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I. A. Tsaplina - One of the best experts on this subject based on the ideXlab platform.
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Sulfobacillus thermotolerans: new insights into resistance and metabolic capacities of acidophilic chemolithotrophs.
Scientific reports, 2019Co-Authors: A. E. Panyushkina, I. A. Tsaplina, Vladislav V. Babenko, Anastasia S. Nikitina, Oksana V. Selezneva, Maria A. Letarova, Elena S. Kostryukova, Andrey V. LetarovAbstract:The first complete genome of the biotechnologically important species Sulfobacillus thermotolerans has been sequenced. Its 3 317 203-bp chromosome contains an 83 269-bp plasmid-like region, which carries heavy metal resistance determinants and the rusticyanin gene. Plasmid-mediated metal resistance is unusual for acidophilic chemolithotrophs. Moreover, most of their plasmids are cryptic and do not contribute to the phenotype of the host cells. A polyphosphate-based mechanism of metal resistance, which has been previously unknown in the genus Sulfobacillus or other Gram-positive chemolithotrophs, potentially operates in two Sulfobacillus species. The methylcitrate cycle typical for pathogens and identified in the genus Sulfobacillus for the first time can fulfill the energy and/or protective function in S. thermotolerans Kr1 and two other Sulfobacillus species, which have incomplete glyoxylate cycles. It is notable that the TCA cycle, disrupted in all Sulfobacillus isolates under optimal growth conditions, proved to be complete in the cells enduring temperature stress. An efficient antioxidant defense system gives S. thermotolerans another competitive advantage in the microbial communities inhabiting acidic metal-rich environments. The genomic comparisons revealed 80 unique genes in the strain Kr1, including those involved in lactose/galactose catabolism. The results provide new insights into metabolism and resistance mechanisms in the Sulfobacillus genus and other acidophiles.
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Biochemical Aspects of Energy Metabolism in Sulfobacillus thermotolerans
Solid State Phenomena, 2017Co-Authors: A. E. Panyushkina, V. S. Melamud, I. A. TsaplinaAbstract:Bacteria of the genus Sulfobacillus are successfully used in biotechnologies of treatment of sulfide ore materials. However, energy processes are still poorly understood in sulfobacilli. Several enzymes encoded in the genomes of sulfobacilli are putatively involved in the initial phase of Fe2+ oxidation. Cytochromes c, sulfocyanins, and rusticyanin-like proteins were revealed in genomes of these bacteria. Thus, a membrane bound cytochrome c can be reduced directly by Fe2+, and electrons can be further transferred to sulfocyanin, rusticyanin or some other components of the transport chain. HPLC identification of prosthetic groups of cytochromes predicts the possible presence of heme c in the cells of thermotolerant Sulfobacillus. Determination of metal content in the cells shows that Sulfobacillus harbours higher levels of copper ions under specific conditions. This can be explained by higher expression of copper-containing proteins, including cupredoxins.
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Optimization of bioleaching and oxidation of gold-bearing pyrite-arsnopyrite ore concentrate in batch mode
Mikrobiologiia, 2014Co-Authors: N V Grigor'eva, I. A. Tsaplina, A E Paniushkina, Tamara F. Kondrat'evaAbstract:Biooxidation of refractory gold-bearing pyrite-arsenopyrite flotation concentrate was optimized and aburidance of predominant groups in the community ofthermophilic acidophilic chemolithotrophic microorganisms at various stages ofbioleaching was determined. The optimal parameters for growth and leaching/oxidation of the mineral components of the concentrate were pSH 1.4, 1.6-1.8; 47.5 degrees C; and the following salt concentrations in the liquid phase (g/L): K2HPO4.3H2O, 0.53; (NH4)2SO4, 1.6 and MgSO4.7H2O, 2.5 (or (NH4)2SO4, 1.23; ammophos, 0.41; KOH, 0.1) with 0.03% yeast extract. The optimal conditions resulted in high growth rate, high levels of iron andarsenic leaching, of Fe2+ and S(2-)/S0 oxidation, and predominance of Acidithiobacillus caldus, Sulfobacillus spp., and Ferroplasma spp. in the community.
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Selection of a community of acidochemolithotrophic microorganisms with a high oxidation rate of pyrrhotite- containing sulphide ore flotatation concentrate
Prikladnaia biokhimiia i mikrobiologiia, 2013Co-Authors: Tamara F. Kondrat'eva, T. A. Pivovarova, A. G. Bulaev, V. S. Melamud, P. V. Moshchanetskii, I. A. Tsaplina, N. V. Grigor’eva, A. E. Zhuravleva, A. V. BelyiAbstract:A community of acidochemolithotrophic microorganisms with a high oxidation rate of pyrrhotite-containing sulphide ore flotation concentrate was selected. The Acidithiobacillus caldus OP-1 and Ferroplasma acidiphilum OP-2 cultures were identified to be dominating members. The presence of the Acidithiobacillus ferrooxidans OP-3, Leptospirillum ferriphilum OP-4, and Sulfobacillus thermosulfidooxidans OP-5 cultures in the community’s composition was also mentioned. The analysis results of solid residues of the process showed a greater elemental sulfur oxidation level and gold recovery when the initial pH value in tank I was maintained at a level of 1.8–2.0 (90.5%) rather than 1.6–1.8 (86.3%).
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Strain polymorphism of the plasmid profiles in Sulfobacillus species
Microbiology, 2009Co-Authors: O. V. Tupikina, T. A. Pivovarova, V. S. Melamud, I. A. Tsaplina, A. E. Zhuravleva, T. I. Bogdanova, Tamara F. Kondrat'evaAbstract:Plasmids were discovered for the first time in strains belonging to different species of the genus Sulfobacillus: S. thermosulfidooxidans, S. sibiricus, S. thermotolerans, “S. olympiadicus”, and S. acidophilus. The plasmids were detected in the cells of four out of eight strains grown on a medium with ferrous iron. Adaptation to elementary sulfur was accompanied by changes in the plasmid profiles in two out of seven strains. Plasmids were detected in all the studied strains of sulfobacilli after adaptation to the pyrite-arsenopyrite ore concentrate from the Nezhdaninskoe deposit containing gold, silver, zinc, copper, and lead. No plasmids were found in S. thermotolerans Kr1T after four transfers on a medium containing iron and 0.018 mM Ag+. After adaptation of the same strain to 765 mM Zn2+, only one plasmid was found in the cells, the largest among those detected earlier in this culture adapted to the Nezhdaninskoe ore concentrate. The strain S. thermotolerans Kr1T, after four transfers on media with either 78 mM Cu2+ or 2 mM Pb2+, did not contain plasmids. The presence of plasmids in the cells of sulfobacilli did not influence their resistance to the ions of the studied metals.
Tamara F. Kondrat'eva - One of the best experts on this subject based on the ideXlab platform.
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Optimization of bioleaching and oxidation of gold-bearing pyrite-arsnopyrite ore concentrate in batch mode
Mikrobiologiia, 2014Co-Authors: N V Grigor'eva, I. A. Tsaplina, A E Paniushkina, Tamara F. Kondrat'evaAbstract:Biooxidation of refractory gold-bearing pyrite-arsenopyrite flotation concentrate was optimized and aburidance of predominant groups in the community ofthermophilic acidophilic chemolithotrophic microorganisms at various stages ofbioleaching was determined. The optimal parameters for growth and leaching/oxidation of the mineral components of the concentrate were pSH 1.4, 1.6-1.8; 47.5 degrees C; and the following salt concentrations in the liquid phase (g/L): K2HPO4.3H2O, 0.53; (NH4)2SO4, 1.6 and MgSO4.7H2O, 2.5 (or (NH4)2SO4, 1.23; ammophos, 0.41; KOH, 0.1) with 0.03% yeast extract. The optimal conditions resulted in high growth rate, high levels of iron andarsenic leaching, of Fe2+ and S(2-)/S0 oxidation, and predominance of Acidithiobacillus caldus, Sulfobacillus spp., and Ferroplasma spp. in the community.
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Selection of a community of acidochemolithotrophic microorganisms with a high oxidation rate of pyrrhotite- containing sulphide ore flotatation concentrate
Prikladnaia biokhimiia i mikrobiologiia, 2013Co-Authors: Tamara F. Kondrat'eva, T. A. Pivovarova, A. G. Bulaev, V. S. Melamud, P. V. Moshchanetskii, I. A. Tsaplina, N. V. Grigor’eva, A. E. Zhuravleva, A. V. BelyiAbstract:A community of acidochemolithotrophic microorganisms with a high oxidation rate of pyrrhotite-containing sulphide ore flotation concentrate was selected. The Acidithiobacillus caldus OP-1 and Ferroplasma acidiphilum OP-2 cultures were identified to be dominating members. The presence of the Acidithiobacillus ferrooxidans OP-3, Leptospirillum ferriphilum OP-4, and Sulfobacillus thermosulfidooxidans OP-5 cultures in the community’s composition was also mentioned. The analysis results of solid residues of the process showed a greater elemental sulfur oxidation level and gold recovery when the initial pH value in tank I was maintained at a level of 1.8–2.0 (90.5%) rather than 1.6–1.8 (86.3%).
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Strain polymorphism of the plasmid profiles in Sulfobacillus species
Microbiology, 2009Co-Authors: O. V. Tupikina, T. A. Pivovarova, V. S. Melamud, I. A. Tsaplina, A. E. Zhuravleva, T. I. Bogdanova, Tamara F. Kondrat'evaAbstract:Plasmids were discovered for the first time in strains belonging to different species of the genus Sulfobacillus: S. thermosulfidooxidans, S. sibiricus, S. thermotolerans, “S. olympiadicus”, and S. acidophilus. The plasmids were detected in the cells of four out of eight strains grown on a medium with ferrous iron. Adaptation to elementary sulfur was accompanied by changes in the plasmid profiles in two out of seven strains. Plasmids were detected in all the studied strains of sulfobacilli after adaptation to the pyrite-arsenopyrite ore concentrate from the Nezhdaninskoe deposit containing gold, silver, zinc, copper, and lead. No plasmids were found in S. thermotolerans Kr1T after four transfers on a medium containing iron and 0.018 mM Ag+. After adaptation of the same strain to 765 mM Zn2+, only one plasmid was found in the cells, the largest among those detected earlier in this culture adapted to the Nezhdaninskoe ore concentrate. The strain S. thermotolerans Kr1T, after four transfers on media with either 78 mM Cu2+ or 2 mM Pb2+, did not contain plasmids. The presence of plasmids in the cells of sulfobacilli did not influence their resistance to the ions of the studied metals.
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Phenotypic properties of Sulfobacillus thermotolerans: comparative aspects
Microbiology, 2008Co-Authors: I. A. Tsaplina, A. E. Zhuravleva, E. N. Krasil’nikova, L. M. Zakharchuk, M. A. Egorova, T. I. Bogdanova, N. E. Suzina, V. I. Duda, I. N. Stadnichuk, Tamara F. Kondrat'evaAbstract:The phenotypic characteristics of the species Sulfobacillus thermotolerans Kr1(T), as dependent on the cultivation conditions, are described in detail. High growth rates (0.22-0.30 h(-1)) and high oxidative activity were recorded under optimum mixotrophic conditions at 40 degrees C on medium with inorganic (Fe(II), S(0), or pyrite-arsenopyrite concentrate) and organic (glucose and/or yeast extract) substrates. In cells grown under optimum conditions on medium with iron, hemes a, b, and, most probably, c were present, indicating the presence of the corresponding cytochromes. Peculiar extended structures in the form of cylindrical cords, never observed previously, were revealed; a mucous matrix, likely of polysaccharide nature, occurred around the cells. In the cells of sulfobacilli grown litho-, organo-, and mixotrophically at 40 degrees C, the enzymes of the three main pathways of carbon utilization and some enzymes of the TCA cycle were revealed. The enzyme activity was maximum under mixotrophic growth conditions. The growth rate in the regions of limiting temperatures (55 degrees C and 12-14 degrees C) decreased two- and tenfold, respectively; no activity of 6-phosphogluconate dehydrogenase, one of the key enzymes of the oxidative pentose phosphate pathway, could be revealed; and a decrease in the activity of almost all enzymes of glucose metabolism and of the TCA cycle was observed. The rate of 14CO2 fixation by cells under auto-, mixo-, and heterotrophic conditions constituted 31.8, 23.3, and 10.3 nmol/(h mg protein), respectively. The activities of RuBP carboxylase (it peaked during lithotrophic growth) and of carboxylases of heterotrophic carbon dioxide fixation were recorded. The physiological and biochemical peculiarities of the thermotolerant Sulfobacillus are compared versus moderately thermophilic sulfobacilli.
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Sulfobacillus thermotolerans sp. nov., a thermotolerant, chemolithotrophic bacterium.
International journal of systematic and evolutionary microbiology, 2006Co-Authors: Tat'yana I Bogdanova, Tamara F. Kondrat'eva, V. S. Melamud, I. A. Tsaplina, Vitalii I Duda, Natalya E Suzina, Tat'yana P Tourova, Grigorii I KaravaikoAbstract:A thermotolerant, Gram-positive, aerobic, endospore-forming, acidophilic bacterium (strain Kr1T) was isolated from the pulp of a gold-containing sulfide concentrate processed at 40 degrees C in a gold-recovery plant (Siberia). Cells of strain Kr1(T) were straight to slightly curved rods, 0.8-1.2 microm in diameter and 1.5-4.5 microm in length. Strain Kr1T formed spherical and oval, refractile, subterminally located endospores. The temperature range for growth was 20-60 degrees C, with an optimum at 40 degrees C. The pH range for growth on medium containing ferrous iron was 1.2-2.4, with an optimum at pH 2.0; the pH range for growth on medium containing S0 was 2.0-5.0, with an optimum at pH 2.5. Strain Kr1T was mixotrophic, oxidizing ferrous iron, S0, tetrathionate or sulfide minerals as energy sources in the presence of 0.02 % yeast extract or other organic substrates. The G+C content of the DNA of strain Kr1T was 48.2+/-0.5 mol%. Strain Kr1T showed a low level of DNA-DNA reassociation with the known Sulfobacillus species (11-44 %). 16S rRNA gene sequence analysis revealed that Kr1T formed a separate phylogenetic group with a high degree of similarity between the nucleotide sequences (98.3-99.6 %) and 100 % bootstrap support within the phylogenetic Sulfobacillus cluster. On the basis of its physiological properties and the results of phylogenetic analyses, strain Kr1T can be affiliated to a novel species of the genus Sulfobacillus, for which the name Sulfobacillus thermotolerans sp. nov. is proposed. The type strain is Kr1T (=VKM B-2339T=DSM 17362T).
Tat'yana P Tourova - One of the best experts on this subject based on the ideXlab platform.
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Sulfobacillus thermotolerans sp. nov., a thermotolerant, chemolithotrophic bacterium.
International journal of systematic and evolutionary microbiology, 2006Co-Authors: Tat'yana I Bogdanova, Tamara F. Kondrat'eva, V. S. Melamud, I. A. Tsaplina, Vitalii I Duda, Natalya E Suzina, Tat'yana P Tourova, Grigorii I KaravaikoAbstract:A thermotolerant, Gram-positive, aerobic, endospore-forming, acidophilic bacterium (strain Kr1T) was isolated from the pulp of a gold-containing sulfide concentrate processed at 40 degrees C in a gold-recovery plant (Siberia). Cells of strain Kr1(T) were straight to slightly curved rods, 0.8-1.2 microm in diameter and 1.5-4.5 microm in length. Strain Kr1T formed spherical and oval, refractile, subterminally located endospores. The temperature range for growth was 20-60 degrees C, with an optimum at 40 degrees C. The pH range for growth on medium containing ferrous iron was 1.2-2.4, with an optimum at pH 2.0; the pH range for growth on medium containing S0 was 2.0-5.0, with an optimum at pH 2.5. Strain Kr1T was mixotrophic, oxidizing ferrous iron, S0, tetrathionate or sulfide minerals as energy sources in the presence of 0.02 % yeast extract or other organic substrates. The G+C content of the DNA of strain Kr1T was 48.2+/-0.5 mol%. Strain Kr1T showed a low level of DNA-DNA reassociation with the known Sulfobacillus species (11-44 %). 16S rRNA gene sequence analysis revealed that Kr1T formed a separate phylogenetic group with a high degree of similarity between the nucleotide sequences (98.3-99.6 %) and 100 % bootstrap support within the phylogenetic Sulfobacillus cluster. On the basis of its physiological properties and the results of phylogenetic analyses, strain Kr1T can be affiliated to a novel species of the genus Sulfobacillus, for which the name Sulfobacillus thermotolerans sp. nov. is proposed. The type strain is Kr1T (=VKM B-2339T=DSM 17362T).
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Reclassification of 'Sulfobacillus thermosulfidooxidans subsp. thermotolerans' strain K1 as Alicyclobacillus tolerans sp. nov. and Sulfobacillus disulfidooxidans Dufresne et al. 1996 as Alicyclobacillus disulfidooxidans comb. nov., and emended descri
International Journal of Systematic and Evolutionary Microbiology, 2005Co-Authors: G. I. Karavaiko, Tamara F. Kondrat'eva, I. A. Tsaplina, Tat'yana P Tourova, E. N. Krasil’nikova, T. I. Bogdanova, Marya A Egorova, Leonid M ZakharchukAbstract:Comparative analysis of 16S rRNA gene sequences, DNA–DNA hybridization data and phenotypic properties revealed that ‘Sulfobacillus thermosulfidooxidans subsp. thermotolerans’ strain K1 is not a member of the genus Sulfobacillus. Phylogenetically, strain K1 is closely related to unclassified strains of the genus Alicyclobacillus: the 16S rRNA gene sequence of strain K1 is similar to that of Alicyclobacillus sp. AGC-2 (99·6 %), Alicyclobacillus sp. 5C (98·9 %) and Alicyclobacillus sp. CLG (98·6 %) and bacterium GSM (99·1 %). The 16S rRNA gene sequence similarity values for strain K1 and species of the genus Alicyclobacillus with validly published names were in the range 92·1–94·6 %, and for S. thermosulfidooxidans VKM B-1269T the value was 87·7 %. Sulfobacillus disulfidooxidans SD-11T was also phylogenetically related to strain K1 (92·6 % sequence similarity) and thus belonged to the genus Alicyclobacillus. Chemotaxonomic data, such as the major cell-membrane lipid components of strains K1 and SD-11T (ω-alicyclic fatty acids) and the major isoprenoid quinone (menaquinone MK-7) of strain K1, supported the affiliation of strains K1 and SD-11T to the genus Alicyclobacillus. Physiological and molecular biological tests allowed genotypic and phenotypic differentiation of strains K1 and SD-11T from the nine Alicyclobacillus species with validly published names. The G+C content of the DNA of strain K1 was 48·7±0·6 mol%; that of strain SD-11T was 53±1 mol%. DNA–DNA reassociation studies showed low relatedness (22 %) between strains K1 and SD-11T, and even lower relatedness (3–5 %) between these strains and Alicyclobacillus acidocaldarius subsp. acidocaldarius ATCC 27009T, DSM 446T. DNA reassociation of strains K1 and SD-11T with Alicyclobacillus cycloheptanicus DSM 4006T gave values of 15 and 21, respectively. Based on the phenotypic and phylogenetic characteristics of strains K1 and SD-11T, Alicyclobacillus tolerans sp. nov. (type strain, K1T=VKM B-2304T=DSM 16297T) and Alicyclobacillus disulfidooxidans comb. nov. (type strain, SD-11T=ATCC 51911T=DSM 12064T) are proposed.
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Reclassification of 'Sulfobacillus thermosulfidooxidans subsp. thermotolerans' strain K1 as Alicyclobacillus tolerans sp. nov. and Sulfobacillus disulfidooxidans Dufresne et al. 1996 as Alicyclobacillus disulfidooxidans comb. nov., and emended descri
International journal of systematic and evolutionary microbiology, 2005Co-Authors: Grigorii I Karavaiko, Tamara F. Kondrat'eva, I. A. Tsaplina, Tat'yana I Bogdanova, Tat'yana P Tourova, Marya A Egorova, Elena N Krasil'nikova, Leonid M ZakharchukAbstract:Comparative analysis of 16S rRNA gene sequences, DNA-DNA hybridization data and phenotypic properties revealed that 'Sulfobacillus thermosulfidooxidans subsp. thermotolerans' strain K1 is not a member of the genus Sulfobacillus. Phylogenetically, strain K1 is closely related to unclassified strains of the genus Alicyclobacillus: the 16S rRNA gene sequence of strain K1 is similar to that of Alicyclobacillus sp. AGC-2 (99.6 %), Alicyclobacillus sp. 5C (98.9 %) and Alicyclobacillus sp. CLG (98.6 %) and bacterium GSM (99.1 %). The 16S rRNA gene sequence similarity values for strain K1 and species of the genus Alicyclobacillus with validly published names were in the range 92.1-94.6 %, and for S. thermosulfidooxidans VKM B-1269(T) the value was 87.7 %. Sulfobacillus disulfidooxidans SD-11(T) was also phylogenetically related to strain K1 (92.6 % sequence similarity) and thus belonged to the genus Alicyclobacillus. Chemotaxonomic data, such as the major cell-membrane lipid components of strains K1 and SD-11(T) (omega-alicyclic fatty acids) and the major isoprenoid quinone (menaquinone MK-7) of strain K1, supported the affiliation of strains K1 and SD-11(T) to the genus Alicyclobacillus. Physiological and molecular biological tests allowed genotypic and phenotypic differentiation of strains K1 and SD-11(T) from the nine Alicyclobacillus species with validly published names. The G+C content of the DNA of strain K1 was 48.7+/-0.6 mol%; that of strain SD-11(T) was 53+/-1 mol%. DNA-DNA reassociation studies showed low relatedness (22 %) between strains K1 and SD-11(T), and even lower relatedness (3-5 %) between these strains and Alicyclobacillus acidocaldarius subsp. acidocaldarius ATCC 27009(T), DSM 446(T). DNA reassociation of strains K1 and SD-11(T) with Alicyclobacillus cycloheptanicus DSM 4006(T) gave values of 15 and 21, respectively. Based on the phenotypic and phylogenetic characteristics of strains K1 and SD-11(T), Alicyclobacillus tolerans sp. nov. (type strain, K1(T)=VKM B-2304(T)=DSM 16297(T)) and Alicyclobacillus disulfidooxidans comb. nov. (type strain, SD-11(T)=ATCC 51911(T)=DSM 12064(T)) are proposed.
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Sulfobacillus sibiricus sp. nov., a New Moderately Thermophilic Bacterium
Microbiology, 2003Co-Authors: V. S. Melamud, Tamara F. Kondrat'eva, T. A. Pivovarova, T. V. Kolganova, Tat'yana P Tourova, G. A. Osipov, Anatoly M. Lysenko, G. I. KaravaikoAbstract:In the course of pilot industrial testing of a biohydrometallurgical technology for processing gold-arsenic concentrate obtained from the Nezhdaninskoe ore deposit (East Siberia, Sakha (Yakutiya)), a new gram-positive rod-shaped spore-forming moderately thermophilic bacterium (designated as strain N1) oxidizing Fe2+, S0, and sulfide minerals in the presence of yeast extract (0.02%) was isolated from a dense pulp. Physiologically, strain N1 differs from previously described species of the genus Sulfobacillus in having a somewhat higher optimal growth temperature (55°C). Unlike the type strain of S. thermosulfidooxidans, strain N1 could grow on a medium with 1 mM thiosulfate or sodium tetrathionate as a source of energy only within several passages and failed to grow in the absence of an inorganic energy source on media with sucrose, fructose, glucose, reduced glutathione, alanine, cysteine, sorbitol, sodium acetate, or pyruvate. The G+C content of the DNA of strain N1 was 48.2 mol %. The strain showed 42% homology after DNA–DNA hybridization with the type strain of S. thermosulfidooxidans and 10% homology with the type strain of S. acidophilus. The isolate differed from previously studied strains of S. thermosulfidooxidans in the structure of its chromosomal DNA (determined by the method of pulsed-field gel electrophoresis), which remained stable as growth conditions were changed. According to the results of the 16S rRNA gene analysis, the new strain forms a single cluster with the bacteria of the species Sulfobacillus thermosulfidooxidans (sequence similarity of 97.9–98.6%). Based on these genetic and physiological features, strain N1 is described as a new species Sulfobacillus sibiricus sp. nov.
Xinhua Chen - One of the best experts on this subject based on the ideXlab platform.
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Complete genome analysis of Sulfobacillus acidophilus strain TPY, isolated from a hydrothermal vent in the Pacific Ocean
2016Co-Authors: Yaping Chen, Qian Liu, Xinhua ChenAbstract:Sulfobacillus acidophilus strain TPY is a moderately thermoacidophilic bacterium originally isolated from a hydrothermal vent in the Pacific Ocean. Ferrous iron and sulfur oxidation in acidic environments in strain TPY have been confirmed. Here we report the genome sequence and annotation of the strain TPY, which is the first complete genome of Sulfobacillus acidophilus. Sulfobacillus acidophilus strain TPY, isolated from a hydro-thermal vent in the Pacific Ocean (12°4229N, 104°0201W; water depth, 3,083 m) has been identified as a moderately thermophilic acidophilic bacterium (12). Phylogenetic analyses showed that strain TPY belongs to the species Sulfobacillus acidophilus. The previous experiments proved that TPY could tolerate high temperatures and low pH and that its optimal growth temperature is approximately 50°C and the optimal pH is 1.8 (12). This spore-forming, Gram-positive strain with high GC content has the ability to oxidize elemental sulfur and ferrous ion as electron donors (11)
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Phenol degradation by Sulfobacillus acidophilus TPY via the meta-pathway
Microbiological research, 2016Co-Authors: Wengen Zhou, Hongbo Zhou, Wenbin Guo, Xinhua ChenAbstract:Due to its toxicity and volatility, phenol must be cleared from the environment. Sulfobacillus acidophilus TPY, which was isolated from a hydrothermal vent in the Pacific Ocean as a moderately thermoacidophilic Gram-positive bacterium, was capable of aerobically degrading phenol. This bacterium could tolerate up to 1300mg/L phenol and degrade 100mg/L phenol in 40h completely at 45°C and pH 1.8 with a maximal degradation rate of 2.32mg/L/h at 38h. Genome-wide search revealed that one gene (TPY_3176) and 14 genes clustered together in two regions with locus tags of TPY_0628-0634 and TPY_0640-0646 was proposed to be involved in phenol degradation via the meta-pathway with both the 4-oxalocrotonate branch and the hydrolytic branch. Real-time PCR analysis of S. acidophilus TPY under phenol cultivation condition confirmed the transcription of proposed genes involved in the phenol degradation meta-pathway. Degradation of 3-methylphenol and 2-methylphenol confirmed that the hydrolytic branch was utilised by S. acidophilus TPY. Phylogenetic analysis revealed that S. acidophilus TPY was closely related to sulphate-reducing bacteria and some Gram-positive phenol-degrading bacteria. This was the first report demonstrating the ability of S. acidophilus to degrade phenol and characterising the putative genes involved in phenol metabolism in S. acidophilus TPY.
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Bioleaching of chalcopyrite by defined mixed moderately thermophilic consortium including a marine acidophilic halotolerant bacterium
Bioresource Technology, 2012Co-Authors: Yuguang Wang, Weimin Zeng, Lijuan Zhang, Xinhua Chen, Lijun Su, Junzi Wu, Hongbo ZhouAbstract:Abstract A defined mixed moderately thermophilic consortium including three terrestrial microorganisms ( Leptospirillum ferriphilum , Acidithiobacillus caldus and Ferroplasma thermophilum ) and a marine acidophilic halotolerant bacterium ( Sulfobacillus sp. TPY) was constructed to evaluate its ability for bioleaching of chalcopyrite with the addition of sodium chloride (NaCl), and the community dynamics was monitored by real-time quantitative PCR (qPCR). It was found that Sulfobacillus sp. TPY was able to tolerate 2% (w/v) NaCl, while other three microorganisms were suppressed when the concentration of NaCl was higher than 0.35%. The results suggested that NaCl below certain concentration could improve copper extraction by using pure cultures or the consortium to bioleach chalcopyrite. Community dynamics analysis during bioleaching at 0.1% NaCl showed that Sulfobacillus sp. TPY was predominant species during the whole bioleaching process, L. ferriphilum and A. caldus were less at any time compared with Sulfobacillus sp. TPY. F. thermophilum had never been dominant species even in the final stage.
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Complete Genome Analysis of Sulfobacillus acidophilus Strain TPY, Isolated from a Hydrothermal Vent in the Pacific Ocean
Journal of bacteriology, 2011Co-Authors: Yaping Chen, Qian Liu, Xinhua ChenAbstract:Sulfobacillus acidophilus strain TPY is a moderately thermoacidophilic bacterium originally isolated from a hydrothermal vent in the Pacific Ocean. Ferrous iron and sulfur oxidation in acidic environments in strain TPY have been confirmed. Here we report the genome sequence and annotation of the strain TPY, which is the first complete genome of Sulfobacillus acidophilus.
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application of real time pcr to monitor population dynamics of defined mixed cultures of moderate thermophiles involved in bioleaching of chalcopyrite
Applied Microbiology and Biotechnology, 2009Co-Authors: Rubing Zhang, Xinhua Chen, Houguo Ji, Hongbo ZhouAbstract:To compare oxidative dissolution rates of chalcopyrite by different consortia of moderately thermophilic acidophiles, various defined mixed cultures of three bacteria Acidithiobacillus caldus s2, Leptospirillum ferriphilum YSK, and Sulfobacillus sp. LN and one archaeon Ferroplasma thermophilum L1 were studied in batch shake flask cultures incubated at 45 °C. Chalcopyrite dissolution was determined by measuring variations of soluble copper, ferric iron, and pH. Microbial population dynamics involved in bioleaching process were monitored using real-time quantitative polymerase chain reaction (PCR) technology. The complex consortia containing both chemoautotrophic (L. ferriphilum and At. caldus) and chemomixotrophic (Sulfobacillus LN and F. thermophilum) moderate thermophiles were found to be the most efficient in all of those tested. Mutualistic interactions between physiologically distinct moderately thermophilic acidophiles, involving transformations of iron and sulfur and transfer of organic compound, were considered to play a critical role in promoting chalcopyrite dissolution. The real-time PCR assay was reliable to analyze population dynamics of moderate thermophiles in bioleaching systems, and the analysis results were consistent with physiological characteristics of these strains.
Paul R Norris - One of the best experts on this subject based on the ideXlab platform.
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ribulose bisphosphate carboxylase activity and a calvin cycle gene cluster in Sulfobacillus species
Microbiology, 2007Co-Authors: Paul E Caldwell, Marti R Maclea, Paul R NorrisAbstract:The Calvin–Benson–Bassham (CBB) cycle has been extensively studied in proteobacteria, cyanobacteria, algae and plants, but hardly at all in Gram-positive bacteria. Some characteristics of ribulose bisphosphate carboxylase/oxygenase (RuBisCO) and a cluster of potential CBB cycle genes in a Gram-positive bacterium are described in this study with two species of Sulfobacillus (Gram-positive, facultatively autotrophic, mineral sulfide-oxidizing acidophiles). In contrast to the Gram-negative, iron-oxidizing acidophile Acidithiobacillus ferrooxidans, Sulfobacillus thermosulfidooxidans grew poorly autotrophically unless the CO2 concentration was enhanced over that in air. However, the RuBisCO of each organism showed similar affinities for CO2 and for ribulose 1,5-bisphosphate, and similar apparent derepression of activity under CO2 limitation. The red-type, form I RuBisCO of Sulfobacillus acidophilus was confirmed as closely related to that of the anoxygenic phototroph Oscillochloris trichoides. Eight genes potentially involved in the CBB cycle in S. acidophilus were clustered in the order cbbA, cbbP, cbbE, cbbL, cbbS, cbbX, cbbG and cbbT.
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a novel acidimicrobium species in continuous cultures of moderately thermophilic mineral sulfide oxidizing acidophiles
Applied and Environmental Microbiology, 2007Co-Authors: Adam A Cleaver, Nicolas P Burton, Paul R NorrisAbstract:A novel species of Acidimicrobium appeared to be the predominant ferrous iron oxidizer in a mixed culture that effected the continuous, efficient extraction of nickel from a mineral concentrate at 49°C, but it was not isolated in pure culture. It outcompeted Acidimicrobium ferrooxidans, which was expected to have a major role in iron oxidation in reactors gassed with air, and was outnumbered at 49°C only by the sulfur-oxidizing Acidithiobacillus caldus. Sulfobacillus species were expected to compete with Acidimicrobium species when culture aeration was enriched with carbon dioxide, but they were a minor component of the populations with and without this enrichment. Sulfobacillus thermosulfidooxidans replaced the Acidimicrobium species and Acidithiobacillus caldus when the temperature was increased to 55°C.
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characteristics of Sulfobacillus acidophilus sp nov and other moderately thermophilic mineral sulphide oxidizing bacteria
Microbiology, 1996Co-Authors: Paul R Norris, Darren A Clark, Jonathan P Owen, Sara WaterhouseAbstract:Several isolates of Gram-positive, acidophilic, moderately thermophilic, ferrous-iron- and mineral-sulphide-oxidizing bacteria were examined to establish unequivocally the characteristics of Sulfobacillus-like bacteria. Two species were evident: Sulfobacillus thermosulfidooxidans with 48-50 mol% G + C and Sulfobacillus acidophilus sp. nov. with 55-57 mol% G + C. Both species grew autotrophically and mixotrophically on ferrous iron, on elemental sulphur in the presence of yeast extract, and heterotrophically on yeast extract. Autotrophic growth on sulphur was consistently obtained only with S. acidophilus.
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Acidimicrobium ferrooxidans gen. nov., sp. nov.: mixed-culture ferrous iron oxidation with Sulfobacillus species
Microbiology, 1996Co-Authors: Darren A Clark, Paul R NorrisAbstract:A new species of ferrous-iron-oxidizing, moderately thermophilic, acidophilic bacteria, Acidimicrobium ferrooxidans, has been described. Two isolates of the species differed only in the tendency of one, previously known as strain TH3, to grow in filaments. The chromosomal DNA base composition is between 67 and 69 mol% G + C. The capacity of this species to fix CO2 from air was greater than that of iron-oxidizing thermoacidophiles of the genus Sulfobacillus, which required an enhanced CO2 concentration for optimum autotrophic growth. Under air, ferrous iron oxidation in mixed cultures of A. ferrooxidans with either Sulfobacillus thermosulfidooxidans or Sulfobacillus acidophilus was more extensive than in pure cultures of these three strains. The greater part of ferrous iron oxidation in mixed cultures probably resulted from activity of the Sulfobacillus species, which possess a greater tolerance of ferric iron, and which presumably grew mixotrophically utilizing organic compounds from A. ferrooxidans.
