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Torleiv Lien - One of the best experts on this subject based on the ideXlab platform.
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desulfobulbus rhabdoformis sp nov a Sulfate Reducer from a water oil separation system
International Journal of Systematic and Evolutionary Microbiology, 1998Co-Authors: Torleiv Lien, Marit Steine Madsen, Ida Helene Steen, Kjersti GjerdevikAbstract:A mesophilic, Gram-negative, rod-shaped, marine, propionate-oxidizing Sulfate Reducer (strain M16T) was isolated from a water-oil separation system on a North Sea oil platform. The optimum conditions for growth were 31 °C, pH 6.8-7.2 and 1.5-2.0 %(w/v) NaCI and 0.1-0.3% (w/v) MgCl26H2O in the medium. The growth yield with Sulfate was 4.6 g cell biomass (mol propionate oxidized)-1. Strain M16Tis nutritionally related to members of the genus Desulfobulbus, but differs in that it has no vitamin requirement and is able to utilize fumarate and malate as carbon and energy sources. Hydrogenase activity measured as hydrogen uptake was mainly membrane-bound and varied with the growth substrate. Highest activity [28 μmol min-1(mg protein)-1] was found in cells grown with hydrogen and lowest [50 nmol min-1(mg protein)-1] in cells grown with propionate as electron donors for Sulfate reduction. Desulforubidin, menaquinone-5(H2) and cytochromes of the c- and b-type were present. The fatty acid pattern was similar to that found for Desulfobulbus propionicus. The DNA base composition was 50.6 mol% G+C. Strain M16Tis equidistantly related to D. propionicus and Desulfobulbus elongatus with 96.1 % 16S rDNA similarity. On the basis of differences in genotypic, phenotypic and immunological characteristics, strain M16T(= DSM 8777T) is proposed as the type strain of a new species, Desulfobulbus rhabdoformis.
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desulfobacter vibrioformis sp nov a Sulfate Reducer from a water oil separation system
International Journal of Systematic and Evolutionary Microbiology, 1997Co-Authors: Torleiv Lien, Janiche BeederAbstract:A mesophilic, gram-negative, vibrio-shaped, marine, acetate-oxidizing Sulfate Reducer (strain B54) was isolated from a water-oil separation system on a North Sea oil platform. The optimum conditions for growth were 33°C, pH 6.8 to 7.0, and concentrations of NaCI and MgCl2 6H2O of at least 1 and 0.3%, respectively. Of various organic acids tested, only acetate was used as an electron and carbon source. The presence of 2-oxoglutarate:dye oxidoreductase suggests acetate oxidation via an operative citric acid cycle. Even though growth of most Desulfobacter strains (including strain B54) did not occur on hydrogen, hydrogenase was detected at low activity. The growth yields were 4.6, 13.1, and 9.6 g of (dry weight) cells per mol of acetate oxidized with Sulfate, sulfite, and thioSulfate, respectively, as electron acceptors. Strain B54 was able to fix dinitrogen. Desulforubidin and cytochromes of the c and b types were present. The G+C content of the DNA was 47 mol%. Strain B54 is most closely related to Desulfobacter latus, with a 16S rDNA sequence similarity of 98.1%. The DNA-DNA relatedness between them was 40.5%. On the basis of differences in genotypic, pheno-typic, and immunological characteristics, we propose that strain B54 is a member of a new species, D. vibrioformis. It can be easily identified and distinguished from other Desulfobacter species by its large, vibrio-shaped cells.
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desulfotomaculum thermocisternum sp nov a Sulfate Reducer isolated from a hot north sea oil reservoir
International Journal of Systematic and Evolutionary Microbiology, 1996Co-Authors: R K Nilsen, Terje Torsvik, Torleiv LienAbstract:The organism described in this paper, strain ST90T (T = type strain), is a thermophilic, spore-forming, rod-shaped Sulfate Reducer that was isolated from North Sea oil reservoir formation water. In cultivation the following substances were used as electron donors and carbon sources: H2-CO2, lactate, pyruvate, ethanol, propanol, butanol, and C3 to C10 and C14 to C17 carboxylic acids. Sulfate was used as the electron acceptor in these reactions. Lactate was incompletely oxidized. Sulfite and thioSulfate were also used as electron acceptors. In the absence of an electron acceptor, the organism grew syntrophically on propionate together with a hydrogenothrophic methanogen. The optimum conditions for growth on lactate and Sulfate were 62°C, pH 6.7, and 50 to 200 mM NaCI. The G+C content was 56 mol%, as determined by high-performance liquid chromatography and 57 mol% as determined by thermal denaturation. Spore formation was observed when the organism was grown on butyrate or propanol as a substrate and at low pH values. On the basis of differences in G+C content and phenotypic and immunological characteristics when the organism was compared with other thermophilic Desulfotomaculum species, we propose that strain ST90T is a member of a new species, Desulfotomaculum thermocisternum. D. thermocisternum can be quickly identified and distinguished from closely related Desulfotomaculum species by immunoblotting.
Jaakko A Puhakka - One of the best experts on this subject based on the ideXlab platform.
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desulfotomaculum alcoholivorax sp nov a moderately thermophilic spore forming Sulfate Reducer isolated from a fluidized bed reactor treating acidic metal and Sulfate containing wastewater
International Journal of Systematic and Evolutionary Microbiology, 2008Co-Authors: Anna H Kaksonen, Stefan Spring, Peter Schumann, Reiner M Kroppenstedt, Jaakko A PuhakkaAbstract:A moderately thermophilic, Gram-positive, endospore-forming, Sulfate-reducing bacterium was isolated from a fluidized-bed reactor treating acidic water containing metal and Sulfate. The strain, designated RE35E1T, was rod-shaped and motile. The temperature range for growth was 33–51 °C (optimum 44–46 °C) and the pH range was 6.0–7.5 (optimum pH 6.4–7.3). The strain grew optimally without additional NaCl. The electron acceptors were 10 mM Sulfate, thioSulfate and elemental sulfur and 1 mM (but not 10 mM) sulfite. Various alcohols and carboxylic acids were utilized as electron donors. Fermentative growth occurred on pyruvate. The cell wall contained meso-diaminopimelic acid, and the major respiratory isoprenoid quinone was menaquinone MK-7. The major whole-cell fatty acids were iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0. Strain RE35E1T was related to representatives of the genera Desulfotomaculum and Sporotomaculum, the closest relatives being Desulfotomaculum arcticum DSM 17038T (96.3 % 16S rRNA gene sequence similarity) and Sporotomaculum hydroxybenzoicum DSM 5475T (92.0 % similarity). Strain RE35E1T represents a novel species, for which the name Desulfotomaculum alcoholivorax sp. nov. is proposed. The type strain is RE35E1T (=DSM 16058T=JCM 14019T).
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desulfurispora thermophila gen nov sp nov a thermophilic spore forming Sulfate Reducer isolated from a sulfidogenic fluidized bed reactor
International Journal of Systematic and Evolutionary Microbiology, 2007Co-Authors: Anna H Kaksonen, Stefan Spring, Peter Schumann, Reiner M Kroppenstedt, Jaakko A PuhakkaAbstract:A thermophilic, Gram-positive, endospore-forming, Sulfate-reducing bacterium was isolated from a sulfidogenic fluidized-bed reactor treating acidic metal- and Sulfate-containing water. The strain, designated RA50E1T, was rod-shaped and motile. The strain grew at 40–67 °C (optimum growth at 59–61 °C) and pH 6.4–7.9 (optimum growth at pH 7.0–7.3). The strain tolerated up to 1 % NaCl. Sulfate, sulfite, thioSulfate and elemental sulfur were used as electron acceptors, but not nitrate, nitrite or iron(III). Electron donors utilized were H2/CO2 (80 : 20, v/v), alcohols, various carboxylic acids and some sugars. Fermentative growth occurred on lactate and pyruvate. The cell wall contained meso-diaminopimelic acid and the major respiratory isoprenoid quinone was menaquinone MK-7. Major whole-cell fatty acids were iso-C15 : 0 and iso-C17 : 0. Strain RA50E1T was distantly related to representatives of the genera Desulfotomaculum, Pelotomaculum, Sporotomaculum and Cryptanaerobacter. On the basis of 16S rRNA gene sequence data, the strain cannot be assigned to any known genus. Based on the phenotypic and phylogenetic features of strain RA50E1T, it is proposed that the strain represents a novel species in a new genus, for which the name Desulfurispora thermophila gen. nov., sp. nov. is proposed. The type strain of Desulfurispora thermophila is RA50E1T (=DSM 16022T=JCM 14018T).
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desulfovirgula thermocuniculi gen nov sp nov a thermophilic Sulfate Reducer isolated from a geothermal underground mine in japan
International Journal of Systematic and Evolutionary Microbiology, 2007Co-Authors: Anna H Kaksonen, Stefan Spring, Peter Schumann, Reiner M Kroppenstedt, Jaakko A PuhakkaAbstract:Thermophilic Sulfate-Reducers have increasingly attractedinterest owing to their potential in biotechnologicalapplications, such as biodesulfurication of flue gases andbiohydrometallurgical processes. The majority of thermo-philic Sulfate-Reducers have been isolated from geothermalhabitats, including oilfield waters (Rosnes et al., 1991;Beeder et al., 1994, 1995), hot springs (Zeikus et al., 1983;Nazina et al., 1989; Karnauchow et al., 1992; Henry et al.,1994) and geothermal groundwaters (Daumas et al., 1988;Love et al., 1993). Thermophilic Sulfate-Reducers includeGram-positivebacteria,suchasDesulfotomaculum(Campbell&Postgate,1965)andThermodesulfobium(Morietal.,2003)within the class ‘Clostridia’. Gram-negative Sulfate-Reducershave been found in genera such as Thermodesulforhabdus(Beeder et al., 1995) and Desulfacinum (Rees et al., 1995)within the class Deltaproteobacteria, Thermodesulfobacterium(Zeikus et al., 1983) and Thermodesulfatator (Moussardet al., 2004) within the class Thermodesulfobacteria andThermodesulfovibrio (Henry et al., 1994) within the class‘Nitrospira’. Additionally, one archaeal Sulfate-reducinggenus,Archaeoglobus (Beederetal.,1994),hasbeendescribed.Recently, we have reported the enrichment and isolation ofthermophilic Sulfate-Reducers from a geothermal under-ground mine in Japan (Kaksonen et al., 2006a, b) and in thispaper we describe a novel thermophilic Sulfate-reducingbacterium, strain RL80JIV
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desulfotomaculum thermosubterraneum sp nov a thermophilic Sulfate Reducer isolated from an underground mine located in a geothermally active area
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Anna H Kaksonen, Stefan Spring, Peter Schumann, Reiner M Kroppenstedt, Jaakko A PuhakkaAbstract:A thermophilic, Gram-positive, endospore-forming, Sulfate-reducing bacterium was isolated from an underground mine in a geothermally active area in Japan. Cells of this strain, designated RL50JIIIT, were rod-shaped and motile. The temperature range for growth was 50–72 °C (optimum growth at 61–66 °C) and the pH range was 6.4–7.8 (optimum at pH 7.2–7.4). Strain RL50JIIIT tolerated up to 1.5 % NaCl, but optimum growth occurred in the presence of 0–1 % NaCl. Electron acceptors utilized were Sulfate, sulfite, thioSulfate and elemental sulfur. Electron donors utilized were H2 in the presence of CO2, alanine, various carboxylic acids and alcohols. Fermentative growth occurred on lactate and pyruvate. The cell wall contained mesodiaminopimelic acid and the major respiratory isoprenoid quinone was menaquinone 7 (MK-7). Major whole-cell fatty acids were iso-C15 : 0, iso-C17 : 0 DMA (dimethyl acetal), iso-C15 : 0 DMA and iso-C17 : 0. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed 98.7 % similarity with Desulfotomaculum solfataricum DSM 14956T. However, DNA–DNA hybridization experiments with Desulfotomaculum kuznetsovii, Desulfotomaculum luciae and D. solfataricum and the G+C content of the DNA (54.4 mol%) allowed the differentiation of strain RL50JIIIT from the recognized species of the genus Desulfotomaculum. Strain RL50JIIIT therefore represents a novel species, for which the name Desulfotomaculum thermosubterraneum sp. nov. is proposed. The type strain is RL50JIIIT (=DSM 16057T=JCM 13837T).
Cristiana Cravolaureau - One of the best experts on this subject based on the ideXlab platform.
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correction for grossi et al mono and dialkyl glycerol ether lipids in anaerobic bacteria biosynthetic insights from the mesophilic Sulfate Reducer desulfatibacillum alkenivorans pf2803t
Applied and Environmental Microbiology, 2015Co-Authors: Vincent Grossi, Damien Mollex, Arnauld Vinconlaugier, Florence Hakil, Muriel Pacton, Cristiana CravolaureauAbstract:Volume 81, no. 9, p. 3157–3168, 2015. Page 3162, Table 1: The labeled fatty acid corresponding to the 1,1,2,2-D4-hexadecanol substrate (row 3, column 2) should appear as shown below (i.e., without the brackets and asterisks that previously suggested it was a growth substrate). ![Figure][1]
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mono and dialkyl glycerol ether lipids in anaerobic bacteria biosynthetic insights from the mesophilic Sulfate Reducer desulfatibacillum alkenivorans pf2803t
Applied and Environmental Microbiology, 2015Co-Authors: Vincent Grossi, Damien Mollex, Arnauld Vinconlaugier, Florence Hakil, Muriel Pacton, Cristiana CravolaureauAbstract:Bacterial glycerol ether lipids (alkylglycerols) have received increasing attention during the last decades, notably due to their potential role in cell resistance or adaptation to adverse environmental conditions. Major uncertainties remain, however, regarding the origin, biosynthesis, and modes of formation of these uncommon bacterial lipids. We report here the preponderance of monoalkyl- and dialkylglycerols (1-O-alkyl-, 2-O-alkyl-, and 1,2-O-dialkylglycerols) among the hydrolyzed lipids of the marine mesophilic Sulfate-reducing proteobacterium Desulfatibacillum alkenivorans PF2803T grown on n-alkenes (pentadec-1-ene or hexadec-1-ene) as the sole carbon and energy source. Alkylglycerols account for one-third to two-thirds of the total cellular lipids (alkylglycerols plus acylglycerols), depending on the growth substrate, with dialkylglycerols contributing to one-fifth to two-fifths of the total ether lipids. The carbon chain distribution of the lipids of D. alkenivorans also depends on that of the substrate, but the chain length and methyl-branching patterns of fatty acids and monoalkyl- and dialkylglycerols are systematically congruent, supporting the idea of a biosynthetic link between the three classes of compounds. Vinyl ethers (1-alken-1′-yl-glycerols, known as plasmalogens) are not detected among the lipids of strain PF2803T. Cultures grown on different (per)deuterated n-alkene, n-alkanol, and n-fatty acid substrates further demonstrate that saturated alkylglycerols are not formed via the reduction of hypothetic alken-1′-yl intermediates. Our results support an unprecedented biosynthetic pathway to monoalkyl/monoacyl- and dialkylglycerols in anaerobic bacteria and suggest that n-alkyl compounds present in the environment can serve as the substrates for supplying the building blocks of ether phospholipids of heterotrophic bacteria.
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anaerobic 1 alkene metabolism by the alkane and alkene degrading Sulfate Reducer desulfatibacillum aliphaticivorans strain cv2803t
Applied and Environmental Microbiology, 2007Co-Authors: Vincent Grossi, Cristiana Cravolaureau, Alain Meou, Danielle Raphel, Frederic Garzino, Agnes HirschlerreaAbstract:The alkane- and alkene-degrading, marine Sulfate-reducing bacterium Desulfatibacillum aliphaticivorans strain CV2803T, known to oxidize n-alkanes anaerobically by fumarate addition at C-2, was investigated for its 1-alkene metabolism. The total cellular fatty acids of this strain were predominantly C-(even number) (C-even) when it was grown on C-even 1-alkenes and predominantly C-(odd number) (C-odd) when it was grown on C-odd 1-alkenes. Detailed analyses of those fatty acids by gas chromatography-mass spectrometry after 6- to 10-week incubations allowed the identification of saturated 2- and 4-ethyl-, 2- and 4-methyl-, and monounsaturated 4-methyl-branched fatty acids with chain lengths that correlated with those of the 1-alkene. The growth of D. aliphaticivorans on (per)deuterated 1-alkenes provided direct evidence of the anaerobic transformation of these alkenes into the corresponding 1-alcohols and into linear as well as 10- and 4-methyl-branched fatty acids. Experiments performed with [13C]bicarbonate indicated that the initial activation of 1-alkene by the addition of inorganic carbon does not occur. These results demonstrate that D. aliphaticivorans metabolizes 1-alkene by the oxidation of the double bond at C-1 and by the subterminal addition of organic carbon at both ends of the molecule [C-2 and C-(ω-1)]. The detection of ethyl-branched fatty acids from unlabeled 1-alkenes further suggests that carbon addition also occurs at C-3. Alkylsuccinates were not observed as potential initial intermediates in alkene metabolism. Based on our observations, the first pathways for anaerobic 1-alkene metabolism in an anaerobic bacterium are proposed. Those pathways indicate that diverse initial reactions of 1-alkene activation can occur simultaneously in the same strain of Sulfate-reducing bacterium.
Vincent Grossi - One of the best experts on this subject based on the ideXlab platform.
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correction for grossi et al mono and dialkyl glycerol ether lipids in anaerobic bacteria biosynthetic insights from the mesophilic Sulfate Reducer desulfatibacillum alkenivorans pf2803t
Applied and Environmental Microbiology, 2015Co-Authors: Vincent Grossi, Damien Mollex, Arnauld Vinconlaugier, Florence Hakil, Muriel Pacton, Cristiana CravolaureauAbstract:Volume 81, no. 9, p. 3157–3168, 2015. Page 3162, Table 1: The labeled fatty acid corresponding to the 1,1,2,2-D4-hexadecanol substrate (row 3, column 2) should appear as shown below (i.e., without the brackets and asterisks that previously suggested it was a growth substrate). ![Figure][1]
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mono and dialkyl glycerol ether lipids in anaerobic bacteria biosynthetic insights from the mesophilic Sulfate Reducer desulfatibacillum alkenivorans pf2803t
Applied and Environmental Microbiology, 2015Co-Authors: Vincent Grossi, Damien Mollex, Arnauld Vinconlaugier, Florence Hakil, Muriel Pacton, Cristiana CravolaureauAbstract:Bacterial glycerol ether lipids (alkylglycerols) have received increasing attention during the last decades, notably due to their potential role in cell resistance or adaptation to adverse environmental conditions. Major uncertainties remain, however, regarding the origin, biosynthesis, and modes of formation of these uncommon bacterial lipids. We report here the preponderance of monoalkyl- and dialkylglycerols (1-O-alkyl-, 2-O-alkyl-, and 1,2-O-dialkylglycerols) among the hydrolyzed lipids of the marine mesophilic Sulfate-reducing proteobacterium Desulfatibacillum alkenivorans PF2803T grown on n-alkenes (pentadec-1-ene or hexadec-1-ene) as the sole carbon and energy source. Alkylglycerols account for one-third to two-thirds of the total cellular lipids (alkylglycerols plus acylglycerols), depending on the growth substrate, with dialkylglycerols contributing to one-fifth to two-fifths of the total ether lipids. The carbon chain distribution of the lipids of D. alkenivorans also depends on that of the substrate, but the chain length and methyl-branching patterns of fatty acids and monoalkyl- and dialkylglycerols are systematically congruent, supporting the idea of a biosynthetic link between the three classes of compounds. Vinyl ethers (1-alken-1′-yl-glycerols, known as plasmalogens) are not detected among the lipids of strain PF2803T. Cultures grown on different (per)deuterated n-alkene, n-alkanol, and n-fatty acid substrates further demonstrate that saturated alkylglycerols are not formed via the reduction of hypothetic alken-1′-yl intermediates. Our results support an unprecedented biosynthetic pathway to monoalkyl/monoacyl- and dialkylglycerols in anaerobic bacteria and suggest that n-alkyl compounds present in the environment can serve as the substrates for supplying the building blocks of ether phospholipids of heterotrophic bacteria.
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anaerobic 1 alkene metabolism by the alkane and alkene degrading Sulfate Reducer desulfatibacillum aliphaticivorans strain cv2803t
Applied and Environmental Microbiology, 2007Co-Authors: Vincent Grossi, Cristiana Cravolaureau, Alain Meou, Danielle Raphel, Frederic Garzino, Agnes HirschlerreaAbstract:The alkane- and alkene-degrading, marine Sulfate-reducing bacterium Desulfatibacillum aliphaticivorans strain CV2803T, known to oxidize n-alkanes anaerobically by fumarate addition at C-2, was investigated for its 1-alkene metabolism. The total cellular fatty acids of this strain were predominantly C-(even number) (C-even) when it was grown on C-even 1-alkenes and predominantly C-(odd number) (C-odd) when it was grown on C-odd 1-alkenes. Detailed analyses of those fatty acids by gas chromatography-mass spectrometry after 6- to 10-week incubations allowed the identification of saturated 2- and 4-ethyl-, 2- and 4-methyl-, and monounsaturated 4-methyl-branched fatty acids with chain lengths that correlated with those of the 1-alkene. The growth of D. aliphaticivorans on (per)deuterated 1-alkenes provided direct evidence of the anaerobic transformation of these alkenes into the corresponding 1-alcohols and into linear as well as 10- and 4-methyl-branched fatty acids. Experiments performed with [13C]bicarbonate indicated that the initial activation of 1-alkene by the addition of inorganic carbon does not occur. These results demonstrate that D. aliphaticivorans metabolizes 1-alkene by the oxidation of the double bond at C-1 and by the subterminal addition of organic carbon at both ends of the molecule [C-2 and C-(ω-1)]. The detection of ethyl-branched fatty acids from unlabeled 1-alkenes further suggests that carbon addition also occurs at C-3. Alkylsuccinates were not observed as potential initial intermediates in alkene metabolism. Based on our observations, the first pathways for anaerobic 1-alkene metabolism in an anaerobic bacterium are proposed. Those pathways indicate that diverse initial reactions of 1-alkene activation can occur simultaneously in the same strain of Sulfate-reducing bacterium.
Anna H Kaksonen - One of the best experts on this subject based on the ideXlab platform.
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desulfotomaculum alcoholivorax sp nov a moderately thermophilic spore forming Sulfate Reducer isolated from a fluidized bed reactor treating acidic metal and Sulfate containing wastewater
International Journal of Systematic and Evolutionary Microbiology, 2008Co-Authors: Anna H Kaksonen, Stefan Spring, Peter Schumann, Reiner M Kroppenstedt, Jaakko A PuhakkaAbstract:A moderately thermophilic, Gram-positive, endospore-forming, Sulfate-reducing bacterium was isolated from a fluidized-bed reactor treating acidic water containing metal and Sulfate. The strain, designated RE35E1T, was rod-shaped and motile. The temperature range for growth was 33–51 °C (optimum 44–46 °C) and the pH range was 6.0–7.5 (optimum pH 6.4–7.3). The strain grew optimally without additional NaCl. The electron acceptors were 10 mM Sulfate, thioSulfate and elemental sulfur and 1 mM (but not 10 mM) sulfite. Various alcohols and carboxylic acids were utilized as electron donors. Fermentative growth occurred on pyruvate. The cell wall contained meso-diaminopimelic acid, and the major respiratory isoprenoid quinone was menaquinone MK-7. The major whole-cell fatty acids were iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0. Strain RE35E1T was related to representatives of the genera Desulfotomaculum and Sporotomaculum, the closest relatives being Desulfotomaculum arcticum DSM 17038T (96.3 % 16S rRNA gene sequence similarity) and Sporotomaculum hydroxybenzoicum DSM 5475T (92.0 % similarity). Strain RE35E1T represents a novel species, for which the name Desulfotomaculum alcoholivorax sp. nov. is proposed. The type strain is RE35E1T (=DSM 16058T=JCM 14019T).
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desulfurispora thermophila gen nov sp nov a thermophilic spore forming Sulfate Reducer isolated from a sulfidogenic fluidized bed reactor
International Journal of Systematic and Evolutionary Microbiology, 2007Co-Authors: Anna H Kaksonen, Stefan Spring, Peter Schumann, Reiner M Kroppenstedt, Jaakko A PuhakkaAbstract:A thermophilic, Gram-positive, endospore-forming, Sulfate-reducing bacterium was isolated from a sulfidogenic fluidized-bed reactor treating acidic metal- and Sulfate-containing water. The strain, designated RA50E1T, was rod-shaped and motile. The strain grew at 40–67 °C (optimum growth at 59–61 °C) and pH 6.4–7.9 (optimum growth at pH 7.0–7.3). The strain tolerated up to 1 % NaCl. Sulfate, sulfite, thioSulfate and elemental sulfur were used as electron acceptors, but not nitrate, nitrite or iron(III). Electron donors utilized were H2/CO2 (80 : 20, v/v), alcohols, various carboxylic acids and some sugars. Fermentative growth occurred on lactate and pyruvate. The cell wall contained meso-diaminopimelic acid and the major respiratory isoprenoid quinone was menaquinone MK-7. Major whole-cell fatty acids were iso-C15 : 0 and iso-C17 : 0. Strain RA50E1T was distantly related to representatives of the genera Desulfotomaculum, Pelotomaculum, Sporotomaculum and Cryptanaerobacter. On the basis of 16S rRNA gene sequence data, the strain cannot be assigned to any known genus. Based on the phenotypic and phylogenetic features of strain RA50E1T, it is proposed that the strain represents a novel species in a new genus, for which the name Desulfurispora thermophila gen. nov., sp. nov. is proposed. The type strain of Desulfurispora thermophila is RA50E1T (=DSM 16022T=JCM 14018T).
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desulfovirgula thermocuniculi gen nov sp nov a thermophilic Sulfate Reducer isolated from a geothermal underground mine in japan
International Journal of Systematic and Evolutionary Microbiology, 2007Co-Authors: Anna H Kaksonen, Stefan Spring, Peter Schumann, Reiner M Kroppenstedt, Jaakko A PuhakkaAbstract:Thermophilic Sulfate-Reducers have increasingly attractedinterest owing to their potential in biotechnologicalapplications, such as biodesulfurication of flue gases andbiohydrometallurgical processes. The majority of thermo-philic Sulfate-Reducers have been isolated from geothermalhabitats, including oilfield waters (Rosnes et al., 1991;Beeder et al., 1994, 1995), hot springs (Zeikus et al., 1983;Nazina et al., 1989; Karnauchow et al., 1992; Henry et al.,1994) and geothermal groundwaters (Daumas et al., 1988;Love et al., 1993). Thermophilic Sulfate-Reducers includeGram-positivebacteria,suchasDesulfotomaculum(Campbell&Postgate,1965)andThermodesulfobium(Morietal.,2003)within the class ‘Clostridia’. Gram-negative Sulfate-Reducershave been found in genera such as Thermodesulforhabdus(Beeder et al., 1995) and Desulfacinum (Rees et al., 1995)within the class Deltaproteobacteria, Thermodesulfobacterium(Zeikus et al., 1983) and Thermodesulfatator (Moussardet al., 2004) within the class Thermodesulfobacteria andThermodesulfovibrio (Henry et al., 1994) within the class‘Nitrospira’. Additionally, one archaeal Sulfate-reducinggenus,Archaeoglobus (Beederetal.,1994),hasbeendescribed.Recently, we have reported the enrichment and isolation ofthermophilic Sulfate-Reducers from a geothermal under-ground mine in Japan (Kaksonen et al., 2006a, b) and in thispaper we describe a novel thermophilic Sulfate-reducingbacterium, strain RL80JIV
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desulfotomaculum thermosubterraneum sp nov a thermophilic Sulfate Reducer isolated from an underground mine located in a geothermally active area
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Anna H Kaksonen, Stefan Spring, Peter Schumann, Reiner M Kroppenstedt, Jaakko A PuhakkaAbstract:A thermophilic, Gram-positive, endospore-forming, Sulfate-reducing bacterium was isolated from an underground mine in a geothermally active area in Japan. Cells of this strain, designated RL50JIIIT, were rod-shaped and motile. The temperature range for growth was 50–72 °C (optimum growth at 61–66 °C) and the pH range was 6.4–7.8 (optimum at pH 7.2–7.4). Strain RL50JIIIT tolerated up to 1.5 % NaCl, but optimum growth occurred in the presence of 0–1 % NaCl. Electron acceptors utilized were Sulfate, sulfite, thioSulfate and elemental sulfur. Electron donors utilized were H2 in the presence of CO2, alanine, various carboxylic acids and alcohols. Fermentative growth occurred on lactate and pyruvate. The cell wall contained mesodiaminopimelic acid and the major respiratory isoprenoid quinone was menaquinone 7 (MK-7). Major whole-cell fatty acids were iso-C15 : 0, iso-C17 : 0 DMA (dimethyl acetal), iso-C15 : 0 DMA and iso-C17 : 0. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed 98.7 % similarity with Desulfotomaculum solfataricum DSM 14956T. However, DNA–DNA hybridization experiments with Desulfotomaculum kuznetsovii, Desulfotomaculum luciae and D. solfataricum and the G+C content of the DNA (54.4 mol%) allowed the differentiation of strain RL50JIIIT from the recognized species of the genus Desulfotomaculum. Strain RL50JIIIT therefore represents a novel species, for which the name Desulfotomaculum thermosubterraneum sp. nov. is proposed. The type strain is RL50JIIIT (=DSM 16057T=JCM 13837T).
