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Poels, Lambert G. - One of the best experts on this subject based on the ideXlab platform.

Jennifer L. Hunt - One of the best experts on this subject based on the ideXlab platform.

  • Ductal adenomas of salivary gland showing features of Striated Duct differentiation ('Striated Duct adenoma'): a report of six cases.
    Histopathology, 2010
    Co-Authors: Ilan Weinreb, Roderick H.w. Simpson, Alena Skálová, Bayardo Perez-ordonez, Irving Dardick, Runjan Chetty, Jennifer L. Hunt
    Abstract:

    Weinreb I, Simpson R H, Skalova A, Perez-Ordonez B, Dardick I, Chetty R & Hunt J L (2010) Histopathology 57, 707–715 Ductal adenomas of salivary gland showing features of Striated Duct differentiation (‘Striated Duct adenoma’): a report of six cases Aims:  To describe a salivary adenoma composed largely of unilayered Ducts resembling Striated Ducts, and to differentiate it from similar adenomas, including canalicular and intercalated Duct adenoma. Methods and results:  Six unilayered Ductal adenomas were identified in parotid (four) and palate (two). They were encapsulated, ranged from 0.5 to 3.0 cm and composed of closely apposed Ducts with virtually no stroma. The Ducts varied in size and showed cysts up to 0.1 cm. The cells were eosinophilic and bland. Prominent cell membranes, reminiscent of ‘striations’ of normal Striated Ducts, were seen. The typical epithelial ‘beading’ pattern with abundant stroma of canalicular adenoma was absent. The tumours expressed keratins (six of six) and S100 (five of six). Smooth muscle actin (SMA) revealed no myoepithelial cells. Two tumours showed focal bilayered Ducts with calponin or smooth muscle myosin heavy chain, but the tumours were largely unilayered. Only isolated cells in three tumours were positive with p63; a pattern identical to Striated Ducts. In contrast, the normal excretory and intercalated Ducts all contained diffuse bilayering with basal or myoepithelial markers. Conclusions:  Striated Duct adenomas are unilayered Ductal tumours that recapitulate normal Striated Ducts.

Kazuo Hosoi - One of the best experts on this subject based on the ideXlab platform.

  • inDuction of sca 1 in the Duct cells of the mouse submandibular gland by obstruction of the main excretory Duct
    Journal of Oral Pathology & Medicine, 2011
    Co-Authors: Nunuk Purwanti, Daisuke Tsuji, Ahmad Azlina, Mileva Ratko Karabasil, Purevjav Javkhlan, Takahiro Hasegawa, Chenjuan Yao, Tetsuya Akamatsu, Kohji Itoh, Kazuo Hosoi
    Abstract:

    The effect of ligation of the main excretory Duct (MED) of the mouse submandibular gland (SMG) on the expression of Sca-1, a stem cell antigen, was examined by Western blotting and immunohistochemistry. By Western blotting, the expression of Sca-1 with a molecular weight of 18 kDa was identified in the normal gland. At 1 day post-ligation, the expression level of Sca-1 was strongly increased in the experimental gland and weakly in the contralateral gland, and such expression in both glands decreased at 6 days. By immunohistochemistry, Sca-1 was detected weakly in the apical membrane of excretory Duct (ED) cells of the SMG under the normal condition. By Duct ligation, Sca-1 became expressed strongly in most cells of the two major Duct systems, i.e., the Striated Duct (SD) and granular convoluted tubules (GCT), but was not detected in the acinar (Ac) cells. By fluorescence-activated cell sorter (FACS) analysis, the number of side population (SP) cells in this gland was found to be increased by ligation. These results imply that Sca-1-positive cells may have a role in the Duct cell proliferation in the regeneration step elicited by MED ligation-induced injury.

  • immunocytochemical study of granular Duct cells with a hormonally enhanced granular cell phenotype in the mouse parotid gland
    Odontology, 2009
    Co-Authors: Shingo Kurabuchi, Kazuo Hosoi
    Abstract:

    In the parotid glands (PGs) of intact male mice (12 weeks of age, ICR strain), immunofluorescence labels for a true tissue kallikrein, mK1, and for nerve growth factor (NGF) were recognized through the subluminal edges of the Striated Duct (SD) segments and interlobular Duct segments. Because of their small size, secretory granules were not detectable by light microscopy in any of the Duct cells. Full-fledged granular cells, containing large secretory granules that were visible by light microscopy, were induced in the SD segments of male mice after the injection of 5α-dehydrotestosterone (DHT) and triiodothyronine (T3), given either alone or in combination every other day for 2 weeks. A stronger effect was detected in the mice that were concomitantly injected with DHT and T3, and more abundant, fully developed granular cells appeared in the SD segments of these mice. These full-fledged granular cells were immunoreactive for mK1, NGF, and epidermal growth factor, but not for renin. The present results indicate that some of the SD cells with small granules in the mouse PG can develop a granular cell phenotype, producing more kinds of growth factors, as a result of the actions of androgen and thyroid hormone.

P Nieuwenhuis - One of the best experts on this subject based on the ideXlab platform.

  • rat cytomegalovirus replication in the salivary glands is exclusively confined to Striated Duct cells
    Virchows Archiv, 2000
    Co-Authors: J S Kloover, Janluuk Hillebrands, G De Wit, Gert Grauls, Jan Rozing, Cathrien A Bruggeman, P Nieuwenhuis
    Abstract:

    The salivary gland is the preferred organ for cytomegalovirus (CMV) replication and viral persistence. In order to identify the nature of infected cells and to study viral replication in more detail, several experiments were conDucted. Using the rat CMV (RCMV) model, acutely infected young adult rats (6 weeks of age) and new-born rats (3 days of age) were infected, and submandibular, parotid and sublingual glands were harvested at different time points after infection. For identification of the nature of infected cells, immunohistochemistry, in situ hybridisation and electron microscopic techniques were used. In young adult animals, the submandibular gland was the preferred organ for RCMV replication. The parotid and sublingual glands contained fewer viruses than the submandibular gland. In contrast, in new-born rats, the main site of RCMV replication was the sublingual gland, while the submandibular and parotid glands contained low amounts of virus. No virus could be detected in the parotid glands. In all glands of RCMV-infected animals, the infection was exclusively confined to Striated Duct cells. Infection resulted in a cellular inflammatory response which was mostly located in the interlobular Duct region, whereas only few inflammatory cells were found in the neighbourhood of infected Striated Duct cells. This phenomenon may contribute to the long persistence of the virus in this organ.

J K C Chan - One of the best experts on this subject based on the ideXlab platform.

  • lipoadenoma of the parotid gland with probable Striated Duct differentiation
    Modern Pathology, 1997
    Co-Authors: K C Yau, W Y W Tsang, J K C Chan
    Abstract:

    We report an example of lipoadenoma of the parotid gland, a previously undescribed tumor of the salivary gland. It is a well-circumscribed tumor comprising an intimate admixture of mature fat cells and branching narrow tubules. The tubules were elongated and had small or absent lumina, resulting in a remarkable Sertoliform pattern. They were lined by columnar cells, which were supported by a basal cell layer. The latter cells lacked evidence of myoepithelial differentiation but were best highlighted by immunostaining for high molecular-weight cytokeratin (34 beta E12). Comparison with the normal salivary gland components showed the greatest homology of the neoplastic tubules with the Striated Duct, which normally has an incomplete basal cell layer. The latter phenomenon is under-recognized, with some current textbooks still describing the presence of a single cell type only in the Striated Duct of the salivary gland.