embryonic stem cell-derived Stomach Tissue (e-ST) shared a similar gene expression profile with adult Stomach, and secreted pepsinogen as well as gastric acid. Furthermore, TGFA overexpression in e-ST caused hypertrophic mucus and gastric anacidity, which mimicked Menetrier disease in vitro. Thus, in vitro Stomach Tissue derived from pluripotent stem cells mimics in vivo development and can be used for Stomach disease models.

  • Generation of Stomach Tissue from mouse embryonic stem cells
    Nature Cell Biology, 2015
    Co-Authors: Takaaki K Noguchi, Naoto Ninomiya, Mari Sekine, Shinji Komazaki, Pichao Wang, Makoto Asashima, Akira Kurisaki
    Abstract:

    Noguchi and colleagues report the generation of Stomach-like Tissue from mouse embryonic stem cells. They show that the Tissue contains all Stomach-specific cell types and secretes acid and digestive enzyme. Successful pluripotent stem cell differentiation methods have been developed for several endoderm-derived cells, including hepatocytes, β-cells and intestinal cells. However, Stomach lineage commitment from pluripotent stem cells has remained a challenge, and only antrum specification has been demonstrated. We established a method for Stomach differentiation from embryonic stem cells by inducing mesenchymal Barx1 , an essential gene for in vivo Stomach specification from gut endoderm. Barx1 -inducing culture conditions generated Stomach primordium-like spheroids, which differentiated into mature Stomach Tissue cells in both the corpus and antrum by three-dimensional culture. This embryonic stem cell-derived Stomach Tissue (e-ST) shared a similar gene expression profile with adult Stomach, and secreted pepsinogen as well as gastric acid. Furthermore, TGFA overexpression in e-ST caused hypertrophic mucus and gastric anacidity, which mimicked Ménétrier disease in vitro . Thus, in vitro Stomach Tissue derived from pluripotent stem cells mimics in vivo development and can be used for Stomach disease models.

Mari Sekine - One of the best experts on this subject based on the ideXlab platform.

  • generation of Stomach Tissue from mouse embryonic stem cells
    Nature Cell Biology, 2015
    Co-Authors: Takaaki K Noguchi, Naoto Ninomiya, Mari Sekine, Shinji Komazaki, Pichao Wang, Makoto Asashima, Akira Kurisaki
    Abstract:

    Successful pluripotent stem cell differentiation methods have been developed for several endoderm-derived cells, including hepatocytes, β-cells and intestinal cells. However, Stomach lineage commitment from pluripotent stem cells has remained a challenge, and only antrum specification has been demonstrated. We established a method for Stomach differentiation from embryonic stem cells by inducing mesenchymal Barx1, an essential gene for in vivo Stomach specification from gut endoderm. Barx1-inducing culture conditions generated Stomach primordium-like spheroids, which differentiated into mature Stomach Tissue cells in both the corpus and antrum by three-dimensional culture. This embryonic stem cell-derived Stomach Tissue (e-ST) shared a similar gene expression profile with adult Stomach, and secreted pepsinogen as well as gastric acid. Furthermore, TGFA overexpression in e-ST caused hypertrophic mucus and gastric anacidity, which mimicked Menetrier disease in vitro. Thus, in vitro Stomach Tissue derived from pluripotent stem cells mimics in vivo development and can be used for Stomach disease models.

  • Generation of Stomach Tissue from mouse embryonic stem cells
    Nature Cell Biology, 2015
    Co-Authors: Takaaki K Noguchi, Naoto Ninomiya, Mari Sekine, Shinji Komazaki, Pichao Wang, Makoto Asashima, Akira Kurisaki
    Abstract:

    Noguchi and colleagues report the generation of Stomach-like Tissue from mouse embryonic stem cells. They show that the Tissue contains all Stomach-specific cell types and secretes acid and digestive enzyme. Successful pluripotent stem cell differentiation methods have been developed for several endoderm-derived cells, including hepatocytes, β-cells and intestinal cells. However, Stomach lineage commitment from pluripotent stem cells has remained a challenge, and only antrum specification has been demonstrated. We established a method for Stomach differentiation from embryonic stem cells by inducing mesenchymal Barx1 , an essential gene for in vivo Stomach specification from gut endoderm. Barx1 -inducing culture conditions generated Stomach primordium-like spheroids, which differentiated into mature Stomach Tissue cells in both the corpus and antrum by three-dimensional culture. This embryonic stem cell-derived Stomach Tissue (e-ST) shared a similar gene expression profile with adult Stomach, and secreted pepsinogen as well as gastric acid. Furthermore, TGFA overexpression in e-ST caused hypertrophic mucus and gastric anacidity, which mimicked Ménétrier disease in vitro . Thus, in vitro Stomach Tissue derived from pluripotent stem cells mimics in vivo development and can be used for Stomach disease models.

Shinji Komazaki - One of the best experts on this subject based on the ideXlab platform.

  • generation of Stomach Tissue from mouse embryonic stem cells
    Nature Cell Biology, 2015
    Co-Authors: Takaaki K Noguchi, Naoto Ninomiya, Mari Sekine, Shinji Komazaki, Pichao Wang, Makoto Asashima, Akira Kurisaki
    Abstract:

    Successful pluripotent stem cell differentiation methods have been developed for several endoderm-derived cells, including hepatocytes, β-cells and intestinal cells. However, Stomach lineage commitment from pluripotent stem cells has remained a challenge, and only antrum specification has been demonstrated. We established a method for Stomach differentiation from embryonic stem cells by inducing mesenchymal Barx1, an essential gene for in vivo Stomach specification from gut endoderm. Barx1-inducing culture conditions generated Stomach primordium-like spheroids, which differentiated into mature Stomach Tissue cells in both the corpus and antrum by three-dimensional culture. This embryonic stem cell-derived Stomach Tissue (e-ST) shared a similar gene expression profile with adult Stomach, and secreted pepsinogen as well as gastric acid. Furthermore, TGFA overexpression in e-ST caused hypertrophic mucus and gastric anacidity, which mimicked Menetrier disease in vitro. Thus, in vitro Stomach Tissue derived from pluripotent stem cells mimics in vivo development and can be used for Stomach disease models.

  • Generation of Stomach Tissue from mouse embryonic stem cells
    Nature Cell Biology, 2015
    Co-Authors: Takaaki K Noguchi, Naoto Ninomiya, Mari Sekine, Shinji Komazaki, Pichao Wang, Makoto Asashima, Akira Kurisaki
    Abstract:

    Noguchi and colleagues report the generation of Stomach-like Tissue from mouse embryonic stem cells. They show that the Tissue contains all Stomach-specific cell types and secretes acid and digestive enzyme. Successful pluripotent stem cell differentiation methods have been developed for several endoderm-derived cells, including hepatocytes, β-cells and intestinal cells. However, Stomach lineage commitment from pluripotent stem cells has remained a challenge, and only antrum specification has been demonstrated. We established a method for Stomach differentiation from embryonic stem cells by inducing mesenchymal Barx1 , an essential gene for in vivo Stomach specification from gut endoderm. Barx1 -inducing culture conditions generated Stomach primordium-like spheroids, which differentiated into mature Stomach Tissue cells in both the corpus and antrum by three-dimensional culture. This embryonic stem cell-derived Stomach Tissue (e-ST) shared a similar gene expression profile with adult Stomach, and secreted pepsinogen as well as gastric acid. Furthermore, TGFA overexpression in e-ST caused hypertrophic mucus and gastric anacidity, which mimicked Ménétrier disease in vitro . Thus, in vitro Stomach Tissue derived from pluripotent stem cells mimics in vivo development and can be used for Stomach disease models.