The Experts below are selected from a list of 376407 Experts worldwide ranked by ideXlab platform
Stephen J. Moss - One of the best experts on this subject based on the ideXlab platform.
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modulation of neuronal activity by phosphorylation of the k cl cotransporter kcc2
Trends in Neurosciences, 2013Co-Authors: Stephen J. Moss, Kristopher T Kahle, Tarek Z Deeb, Martin Puskarjov, Liliya Silayeva, Bo Liang, Kai KailaAbstract:The K–Cl cotransporter KCC2 establishes the low intraneuronal Cl – levels required for the hyperpolarizing inhibitory postsynaptic potentials mediated by ionotropic γ-aminobutyric acid receptors (GABA A Rs) and glycine receptors (GlyRs). Decreased KCC2-mediated Cl – extrusion and impaired hyperpolarizing GABA A R- and/or GlyR-mediated currents have been implicated in epilepsy, neuropathic pain, and spasticity. Recent evidence suggests that the intrinsic ion transport rate, cell Surface Stability, and plasmalemmal trafficking of KCC2 are rapidly and reversibly modulated by the (de)phosphorylation of critical serine, threonine, and tyrosine residues in the C terminus of this protein. Alterations in KCC2 phosphorylation have been associated with impaired KCC2 function in several neurological diseases. Targeting KCC2 phosphorylation directly or indirectly via upstream regulatory kinases might be a novel strategy to modulate GABA- and/or glycinergic signaling for therapeutic benefit.
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tyrosine phosphorylation regulates the membrane trafficking of the potassium chloride co transporter kcc2
Molecular and Cellular Neuroscience, 2010Co-Authors: Henry H C Lee, Stephen J. Moss, Rachel JurdAbstract:The activity of the neuronal-specific potassium chloride co-transporter KCC2 allows neurons to maintain low intracellular Cl(-) concentrations. These low Cl(-) concentrations are critical in mediating fast synaptic inhibition upon the activation of Cl(-)-permeable ligand-gated ion channels such as type A gamma-aminobutyric acid receptors (GABA(A)Rs). Deficits in KCC2 functional expression thus play central roles in the etiology of epilepsy and ischemia. It is emerging that KCC2 is phosphorylated on tyrosine residues, but the molecular substrates for this covalent modification within KCC2 and its functional significance remain poorly understood. Here we demonstrate that in HEK-293 cells the principal sites of tyrosine phosphorylation within KCC2 are residues 903 and 1087 (Y903/1087), which lie within the major C-terminal intracellular domain of KCC2. Phosphorylation of Y903/1087 decreases the cell Surface Stability of KCC2 principally by enhancing their lysozomal degradation. We further demonstrate that in cultured hippocampal neurons prolonged activation of muscarinic acetylcholine receptors (mAChRs) enhances KCC2 tyrosine phosphorylation and lysozomal degradation. Consistent with our in vitro studies, induction of status epilepticus (SE) in mice using pilocarpine, a mAChR agonist, induces large deficits in the cell Surface Stability of KCC2 together with enhanced tyrosine phosphorylation. Tyrosine phosphorylation of KCC2 is thus likely to play a key role in regulating the degradation of KCC2, a process that may be responsible for pathological losses of KCC2 function that are evident in SE and other forms of epilepsy.
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direct protein kinase c dependent phosphorylation regulates the cell Surface Stability and activity of the potassium chloride cotransporter kcc2
Journal of Biological Chemistry, 2007Co-Authors: Henry H C Lee, Stephen J. Moss, Joshua A Walker, Jeffery R Williams, Richard J Goodier, John A PayneAbstract:Abstract The potassium chloride cotransporter KCC2 plays a major role in the maintenance of transmembrane chloride potential in mature neurons; thus KCC2 activity is critical for hyperpolarizing membrane currents generated upon the activation of γ-aminobutyric acid type A and glycine (Gly) receptors that underlie fast synaptic inhibition in the adult central nervous system. However, to date an understanding of the cellular mechanism that neurons use to modulate the functional expression of KCC2 remains rudimentary. Using Escherichia coli expression coupled with in vitro kinase assays, we first established that protein kinase C (PKC) can directly phosphorylate serine 940 (Ser940) within the C-terminal cytoplasmic domain of KCC2. We further demonstrated that Ser940 is the major site for PKC-dependent phosphorylation for full-length KCC2 molecules when expressed in HEK-293 cells. Phosphorylation of Ser940 increased the cell Surface Stability of KCC2 in this system by decreasing its rate of internalization from the plasma membrane. Coincident phosphorylation of Ser940 increased the rate of ion transport by KCC2. It was further evident that phosphorylation of endogenous KCC2 in cultured hippocampal neurons is regulated by PKC-dependent activity. Moreover, in keeping with our recombinant studies, enhancing PKC-dependent phosphorylation increased the targeting of KCC2 to the neuronal cell Surface. Our studies thus suggest that PKC-dependent phosphorylation of KCC2 may play a central role in modulating both the functional expression of this critical transporter in the brain and the strength of synaptic inhibition.
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studying the localization Surface Stability and endocytosis of neurotransmitter receptors by antibody labeling and biotinylation approaches
In: Kittler JT and Moss SJ (eds.) The dynamic synapse. CRC (2006), 2006Co-Authors: Lorena I Arancibiacarcamo, Benjamin P Fairfax, Stephen J. Moss, Josef T KittlerAbstract:This book provides researchers interested in studying the development and plasticity of synapses and the regulation of ion channels and receptors with detailed ...
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direct interaction of n ethylmaleimide sensitive factor with gabaa receptor β subunits
Molecular and Cellular Neuroscience, 2005Co-Authors: Hidefumi Goto, Stephen J. Moss, Miho Terunuma, Takashi Kanematsu, Yoshio Misumi, Masato HirataAbstract:GABAA receptors mediate most of the fast inhibitory neurotransmission in the brain, and are believed to be composed mainly of α, β, and γ subunits. It has been shown that GABAA receptors interact with a number of binding partners that act to regulate both receptor function and cell Surface Stability. Here, we reveal that GABAA receptors interact directly with N-ethylmaleimide-sensitive factor (NSF), a critical regulator of vesicular dependent protein trafficking, as measured by in vitro protein binding and co-immunoprecipitation assays. In addition, we established that NSF interacts with residues 395–415 of the receptor β subunits and co-localizes with GABAA receptors in hippocampal neurons. We also established that NSF can regulate GABAA receptor cell Surface expression depending upon residues 395–415 in the β3 subunit. Together, our results suggest an important role for NSF activity in regulating the cell Surface Stability of GABAA receptors.
Susan I Gerber - One of the best experts on this subject based on the ideXlab platform.
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aerosol and Surface Stability of sars cov 2 as compared with sars cov 1
The New England Journal of Medicine, 2020Co-Authors: Neeltje Van Doremalen, Trenton Bushmaker, Dylan H Morris, Myndi Holbrook, Amandine Gamble, Brandi N Williamson, Azaibi Tamin, Jennifer L Harcourt, Natalie J Thornburg, Susan I GerberAbstract:Aerosol and Surface Stability of SARS-CoV-2 In this research letter, investigators report on the Stability of SARS-CoV-2 and SARS-CoV-1 under experimental conditions. The viability of the two virus...
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aerosol and Surface Stability of hcov 19 sars cov 2 compared to sars cov 1
medRxiv, 2020Co-Authors: Neeltje Van Doremalen, Trenton Bushmaker, Dylan H Morris, Myndi Holbrook, Amandine Gamble, Brandi N Williamson, Azaibi Tamin, Jennifer L Harcourt, Natalie J Thornburg, Susan I GerberAbstract:A novel human coronavirus, now named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, referred to as HCoV-19 here) that emerged in Wuhan, China in late 2019 is now causing a pandemic. Here, we analyze the aerosol and Surface Stability of HCoV-19 and compare it with SARS-CoV-1, the most closely related human coronavirus.2 We evaluated the Stability of HCoV-19 and SARS-CoV-1 in aerosols and on different Surfaces and estimated their decay rates using a Bayesian regression model
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aerosol and Surface Stability of hcov 19 sars cov 2 compared to sars cov 1
medRxiv, 2020Co-Authors: Neeltje Van Doremalen, Trenton Bushmaker, Dylan H Morris, Amandine Gamble, Brandi N Williamson, Azaibi Tamin, Jennifer L Harcourt, Natalie J Thornburg, Myndi G Holbrook, Susan I GerberAbstract:To the Editor A novel human coronavirus, now named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, referred to as HCoV-19 here) that emerged in Wuhan, China in late 2019 is now causing a pandemic1. Here, we analyze the aerosol and Surface Stability of HCoV-19 and compare it with SARS-CoV-1, the most closely related human coronavirus.2 We evaluated the Stability of HCoV-19 and SARS-CoV-1 in aerosols and on different Surfaces and estimated their decay rates using a Bayesian regression model (see Supplementary Appendix). All experimental measurements are reported as mean across 3 replicates.
Neeltje Van Doremalen - One of the best experts on this subject based on the ideXlab platform.
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aerosol and Surface Stability of sars cov 2 as compared with sars cov 1
The New England Journal of Medicine, 2020Co-Authors: Neeltje Van Doremalen, Trenton Bushmaker, Dylan H Morris, Myndi Holbrook, Amandine Gamble, Brandi N Williamson, Azaibi Tamin, Jennifer L Harcourt, Natalie J Thornburg, Susan I GerberAbstract:Aerosol and Surface Stability of SARS-CoV-2 In this research letter, investigators report on the Stability of SARS-CoV-2 and SARS-CoV-1 under experimental conditions. The viability of the two virus...
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aerosol and Surface Stability of hcov 19 sars cov 2 compared to sars cov 1
medRxiv, 2020Co-Authors: Neeltje Van Doremalen, Trenton Bushmaker, Dylan H Morris, Myndi Holbrook, Amandine Gamble, Brandi N Williamson, Azaibi Tamin, Jennifer L Harcourt, Natalie J Thornburg, Susan I GerberAbstract:A novel human coronavirus, now named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, referred to as HCoV-19 here) that emerged in Wuhan, China in late 2019 is now causing a pandemic. Here, we analyze the aerosol and Surface Stability of HCoV-19 and compare it with SARS-CoV-1, the most closely related human coronavirus.2 We evaluated the Stability of HCoV-19 and SARS-CoV-1 in aerosols and on different Surfaces and estimated their decay rates using a Bayesian regression model
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aerosol and Surface Stability of hcov 19 sars cov 2 compared to sars cov 1
medRxiv, 2020Co-Authors: Neeltje Van Doremalen, Trenton Bushmaker, Dylan H Morris, Amandine Gamble, Brandi N Williamson, Azaibi Tamin, Jennifer L Harcourt, Natalie J Thornburg, Myndi G Holbrook, Susan I GerberAbstract:To the Editor A novel human coronavirus, now named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, referred to as HCoV-19 here) that emerged in Wuhan, China in late 2019 is now causing a pandemic1. Here, we analyze the aerosol and Surface Stability of HCoV-19 and compare it with SARS-CoV-1, the most closely related human coronavirus.2 We evaluated the Stability of HCoV-19 and SARS-CoV-1 in aerosols and on different Surfaces and estimated their decay rates using a Bayesian regression model (see Supplementary Appendix). All experimental measurements are reported as mean across 3 replicates.
J H Booske - One of the best experts on this subject based on the ideXlab platform.
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work function and Stability of adsorbed ba o and ba o species on an array of tungsten Surfaces
International Vacuum Electronics Conference, 2018Co-Authors: Ryan Jacobs, Dane Morgan, J H BooskeAbstract:In this work, we have used Density Functional Theory to explore the effect of O, Ba and Ba-O adsorbates on W Surfaces to assess the impact of these adsorbates on the work function and Surface Stability for eight different W Surface orientations. A discussion of the impact of different Surface adsorbates on the Surface Stability and work function is presented, together with newly discovered stable Ba-O arrangements on the most stable W Surfaces. Finally, we discuss the potential role of high index Surfaces in electron emission, and the possible impact of W diffusion kinetics on the prevalence of high index Surfaces.
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work function and Surface Stability of tungsten based thermionic electron emission cathodes
arXiv: Materials Science, 2017Co-Authors: Ryan Jacobs, Dane Morgan, J H BooskeAbstract:Materials that exhibit a low work function and therefore easily emit electrons into vacuum form the basis of electronic devices used in applications ranging from satellite communications to thermionic energy conversion. W-Ba-O is the canonical materials system that functions as the thermionic electron emitter used commercially in a range of high power electron devices. However, the work functions, Surface Stability, and kinetic characteristics of a polycrystalline W emitter Surface are still not well understood or characterized. In this study, we examined the work function and Surface Stability of the eight lowest index Surfaces of the W-Ba-O system using Density Functional Theory methods. We found that under the typical thermionic cathode operating conditions of high temperature and low oxygen partial pressure, the most stable Surface adsorbates are Ba-O species with compositions in the range of Ba0.125O to Ba0.25O per Surface W atom, with O passivating all dangling W bonds and Ba creating work function-lowering Surface dipoles. Wulff construction analysis reveals that the presence of O and Ba significantly alters the Surface energetics and changes the proportions of Surface facets present under equilibrium conditions. Analysis of previously published data on W sintering kinetics suggests that fine W particles in the size range of 100-500 nm may be at or near equilibrium during cathode synthesis, and thus may exhibit Surface orientation fractions well-described by the calculated Wulff construction.
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work function and Surface Stability of tungsten based thermionic electron emission cathodes
APL Materials, 2017Co-Authors: Ryan Jacobs, Dane Morgan, J H BooskeAbstract:Materials that exhibit a low work function and therefore easily emit electrons into vacuum form the basis of electronic devices used in applications ranging from satellite communications to thermionic energy conversion. W–Ba–O is the canonical materials system that functions as the thermionic electron emitter commercially used in a range of high-power electron devices. However, the work functions, Surface Stability, and kinetic characteristics of a polycrystalline W emitter Surface are still not well understood or characterized. In this study, we examined the work function and Surface Stability of the eight lowest index Surfaces of the W–Ba–O system using density functional theory methods. We found that under the typical thermionic cathode operating conditions of high temperature and low oxygen partial pressure, the most stable Surface adsorbates are Ba–O species with compositions in the range of Ba0.125O–Ba0.25O per Surface W atom, with O passivating all dangling W bonds and Ba creating work function-low...
Amandine Gamble - One of the best experts on this subject based on the ideXlab platform.
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aerosol and Surface Stability of sars cov 2 as compared with sars cov 1
The New England Journal of Medicine, 2020Co-Authors: Neeltje Van Doremalen, Trenton Bushmaker, Dylan H Morris, Myndi Holbrook, Amandine Gamble, Brandi N Williamson, Azaibi Tamin, Jennifer L Harcourt, Natalie J Thornburg, Susan I GerberAbstract:Aerosol and Surface Stability of SARS-CoV-2 In this research letter, investigators report on the Stability of SARS-CoV-2 and SARS-CoV-1 under experimental conditions. The viability of the two virus...
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aerosol and Surface Stability of hcov 19 sars cov 2 compared to sars cov 1
medRxiv, 2020Co-Authors: Neeltje Van Doremalen, Trenton Bushmaker, Dylan H Morris, Myndi Holbrook, Amandine Gamble, Brandi N Williamson, Azaibi Tamin, Jennifer L Harcourt, Natalie J Thornburg, Susan I GerberAbstract:A novel human coronavirus, now named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, referred to as HCoV-19 here) that emerged in Wuhan, China in late 2019 is now causing a pandemic. Here, we analyze the aerosol and Surface Stability of HCoV-19 and compare it with SARS-CoV-1, the most closely related human coronavirus.2 We evaluated the Stability of HCoV-19 and SARS-CoV-1 in aerosols and on different Surfaces and estimated their decay rates using a Bayesian regression model
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aerosol and Surface Stability of hcov 19 sars cov 2 compared to sars cov 1
medRxiv, 2020Co-Authors: Neeltje Van Doremalen, Trenton Bushmaker, Dylan H Morris, Amandine Gamble, Brandi N Williamson, Azaibi Tamin, Jennifer L Harcourt, Natalie J Thornburg, Myndi G Holbrook, Susan I GerberAbstract:To the Editor A novel human coronavirus, now named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, referred to as HCoV-19 here) that emerged in Wuhan, China in late 2019 is now causing a pandemic1. Here, we analyze the aerosol and Surface Stability of HCoV-19 and compare it with SARS-CoV-1, the most closely related human coronavirus.2 We evaluated the Stability of HCoV-19 and SARS-CoV-1 in aerosols and on different Surfaces and estimated their decay rates using a Bayesian regression model (see Supplementary Appendix). All experimental measurements are reported as mean across 3 replicates.
