Subsequent Biofilm Formation

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Xianghong Wang - One of the best experts on this subject based on the ideXlab platform.

  • Integrated antifouling and bactericidal polymer membranes through bioinspired polydopamine/poly(N-vinyl pyrrolidone) coating
    Applied Surface Science, 2016
    Co-Authors: Xianghong Wang, Shuaishuai Yuan, Yingkui Yang, Tao Jiang, Shifang Luan
    Abstract:

    Abstract Polypropylene (PP) non-woven has been widely used as wound dressing; however, the hydrophobic nature of PP can initiate bacterial attachment and Subsequent Biofilm Formation. Herein, we propose a facile approach to functionalize PP non-woven with poly(ethylene glycol) (PEG) and poly(N-vinyl pyrrolidone)-iodine complex (PVP-I). PVP and PEG were successively tethered onto PP non-woven surface via versatile bioinspired dopamine (DA) chemistry, followed by complexing iodine with PVP moieties. It was demonstrated through the field emission scanning electron microscope (SEM) and spread plate method that the as-modified PP non-woven integrated both antifouling property of PEG for suppressing bacterial adhesion, and bactericidal property of PVP-I for killing the few adherent bacteria. Meanwhile, it could greatly resist platelet and red blood cell adhesion. The integrated antifouling and bactericidal PP non-woven surfaces might have great potential in various wound dressing applications.

  • integrated antifouling and bactericidal polymer membranes through bioinspired polydopamine poly n vinyl pyrrolidone coating
    Applied Surface Science, 2016
    Co-Authors: Shuaishuai Yuan, Xianghong Wang, Yingkui Yang, Tao Jiang, Shifang Luan
    Abstract:

    Abstract Polypropylene (PP) non-woven has been widely used as wound dressing; however, the hydrophobic nature of PP can initiate bacterial attachment and Subsequent Biofilm Formation. Herein, we propose a facile approach to functionalize PP non-woven with poly(ethylene glycol) (PEG) and poly(N-vinyl pyrrolidone)-iodine complex (PVP-I). PVP and PEG were successively tethered onto PP non-woven surface via versatile bioinspired dopamine (DA) chemistry, followed by complexing iodine with PVP moieties. It was demonstrated through the field emission scanning electron microscope (SEM) and spread plate method that the as-modified PP non-woven integrated both antifouling property of PEG for suppressing bacterial adhesion, and bactericidal property of PVP-I for killing the few adherent bacteria. Meanwhile, it could greatly resist platelet and red blood cell adhesion. The integrated antifouling and bactericidal PP non-woven surfaces might have great potential in various wound dressing applications.

Shifang Luan - One of the best experts on this subject based on the ideXlab platform.

  • Integrated antifouling and bactericidal polymer membranes through bioinspired polydopamine/poly(N-vinyl pyrrolidone) coating
    Applied Surface Science, 2016
    Co-Authors: Xianghong Wang, Shuaishuai Yuan, Yingkui Yang, Tao Jiang, Shifang Luan
    Abstract:

    Abstract Polypropylene (PP) non-woven has been widely used as wound dressing; however, the hydrophobic nature of PP can initiate bacterial attachment and Subsequent Biofilm Formation. Herein, we propose a facile approach to functionalize PP non-woven with poly(ethylene glycol) (PEG) and poly(N-vinyl pyrrolidone)-iodine complex (PVP-I). PVP and PEG were successively tethered onto PP non-woven surface via versatile bioinspired dopamine (DA) chemistry, followed by complexing iodine with PVP moieties. It was demonstrated through the field emission scanning electron microscope (SEM) and spread plate method that the as-modified PP non-woven integrated both antifouling property of PEG for suppressing bacterial adhesion, and bactericidal property of PVP-I for killing the few adherent bacteria. Meanwhile, it could greatly resist platelet and red blood cell adhesion. The integrated antifouling and bactericidal PP non-woven surfaces might have great potential in various wound dressing applications.

  • integrated antifouling and bactericidal polymer membranes through bioinspired polydopamine poly n vinyl pyrrolidone coating
    Applied Surface Science, 2016
    Co-Authors: Shuaishuai Yuan, Xianghong Wang, Yingkui Yang, Tao Jiang, Shifang Luan
    Abstract:

    Abstract Polypropylene (PP) non-woven has been widely used as wound dressing; however, the hydrophobic nature of PP can initiate bacterial attachment and Subsequent Biofilm Formation. Herein, we propose a facile approach to functionalize PP non-woven with poly(ethylene glycol) (PEG) and poly(N-vinyl pyrrolidone)-iodine complex (PVP-I). PVP and PEG were successively tethered onto PP non-woven surface via versatile bioinspired dopamine (DA) chemistry, followed by complexing iodine with PVP moieties. It was demonstrated through the field emission scanning electron microscope (SEM) and spread plate method that the as-modified PP non-woven integrated both antifouling property of PEG for suppressing bacterial adhesion, and bactericidal property of PVP-I for killing the few adherent bacteria. Meanwhile, it could greatly resist platelet and red blood cell adhesion. The integrated antifouling and bactericidal PP non-woven surfaces might have great potential in various wound dressing applications.

Giuseppe Bisignano - One of the best experts on this subject based on the ideXlab platform.

  • Effect of alkaline pH on staphylococcal Biofilm Formation
    APMIS, 2012
    Co-Authors: Antonia Nostro, Anthony J Favaloro, Manuela D'arrigo, Anna Rita Blanco, Giuseppina Cutroneo, Luigina Cellini, Massimiliano Di Giulio, Andreana Marino, Giuseppe Bisignano
    Abstract:

    Biofilms are a serious problem, cause of severe inconvenience in the biomedical, food and industrial environment. Staphylococcus aureus and S. epidermidis are important pathogenic bacteria able to form thick and resistant Biofilms on various surfaces. Therefore, strategies aimed at preventing or at least interfering with the initial adhesion and Subsequent Biofilm Formation are a considerable achievement. The aim of this study was to evaluate the effect of alkaline pH on bacterial adhesion and further Biofilm Formation of S. aureus and S. epidermidis strains by Biofilm biomass, cell-surface hydrophobicity, scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) analysis. The results demonstrated that the amount of Biofilm biomass formed and the surface hydrophobicity were significantly less than what were observed at higher levels of pH. SEM and CLSM images revealed a poorly structured and very thin Biofilm (2.5-3 times thinner than that of the controls). The inhibiting effect of the alkaline pH on the bacterial attachment impaired the normal development of Biofilm that arrested at the microcolony stage. Alkaline formulations could be promising towards the control of bacterial colonization and therefore the reduction of the Biofilm-related hazard. In the clinical setting, alkaline solutions or cleaners could be promising to prevent the bacterial colonization, by treating surfaces such as catheters or indwelling medical devices, reducing the risk of Biofilm related infections.

Antonia Nostro - One of the best experts on this subject based on the ideXlab platform.

  • Effect of alkaline pH on staphylococcal Biofilm Formation
    APMIS, 2012
    Co-Authors: Antonia Nostro, Anthony J Favaloro, Manuela D'arrigo, Anna Rita Blanco, Giuseppina Cutroneo, Luigina Cellini, Massimiliano Di Giulio, Andreana Marino, Giuseppe Bisignano
    Abstract:

    Biofilms are a serious problem, cause of severe inconvenience in the biomedical, food and industrial environment. Staphylococcus aureus and S. epidermidis are important pathogenic bacteria able to form thick and resistant Biofilms on various surfaces. Therefore, strategies aimed at preventing or at least interfering with the initial adhesion and Subsequent Biofilm Formation are a considerable achievement. The aim of this study was to evaluate the effect of alkaline pH on bacterial adhesion and further Biofilm Formation of S. aureus and S. epidermidis strains by Biofilm biomass, cell-surface hydrophobicity, scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) analysis. The results demonstrated that the amount of Biofilm biomass formed and the surface hydrophobicity were significantly less than what were observed at higher levels of pH. SEM and CLSM images revealed a poorly structured and very thin Biofilm (2.5-3 times thinner than that of the controls). The inhibiting effect of the alkaline pH on the bacterial attachment impaired the normal development of Biofilm that arrested at the microcolony stage. Alkaline formulations could be promising towards the control of bacterial colonization and therefore the reduction of the Biofilm-related hazard. In the clinical setting, alkaline solutions or cleaners could be promising to prevent the bacterial colonization, by treating surfaces such as catheters or indwelling medical devices, reducing the risk of Biofilm related infections.

Yingkui Yang - One of the best experts on this subject based on the ideXlab platform.

  • Integrated antifouling and bactericidal polymer membranes through bioinspired polydopamine/poly(N-vinyl pyrrolidone) coating
    Applied Surface Science, 2016
    Co-Authors: Xianghong Wang, Shuaishuai Yuan, Yingkui Yang, Tao Jiang, Shifang Luan
    Abstract:

    Abstract Polypropylene (PP) non-woven has been widely used as wound dressing; however, the hydrophobic nature of PP can initiate bacterial attachment and Subsequent Biofilm Formation. Herein, we propose a facile approach to functionalize PP non-woven with poly(ethylene glycol) (PEG) and poly(N-vinyl pyrrolidone)-iodine complex (PVP-I). PVP and PEG were successively tethered onto PP non-woven surface via versatile bioinspired dopamine (DA) chemistry, followed by complexing iodine with PVP moieties. It was demonstrated through the field emission scanning electron microscope (SEM) and spread plate method that the as-modified PP non-woven integrated both antifouling property of PEG for suppressing bacterial adhesion, and bactericidal property of PVP-I for killing the few adherent bacteria. Meanwhile, it could greatly resist platelet and red blood cell adhesion. The integrated antifouling and bactericidal PP non-woven surfaces might have great potential in various wound dressing applications.

  • integrated antifouling and bactericidal polymer membranes through bioinspired polydopamine poly n vinyl pyrrolidone coating
    Applied Surface Science, 2016
    Co-Authors: Shuaishuai Yuan, Xianghong Wang, Yingkui Yang, Tao Jiang, Shifang Luan
    Abstract:

    Abstract Polypropylene (PP) non-woven has been widely used as wound dressing; however, the hydrophobic nature of PP can initiate bacterial attachment and Subsequent Biofilm Formation. Herein, we propose a facile approach to functionalize PP non-woven with poly(ethylene glycol) (PEG) and poly(N-vinyl pyrrolidone)-iodine complex (PVP-I). PVP and PEG were successively tethered onto PP non-woven surface via versatile bioinspired dopamine (DA) chemistry, followed by complexing iodine with PVP moieties. It was demonstrated through the field emission scanning electron microscope (SEM) and spread plate method that the as-modified PP non-woven integrated both antifouling property of PEG for suppressing bacterial adhesion, and bactericidal property of PVP-I for killing the few adherent bacteria. Meanwhile, it could greatly resist platelet and red blood cell adhesion. The integrated antifouling and bactericidal PP non-woven surfaces might have great potential in various wound dressing applications.